JP7221199B2 - マルチプレックスｐｃｒの実施方法 - Google Patents

マルチプレックスｐｃｒの実施方法 Download PDF

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JP7221199B2
JP7221199B2 JP2019514294A JP2019514294A JP7221199B2 JP 7221199 B2 JP7221199 B2 JP 7221199B2 JP 2019514294 A JP2019514294 A JP 2019514294A JP 2019514294 A JP2019514294 A JP 2019514294A JP 7221199 B2 JP7221199 B2 JP 7221199B2
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moiety
nucleic acid
oligonucleotide
tag
quenching
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JP2019532628A5 (enExample
JP2019532628A (ja
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コズロフ イゴル
グプタ アマー
サイキ ランドール
ツァン アリソン
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F Hoffmann La Roche AG
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    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
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    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
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    • C12Q2531/00—Reactions of nucleic acids characterised by
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    • C12Q2537/00—Reactions characterised by the reaction format or use of a specific feature
    • C12Q2537/10—Reactions characterised by the reaction format or use of a specific feature the purpose or use of
    • C12Q2537/143—Multiplexing, i.e. use of multiple primers or probes in a single reaction, usually for simultaneously analyse of multiple analysis
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    • C12Q2561/00—Nucleic acid detection characterised by assay method
    • C12Q2561/113—Real time assay
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    • C12Q2565/00—Nucleic acid analysis characterised by mode or means of detection
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    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/16—Primer sets for multiplex assays

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  • Chemical & Material Sciences (AREA)
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  • Organic Chemistry (AREA)
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  • Proteomics, Peptides & Aminoacids (AREA)
  • Engineering & Computer Science (AREA)
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  • Chemical Kinetics & Catalysis (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
JP2019514294A 2016-09-15 2017-09-15 マルチプレックスｐｃｒの実施方法 Active JP7221199B2 (ja)

Applications Claiming Priority (7)

Application Number Priority Date Filing Date Title
US201662395325P 2016-09-15 2016-09-15
US62/395,325 2016-09-15
US201662435595P 2016-12-16 2016-12-16
US62/435,595 2016-12-16
US201762536871P 2017-07-25 2017-07-25
US62/536,871 2017-07-25
PCT/EP2017/073288 WO2018050824A1 (en) 2016-09-15 2017-09-15 Methods for performing multiplexed pcr

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JP2019532628A JP2019532628A (ja) 2019-11-14
JP2019532628A5 JP2019532628A5 (enExample) 2020-10-22
JP7221199B2 true JP7221199B2 (ja) 2023-02-20

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JP2019514294A Active JP7221199B2 (ja) 2016-09-15 2017-09-15 マルチプレックスｐｃｒの実施方法

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US (3) US11028433B2 (enExample)
EP (2) EP3512959B1 (enExample)
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KR (2) KR102468174B1 (enExample)
CN (2) CN109689889A (enExample)
AU (2) AU2017328613B2 (enExample)
CA (2) CA3033250C (enExample)
DK (1) DK3512959T3 (enExample)
ES (2) ES2893411T3 (enExample)
WO (2) WO2018050824A1 (enExample)

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JP2024522640A (ja) 2021-06-25 2024-06-21 エフ． ホフマン－ラ ロシュ アーゲー 高感度で温度多重化ｐｃｒを行うための方法
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