CN109689884A - 用于进行多重实时pcr的方法 - Google Patents
用于进行多重实时pcr的方法 Download PDFInfo
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- CN109689884A CN109689884A CN201780056666.0A CN201780056666A CN109689884A CN 109689884 A CN109689884 A CN 109689884A CN 201780056666 A CN201780056666 A CN 201780056666A CN 109689884 A CN109689884 A CN 109689884A
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- nucleic acid
- label segment
- quenching
- target nucleic
- oligonucleotide probe
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- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/686—Polymerase chain reaction [PCR]
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- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6804—Nucleic acid analysis using immunogens
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- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6811—Selection methods for production or design of target specific oligonucleotides or binding molecules
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- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6816—Hybridisation assays characterised by the detection means
- C12Q1/6818—Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer
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- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6816—Hybridisation assays characterised by the detection means
- C12Q1/6823—Release of bound markers
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
- C12Q1/6837—Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips
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- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
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- C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
- C12Q2525/10—Modifications characterised by
- C12Q2525/161—Modifications characterised by incorporating target specific and non-target specific sites
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- C12Q2527/00—Reactions demanding special reaction conditions
- C12Q2527/107—Temperature of melting, i.e. Tm
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- C12Q2531/00—Reactions of nucleic acids characterised by
- C12Q2531/10—Reactions of nucleic acids characterised by the purpose being amplify/increase the copy number of target nucleic acid
- C12Q2531/113—PCR
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- C12Q2537/00—Reactions characterised by the reaction format or use of a specific feature
- C12Q2537/10—Reactions characterised by the reaction format or use of a specific feature the purpose or use of
- C12Q2537/143—Multiplexing, i.e. use of multiple primers or probes in a single reaction, usually for simultaneously analyse of multiple analysis
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- C12Q2561/00—Nucleic acid detection characterised by assay method
- C12Q2561/113—Real time assay
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- C12Q2565/00—Nucleic acid analysis characterised by mode or means of detection
- C12Q2565/10—Detection mode being characterised by the assay principle
- C12Q2565/101—Interaction between at least two labels
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- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/16—Primer sets for multiplex assays
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- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Analytical Chemistry (AREA)
- Genetics & Genomics (AREA)
- Immunology (AREA)
- Molecular Biology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Pathology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (7)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201662395325P | 2016-09-15 | 2016-09-15 | |
| US62/395325 | 2016-09-15 | ||
| US201662435595P | 2016-12-16 | 2016-12-16 | |
| US62/435595 | 2016-12-16 | ||
| US201762536871P | 2017-07-25 | 2017-07-25 | |
| US62/536871 | 2017-07-25 | ||
| PCT/EP2017/073293 WO2018050828A1 (en) | 2016-09-15 | 2017-09-15 | Methods for performing multiplexed real-time pcr |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN109689884A true CN109689884A (zh) | 2019-04-26 |
Family
ID=59901522
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201780056666.0A Pending CN109689884A (zh) | 2016-09-15 | 2017-09-15 | 用于进行多重实时pcr的方法 |
| CN201780056599.2A Pending CN109689889A (zh) | 2016-09-15 | 2017-09-15 | 用于进行多重pcr的方法 |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201780056599.2A Pending CN109689889A (zh) | 2016-09-15 | 2017-09-15 | 用于进行多重pcr的方法 |
Country Status (10)
| Country | Link |
|---|---|
| US (3) | US11034997B2 (enExample) |
| EP (2) | EP3512959B1 (enExample) |
| JP (2) | JP7063886B2 (enExample) |
| KR (2) | KR102468174B1 (enExample) |
| CN (2) | CN109689884A (enExample) |
| AU (2) | AU2017328613B2 (enExample) |
| CA (2) | CA3033250C (enExample) |
| DK (1) | DK3512959T3 (enExample) |
| ES (2) | ES2933176T3 (enExample) |
| WO (2) | WO2018050824A1 (enExample) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111534518A (zh) * | 2020-05-18 | 2020-08-14 | 纳昂达(南京)生物科技有限公司 | 通用封闭序列及其应用 |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| WO2022269023A1 (en) | 2021-06-25 | 2022-12-29 | F. Hoffmann-La Roche Ag | Methods for performing temperature multiplexed pcr with increased sensitivity |
| JP7063886B2 (ja) | 2016-09-15 | 2022-05-09 | エフ.ホフマン-ラ ロシュ アーゲー | マルチプレックスリアルタイムpcrを実施する方法 |
| RU2665631C2 (ru) * | 2017-01-25 | 2018-09-03 | Общество с ограниченной ответственностью "Секвойя Дженетикс" | Способ специфической идентификации последовательностей днк |
| US20190093155A1 (en) * | 2017-05-25 | 2019-03-28 | Roche Molecular Systems, Inc. | Multiplex Nucleic Acid Amplification Assay |
| CN111629831A (zh) | 2018-01-22 | 2020-09-04 | 卢米耐克斯公司 | 用于离散解链分析的方法和组合物 |
| US20190292596A1 (en) | 2018-03-21 | 2019-09-26 | Roche Molecular Systems, Inc. | Modified nucleoside phosphates with high thermal stability |
| AU2019255933B2 (en) | 2018-04-20 | 2022-08-25 | Seegene, Inc. . | Method and apparatus for detecting a plurality of target nucleic acid sequences in sample |
| US11384376B2 (en) | 2018-05-31 | 2022-07-12 | Roche Molecular Systems, Inc. | Reagents and methods for post-synthetic modification of nucleic acids |
| WO2020031156A1 (en) | 2018-08-09 | 2020-02-13 | Speedx Pty Ltd | Multiplex detection of nucleic acids |
| US11371080B2 (en) | 2018-11-30 | 2022-06-28 | Luminex Corporation | Methods and probes for performing PCR with melt analysis for increased multiplexing |
| EP3899035B1 (en) * | 2018-12-20 | 2025-04-16 | F. Hoffmann-La Roche AG | Detection of target nucleic acid by solid-phase molography |
| US11441167B2 (en) | 2019-11-20 | 2022-09-13 | Roche Molecular Systems, Inc. | Compositions and methods for rapid identification and phenotypic antimicrobial susceptibility testing of bacteria and fungi |
| CN114787286A (zh) | 2019-12-09 | 2022-07-22 | 豪夫迈·罗氏有限公司 | 双阳离子荧光染料 |
| CA3181184A1 (en) * | 2020-06-30 | 2020-10-15 | Speedx Pty Ltd | Multiplex detection of nucleic acids using mixed reporters |
| CN112195221B (zh) * | 2020-09-13 | 2021-08-06 | 武汉纳诺德医疗科技有限公司 | 一种多温度单荧光通道多重实时荧光定量pcr仪及其检测方法 |
| AU2021343471A1 (en) * | 2020-09-16 | 2023-05-04 | Bruker Spatial Biology, Inc. | Chemical compositions and methods of using the same |
| JP2024500169A (ja) | 2020-12-22 | 2024-01-04 | エフ. ホフマン-ラ ロシュ アーゲー | 大ストークスシフト蛍光色素を使用して多重化リアルタイムpcrを行うための方法 |
| JP7641445B2 (ja) | 2021-09-30 | 2025-03-06 | ジェン-プローブ・インコーポレーテッド | 温度で選択可能なfretカセットシグナル伝達 |
| WO2024002924A2 (en) | 2022-06-28 | 2024-01-04 | F. Hoffmann-La Roche Ag | Fluorescent dyes with large stokes shift |
| WO2024106890A1 (ko) * | 2022-11-16 | 2024-05-23 | 주식회사 씨젠 | 퀀칭-pto 절단 및 연장(quenching-pto cleavage and extension; q-ptoce) 어세이에 의한 타겟 핵산의 검출 |
| AR132405A1 (es) * | 2023-04-19 | 2025-06-25 | Consejo Nacional De Investigaciones Cientificas Y Tecn Conicet | Métodos y sistemas para seleccionar biomarcadores basados en metilación del adn para la detección de cáncer colorrectal |
| AU2024220029A1 (en) * | 2023-05-15 | 2024-11-28 | Speedx Pty Ltd | Target detection using temperature controlled probes |
| WO2025053620A1 (ko) * | 2023-09-08 | 2025-03-13 | 주식회사 씨젠 | 3개의 프로브를 이용하여 타겟 핵산을 검출하는 방법 |
| WO2025141105A1 (en) | 2023-12-27 | 2025-07-03 | bioMérieux | Specific probes for the detection of nucleic acids, methods and uses |
| EP4606908A1 (en) | 2024-02-21 | 2025-08-27 | Roche Diagnostics GmbH | Method of increasing the number of usable channels in multiplex pcr |
Citations (4)
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| WO2010013017A1 (en) * | 2008-07-31 | 2010-02-04 | Oxitec Limited | Multiplex amplification and detection |
| US20140272955A1 (en) * | 2013-03-15 | 2014-09-18 | Roche Molecular Systems, Inc. | Nucleic acid target identification by strucutre based probe cleavage |
| US20150072887A1 (en) * | 2012-02-02 | 2015-03-12 | Seegene, Inc. | Detection of target nucleic acid sequence by pto cleavage and extension-dependent signaling oligonucleotide hybridization assay |
| WO2016101959A1 (en) * | 2014-12-22 | 2016-06-30 | Anapa Biotech A/S | Dual quenching assay for multiplex detection of target nucleic acids |
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