JP7037504B2 - 生体液からの細胞外小胞の単離及びセルフリーdnaの同時単離のための自動及び手動方法 - Google Patents
生体液からの細胞外小胞の単離及びセルフリーdnaの同時単離のための自動及び手動方法 Download PDFInfo
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- JP7037504B2 JP7037504B2 JP2018559313A JP2018559313A JP7037504B2 JP 7037504 B2 JP7037504 B2 JP 7037504B2 JP 2018559313 A JP2018559313 A JP 2018559313A JP 2018559313 A JP2018559313 A JP 2018559313A JP 7037504 B2 JP7037504 B2 JP 7037504B2
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
- C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H1/00—Processes for the preparation of sugar derivatives
- C07H1/06—Separation; Purification
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Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2022033452A JP7354327B2 (ja) | 2016-05-13 | 2022-03-04 | 生体液からの細胞外小胞の単離及びセルフリーdnaの同時単離のための自動及び手動方法 |
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201662336203P | 2016-05-13 | 2016-05-13 | |
| US62/336,203 | 2016-05-13 | ||
| PCT/US2017/032719 WO2017197399A1 (en) | 2016-05-13 | 2017-05-15 | Automated and manual methods for isolation of extracellular vesicles and co-isolation of cell-free dna from biofluids |
Related Child Applications (1)
| Application Number | Title | Priority Date | Filing Date |
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| JP2022033452A Division JP7354327B2 (ja) | 2016-05-13 | 2022-03-04 | 生体液からの細胞外小胞の単離及びセルフリーdnaの同時単離のための自動及び手動方法 |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2019521322A JP2019521322A (ja) | 2019-07-25 |
| JP2019521322A5 JP2019521322A5 (cg-RX-API-DMAC7.html) | 2020-07-02 |
| JP7037504B2 true JP7037504B2 (ja) | 2022-03-16 |
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| Application Number | Title | Priority Date | Filing Date |
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| JP2018559313A Active JP7037504B2 (ja) | 2016-05-13 | 2017-05-15 | 生体液からの細胞外小胞の単離及びセルフリーdnaの同時単離のための自動及び手動方法 |
| JP2022033452A Active JP7354327B2 (ja) | 2016-05-13 | 2022-03-04 | 生体液からの細胞外小胞の単離及びセルフリーdnaの同時単離のための自動及び手動方法 |
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| Application Number | Title | Priority Date | Filing Date |
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| JP2022033452A Active JP7354327B2 (ja) | 2016-05-13 | 2022-03-04 | 生体液からの細胞外小胞の単離及びセルフリーdnaの同時単離のための自動及び手動方法 |
Country Status (6)
| Country | Link |
|---|---|
| US (2) | US10808240B2 (cg-RX-API-DMAC7.html) |
| EP (2) | EP4079853A1 (cg-RX-API-DMAC7.html) |
| JP (2) | JP7037504B2 (cg-RX-API-DMAC7.html) |
| CN (1) | CN109642229A (cg-RX-API-DMAC7.html) |
| AU (1) | AU2017263886B2 (cg-RX-API-DMAC7.html) |
| WO (1) | WO2017197399A1 (cg-RX-API-DMAC7.html) |
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| US11268085B2 (en) * | 2014-05-27 | 2022-03-08 | Exosome Diagnostics, Inc. | Methods for isolating microvesicles and extracting nucleic acids from biological samples |
| AU2017263886B2 (en) * | 2016-05-13 | 2023-11-09 | Exosome Diagnostics, Inc. | Automated and manual methods for isolation of extracellular vesicles and co-isolation of cell-free DNA from biofluids |
| RU2748234C2 (ru) | 2016-12-23 | 2021-05-21 | Эксофарм Лтд | Способы и композиции для очистки или выделения микровезикул и экзосом |
| KR101875594B1 (ko) * | 2017-01-13 | 2018-07-06 | ㈜로제타엑소좀 | 금속을 이용한 세포밖 소포체의 분리 방법 |
| US11891664B2 (en) | 2017-05-17 | 2024-02-06 | Exosome Diagnostics, Inc. | Methods of detecting renal transplant rejection using microvesicle nucleic acid biomarkers |
| EP3660142A4 (en) * | 2017-07-26 | 2021-05-05 | Rosetta Exosome | METHOD OF ISOLATION OF EXTRACELLULAR VESICLES USING CATIONS |
| BR112020026067A2 (pt) | 2018-06-18 | 2021-03-23 | Exopharm Limited | métodos e composições para purificação ou isolamento de microvesículas e exossomas |
| WO2020077294A1 (en) * | 2018-10-12 | 2020-04-16 | Simpore, Inc. | Extracellular vesicle isolation by nanomembranes |
| US12258587B2 (en) | 2018-11-20 | 2025-03-25 | Exosome Diagnostics, Inc. | Compositions and methods for internal controls of microvesicle isolations |
| CN111254138A (zh) * | 2018-12-03 | 2020-06-09 | 北京银丰鼎诚生物工程技术有限公司 | 一种分子克隆中提高酶切产物浓缩效率的方法 |
| EP4671268A2 (en) | 2019-03-21 | 2025-12-31 | Lonza Sales AG | METHOD FOR PREPARING EXTRACELLULAR VESICLES |
| GB201906722D0 (en) * | 2019-05-13 | 2019-06-26 | Ttp Plc | A method of preparing a sample for a diagnostic assay |
| EP4031865A2 (en) | 2019-09-18 | 2022-07-27 | Exosome Diagnostics, Inc. | Compositions, methods, and kits for the isolation of extracellular vesicles |
| WO2021122846A1 (en) | 2019-12-16 | 2021-06-24 | Qiagen Gmbh | Enrichment method |
| EP4077660A1 (en) | 2019-12-16 | 2022-10-26 | QIAGEN GmbH | Method for enriching vesicular rna |
| JP2023508411A (ja) * | 2019-12-23 | 2023-03-02 | バイオプセンス オサケユキチュア | 血液試料からセルフリー核酸を抽出するための方法、自動化システムおよびカートリッジ |
| EP4137559A4 (en) * | 2020-04-15 | 2024-06-12 | H.U. Group Research Institute G.K. | METHOD FOR OBTAINING EXTRACELLULAR VESICLES |
| WO2021217173A1 (en) * | 2020-04-21 | 2021-10-28 | Siemens Healthcare Diagnostics Inc. | Clinical specimen collection swabs for molecular diagnostic applications and methods of production and use thereof |
| CA3180572A1 (en) | 2020-05-29 | 2021-12-02 | Johan Skog | Use of microvesicle signature for the diagnosis and treatment of kidney transplant rejection |
| CA3193107A1 (en) * | 2020-09-23 | 2022-03-31 | Codiak Biosciences, Inc. | Process for preparing extracellular vesicles |
| CZ35067U1 (cs) * | 2020-12-15 | 2021-05-18 | Univerzita Palackého v Olomouci | Sada pro izolaci RNA |
| JP7773765B2 (ja) * | 2020-12-18 | 2025-11-20 | 国立研究開発法人産業技術総合研究所 | 回収用キットおよび核酸含有溶液の調製方法 |
| US20220315992A1 (en) * | 2021-04-06 | 2022-10-06 | ID Match, LLC | Method for identification of urine drug testing sample |
| WO2022263500A1 (en) * | 2021-06-17 | 2022-12-22 | Qiagen Gmbh | Method for isolating non-vesicular mirna |
| EP4370712A1 (en) | 2021-07-16 | 2024-05-22 | Exosome Diagnostics, Inc. | Methods of detecting sjögren's syndrome using salivary exosomes |
| DE102021208893B3 (de) | 2021-08-13 | 2022-11-17 | Hahn-Schickard-Gesellschaft für angewandte Forschung e.V. | Isolieren von Analyten unterschiedlicher Analyt-Klassen |
| SE2100130A1 (en) * | 2021-09-01 | 2023-03-02 | Frontline Diagnostics AB | System for integrated automated isolation and analysis of biological vesicles and associated molecules |
| AU2022379949A1 (en) * | 2021-11-04 | 2024-05-02 | Mayo Foundation For Medical Education And Research | Methods and materials for treating cancer |
| AU2023221644A1 (en) | 2022-02-18 | 2024-09-12 | Bio-Techne Corporation | Use of microvesicle signatures in the identification and treatment of renal disorders |
| EP4584397A1 (en) | 2022-09-07 | 2025-07-16 | Exosome Diagnostics, Inc. | Methods of detecting sjögren's syndrome using salivary exosomes |
| WO2024185655A1 (ja) * | 2023-03-06 | 2024-09-12 | 東ソー株式会社 | 細胞外小胞精製用磁性粒子およびその製造方法、ならびに、細胞外小胞の精製方法 |
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| US20150010951A1 (en) | 2010-10-28 | 2015-01-08 | Clinical Genomics Pty. Ltd. | Method of microvesicle enrichment |
| WO2016007755A1 (en) | 2014-07-09 | 2016-01-14 | Skog Johan Karl Olov | Methods for isolating microvesicles and extracting nucleic acids from biological samples |
| JP2016502862A (ja) | 2013-01-03 | 2016-02-01 | エクソサム ダイアグノスティクス,インコーポレイティド | 微細小胞の単離方法 |
| US20160115471A1 (en) | 2014-10-24 | 2016-04-28 | Dae Hyun H. Kim | Enrichment of small nucleic acids |
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-
2017
- 2017-05-15 AU AU2017263886A patent/AU2017263886B2/en active Active
- 2017-05-15 EP EP22167997.0A patent/EP4079853A1/en active Pending
- 2017-05-15 JP JP2018559313A patent/JP7037504B2/ja active Active
- 2017-05-15 CN CN201780043334.9A patent/CN109642229A/zh active Pending
- 2017-05-15 US US16/301,176 patent/US10808240B2/en active Active
- 2017-05-15 EP EP17728304.1A patent/EP3455355B1/en active Active
- 2017-05-15 WO PCT/US2017/032719 patent/WO2017197399A1/en not_active Ceased
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2020
- 2020-10-19 US US17/074,040 patent/US20210238582A1/en active Pending
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2022
- 2022-03-04 JP JP2022033452A patent/JP7354327B2/ja active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2008510456A (ja) | 2004-07-30 | 2008-04-10 | アジェンコート バイオサイエンス コーポレーション | 多官能基コート化固相担体を用いる核酸の単離方法 |
| US20150010951A1 (en) | 2010-10-28 | 2015-01-08 | Clinical Genomics Pty. Ltd. | Method of microvesicle enrichment |
| JP2016502862A (ja) | 2013-01-03 | 2016-02-01 | エクソサム ダイアグノスティクス,インコーポレイティド | 微細小胞の単離方法 |
| WO2016007755A1 (en) | 2014-07-09 | 2016-01-14 | Skog Johan Karl Olov | Methods for isolating microvesicles and extracting nucleic acids from biological samples |
| US20160115471A1 (en) | 2014-10-24 | 2016-04-28 | Dae Hyun H. Kim | Enrichment of small nucleic acids |
Non-Patent Citations (1)
| Title |
|---|
| ENDERLE D; ET AL,DEVELOPMENT OF A ONE-STEP ISOLATION PLATFORM FOR EXOSOMAL RNA AND CIRCULATING CELL-FREE DNA FROM CANCER PLASMA SAMPLES,EUROPEAN JOURNAL OF CANCER - POSTER SESSION,2014年11月,VOL:50, NR:SUPPL.6,PAGE(S):102,http://dx.doi.org/10.1016/S0959-8049(14)70439-3 |
Also Published As
| Publication number | Publication date |
|---|---|
| WO2017197399A1 (en) | 2017-11-16 |
| US20190284548A1 (en) | 2019-09-19 |
| CN109642229A (zh) | 2019-04-16 |
| US10808240B2 (en) | 2020-10-20 |
| US20210238582A1 (en) | 2021-08-05 |
| EP3455355A1 (en) | 2019-03-20 |
| AU2017263886A1 (en) | 2018-11-22 |
| EP4079853A1 (en) | 2022-10-26 |
| JP2022084700A (ja) | 2022-06-07 |
| CA3022528A1 (en) | 2017-11-16 |
| JP2019521322A (ja) | 2019-07-25 |
| JP7354327B2 (ja) | 2023-10-02 |
| EP3455355B1 (en) | 2022-04-13 |
| AU2017263886B2 (en) | 2023-11-09 |
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