JP7016215B2 - 配列決定のために核酸を調製する方法 - Google Patents

配列決定のために核酸を調製する方法 Download PDF

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JP7016215B2
JP7016215B2 JP2016549249A JP2016549249A JP7016215B2 JP 7016215 B2 JP7016215 B2 JP 7016215B2 JP 2016549249 A JP2016549249 A JP 2016549249A JP 2016549249 A JP2016549249 A JP 2016549249A JP 7016215 B2 JP7016215 B2 JP 7016215B2
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primers
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アンソニー ジョン イアフレート
ロン フィー レー
ツォンリ ツェン
ジェイソン マイヤーズ
ジョシュア スタール
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ザ ジェネラル ホスピタル コーポレイション
アーチャーディーエックス インコーポレイテッド
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    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6809Methods for determination or identification of nucleic acids involving differential detection

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JP2016549249A 2014-01-27 2015-01-26 配列決定のために核酸を調製する方法 Active JP7016215B2 (ja)

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US201461931959P 2014-01-27 2014-01-27
US61/931,959 2014-01-27
PCT/US2015/012891 WO2015112974A1 (en) 2014-01-27 2015-01-26 Methods of preparing nucleic acids for sequencing

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US (4) US10450597B2 (OSRAM)
EP (2) EP3099820A4 (OSRAM)
JP (2) JP7016215B2 (OSRAM)
CN (1) CN106661612A (OSRAM)
AU (2) AU2015209079B2 (OSRAM)
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Families Citing this family (81)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9424392B2 (en) 2005-11-26 2016-08-23 Natera, Inc. System and method for cleaning noisy genetic data from target individuals using genetic data from genetically related individuals
US8835358B2 (en) 2009-12-15 2014-09-16 Cellular Research, Inc. Digital counting of individual molecules by stochastic attachment of diverse labels
US11408031B2 (en) 2010-05-18 2022-08-09 Natera, Inc. Methods for non-invasive prenatal paternity testing
US11332785B2 (en) 2010-05-18 2022-05-17 Natera, Inc. Methods for non-invasive prenatal ploidy calling
US11322224B2 (en) 2010-05-18 2022-05-03 Natera, Inc. Methods for non-invasive prenatal ploidy calling
US12152275B2 (en) 2010-05-18 2024-11-26 Natera, Inc. Methods for non-invasive prenatal ploidy calling
US12221653B2 (en) 2010-05-18 2025-02-11 Natera, Inc. Methods for simultaneous amplification of target loci
US11326208B2 (en) 2010-05-18 2022-05-10 Natera, Inc. Methods for nested PCR amplification of cell-free DNA
US11332793B2 (en) 2010-05-18 2022-05-17 Natera, Inc. Methods for simultaneous amplification of target loci
US8825412B2 (en) 2010-05-18 2014-09-02 Natera, Inc. Methods for non-invasive prenatal ploidy calling
US10316362B2 (en) 2010-05-18 2019-06-11 Natera, Inc. Methods for simultaneous amplification of target loci
US20190010543A1 (en) 2010-05-18 2019-01-10 Natera, Inc. Methods for simultaneous amplification of target loci
US9677118B2 (en) 2014-04-21 2017-06-13 Natera, Inc. Methods for simultaneous amplification of target loci
US11339429B2 (en) 2010-05-18 2022-05-24 Natera, Inc. Methods for non-invasive prenatal ploidy calling
US11939634B2 (en) 2010-05-18 2024-03-26 Natera, Inc. Methods for simultaneous amplification of target loci
CA2824387C (en) 2011-02-09 2019-09-24 Natera, Inc. Methods for non-invasive prenatal ploidy calling
GB2513024B (en) 2012-02-27 2016-08-31 Cellular Res Inc A clonal amplification method
US20140100126A1 (en) 2012-08-17 2014-04-10 Natera, Inc. Method for Non-Invasive Prenatal Testing Using Parental Mosaicism Data
GB2546833B (en) 2013-08-28 2018-04-18 Cellular Res Inc Microwell for single cell analysis comprising single cell and single bead oligonucleotide capture labels
CN106029962A (zh) 2014-02-11 2016-10-12 豪夫迈·罗氏有限公司 靶向测序和uid过滤
US12492429B2 (en) 2014-04-21 2025-12-09 Natera, Inc. Detecting mutations and ploidy in chromosomal segments
EP3957749A1 (en) 2014-04-21 2022-02-23 Natera, Inc. Detecting tumour specific mutations in biopsies with whole exome sequencing and in cell-free samples
US20180173846A1 (en) 2014-06-05 2018-06-21 Natera, Inc. Systems and Methods for Detection of Aneuploidy
CN107208157B (zh) * 2015-02-27 2022-04-05 贝克顿迪金森公司 用于条形编码核酸以用于测序的方法和组合物
EP3262192B1 (en) 2015-02-27 2020-09-16 Becton, Dickinson and Company Spatially addressable molecular barcoding
WO2016160844A2 (en) 2015-03-30 2016-10-06 Cellular Research, Inc. Methods and compositions for combinatorial barcoding
EP3286326B1 (en) 2015-04-23 2025-01-22 Becton, Dickinson and Company Method for whole transcriptome amplification
US11479812B2 (en) 2015-05-11 2022-10-25 Natera, Inc. Methods and compositions for determining ploidy
JP6847496B2 (ja) 2015-08-06 2021-03-24 エフ.ホフマン−ラ ロシュ アーゲーF. Hoffmann−La Roche Aktiengesellschaft 単一プローブプライマー伸長による標的濃縮
EP3347465B1 (en) 2015-09-11 2019-06-26 Cellular Research, Inc. Methods and compositions for nucleic acid library normalization
ES2893000T3 (es) * 2015-12-01 2022-02-07 Seracare Life Sciences Inc Materiales de referencia celulares multiplex
JP7280044B2 (ja) 2016-04-15 2023-05-23 ナテラ, インコーポレイテッド 肺癌の検出方法
US10301677B2 (en) 2016-05-25 2019-05-28 Cellular Research, Inc. Normalization of nucleic acid libraries
US10640763B2 (en) 2016-05-31 2020-05-05 Cellular Research, Inc. Molecular indexing of internal sequences
US10202641B2 (en) 2016-05-31 2019-02-12 Cellular Research, Inc. Error correction in amplification of samples
EP3464629B1 (en) 2016-06-01 2021-09-08 F. Hoffmann-La Roche AG Immuno-pete
US10907204B2 (en) * 2016-07-12 2021-02-02 Roche Sequencing Solutions, Inc. Primer extension target enrichment
US10683531B2 (en) 2016-09-15 2020-06-16 ArcherDX, Inc. Methods of nucleic acid sample preparation for analysis of cell-free DNA
US20220282305A1 (en) * 2016-09-15 2022-09-08 Archerdx, Llc Methods of nucleic acid sample preparation
CA3037185A1 (en) 2016-09-15 2018-03-22 ArcherDX, Inc. Methods of nucleic acid sample preparation
CN109791157B (zh) 2016-09-26 2022-06-07 贝克顿迪金森公司 使用具有条形码化的寡核苷酸序列的试剂测量蛋白质表达
WO2018067517A1 (en) 2016-10-04 2018-04-12 Natera, Inc. Methods for characterizing copy number variation using proximity-litigation sequencing
WO2018081113A1 (en) 2016-10-24 2018-05-03 Sawaya Sterling Concealing information present within nucleic acids
CA3042391A1 (en) * 2016-11-02 2018-05-11 ArcherDX, Inc. Methods of nucleic acid sample preparation for immune repertoire sequencing
GB201618485D0 (en) 2016-11-02 2016-12-14 Ucl Business Plc Method of detecting tumour recurrence
US10011870B2 (en) 2016-12-07 2018-07-03 Natera, Inc. Compositions and methods for identifying nucleic acid molecules
CN106755451A (zh) * 2017-01-05 2017-05-31 苏州艾达康医疗科技有限公司 核酸制备及分析
WO2018144240A1 (en) 2017-02-01 2018-08-09 Cellular Research, Inc. Selective amplification using blocking oligonucleotides
US12297491B2 (en) 2017-03-08 2025-05-13 Roche Sequencing Solutions, Inc. Primer extension target enrichment and improvements thereto including simultaneous enrichment of DNA and RNA
JP6970205B2 (ja) 2017-03-08 2021-11-24 エフ.ホフマン−ラ ロシュ アーゲーF. Hoffmann−La Roche Aktiengesellschaft Dnaおよびrnaの同時濃縮を含むプライマー伸長標的濃縮およびそれに対する向上
AU2018281745B2 (en) 2017-06-05 2022-05-19 Becton, Dickinson And Company Sample indexing for single cells
US11505826B2 (en) * 2017-07-12 2022-11-22 Agilent Technologies, Inc. Sequencing method for genomic rearrangement detection
JP6998404B2 (ja) * 2017-08-01 2022-02-04 深▲セン▼恒特基因有限公司 標的ヌクレオチド配列の富化及び決定方法
WO2019090482A1 (zh) * 2017-11-07 2019-05-16 北京优乐复生科技有限责任公司 一种第二代高通量测序文库构建方法
WO2019118926A1 (en) 2017-12-14 2019-06-20 Tai Diagnostics, Inc. Assessing graft suitability for transplantation
EP4269582A3 (en) * 2017-12-21 2024-01-24 F. Hoffmann-La Roche AG Target enrichment by unidirectional dual probe primer extension
CN110241209B (zh) * 2018-03-09 2022-11-29 浙江品级基因科技有限公司 一种引物、试剂盒及用途
US12024738B2 (en) 2018-04-14 2024-07-02 Natera, Inc. Methods for cancer detection and monitoring
ES2945191T3 (es) 2018-05-03 2023-06-29 Becton Dickinson Co Análisis de muestras multiómicas de alto rendimiento
EP3788170B1 (en) 2018-05-03 2025-01-01 Becton, Dickinson and Company Molecular barcoding on opposite transcript ends
US12234509B2 (en) 2018-07-03 2025-02-25 Natera, Inc. Methods for detection of donor-derived cell-free DNA
EP4471156A3 (en) 2018-10-01 2025-02-26 Becton, Dickinson and Company Determining 5' transcript sequences
WO2020097315A1 (en) 2018-11-08 2020-05-14 Cellular Research, Inc. Whole transcriptome analysis of single cells using random priming
US20220010306A1 (en) 2018-11-30 2022-01-13 Geneinfosec, Inc. A method for generating random oligonucleotides and determining their sequence
CN113195717A (zh) 2018-12-13 2021-07-30 贝克顿迪金森公司 单细胞全转录组分析中的选择性延伸
WO2020154247A1 (en) 2019-01-23 2020-07-30 Cellular Research, Inc. Oligonucleotides associated with antibodies
EP3924506A1 (en) 2019-02-14 2021-12-22 Becton Dickinson and Company Hybrid targeted and whole transcriptome amplification
WO2020247263A1 (en) 2019-06-06 2020-12-10 Natera, Inc. Methods for detecting immune cell dna and monitoring immune system
CN114051534B (zh) 2019-07-22 2025-02-21 贝克顿迪金森公司 单细胞染色质免疫沉淀测序测定
EP4407041A3 (en) 2019-11-08 2024-09-25 Becton Dickinson and Company Using random priming to obtain full-length v(d)j information for immune repertoire sequencing
US11649497B2 (en) 2020-01-13 2023-05-16 Becton, Dickinson And Company Methods and compositions for quantitation of proteins and RNA
EP4471155A3 (en) 2020-01-29 2024-12-18 Becton, Dickinson and Company Barcoded wells for spatial mapping of single cells through sequencing
CN115151810A (zh) 2020-02-25 2022-10-04 贝克顿迪金森公司 实现使用单细胞样品作为单色补偿对照的双特异性探针
CN115605614A (zh) 2020-05-14 2023-01-13 贝克顿迪金森公司(Us) 用于免疫组库谱分析的引物
US12157913B2 (en) 2020-06-02 2024-12-03 Becton, Dickinson And Company Oligonucleotides and beads for 5 prime gene expression assay
US11932901B2 (en) 2020-07-13 2024-03-19 Becton, Dickinson And Company Target enrichment using nucleic acid probes for scRNAseq
US12391940B2 (en) 2020-07-31 2025-08-19 Becton, Dickinson And Company Single cell assay for transposase-accessible chromatin
JP2023547732A (ja) * 2020-11-03 2023-11-13 エイシーティ・ジェノミックス(アイピー)リミテッド 遺伝子改変を検出するための標的化シークエンシング方法およびそのキット
US11739443B2 (en) 2020-11-20 2023-08-29 Becton, Dickinson And Company Profiling of highly expressed and lowly expressed proteins
US12392771B2 (en) 2020-12-15 2025-08-19 Becton, Dickinson And Company Single cell secretome analysis
EP4448793A4 (en) * 2021-12-14 2025-12-17 Univ Arizona State METHOD FOR COMBINATION OF IN-SITU SINGLE-CELL DNA AND RNA SEQUENCEMENT

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20090203085A1 (en) 2008-02-12 2009-08-13 Nurith Kurn Isothermal Nucleic Acid Amplification Methods and Compositions
WO2013169339A1 (en) 2012-05-10 2013-11-14 The General Hospital Corporation Methods for determining a nucleotide sequence

Family Cites Families (106)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4868104A (en) 1985-09-06 1989-09-19 Syntex (U.S.A.) Inc. Homogeneous assay for specific polynucleotides
US6027913A (en) 1988-01-28 2000-02-22 Sommer; Steven S. Nucleic acid amplification with direct sequencing
GB8818020D0 (en) 1988-07-28 1988-09-01 Ici Plc Method for amplification of nucleotide sequences
US6087101A (en) 1990-05-18 2000-07-11 Gruelich; Karl Otto Optical characterization of nucleic acids and oligonucleotides
US5846719A (en) 1994-10-13 1998-12-08 Lynx Therapeutics, Inc. Oligonucleotide tags for sorting and identification
US5565340A (en) 1995-01-27 1996-10-15 Clontech Laboratories, Inc. Method for suppressing DNA fragment amplification during PCR
JP2001517925A (ja) 1995-12-22 2001-10-09 ベーリングウエルケ、アクティエンゲゼルシャフト 核酸の均質増幅および検出
US6013439A (en) 1995-12-22 2000-01-11 Dade Behring Marburg Gmbh Detection of differences in nucleic acids
US5827658A (en) 1996-08-09 1998-10-27 The United States Of America As Reprsented By The Department Of Health And Human Services Isolation of amplified genes via cDNA subtractive hybridization
US6482590B1 (en) 1996-12-20 2002-11-19 Aventis Behring Gmbh Method for polynucleotide amplification
US6365346B1 (en) 1998-02-18 2002-04-02 Dade Behring Inc. Quantitative determination of nucleic acid amplification products
US6235502B1 (en) 1998-09-18 2001-05-22 Molecular Staging Inc. Methods for selectively isolating DNA using rolling circle amplification
US6200757B1 (en) 1999-01-19 2001-03-13 Dade Behring Inc. Method for controlling the extension of an oligonucleotide
WO2000043543A1 (en) 1999-01-19 2000-07-27 Dade Behring Inc. Detection of differences between polynucleotides
WO2000070095A2 (en) 1999-05-17 2000-11-23 Dade Behring Inc. Homogeneous isothermal amplification and detection of nucleic acids using a template switch oligonucleotide
US6818395B1 (en) 1999-06-28 2004-11-16 California Institute Of Technology Methods and apparatus for analyzing polynucleotide sequences
US6232104B1 (en) 1999-08-17 2001-05-15 Dade Behring Inc. Detection of differences in nucleic acids by inhibition of spontaneous DNA branch migration
AU783873B2 (en) 1999-09-13 2005-12-15 Nugen Technologies, Inc. Methods and compositions for linear isothermal amplification of polynucleotide sequences
US7244559B2 (en) 1999-09-16 2007-07-17 454 Life Sciences Corporation Method of sequencing a nucleic acid
EP1218543A2 (en) 1999-09-29 2002-07-03 Solexa Ltd. Polynucleotide sequencing
US6309836B1 (en) 1999-10-05 2001-10-30 Marek Kwiatkowski Compounds for protecting hydroxyls and methods for their use
WO2001083696A2 (en) 2000-04-28 2001-11-08 Digital Gene Tech Inc Methods for rapid isolation and sequence determination of gene-specific sequences
EP1356094B1 (en) 2000-06-26 2010-01-13 Nugen Technologies, Inc. Methods and compositions for transcription-based nucleic acid amplification
EP1176212A1 (en) 2000-07-24 2002-01-30 Centre National de Genotype Method for haplotyping by mass spectrometry
JP2004537257A (ja) 2000-10-06 2004-12-16 ニューゲン テクノロジーズ, インコーポレイテッド 核酸配列の検出および/または定量のための、方法およびプローブ
JP2002153271A (ja) 2000-11-17 2002-05-28 Jeol Ltd Dnaあるいはrnaの塩基配列決定方法およびdnaシーケンサー
CA2430329A1 (en) 2000-12-13 2002-06-20 Nugen Technologies, Inc. Methods and compositions for generation of multiple copies of nucleic acid sequences and methods of detection thereof
AU2005203617A1 (en) * 2001-03-09 2005-09-01 Nugen Technologies, Inc Methods and compositions for amplification of RNA sequences
NZ523167A (en) 2001-03-09 2006-08-31 Nugen Technologies Inc Methods, compositions and kits for amplifying RNA sequences of interest which employ an RNA/DNA composite primer
US6632611B2 (en) 2001-07-20 2003-10-14 Affymetrix, Inc. Method of target enrichment and amplification
US20030104432A1 (en) 2001-07-27 2003-06-05 The Regents Of The University Of California Methods of amplifying sense strand RNA
ATE405676T1 (de) 2002-03-11 2008-09-15 Nugen Technologies Inc Verfahren zur erzeugung doppelsträngiger dna mit einem 3'-einzelstranganteil und verwendungen dieser komplexe zur rekombination
JP2005521409A (ja) 2002-03-29 2005-07-21 ニューゲン テクノロジーズ, インコーポレイテッド 単一プライマー等温核酸増幅−増強型被分析物検出および定量
WO2004011665A2 (en) 2002-05-17 2004-02-05 Nugen Technologies, Inc. Methods for fragmentation, labeling and immobilization of nucleic acids
WO2003100019A2 (en) 2002-05-24 2003-12-04 Invitrogen Corporation Nested pcr employing degradable primers
US7459273B2 (en) 2002-10-04 2008-12-02 Affymetrix, Inc. Methods for genotyping selected polymorphism
EP1594950A4 (en) 2003-01-29 2007-07-25 454 Corp PRODUCT FOR THE PREPARATION OF SIMPLE STRANDED DNA BANKS
DK1454992T3 (da) 2003-03-07 2006-10-02 Ist Naz Stud Cura Dei Tumori Anaplastisk lymfomakinaseassay, reagenser og sammensætninger deraf
JP2006523465A (ja) 2003-04-14 2006-10-19 ニューゲン テクノロジーズ, インコーポレイテッド ランダムにプライミングされる複合プライマーを用いる大規模増幅
US7226720B2 (en) 2003-09-08 2007-06-05 General Electric Company Limited play data storage media and method for limiting access to data thereon
ATE461292T1 (de) 2003-09-10 2010-04-15 Althea Technologies Inc Erstellung von expressionsprofilen unter verwendung von mikroarrays
WO2005045073A1 (en) * 2003-11-10 2005-05-19 Seegene, Inc. Method for amplifying unknown dna sequence adjacent to known sequence
US7169560B2 (en) 2003-11-12 2007-01-30 Helicos Biosciences Corporation Short cycle methods for sequencing polynucleotides
ES2527118T3 (es) 2003-12-19 2015-01-20 Plexxikon Inc. Compuestos y procedimientos de desarrollo de moduladores de Ret
US20050208538A1 (en) 2003-12-29 2005-09-22 Nurith Kurn Methods for analysis of nucleic acid methylation status and methods for fragmentation, labeling and immobilization of nucleic acids
JP3972106B2 (ja) 2004-03-03 2007-09-05 大学共同利用機関法人情報・システム研究機構 ゲノムライブラリー作製方法、および同方法により作製されたゲノムライブラリー
GB0421525D0 (en) 2004-09-28 2004-10-27 Novartis Ag Inhibitors of protein kineses
US20070070349A1 (en) 2005-09-23 2007-03-29 Helicos Biosciences Corporation Optical train and method for TIRF single molecule detection and analysis
US7220549B2 (en) 2004-12-30 2007-05-22 Helicos Biosciences Corporation Stabilizing a nucleic acid for nucleic acid sequencing
WO2007030759A2 (en) 2005-09-07 2007-03-15 Nugen Technologies, Inc. Improved nucleic acid amplification procedure
GB0522310D0 (en) 2005-11-01 2005-12-07 Solexa Ltd Methods of preparing libraries of template polynucleotides
EP1957667A1 (en) 2005-11-15 2008-08-20 Solexa Ltd. Method of target enrichment
US7282337B1 (en) 2006-04-14 2007-10-16 Helicos Biosciences Corporation Methods for increasing accuracy of nucleic acid sequencing
ES2400805T3 (es) * 2006-05-04 2013-04-12 Seegene, Inc. Método para amplificar una secuencia de ADN desconocida adyacente a una secuencia conocida
CA2652052A1 (en) 2006-05-16 2007-11-29 Nugen Technologies, Inc. Nucleic acid separation and purification method based on reversible charge interactions
US20100022403A1 (en) 2006-06-30 2010-01-28 Nurith Kurn Methods for fragmentation and labeling of nucleic acids
AU2007334393A1 (en) 2006-12-14 2008-06-26 Life Technologies Corporation Methods and apparatus for measuring analytes using large scale FET arrays
EP2121983A2 (en) 2007-02-02 2009-11-25 Illumina Cambridge Limited Methods for indexing samples and sequencing multiple nucleotide templates
US8716190B2 (en) 2007-09-14 2014-05-06 Affymetrix, Inc. Amplification and analysis of selected targets on solid supports
WO2009052547A1 (en) 2007-10-22 2009-04-30 Monoquant Pty Ltd A method of dna amplification
WO2009102896A2 (en) 2008-02-12 2009-08-20 Nugen Technologies, Inc. Isothermal nucleic acid amplification methods and compositions
GB2470672B (en) 2008-03-21 2012-09-12 Nugen Technologies Inc Methods of RNA amplification in the presence of DNA
WO2009133466A2 (en) 2008-04-30 2009-11-05 Population Genetics Technologies Ltd. Asymmetric adapter library construction
US20110172105A1 (en) 2008-06-04 2011-07-14 Salk Institute For Biological Studies GREPSEQ: An Almost Inexhaustible, Cost-Effective, High-Throughput Protocol for the Generation of Selector Sequences
WO2010006432A1 (en) 2008-07-14 2010-01-21 Queen's University At Kingston Pharmaceutical compositions comprising ret inhibitors and methods for the treatment of cancer
WO2010077288A2 (en) 2008-12-09 2010-07-08 The Salk Institute For Biological Studies Methods for identifying differences in alternative splicing between two rna samples
WO2010083046A2 (en) 2009-01-15 2010-07-22 The Salk Institute For Biological Studies Methods for using next generation sequencing to identify 5-methyl cytosines in the genome
US20100286143A1 (en) 2009-04-24 2010-11-11 Dora Dias-Santagata Methods and materials for genetic analysis of tumors
WO2011019964A1 (en) 2009-08-12 2011-02-17 Nugen Technologies, Inc. Methods, compositions, and kits for generating nucleic acid products substantially free of template nucleic acid
EP2475777A4 (en) 2009-09-11 2013-03-06 Nugen Technologies Inc COMPOSITIONS AND METHODS FOR COMPLETE TRANSCRIPTOMAL ANALYSIS
US20110105364A1 (en) 2009-11-02 2011-05-05 Nugen Technologies, Inc. Compositions and methods for targeted nucleic acid sequence selection and amplification
WO2011056866A2 (en) 2009-11-05 2011-05-12 Epicentre Technologies Corporation Methods and kits for 3'-end-tagging of rna
JP5795341B2 (ja) * 2010-03-08 2015-10-14 デイナ ファーバー キャンサー インスティチュート,インコーポレイテッド 参照ブロック配列によるfullCOLD−PCR濃縮
US20110269647A1 (en) 2010-04-28 2011-11-03 Medical Research Council Method
US8825412B2 (en) 2010-05-18 2014-09-02 Natera, Inc. Methods for non-invasive prenatal ploidy calling
CN103119439A (zh) 2010-06-08 2013-05-22 纽亘技术公司 用于多重测序的方法和组合物
US20120003657A1 (en) 2010-07-02 2012-01-05 Samuel Myllykangas Targeted sequencing library preparation by genomic dna circularization
EP3115468B1 (en) 2010-09-21 2018-07-25 Agilent Technologies, Inc. Increasing confidence of allele calls with molecular counting
WO2012040387A1 (en) 2010-09-24 2012-03-29 The Board Of Trustees Of The Leland Stanford Junior University Direct capture, amplification and sequencing of target dna using immobilized primers
US20120100549A1 (en) * 2010-10-01 2012-04-26 Ibis Biosciences, Inc. Targeted genome amplification methods
EP2630263B2 (en) 2010-10-22 2021-11-10 Cold Spring Harbor Laboratory Varietal counting of nucleic acids for obtaining genomic copy number information
WO2012064739A2 (en) * 2010-11-08 2012-05-18 The Trustees Of Columbia University In The City Of New York Microbial enrichment primers
CA2821906C (en) 2010-12-22 2020-08-25 Natera, Inc. Methods for non-invasive prenatal paternity testing
WO2012103154A1 (en) 2011-01-24 2012-08-02 Nugen Technologies, Inc. Stem-loop composite rna-dna adaptor-primers: compositions and methods for library generation, amplification and other downstream manipulations
CA2824387C (en) 2011-02-09 2019-09-24 Natera, Inc. Methods for non-invasive prenatal ploidy calling
US9150852B2 (en) 2011-02-18 2015-10-06 Raindance Technologies, Inc. Compositions and methods for molecular labeling
WO2012131616A1 (en) * 2011-03-30 2012-10-04 Chromous Biotech Pvt. Ltd. Primer set, kit and methods thereof
AU2012242847B2 (en) 2011-04-15 2017-01-19 The Johns Hopkins University Safe sequencing system
EP2710172B1 (en) * 2011-05-20 2017-03-29 Fluidigm Corporation Nucleic acid encoding reactions
WO2012166425A2 (en) 2011-05-27 2012-12-06 President And Fellows Of Harvard College Methods of amplifying whole genome of a single cell
US8841071B2 (en) 2011-06-02 2014-09-23 Raindance Technologies, Inc. Sample multiplexing
GB2497838A (en) 2011-10-19 2013-06-26 Nugen Technologies Inc Compositions and methods for directional nucleic acid amplification and sequencing
US20130123117A1 (en) 2011-11-16 2013-05-16 The Board Of Trustees Of The Leland Stanford Junior University Capture probe and assay for analysis of fragmented nucleic acids
CA2862552A1 (en) 2012-01-26 2013-08-01 Nugen Technologies, Inc. Compositions and methods for targeted nucleic acid sequence enrichment and high efficiency library generation
US20150045258A1 (en) 2012-02-14 2015-02-12 Gnubio, Inc. Cascaded addition of target specific universal adapters to nucleic acids
ES2855130T3 (es) 2012-02-17 2021-09-23 Hutchinson Fred Cancer Res Composiciones y métodos para identificar mutaciones de manera precisa
JP6181751B2 (ja) 2012-06-18 2017-08-16 ニューゲン テクノロジーズ, インコーポレイテッド 望まれない核酸配列のネガティブ選択のための組成物および方法
US20150011396A1 (en) 2012-07-09 2015-01-08 Benjamin G. Schroeder Methods for creating directional bisulfite-converted nucleic acid libraries for next generation sequencing
AU2013325107C1 (en) 2012-09-25 2019-02-28 Agriculture Victoria Services Pty Ltd Method of producing a normalised nucleic acid library using solid state capture material
US20140274738A1 (en) 2013-03-15 2014-09-18 Nugen Technologies, Inc. Sequential sequencing
US20140274729A1 (en) 2013-03-15 2014-09-18 Nugen Technologies, Inc. Methods, compositions and kits for generation of stranded rna or dna libraries
EP3068883B1 (en) 2013-11-13 2020-04-29 Nugen Technologies, Inc. Compositions and methods for identification of a duplicate sequencing read
US9689027B2 (en) 2013-11-15 2017-06-27 The Board Of Trustees Of The Leland Stanford Junior University High efficiency multiplexed nucleic acid capture in a structured microenvironment
US10683531B2 (en) 2016-09-15 2020-06-16 ArcherDX, Inc. Methods of nucleic acid sample preparation for analysis of cell-free DNA
CA3037185A1 (en) 2016-09-15 2018-03-22 ArcherDX, Inc. Methods of nucleic acid sample preparation
CA3042391A1 (en) 2016-11-02 2018-05-11 ArcherDX, Inc. Methods of nucleic acid sample preparation for immune repertoire sequencing

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20090203085A1 (en) 2008-02-12 2009-08-13 Nurith Kurn Isothermal Nucleic Acid Amplification Methods and Compositions
WO2013169339A1 (en) 2012-05-10 2013-11-14 The General Hospital Corporation Methods for determining a nucleotide sequence

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
BMC Biotechnol.,2011年,Vol.11,109(p.1-12)
Virology,2004年,Vol.330,p.186-195

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