JP7008016B2 - 増幅産生物の富化のための方法および組成物 - Google Patents

増幅産生物の富化のための方法および組成物 Download PDF

Info

Publication number
JP7008016B2
JP7008016B2 JP2018517602A JP2018517602A JP7008016B2 JP 7008016 B2 JP7008016 B2 JP 7008016B2 JP 2018517602 A JP2018517602 A JP 2018517602A JP 2018517602 A JP2018517602 A JP 2018517602A JP 7008016 B2 JP7008016 B2 JP 7008016B2
Authority
JP
Japan
Prior art keywords
sequence
primer
target polynucleotide
polynucleotide
amplification
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
JP2018517602A
Other languages
English (en)
Japanese (ja)
Other versions
JP2018537955A (ja
JP2018537955A5 (https=
Inventor
リ ウェン，
シェンロン リン，
リン ファン タン，
Original Assignee
アキュラジェン ホールディングス リミテッド
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by アキュラジェン ホールディングス リミテッド filed Critical アキュラジェン ホールディングス リミテッド
Publication of JP2018537955A publication Critical patent/JP2018537955A/ja
Publication of JP2018537955A5 publication Critical patent/JP2018537955A5/ja
Priority to JP2022001627A priority Critical patent/JP2022050563A/ja
Application granted granted Critical
Publication of JP7008016B2 publication Critical patent/JP7008016B2/ja
Priority to JP2024060374A priority patent/JP2024099542A/ja
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Images

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6858—Allele-specific amplification
    • G—PHYSICS
    • G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
    • G16B25/00—ICT specially adapted for hybridisation; ICT specially adapted for gene or protein expression
    • G—PHYSICS
    • G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
    • G16B25/00—ICT specially adapted for hybridisation; ICT specially adapted for gene or protein expression
    • G16B25/20—Polymerase chain reaction [PCR]; Primer or probe design; Probe optimisation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/155—Modifications characterised by incorporating/generating a new priming site
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/30—Oligonucleotides characterised by their secondary structure
    • C12Q2525/301—Hairpin oligonucleotides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/30—Oligonucleotides characterised by their secondary structure
    • C12Q2525/307—Circular oligonucleotides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2531/00—Reactions of nucleic acids characterised by
    • C12Q2531/10—Reactions of nucleic acids characterised by the purpose being amplify/increase the copy number of target nucleic acid
    • C12Q2531/125—Rolling circle
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2537/00—Reactions characterised by the reaction format or use of a specific feature
    • C12Q2537/10—Reactions characterised by the reaction format or use of a specific feature the purpose or use of
    • C12Q2537/16—Assays for determining copy number or wherein the copy number is of special importance
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/156—Polymorphic or mutational markers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/16—Primer sets for multiplex assays

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Physics & Mathematics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Analytical Chemistry (AREA)
  • Evolutionary Biology (AREA)
  • Medical Informatics (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Theoretical Computer Science (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Macromonomer-Based Addition Polymer (AREA)
JP2018517602A 2015-10-09 2016-10-07 増幅産生物の富化のための方法および組成物 Active JP7008016B2 (ja)

Priority Applications (2)

Application Number Priority Date Filing Date Title
JP2022001627A JP2022050563A (ja) 2015-10-09 2022-01-07 増幅産生物の富化のための方法および組成物
JP2024060374A JP2024099542A (ja) 2015-10-09 2024-04-03 増幅産生物の富化のための方法および組成物

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201562239690P 2015-10-09 2015-10-09
US62/239,690 2015-10-09
PCT/US2016/056126 WO2017062863A1 (en) 2015-10-09 2016-10-07 Methods and compositions for enrichment of amplification products

Related Child Applications (1)

Application Number Title Priority Date Filing Date
JP2022001627A Division JP2022050563A (ja) 2015-10-09 2022-01-07 増幅産生物の富化のための方法および組成物

Publications (3)

Publication Number Publication Date
JP2018537955A JP2018537955A (ja) 2018-12-27
JP2018537955A5 JP2018537955A5 (https=) 2019-11-14
JP7008016B2 true JP7008016B2 (ja) 2022-01-25

Family

ID=58488563

Family Applications (3)

Application Number Title Priority Date Filing Date
JP2018517602A Active JP7008016B2 (ja) 2015-10-09 2016-10-07 増幅産生物の富化のための方法および組成物
JP2022001627A Pending JP2022050563A (ja) 2015-10-09 2022-01-07 増幅産生物の富化のための方法および組成物
JP2024060374A Pending JP2024099542A (ja) 2015-10-09 2024-04-03 増幅産生物の富化のための方法および組成物

Family Applications After (2)

Application Number Title Priority Date Filing Date
JP2022001627A Pending JP2022050563A (ja) 2015-10-09 2022-01-07 増幅産生物の富化のための方法および組成物
JP2024060374A Pending JP2024099542A (ja) 2015-10-09 2024-04-03 増幅産生物の富化のための方法および組成物

Country Status (11)

Country Link
US (3) US10752942B2 (https=)
EP (1) EP3359693B1 (https=)
JP (3) JP7008016B2 (https=)
KR (1) KR20180055905A (https=)
CN (2) CN114807323A (https=)
AU (2) AU2016334233B2 (https=)
HK (1) HK1259101A1 (https=)
IL (2) IL258236B (https=)
MX (2) MX2018004284A (https=)
SG (1) SG11201802864RA (https=)
WO (1) WO2017062863A1 (https=)

Families Citing this family (26)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US10767222B2 (en) 2013-12-11 2020-09-08 Accuragen Holdings Limited Compositions and methods for detecting rare sequence variants
WO2017062863A1 (en) 2015-10-09 2017-04-13 Accuragen Holdings Limited Methods and compositions for enrichment of amplification products
US11286519B2 (en) 2013-12-11 2022-03-29 Accuragen Holdings Limited Methods and compositions for enrichment of amplification products
US11859246B2 (en) 2013-12-11 2024-01-02 Accuragen Holdings Limited Methods and compositions for enrichment of amplification products
WO2017096322A1 (en) * 2015-12-03 2017-06-08 Accuragen Holdings Limited Methods and compositions for forming ligation products
CN109511265B (zh) 2016-05-16 2023-07-14 安可济控股有限公司 通过链鉴定改进测序的方法
US10144962B2 (en) 2016-06-30 2018-12-04 Grail, Inc. Differential tagging of RNA for preparation of a cell-free DNA/RNA sequencing library
CN109844133A (zh) 2016-08-15 2019-06-04 安可济控股有限公司 检测罕见序列变体的组合物和方法
CN107760763B (zh) * 2017-11-20 2021-05-11 东南大学 一种用于茎环引物-滚环扩增反应的茎环引物以及茎环引物-滚环扩增的应用
CN109971825B (zh) * 2017-12-28 2020-11-10 南京金斯瑞生物科技有限公司 快速制备桑格测序模板的方法
US11203782B2 (en) * 2018-03-29 2021-12-21 Accuragen Holdings Limited Compositions and methods comprising asymmetric barcoding
WO2019241290A1 (en) 2018-06-12 2019-12-19 Accuragen Holdings Limited Methods and compositions for forming ligation products
EP3650558A1 (en) * 2018-11-07 2020-05-13 Siemens Healthcare GmbH Liquid sample workflow for nanopore sequencing
CN110820051B (zh) * 2018-12-28 2023-04-28 广州表观生物科技有限公司 一种高灵敏度融合基因检测方法及其应用
KR102102107B1 (ko) * 2019-05-30 2020-04-20 단국대학교 천안캠퍼스 산학협력단 이동성 유전인자 SINE 선택적 발굴을 위한 HiSeq 시퀀서 기반의 DNA 라이브러리 제작 방법
EP3990548A4 (en) * 2019-06-25 2023-07-26 AccuraGen Holdings Limited DISEASE DETECTION METHODS AND SYSTEMS
GB201911421D0 (en) * 2019-08-09 2019-09-25 Dnae Diagnostics Ltd Methods of multiplexed isothermal amplification of nucleic acid sequences
EP4028586A4 (en) * 2019-09-13 2023-10-04 University Health Network Detection of circulating tumor dna using double stranded hybrid capture
WO2021101835A1 (en) * 2019-11-19 2021-05-27 Xiao Bing Wang Method for identifying gene fusions by circle cdna amplification
EP4077735A4 (en) * 2019-12-20 2024-01-24 AccuraGen Holdings Limited METHOD AND SYSTEMS FOR DETECTING DISEASES
KR102468839B1 (ko) 2020-06-03 2022-11-18 주식회사 제노헬릭스 Rna 검출 방법
CN116075520A (zh) * 2020-06-26 2023-05-05 威廉马歇莱思大学 扩增子全面富集
KR20230091116A (ko) * 2020-10-21 2023-06-22 일루미나, 인코포레이티드 다수의 삽입체를 포함하는 시퀀싱 주형, 및 시퀀싱 처리량을 개선하기 위한 조성물 및 방법
WO2022103750A1 (en) * 2020-11-11 2022-05-19 Accuragen Holdings Limited Methods and systems for sample normalization
CN113549674B (zh) * 2021-04-22 2023-11-03 福建和瑞基因科技有限公司 一种检测样本中目标序列整合和突变的方法及其引物的设计方法和试剂盒
CN113832147B (zh) * 2021-09-08 2024-06-14 华南农业大学 一种高效的大片段dna合成与扩增的pcr引物、方法及应用

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2002503948A (ja) 1995-11-21 2002-02-05 イェール ユニバーシティ 単分子セグメントの増幅および検出
JP2006516410A (ja) 2003-02-03 2006-07-06 ジーイー・ヘルスケア・バイオサイエンス・コーポレイション 発現プロファイリングのためのｃＤＮＡ増幅
US20090099041A1 (en) 2006-02-07 2009-04-16 President And Fellows Of Harvard College Methods for making nucleotide probes for sequencing and synthesis
JP2011505161A (ja) 2007-12-04 2011-02-24 ピエール、ファーブル、メディカマン 抗体ライブラリーを作製およびスクリーニングするための新規な方法
JP2013143966A (ja) 2011-09-08 2013-07-25 Institute Of Physical & Chemical Research プライマーセット及びそれを用いた標的核酸配列の増幅方法並びに変異核酸の検出方法
WO2015089333A1 (en) 2013-12-11 2015-06-18 Accuragen, Inc. Compositions and methods for detecting rare sequence variants

Family Cites Families (150)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US443087A (en) * 1890-12-23 Riding-saddle
US5322770A (en) 1989-12-22 1994-06-21 Hoffman-Laroche Inc. Reverse transcription with thermostable DNA polymerases - high temperature reverse transcription
US5310652A (en) 1986-08-22 1994-05-10 Hoffman-La Roche Inc. Reverse transcription with thermostable DNA polymerase-high temperature reverse transcription
US5130238A (en) 1988-06-24 1992-07-14 Cangene Corporation Enhanced nucleic acid amplification process
US5234809A (en) 1989-03-23 1993-08-10 Akzo N.V. Process for isolating nucleic acid
US6677312B1 (en) 1989-03-29 2004-01-13 The Johns Hopkins University Methods for restoring wild-type p53 gene function
US5527676A (en) 1989-03-29 1996-06-18 The Johns Hopkins University Detection of loss of the wild-type P53 gene and kits therefor
US5362623A (en) 1991-06-14 1994-11-08 The John Hopkins University Sequence specific DNA binding by p53
EP0390323B2 (en) 1989-03-29 2012-08-08 Johns Hopkins University Detection of loss of the wild-type p53 gene
US6800617B1 (en) 1989-03-29 2004-10-05 The Johns Hopkins University Methods for restoring wild-type p53 gene function
WO1991001384A1 (en) 1989-07-11 1991-02-07 Gen-Probe, Incorporated Nucleic acid sequence amplification methods utilizing a transcription complex
CA2020958C (en) 1989-07-11 2005-01-11 Daniel L. Kacian Nucleic acid sequence amplification methods
US5494810A (en) 1990-05-03 1996-02-27 Cornell Research Foundation, Inc. Thermostable ligase-mediated DNA amplifications system for the detection of genetic disease
US5527670A (en) 1990-09-12 1996-06-18 Scientific Generics Limited Electrochemical denaturation of double-stranded nucleic acid
US5352775A (en) 1991-01-16 1994-10-04 The Johns Hopkins Univ. APC gene and nucleic acid probes derived therefrom
AU1366992A (en) 1991-01-16 1992-08-27 Cancer Institute, The Inherited and somatic mutations of apc gene in colorectal cancer of humans
US5455166A (en) 1991-01-31 1995-10-03 Becton, Dickinson And Company Strand displacement amplification
US5330892A (en) 1991-03-13 1994-07-19 The Johns Hopkins University MCC gene (mutated in colorectal cancer) used for diagnosis of cancer in humans
US5270184A (en) 1991-11-19 1993-12-14 Becton, Dickinson And Company Nucleic acid target generation
US5492808A (en) 1993-05-05 1996-02-20 The Johns Hopkins University Means for detecting familial colon cancer (FCC)
US7326778B1 (en) 1993-05-05 2008-02-05 The John Hopkins University Mutator gene and hereditary non-polyposis colorectal cancer
US5591826A (en) 1993-05-05 1997-01-07 The Johns Hopkins University Human MSH2 protein
AU690124B2 (en) 1993-06-09 1998-04-23 Gamera Bioscience Corporation Magnetic cycle reaction
WO1995013375A1 (en) 1993-11-10 1995-05-18 The Johns Hopkins University Tumor suppressor waf1
JP4312827B2 (ja) 1993-12-02 2009-08-12 ザ・ジョーンズ・ホプキンス・ユニバーシティ ヒトのミューテーター遺伝子ｈＭＳＨ２及び遺伝性非ポリープ性大腸ガン
CA2182206A1 (en) 1994-01-27 1995-08-03 William A. Haseltine Human dna mismatch repair proteins
US6482606B1 (en) 1994-01-27 2002-11-19 Human Genome Sciences, Inc. Human DNA mismatch repair polynucleotides
US6380369B1 (en) 1994-01-27 2002-04-30 Human Genome Sciences, Inc. Human DNA mismatch repair proteins
US6033850A (en) 1994-03-15 2000-03-07 Affymetrix, Inc. Electrochemical denaturation of double-stranded nucleic acid
US5648211A (en) 1994-04-18 1997-07-15 Becton, Dickinson And Company Strand displacement amplification using thermophilic enzymes
US6593086B2 (en) * 1996-05-20 2003-07-15 Mount Sinai School Of Medicine Of New York University Nucleic acid amplification methods
US5942391A (en) * 1994-06-22 1999-08-24 Mount Sinai School Of Medicine Nucleic acid amplification method: ramification-extension amplification method (RAM)
US5705628A (en) 1994-09-20 1998-01-06 Whitehead Institute For Biomedical Research DNA purification and isolation using magnetic particles
US5648245A (en) * 1995-05-09 1997-07-15 Carnegie Institution Of Washington Method for constructing an oligonucleotide concatamer library by rolling circle replication
US5705365A (en) 1995-06-07 1998-01-06 Gen-Probe Incorporated Kits for determining pre-amplification levels of a nucleic acid target sequence from post-amplification levels of product
US5710029A (en) 1995-06-07 1998-01-20 Gen-Probe Incorporated Methods for determining pre-amplification levels of a nucleic acid target sequence from post-amplification levels of product
US5854033A (en) 1995-11-21 1998-12-29 Yale University Rolling circle replication reporter systems
US8048629B2 (en) 1996-03-15 2011-11-01 The Penn State Research Foundation Detection of extracellular tumor-associated nucleic acid in blood plasma or serum
AU2324997A (en) 1996-03-15 1997-10-01 Penn State Research Foundation, The Detection of extracellular tumor-associated nucleic acid in blood plasma or ser um using nucleic acid amplification assays
US6511805B1 (en) 1996-03-15 2003-01-28 The Penn State Research Foundation Methods for detecting papillomavirus DNA in blood plasma and serum
DE69739909D1 (de) 1996-03-26 2010-07-29 Michael S Kopreski Methoden aus plasma oder serum extrahierte extrazelluraere rna zur diagnoseüberwachung oder evaluation von krebs verwenden
US5939291A (en) 1996-06-14 1999-08-17 Sarnoff Corporation Microfluidic method for nucleic acid amplification
GB9620209D0 (en) 1996-09-27 1996-11-13 Cemu Bioteknik Ab Method of sequencing DNA
JP2002503954A (ja) 1997-04-01 2002-02-05 グラクソ、グループ、リミテッド 核酸増幅法
GB9706654D0 (en) 1997-04-02 1997-05-21 Scient Generics Ltd Disassociation of interacting molecules
WO1998045474A1 (en) 1997-04-04 1998-10-15 Innogenetics N.V. Isothermal polymerase chain reaction by cycling the concentration of divalent metal ions
WO1999053102A1 (en) * 1998-04-16 1999-10-21 Packard Bioscience Company Analysis of polynucleotide sequence
EP1020534B2 (en) 1998-11-09 2011-01-05 Eiken Kagaku Kabushiki Kaisha Process for synthesizing nucleic acid
GB9903906D0 (en) 1999-02-19 1999-04-14 Microbiological Res Authority Method and apparatus for nucleic acid strand separation
DE60033647T2 (de) 1999-03-17 2007-11-15 The Board Of Trustees Of The Leland Stanford Junior University, Stanford Detektion von fehlbasenpaarungen
CA2364572A1 (en) * 1999-04-12 2000-10-19 Genentech, Inc. Pcr-based cloning method
US20060275782A1 (en) 1999-04-20 2006-12-07 Illumina, Inc. Detection of nucleic acid reactions on bead arrays
US7056661B2 (en) 1999-05-19 2006-06-06 Cornell Research Foundation, Inc. Method for sequencing nucleic acid molecules
AU7364700A (en) 1999-09-08 2001-04-10 University Of Victoria Innovation And Development Corporation Use of psychrotrophic bacterium in biotechnology applications
DK1218542T3 (da) 1999-09-13 2004-08-02 Nugen Technologies Inc Fremgangsmåder og sammensætninger til lineær isotermisk amplifikation af polynukleotidsekvenser
AU7537200A (en) 1999-09-29 2001-04-30 Solexa Ltd. Polynucleotide sequencing
AU783841B2 (en) 1999-11-26 2005-12-15 454 Life Sciences Corporation Nucleic acid probe arrays
US6653079B2 (en) 2000-03-13 2003-11-25 Freshgene, Inc. Methods for detection of differences in nucleic acids
US6917726B2 (en) 2001-09-27 2005-07-12 Cornell Research Foundation, Inc. Zero-mode clad waveguides for performing spectroscopy with confined effective observation volumes
US6626906B1 (en) 2000-10-23 2003-09-30 Sdgi Holdings, Inc. Multi-planar adjustable connector
US7001724B1 (en) 2000-11-28 2006-02-21 Applera Corporation Compositions, methods, and kits for isolating nucleic acids using surfactants and proteases
CA2439477C (en) * 2001-02-27 2014-04-15 Virco Bvba Circular probe amplification (cpa) using energy-transfer primers
MXPA02012739A (es) 2001-03-09 2004-04-20 Nugen Technologies Inc Metodos y composiciones para amplificacion de secuencias de arn.
US7122715B2 (en) * 2001-09-20 2006-10-17 Monsanto Technology Llc In vitro method to create circular molecules for use in transformation
US6977148B2 (en) 2001-10-15 2005-12-20 Qiagen Gmbh Multiple displacement amplification
US20110151438A9 (en) 2001-11-19 2011-06-23 Affymetrix, Inc. Methods of Analysis of Methylation
US7553619B2 (en) * 2002-02-08 2009-06-30 Qiagen Gmbh Detection method using dissociated rolling circle amplification
US6815167B2 (en) * 2002-04-25 2004-11-09 Geneohm Sciences Amplification of DNA to produce single-stranded product of defined sequence and length
CA2498764C (en) 2002-09-20 2015-11-10 New England Biolabs, Inc. Helicase dependent amplification of nucleic acids
US20080021205A1 (en) * 2003-12-11 2008-01-24 Helen Blau Methods and Compositions for Use in Preparing Hairpin Rnas
EP1701785A1 (en) * 2004-01-07 2006-09-20 Solexa Ltd. Modified molecular arrays
AU2005216549A1 (en) 2004-02-27 2005-09-09 President And Fellows Of Harvard College Polony fluorescent in situ sequencing beads
WO2005095654A1 (en) * 2004-03-25 2005-10-13 Biohelix Corporation Helicase-dependent amplification of circular nucleic acids
US8039210B2 (en) 2004-05-14 2011-10-18 The Johns Hopkins University Protein tyrosine phosphatase mutations in cancers
US7170050B2 (en) 2004-09-17 2007-01-30 Pacific Biosciences Of California, Inc. Apparatus and methods for optical analysis of molecules
EP2272984B1 (en) * 2005-02-01 2012-06-20 John Wayne Cancer Institute Use of ID4 for diagnosis and treatment of cancer
WO2006087574A2 (en) 2005-02-19 2006-08-24 Geneform Technologies Limited Isothermal nucleic acid amplification
EP1712639B1 (en) 2005-04-06 2008-08-27 Maurice Stroun Method for the diagnosis of cancer by detecting circulating DNA and RNA
US9139849B2 (en) * 2005-04-08 2015-09-22 The United States of America as Represented by the Government of the Department of Health and Human Services Rapid generation of long synthetic centromeric tandem repeats for mammalian artificial chromosome formation
US20060228721A1 (en) 2005-04-12 2006-10-12 Leamon John H Methods for determining sequence variants using ultra-deep sequencing
EP3257949A1 (en) 2005-06-15 2017-12-20 Complete Genomics Inc. Nucleic acid analysis by random mixtures of non-overlapping fragments
WO2007024653A2 (en) 2005-08-19 2007-03-01 Bioventures, Inc. Method and substances for isolating micro rnas
WO2007064597A2 (en) 2005-11-28 2007-06-07 Pacific Biosciences Of California, Inc. Uniform surfaces for hybrid material substrates and methods for making and using same
US20100291548A1 (en) 2006-03-12 2010-11-18 Applera Corporation Methods of Detecting Target Nucleic Acids
CA2651815A1 (en) 2006-05-10 2007-11-22 Dxterity Diagnostics Detection of nucleic acid targets using chemically reactive oligonucleotide probes
US8768629B2 (en) 2009-02-11 2014-07-01 Caris Mpi, Inc. Molecular profiling of tumors
EP3617321B1 (en) 2006-05-31 2024-10-23 Sequenom, Inc. Kit for the extraction and amplification of nucleic acid from a sample
US7910354B2 (en) 2006-10-27 2011-03-22 Complete Genomics, Inc. Efficient arrays of amplified polynucleotides
AU2007203390B2 (en) 2007-04-26 2012-10-04 Yew Jin Fong Improved lace fastener
EP2396430B1 (en) 2009-02-16 2013-05-01 Epicentre Technologies Corporation Template-independent ligation of single-stranded dna
WO2010117817A2 (en) 2009-03-30 2010-10-14 Life Technologies Corporation Methods for generating target specific probes for solution based capture
CN102625850B (zh) 2009-04-03 2014-11-26 蒂莫西·Z·刘 多重核酸检测方法和系统
EP2419537B1 (en) * 2009-04-13 2014-01-29 Somagenics, Inc. Methods and compositions for detection of small rnas
GB0910302D0 (en) * 2009-06-15 2009-07-29 Lumora Ltd Nucleic acid amplification
US20110237444A1 (en) 2009-11-20 2011-09-29 Life Technologies Corporation Methods of mapping genomic methylation patterns
US8835358B2 (en) 2009-12-15 2014-09-16 Cellular Research, Inc. Digital counting of individual molecules by stochastic attachment of diverse labels
US9315857B2 (en) * 2009-12-15 2016-04-19 Cellular Research, Inc. Digital counting of individual molecules by stochastic attachment of diverse label-tags
US8889350B2 (en) 2010-03-26 2014-11-18 Swift Biosciences, Inc. Methods and compositions for isolating polynucleotides
EP2585593B1 (en) * 2010-06-24 2017-03-01 Population Genetics Technologies Ltd. Methods for polynucleotide library production, immortalization and region of interest extraction
EP2591433A4 (en) 2010-07-06 2017-05-17 Life Technologies Corporation Systems and methods to detect copy number variation
CN102971434B (zh) 2010-08-11 2014-04-09 中国科学院心理研究所 一种甲基化dna的高通量测序方法及其应用
EP2426217A1 (en) 2010-09-03 2012-03-07 Centre National de la Recherche Scientifique (CNRS) Analytical methods for cell free nucleic acids and applications
CN101985654A (zh) 2010-10-21 2011-03-16 徐州医学院 检测多样本多位点snp的检测方法
ES2986436T3 (es) 2011-04-15 2024-11-11 Univ Johns Hopkins Sistema de secuenciación segura
WO2014014498A1 (en) 2012-07-20 2014-01-23 Verinata Health, Inc. Detecting and classifying copy number variation in a fetal genome
WO2013003585A2 (en) 2011-06-29 2013-01-03 The Johns Hopkins University Enrichment of nucleic acids by complimentary capture
CN103717752B (zh) * 2011-08-05 2015-04-15 株式会社东芝 核酸分析法
WO2013056178A2 (en) 2011-10-14 2013-04-18 Foundation Medicine, Inc. Novel estrogen receptor mutations and uses thereof
WO2013059740A1 (en) 2011-10-21 2013-04-25 Foundation Medicine, Inc. Novel alk and ntrk1 fusion molecules and uses thereof
WO2013074632A1 (en) 2011-11-14 2013-05-23 Rose Floyd D Mismatch nucleotide purification and identification
US20130217023A1 (en) 2012-02-22 2013-08-22 454 Life Sciences Corporation System And Method For Generation And Use Of Compact Clonally Amplified Products
PT2828218T (pt) 2012-03-20 2020-11-11 Univ Washington Through Its Center For Commercialization Métodos para baixar a taxa de erro da sequenciação paralela massiva de adn utilizando sequenciação duplex de consensus
US8209130B1 (en) 2012-04-04 2012-06-26 Good Start Genetics, Inc. Sequence assembly
AU2013267609C1 (en) 2012-05-31 2019-01-03 Board Of Regents, The University Of Texas System Method for accurate sequencing of DNA
US10538807B2 (en) 2012-06-01 2020-01-21 Fred Hutchinson Cancer Research Center Compositions and methods for detecting rare nucleic acid molecule mutations
US20150126376A1 (en) 2012-06-14 2015-05-07 Fred Hutchinson Cancer Research Center Compositions and methods for sensitive mutation detection in nucleic acid molecules
CN103998621A (zh) 2012-07-11 2014-08-20 北京贝瑞和康生物技术有限公司 片断dna检测方法、片断dna检测试剂盒及其应用
CA2876505A1 (en) 2012-07-17 2014-01-23 Counsyl, Inc. System and methods for detecting genetic variation
US9092401B2 (en) 2012-10-31 2015-07-28 Counsyl, Inc. System and methods for detecting genetic variation
US20160040229A1 (en) 2013-08-16 2016-02-11 Guardant Health, Inc. Systems and methods to detect rare mutations and copy number variation
US20140066317A1 (en) 2012-09-04 2014-03-06 Guardant Health, Inc. Systems and methods to detect rare mutations and copy number variation
EP2914753A4 (en) 2012-10-31 2016-06-08 Becton Dickinson Co SELECTIVE AMPLIFICATION AND REAL-TIME PCR DETECTION OF RARE MUTATIONS
CA3150658A1 (en) 2013-01-18 2014-07-24 Foundation Medicine, Inc. Methods of treating cholangiocarcinoma
WO2014151117A1 (en) 2013-03-15 2014-09-25 The Board Of Trustees Of The Leland Stanford Junior University Identification and use of circulating nucleic acid tumor markers
US10081825B2 (en) * 2013-03-15 2018-09-25 Aegea Biotechnologies, Inc. Methods for amplification of nucleic acids utilizing a circularized template prepared from a target nucleic acid
US9290800B2 (en) * 2013-03-15 2016-03-22 Pacific Biosciences Of California, Inc. Targeted rolling circle amplification
WO2014145078A1 (en) 2013-03-15 2014-09-18 Verinata Health, Inc. Generating cell-free dna libraries directly from blood
US20150033372A1 (en) * 2013-05-01 2015-01-29 Kymab Limited Human VpreB & Chimaeric Surrogate Light Chains in Transgenic Non-Human Vertebrates
US9217167B2 (en) 2013-07-26 2015-12-22 General Electric Company Ligase-assisted nucleic acid circularization and amplification
US9644232B2 (en) 2013-07-26 2017-05-09 General Electric Company Method and device for collection and amplification of circulating nucleic acids
US9499870B2 (en) 2013-09-27 2016-11-22 Natera, Inc. Cell free DNA diagnostic testing standards
JP6602294B2 (ja) * 2013-10-17 2019-11-06 タカラ バイオ ユーエスエー， インコーポレイテッド アダプタを核酸に追加する方法、及びこの方法を実行するための組成物
AU2014346562B2 (en) 2013-11-07 2018-11-29 The Board Of Trustees Of The Leland Stanford Junior University Cell-free nucleic acids for the analysis of the human microbiome and components thereof
GB201321123D0 (en) 2013-11-29 2014-01-15 Linea Ab Q Amplification of circular molecules
US11286519B2 (en) 2013-12-11 2022-03-29 Accuragen Holdings Limited Methods and compositions for enrichment of amplification products
US11859246B2 (en) 2013-12-11 2024-01-02 Accuragen Holdings Limited Methods and compositions for enrichment of amplification products
WO2017062863A1 (en) 2015-10-09 2017-04-13 Accuragen Holdings Limited Methods and compositions for enrichment of amplification products
ES2660989T3 (es) 2013-12-28 2018-03-27 Guardant Health, Inc. Métodos y sistemas para detectar variantes genéticas
CN104745679B (zh) 2013-12-31 2018-06-15 杭州贝瑞和康基因诊断技术有限公司 一种无创检测egfr基因突变的方法及试剂盒
EP3363904B1 (en) * 2014-01-31 2019-10-23 Swift Biosciences, Inc. Improved methods for processing dna substrates
CN106715693A (zh) * 2014-06-13 2017-05-24 伊卢米纳剑桥有限公司 用于制备序列文库的方法和组合物
US10364458B2 (en) * 2014-07-16 2019-07-30 Tangen Biosciences, Inc. Isothermal methods for amplifying nucleic acid samples
US11987836B2 (en) 2014-09-30 2024-05-21 Global Life Sciences Solutions Usa Llc Method for nucleic acid analysis directly from an unpurified biological sample
US10844428B2 (en) 2015-04-28 2020-11-24 Illumina, Inc. Error suppression in sequenced DNA fragments using redundant reads with unique molecular indices (UMIS)
WO2017096322A1 (en) 2015-12-03 2017-06-08 Accuragen Holdings Limited Methods and compositions for forming ligation products
CN109511265B (zh) 2016-05-16 2023-07-14 安可济控股有限公司 通过链鉴定改进测序的方法
US10370701B2 (en) * 2016-06-17 2019-08-06 Pacific Biosciences Of California, Inc. Methods and compositions for generating asymmetrically-tagged nucleic acid fragments
EP3475449B1 (en) 2016-06-23 2022-08-17 Accuragen Holdings Limited Uses of a cell-free nucleic acid standards
CN109844133A (zh) 2016-08-15 2019-06-04 安可济控股有限公司 检测罕见序列变体的组合物和方法
US11203782B2 (en) 2018-03-29 2021-12-21 Accuragen Holdings Limited Compositions and methods comprising asymmetric barcoding

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2002503948A (ja) 1995-11-21 2002-02-05 イェール ユニバーシティ 単分子セグメントの増幅および検出
JP2006516410A (ja) 2003-02-03 2006-07-06 ジーイー・ヘルスケア・バイオサイエンス・コーポレイション 発現プロファイリングのためのｃＤＮＡ増幅
US20090099041A1 (en) 2006-02-07 2009-04-16 President And Fellows Of Harvard College Methods for making nucleotide probes for sequencing and synthesis
JP2011505161A (ja) 2007-12-04 2011-02-24 ピエール、ファーブル、メディカマン 抗体ライブラリーを作製およびスクリーニングするための新規な方法
JP2013143966A (ja) 2011-09-08 2013-07-25 Institute Of Physical & Chemical Research プライマーセット及びそれを用いた標的核酸配列の増幅方法並びに変異核酸の検出方法
WO2015089333A1 (en) 2013-12-11 2015-06-18 Accuragen, Inc. Compositions and methods for detecting rare sequence variants

Also Published As

Publication number Publication date
IL287586A (en) 2021-12-01
WO2017062863A1 (en) 2017-04-13
US11578359B2 (en) 2023-02-14
AU2023201912A1 (en) 2023-05-04
US10752942B2 (en) 2020-08-25
EP3359693B1 (en) 2025-03-19
CN114807323A (zh) 2022-07-29
MX2024002873A (es) 2024-03-22
MX2018004284A (es) 2018-05-16
US20230287485A1 (en) 2023-09-14
US20210002716A1 (en) 2021-01-07
EP3359693A4 (en) 2019-03-06
SG11201802864RA (en) 2018-05-30
CN108368545B (zh) 2022-05-17
CA2999902A1 (en) 2017-04-13
CN108368545A (zh) 2018-08-03
JP2022050563A (ja) 2022-03-30
AU2016334233B2 (en) 2023-01-05
HK1259101A1 (zh) 2019-11-22
IL258236A (en) 2018-05-31
JP2018537955A (ja) 2018-12-27
IL258236B (en) 2021-12-01
EP3359693A1 (en) 2018-08-15
US20180298434A1 (en) 2018-10-18
KR20180055905A (ko) 2018-05-25
AU2016334233A1 (en) 2018-04-12
JP2024099542A (ja) 2024-07-25

Similar Documents

Publication Publication Date Title
JP7008016B2 (ja) 増幅産生物の富化のための方法および組成物
JP7365382B2 (ja) 稀な配列変異体を検出するための組成物および方法
US12152271B2 (en) Methods and compositions for preparing polynucleotides
KR102379877B1 (ko) 희귀 서열 변이를 검출하기 위한 조성물 및 방법
JP2024060054A (ja) ヌクレアーゼ、リガーゼ、ポリメラーゼ、及び配列決定反応の組み合わせを用いた、核酸配列、発現、コピー、またはｄｎａのメチル化変化の識別及び計数方法
JP6410726B2 (ja) 標的化ゲノム解析のための方法
US20180363039A1 (en) Methods and compositions for forming ligation products
JP2024096852A (ja) 核酸ライブラリー作製のための組成物及び方法
US12043867B2 (en) Compositions and methods comprising asymmetric barcoding
US11634750B2 (en) Methods and compositions for preparing polynucleotides
HK40016380A (en) Compositions and methods for preparing nucleic acid libraries

Legal Events

Date Code Title Description
A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20180606

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20191003

A621 Written request for application examination

Free format text: JAPANESE INTERMEDIATE CODE: A621

Effective date: 20191003

A977 Report on retrieval

Free format text: JAPANESE INTERMEDIATE CODE: A971007

Effective date: 20200812

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20200821

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20201120

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20210416

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20210716

TRDD Decision of grant or rejection written
A01 Written decision to grant a patent or to grant a registration (utility model)

Free format text: JAPANESE INTERMEDIATE CODE: A01

Effective date: 20211208

A61 First payment of annual fees (during grant procedure)

Free format text: JAPANESE INTERMEDIATE CODE: A61

Effective date: 20220107

R150 Certificate of patent or registration of utility model

Ref document number: 7008016

Country of ref document: JP

Free format text: JAPANESE INTERMEDIATE CODE: R150

RD02 Notification of acceptance of power of attorney

Free format text: JAPANESE INTERMEDIATE CODE: R3D02

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250