JP7002557B2 - エクオール誘導体を生産する組換え大腸菌およびこれを利用したエクオール誘導体の合成方法 - Google Patents

エクオール誘導体を生産する組換え大腸菌およびこれを利用したエクオール誘導体の合成方法 Download PDF

Info

Publication number
JP7002557B2
JP7002557B2 JP2019546813A JP2019546813A JP7002557B2 JP 7002557 B2 JP7002557 B2 JP 7002557B2 JP 2019546813 A JP2019546813 A JP 2019546813A JP 2019546813 A JP2019546813 A JP 2019546813A JP 7002557 B2 JP7002557 B2 JP 7002557B2
Authority
JP
Japan
Prior art keywords
equol
reductase
hydroxy
dihydrodaidzein
escherichia coli
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
JP2019546813A
Other languages
English (en)
Japanese (ja)
Other versions
JP2020508671A (ja
JP2020508671A5 (enExample
Inventor
ジー キム，ビョン
イ，ピョン－ガン
ヒョク イ，サン
ウォン キム，ジュン
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
SNU R&DB Foundation
Original Assignee
Seoul National University R&DB Foundation
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Seoul National University R&DB Foundation filed Critical Seoul National University R&DB Foundation
Publication of JP2020508671A publication Critical patent/JP2020508671A/ja
Publication of JP2020508671A5 publication Critical patent/JP2020508671A5/ja
Application granted granted Critical
Publication of JP7002557B2 publication Critical patent/JP7002557B2/ja
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Images

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/001—Oxidoreductases (1.) acting on the CH-CH group of donors (1.3)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/90—Isomerases (5.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P17/00—Preparation of heterocyclic carbon compounds with only O, N, S, Se or Te as ring hetero atoms
    • C12P17/02—Oxygen as only ring hetero atoms
    • C12P17/06—Oxygen as only ring hetero atoms containing a six-membered hetero ring, e.g. fluorescein
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y103/00—Oxidoreductases acting on the CH-CH group of donors (1.3)
    • C12Y103/01—Oxidoreductases acting on the CH-CH group of donors (1.3) with NAD+ or NADP+ as acceptor (1.3.1)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
JP2019546813A 2017-02-28 2018-02-22 エクオール誘導体を生産する組換え大腸菌およびこれを利用したエクオール誘導体の合成方法 Active JP7002557B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
KR1020170026774A KR102005237B1 (ko) 2017-02-28 2017-02-28 에쿠올 유도체를 생산하는 재조합 대장균 및 이를 이용한 에쿠올 유도체 합성 방법
KR10-2017-0026774 2017-02-28
PCT/KR2018/002201 WO2018159957A1 (ko) 2017-02-28 2018-02-22 에쿠올 유도체를 생산하는 재조합 대장균 및 이를 이용한 에쿠올 유도체 합성 방법

Publications (3)

Publication Number Publication Date
JP2020508671A JP2020508671A (ja) 2020-03-26
JP2020508671A5 JP2020508671A5 (enExample) 2020-05-07
JP7002557B2 true JP7002557B2 (ja) 2022-02-10

Family

ID=63370438

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2019546813A Active JP7002557B2 (ja) 2017-02-28 2018-02-22 エクオール誘導体を生産する組換え大腸菌およびこれを利用したエクオール誘導体の合成方法

Country Status (4)

Country Link
JP (1) JP7002557B2 (enExample)
KR (1) KR102005237B1 (enExample)
CN (1) CN110382701A (enExample)
WO (1) WO2018159957A1 (enExample)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR102079003B1 (ko) * 2018-08-10 2020-02-19 서울대학교산학협력단 활성이 증대된 테트라하이드로다이드제인 환원효소 및 에쿠올 유도체 생산에의 응용
JP7449526B2 (ja) * 2018-12-07 2024-03-14 学校法人東京理科大学 エクオール誘導体の産生のための組成物
KR102222517B1 (ko) 2019-03-26 2021-03-03 서울대학교산학협력단 모노옥시게나아제를 포함하는 생촉매 조성물 및 이를 이용한 에쿠올 수산화 유도체의 제조 방법
CN114806999B (zh) * 2022-06-30 2022-09-27 华熙生物科技股份有限公司 一种基因工程菌及其在制备二氢大豆苷元中的应用
CN115725614B (zh) * 2022-08-05 2025-06-06 华熙生物科技股份有限公司 一种生产雌马酚的菌株及其应用
WO2025181921A1 (ja) * 2024-02-27 2025-09-04 株式会社ダイセル エクオールおよび５－ヒドロキシエクオールの製造方法

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2009084603A1 (ja) 2007-12-27 2009-07-09 Otsuka Pharmaceutical Co., Ltd. エクオール合成に関与する酵素
CN104031875A (zh) 2014-05-30 2014-09-10 浙江省农业科学院 一种s-雌马酚产生工程菌及应用

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP4256679B2 (ja) * 2001-03-16 2009-04-22 ノボゲン リサーチ ピーティーワイ リミテッド 再狭窄の治療方法
CN101123958A (zh) * 2003-11-19 2008-02-13 诺沃根研究股份有限公司 联合放射疗法与化学疗法的组合物和方法
CN103275884B (zh) 2013-02-04 2014-08-20 河北农业大学 史雷克氏菌auh-jlc159及其转化制备(-)-5-oh-雌马酚的方法

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2009084603A1 (ja) 2007-12-27 2009-07-09 Otsuka Pharmaceutical Co., Ltd. エクオール合成に関与する酵素
CN104031875A (zh) 2014-05-30 2014-09-10 浙江省农业科学院 一种s-雌马酚产生工程菌及应用

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
Applied and Environmental Microbiology，2012年，Vol.78, No.14，pp.4902-4907
Applied and Environmental Microbiology，2013年，Vol.79, No.11，pp.3494-3502
Applied and Environmental Microbiology，2016年，Vol.82, No.7，pp.1992-2002
The Journal of Nutrition，2011年，Vol.142，pp.40-46

Also Published As

Publication number Publication date
KR20180099380A (ko) 2018-09-05
JP2020508671A (ja) 2020-03-26
WO2018159957A1 (ko) 2018-09-07
KR102005237B1 (ko) 2019-07-30
CN110382701A (zh) 2019-10-25

Similar Documents

Publication Publication Date Title
JP7002557B2 (ja) エクオール誘導体を生産する組換え大腸菌およびこれを利用したエクオール誘導体の合成方法
JP7156594B2 (ja) １ステップ発酵による（ｒ）－３－ヒドロキシ酪酸またはその塩の調製
Murata et al. Oxidative rearrangement of (+)-sesamin by CYP92B14 co-generates twin dietary lignans in sesame
Blasco et al. CYP175A1 from Thermus thermophilus HB27, the first β-carotene hydroxylase of the P450 superfamily
Eustáquio et al. Biosynthetic engineering and fermentation media development leads to gram-scale production of spliceostatin natural products in Burkholderia sp.
CN105555776B (zh) 利用酪氨酸酶制备的儿茶酚型结构物质、其制备方法及其应用
Černuchová et al. Microbial Baeyer–Villiger oxidation of terpenones by recombinant whole-cell biocatalysts—formation of enantiocomplementary regioisomeric lactones
Frensch et al. Enzymatic spiroketal formation via oxidative rearrangement of pentangular polyketides
CN105441371B (zh) 一种基因工程菌及其在生产辅酶q10中的应用
Hartz et al. Characterization and engineering of a carotenoid biosynthesis operon from Bacillus megaterium
Liu et al. Efficient microbial synthesis of key steroidal intermediates from bio-renewable phytosterols by genetically modified Mycobacterium fortuitum strains
CA2546614A1 (en) Dna participating in hydroxylation of macrolide compound
JP5474360B2 (ja) 新規大環状ラクトン
TW200846473A (en) Method for preparing (4S)-3,4-dihydroxy-2,6,6-trimethylcyclohex-2-enone and derivatives thereof
EP3034610A1 (en) Novel terpenoid compound and method for producing same
JPH10130269A (ja) カルボリン誘導体
KR102079003B1 (ko) 활성이 증대된 테트라하이드로다이드제인 환원효소 및 에쿠올 유도체 생산에의 응용
CN110885281B (zh) 一类四环二萜类化合物及其制备方法与应用
KR102326617B1 (ko) 인돌렌인온의 제조 방법 및 인돌렌인온 합성을 통한 인디루빈 유도체의 제조 방법
JP2023539983A (ja) 改変されたテルペンシンターゼならびにプソイドプテロシン中間体および／またはプソイドプテロシンの産生のためのそれらの使用
KR20200113575A (ko) 모노옥시게나아제를 포함하는 생촉매 조성물 및 이를 이용한 에쿠올 수산화 유도체의 제조 방법
EP2054510A2 (de) Verfahren zur herstellung von optisch aktiven hydroxyalkylacetaten durch racematspaltung von 4-hydroxy-2-ketonen unter verwendung von baeyer-villiger monooxygenasen
CN101006184B (zh) 甾醇的5-烯-3-酮衍生物或3,6-二酮衍生物的制造方法，脂质代谢改善剂、饮食品以及动物饲料的制造方法，和分析方法
JP7545614B1 (ja) エクオールおよび５－ヒドロキシエクオールの製造方法
JP2001031564A (ja) テロメレース阻害剤

Legal Events

Date Code Title Description
A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20190828

A621 Written request for application examination

Free format text: JAPANESE INTERMEDIATE CODE: A621

Effective date: 20190828

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20200908

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20201120

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20210413

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20210705

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20211005

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20211122

TRDD Decision of grant or rejection written
A01 Written decision to grant a patent or to grant a registration (utility model)

Free format text: JAPANESE INTERMEDIATE CODE: A01

Effective date: 20211207

A61 First payment of annual fees (during grant procedure)

Free format text: JAPANESE INTERMEDIATE CODE: A61

Effective date: 20211227

R150 Certificate of patent or registration of utility model

Ref document number: 7002557

Country of ref document: JP

Free format text: JAPANESE INTERMEDIATE CODE: R150

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250