CN110382701A - 生产雌马酚衍生物的重组大肠杆菌及利用其的雌马酚衍生物合成方法 - Google Patents

生产雌马酚衍生物的重组大肠杆菌及利用其的雌马酚衍生物合成方法 Download PDF

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Publication number
CN110382701A
CN110382701A CN201880014592.9A CN201880014592A CN110382701A CN 110382701 A CN110382701 A CN 110382701A CN 201880014592 A CN201880014592 A CN 201880014592A CN 110382701 A CN110382701 A CN 110382701A
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hydroxy
equol
reductase
dehydroequol
dihydrodaidzein
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CN201880014592.9A
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English (en)
Chinese (zh)
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金秉祺
李平康
李相赫
金濬园
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Seoul University School-Industry Cooperation Group
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Seoul University School-Industry Cooperation Group
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/001—Oxidoreductases (1.) acting on the CH-CH group of donors (1.3)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/90—Isomerases (5.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P17/00—Preparation of heterocyclic carbon compounds with only O, N, S, Se or Te as ring hetero atoms
    • C12P17/02—Oxygen as only ring hetero atoms
    • C12P17/06—Oxygen as only ring hetero atoms containing a six-membered hetero ring, e.g. fluorescein
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y103/00—Oxidoreductases acting on the CH-CH group of donors (1.3)
    • C12Y103/01—Oxidoreductases acting on the CH-CH group of donors (1.3) with NAD+ or NADP+ as acceptor (1.3.1)

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
CN201880014592.9A 2017-02-28 2018-02-22 生产雌马酚衍生物的重组大肠杆菌及利用其的雌马酚衍生物合成方法 Pending CN110382701A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
KR1020170026774A KR102005237B1 (ko) 2017-02-28 2017-02-28 에쿠올 유도체를 생산하는 재조합 대장균 및 이를 이용한 에쿠올 유도체 합성 방법
KR10-2017-0026774 2017-02-28
PCT/KR2018/002201 WO2018159957A1 (ko) 2017-02-28 2018-02-22 에쿠올 유도체를 생산하는 재조합 대장균 및 이를 이용한 에쿠올 유도체 합성 방법

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CN110382701A true CN110382701A (zh) 2019-10-25

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CN201880014592.9A Pending CN110382701A (zh) 2017-02-28 2018-02-22 生产雌马酚衍生物的重组大肠杆菌及利用其的雌马酚衍生物合成方法

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JP (1) JP7002557B2 (enExample)
KR (1) KR102005237B1 (enExample)
CN (1) CN110382701A (enExample)
WO (1) WO2018159957A1 (enExample)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114806999A (zh) * 2022-06-30 2022-07-29 华熙生物科技股份有限公司 一种基因工程菌及其在制备二氢大豆苷元中的应用
CN115725614A (zh) * 2022-08-05 2023-03-03 华熙生物科技股份有限公司 一种生产雌马酚的菌株及其应用

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR102079003B1 (ko) * 2018-08-10 2020-02-19 서울대학교산학협력단 활성이 증대된 테트라하이드로다이드제인 환원효소 및 에쿠올 유도체 생산에의 응용
JP7449526B2 (ja) * 2018-12-07 2024-03-14 学校法人東京理科大学 エクオール誘導体の産生のための組成物
KR102222517B1 (ko) 2019-03-26 2021-03-03 서울대학교산학협력단 모노옥시게나아제를 포함하는 생촉매 조성물 및 이를 이용한 에쿠올 수산화 유도체의 제조 방법
WO2025181921A1 (ja) * 2024-02-27 2025-09-04 株式会社ダイセル エクオールおよび５－ヒドロキシエクオールの製造方法

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104031875A (zh) * 2014-05-30 2014-09-10 浙江省农业科学院 一种s-雌马酚产生工程菌及应用

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JP4256679B2 (ja) * 2001-03-16 2009-04-22 ノボゲン リサーチ ピーティーワイ リミテッド 再狭窄の治療方法
CN101123958A (zh) * 2003-11-19 2008-02-13 诺沃根研究股份有限公司 联合放射疗法与化学疗法的组合物和方法
DK2404995T3 (da) * 2007-12-27 2013-10-14 Otsuka Pharma Co Ltd Enzym associeret med equolsyntese
CN103275884B (zh) 2013-02-04 2014-08-20 河北农业大学 史雷克氏菌auh-jlc159及其转化制备(-)-5-oh-雌马酚的方法

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104031875A (zh) * 2014-05-30 2014-09-10 浙江省农业科学院 一种s-雌马酚产生工程菌及应用

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
CHRISTINE SCHRÖDER等: "Identification and Expression of Genes Involved in the Conversion of Daidzein and Genistein by the Equol-Forming Bacterium Slackia isoflavoniconvertens.", 《 APPLIED AND ENVIRONMENTAL MICROBIOLOGY》 *
PYUNG-GANG LEE等: "P212A Mutant of Dihydrodaidzein Reductase Enhances (S)-Equol Production and Enantioselectivity in a Recombinant Escherichia coli Whole-Cell Reaction System.", 《APPLIED AND ENVIRONMENTAL MICROBIOLOGY》 *

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114806999A (zh) * 2022-06-30 2022-07-29 华熙生物科技股份有限公司 一种基因工程菌及其在制备二氢大豆苷元中的应用
CN115725614A (zh) * 2022-08-05 2023-03-03 华熙生物科技股份有限公司 一种生产雌马酚的菌株及其应用

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KR20180099380A (ko) 2018-09-05
JP2020508671A (ja) 2020-03-26
JP7002557B2 (ja) 2022-02-10
WO2018159957A1 (ko) 2018-09-07
KR102005237B1 (ko) 2019-07-30

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