KR102005237B1 - 에쿠올 유도체를 생산하는 재조합 대장균 및 이를 이용한 에쿠올 유도체 합성 방법 - Google Patents

에쿠올 유도체를 생산하는 재조합 대장균 및 이를 이용한 에쿠올 유도체 합성 방법 Download PDF

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KR102005237B1
KR102005237B1 KR1020170026774A KR20170026774A KR102005237B1 KR 102005237 B1 KR102005237 B1 KR 102005237B1 KR 1020170026774 A KR1020170026774 A KR 1020170026774A KR 20170026774 A KR20170026774 A KR 20170026774A KR 102005237 B1 KR102005237 B1 KR 102005237B1
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reductase
hydroxy
equol
recombinant
dihydrodaidzein
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KR20180099380A (ko
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김병기
이평강
이상혁
김준원
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서울대학교산학협력단
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Priority to KR1020170026774A priority Critical patent/KR102005237B1/ko
Priority to CN201880014592.9A priority patent/CN110382701A/zh
Priority to PCT/KR2018/002201 priority patent/WO2018159957A1/ko
Priority to JP2019546813A priority patent/JP7002557B2/ja
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/001—Oxidoreductases (1.) acting on the CH-CH group of donors (1.3)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/90—Isomerases (5.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P17/00—Preparation of heterocyclic carbon compounds with only O, N, S, Se or Te as ring hetero atoms
    • C12P17/02—Oxygen as only ring hetero atoms
    • C12P17/06—Oxygen as only ring hetero atoms containing a six-membered hetero ring, e.g. fluorescein
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y103/00—Oxidoreductases acting on the CH-CH group of donors (1.3)
    • C12Y103/01—Oxidoreductases acting on the CH-CH group of donors (1.3) with NAD+ or NADP+ as acceptor (1.3.1)

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
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  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Physics & Mathematics (AREA)
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  • Plant Pathology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
KR1020170026774A 2017-02-28 2017-02-28 에쿠올 유도체를 생산하는 재조합 대장균 및 이를 이용한 에쿠올 유도체 합성 방법 Active KR102005237B1 (ko)

Priority Applications (4)

Application Number Priority Date Filing Date Title
KR1020170026774A KR102005237B1 (ko) 2017-02-28 2017-02-28 에쿠올 유도체를 생산하는 재조합 대장균 및 이를 이용한 에쿠올 유도체 합성 방법
CN201880014592.9A CN110382701A (zh) 2017-02-28 2018-02-22 生产雌马酚衍生物的重组大肠杆菌及利用其的雌马酚衍生物合成方法
PCT/KR2018/002201 WO2018159957A1 (ko) 2017-02-28 2018-02-22 에쿠올 유도체를 생산하는 재조합 대장균 및 이를 이용한 에쿠올 유도체 합성 방법
JP2019546813A JP7002557B2 (ja) 2017-02-28 2018-02-22 エクオール誘導体を生産する組換え大腸菌およびこれを利用したエクオール誘導体の合成方法

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
KR1020170026774A KR102005237B1 (ko) 2017-02-28 2017-02-28 에쿠올 유도체를 생산하는 재조합 대장균 및 이를 이용한 에쿠올 유도체 합성 방법

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KR20180099380A KR20180099380A (ko) 2018-09-05
KR102005237B1 true KR102005237B1 (ko) 2019-07-30

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JP (1) JP7002557B2 (enExample)
KR (1) KR102005237B1 (enExample)
CN (1) CN110382701A (enExample)
WO (1) WO2018159957A1 (enExample)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR102079003B1 (ko) * 2018-08-10 2020-02-19 서울대학교산학협력단 활성이 증대된 테트라하이드로다이드제인 환원효소 및 에쿠올 유도체 생산에의 응용
JP7449526B2 (ja) * 2018-12-07 2024-03-14 学校法人東京理科大学 エクオール誘導体の産生のための組成物
KR102222517B1 (ko) 2019-03-26 2021-03-03 서울대학교산학협력단 모노옥시게나아제를 포함하는 생촉매 조성물 및 이를 이용한 에쿠올 수산화 유도체의 제조 방법
CN114806999B (zh) * 2022-06-30 2022-09-27 华熙生物科技股份有限公司 一种基因工程菌及其在制备二氢大豆苷元中的应用
CN115725614B (zh) * 2022-08-05 2025-06-06 华熙生物科技股份有限公司 一种生产雌马酚的菌株及其应用
WO2025181921A1 (ja) * 2024-02-27 2025-09-04 株式会社ダイセル エクオールおよび５－ヒドロキシエクオールの製造方法

Citations (1)

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US20060167037A1 (en) * 2003-11-19 2006-07-27 Kelly Graham E Combinational radiotherapy and chemotherapy compositions and methods

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EP1368024A4 (en) * 2001-03-16 2009-03-18 Novogen Res Pty Ltd TREATMENT OF RESTENOSIS
ES2424470T3 (es) * 2007-12-27 2013-10-02 Otsuka Pharmaceutical Co., Ltd. Enzima asociado a la síntesis de ecuol
CN103275884B (zh) 2013-02-04 2014-08-20 河北农业大学 史雷克氏菌auh-jlc159及其转化制备(-)-5-oh-雌马酚的方法
CN104031875B (zh) * 2014-05-30 2016-04-20 浙江省农业科学院 一种s-雌马酚产生工程菌及应用

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20060167037A1 (en) * 2003-11-19 2006-07-27 Kelly Graham E Combinational radiotherapy and chemotherapy compositions and methods

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KR20180099380A (ko) 2018-09-05
JP7002557B2 (ja) 2022-02-10
WO2018159957A1 (ko) 2018-09-07
CN110382701A (zh) 2019-10-25
JP2020508671A (ja) 2020-03-26

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