JP6998863B2 - 深共融溶媒におけるソルターゼaを利用したアミド基転移 - Google Patents
深共融溶媒におけるソルターゼaを利用したアミド基転移 Download PDFInfo
- Publication number
- JP6998863B2 JP6998863B2 JP2018515654A JP2018515654A JP6998863B2 JP 6998863 B2 JP6998863 B2 JP 6998863B2 JP 2018515654 A JP2018515654 A JP 2018515654A JP 2018515654 A JP2018515654 A JP 2018515654A JP 6998863 B2 JP6998863 B2 JP 6998863B2
- Authority
- JP
- Japan
- Prior art keywords
- amino acid
- sortase
- polypeptide
- acid sequence
- seq
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
- C12Y304/22—Cysteine endopeptidases (3.4.22)
- C12Y304/2207—Sortase A (3.4.22.70)
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- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Health & Medical Sciences (AREA)
- Zoology (AREA)
- General Chemical & Material Sciences (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Microbiology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- Molecular Biology (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Enzymes And Modification Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2021172120A JP2022025094A (ja) | 2015-09-25 | 2021-10-21 | 深共融溶媒におけるソルターゼaを利用したアミド基転移 |
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP15186955 | 2015-09-25 | ||
| EP15186955.9 | 2015-09-25 | ||
| EP16172623.7 | 2016-06-02 | ||
| EP16172623 | 2016-06-02 | ||
| PCT/EP2016/072510 WO2017050872A1 (en) | 2015-09-25 | 2016-09-22 | Transamidation employing sortase a in deep eutectic solvents |
Related Child Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2021172120A Division JP2022025094A (ja) | 2015-09-25 | 2021-10-21 | 深共融溶媒におけるソルターゼaを利用したアミド基転移 |
Publications (4)
| Publication Number | Publication Date |
|---|---|
| JP2018531009A JP2018531009A (ja) | 2018-10-25 |
| JP2018531009A6 JP2018531009A6 (ja) | 2018-12-13 |
| JP2018531009A5 JP2018531009A5 (enExample) | 2019-10-31 |
| JP6998863B2 true JP6998863B2 (ja) | 2022-02-04 |
Family
ID=57068066
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2018515654A Expired - Fee Related JP6998863B2 (ja) | 2015-09-25 | 2016-09-22 | 深共融溶媒におけるソルターゼaを利用したアミド基転移 |
| JP2021172120A Pending JP2022025094A (ja) | 2015-09-25 | 2021-10-21 | 深共融溶媒におけるソルターゼaを利用したアミド基転移 |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2021172120A Pending JP2022025094A (ja) | 2015-09-25 | 2021-10-21 | 深共融溶媒におけるソルターゼaを利用したアミド基転移 |
Country Status (5)
| Country | Link |
|---|---|
| US (1) | US11174502B2 (enExample) |
| EP (1) | EP3353314A1 (enExample) |
| JP (2) | JP6998863B2 (enExample) |
| CN (1) | CN108026560A (enExample) |
| WO (1) | WO2017050872A1 (enExample) |
Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR102220167B1 (ko) * | 2018-09-04 | 2021-02-25 | 성균관대학교산학협력단 | 단백질 상온 안정화 및 저장용 천연공융용매 |
| CN113069889A (zh) * | 2021-04-22 | 2021-07-06 | 北京工业大学 | 一种利用低共熔溶剂捕集甲苯的方法 |
| CN114113023B (zh) * | 2021-12-16 | 2024-04-26 | 郑州轻工业大学 | 基于单核增生李斯特菌来源的氮掺杂碳点的制备方法和应用 |
| CN114525313B (zh) * | 2022-02-25 | 2024-04-19 | 广州市宝桃食品有限公司 | 一种酶法合成丙二醇脂肪酸单酯的方法 |
| CN115594778B (zh) * | 2022-11-23 | 2023-04-11 | 中国海洋大学 | 利用酸性低共熔溶剂提取甲壳素的方法 |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20090117628A1 (en) | 2007-09-21 | 2009-05-07 | Gorke Johnathan T | Enzymatic processing in deep eutectic solvents |
| WO2013016653A1 (en) | 2011-07-28 | 2013-01-31 | Cell Signaling Technology, Inc. | Multi component detection |
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| US4150149A (en) | 1976-11-29 | 1979-04-17 | Professional Staff Association Of The Los Angeles County Harbor General Hospital | Method and means for the early detection and diagnosis of certain types of cancers |
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| US5342606A (en) | 1984-10-18 | 1994-08-30 | Board Of Regents, The University Of Texas System | Polyazamacrocyclic compounds for complexation of metal ions |
| US5316757A (en) | 1984-10-18 | 1994-05-31 | Board Of Regents, The University Of Texas System | Synthesis of polyazamacrocycles with more than one type of side-chain chelating groups |
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| US5959177A (en) | 1989-10-27 | 1999-09-28 | The Scripps Research Institute | Transgenic plants expressing assembled secretory antibodies |
| JP3951062B2 (ja) | 1991-09-19 | 2007-08-01 | ジェネンテック・インコーポレーテッド | 少なくとも遊離のチオールとして存在するシステインを有する抗体フラグメントの大腸菌での発現、2官能性F(ab’)2抗体の産生のための使用 |
| US5428139A (en) | 1991-12-10 | 1995-06-27 | The Dow Chemical Company | Bicyclopolyazamacrocyclophosphonic acid complexes for use as radiopharmaceuticals |
| US5480990A (en) | 1991-12-10 | 1996-01-02 | The Dow Chemical Company | Bicyclopolyazamacrocyclocarboxylic acid complexes for use as contrast agents |
| US5739294A (en) | 1991-12-10 | 1998-04-14 | The Dow Chemical Company | Bicyclopol yazamacrocyclophosphonic acid complexes for use as contrast agents |
| ZA932522B (en) | 1992-04-10 | 1993-12-20 | Res Dev Foundation | Immunotoxins directed against c-erbB-2(HER/neu) related surface antigens |
| NZ258392A (en) | 1992-11-13 | 1997-09-22 | Idec Pharma Corp | Chimeric and radiolabelled antibodies to the b lymphocyte cellsurface antigen bp35 (cd-20) and their use in the treatment of b cell lymphona |
| US5385893A (en) | 1993-05-06 | 1995-01-31 | The Dow Chemical Company | Tricyclopolyazamacrocyclophosphonic acids, complexes and derivatives thereof, for use as contrast agents |
| US5462725A (en) | 1993-05-06 | 1995-10-31 | The Dow Chemical Company | 2-pyridylmethylenepolyazamacrocyclophosphonic acids, complexes and derivatives thereof, for use as contrast agents |
| US5789199A (en) | 1994-11-03 | 1998-08-04 | Genentech, Inc. | Process for bacterial production of polypeptides |
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| US5834456A (en) | 1996-02-23 | 1998-11-10 | The Dow Chemical Company | Polyazamacrocyclofluoromonoalkylphosphonic acids, and their complexes, for use as contrast agents |
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| NZ333346A (en) | 1996-06-28 | 2000-03-27 | Caliper Techn Corp | High-throughput screening assay systems in microscale fluidic devices |
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| EP1212453A4 (en) | 1999-09-03 | 2003-02-26 | Univ Yale | GENE FUNCTION IDENTIFICATION TECHNIQUE USING SMALL MOLECULAR PROBES |
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| US20100111856A1 (en) | 2004-09-23 | 2010-05-06 | Herman Gill | Zirconium-radiolabeled, cysteine engineered antibody conjugates |
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| PL2032606T3 (pl) | 2006-05-30 | 2014-04-30 | Genentech Inc | Przeciwciała i immunokoniugaty oraz ich zastosowania |
| EP2391714B2 (en) | 2009-01-30 | 2019-07-24 | Whitehead Institute for Biomedical Research | Methods for ligation and uses thereof |
| US8648176B2 (en) | 2009-02-27 | 2014-02-11 | Massachusetts Institute Of Technology | Engineered proteins with high affinity for DOTA chelates |
| WO2012145522A2 (en) | 2011-04-19 | 2012-10-26 | Georgia Tech Research Corporation | Deep eutectic solvent systems and methods |
| AU2012275390A1 (en) | 2011-06-28 | 2014-01-16 | Whitehead Institute For Biomedical Research | Using sortases to install click chemistry handles for protein ligation |
| EP2836638A1 (en) | 2012-04-12 | 2015-02-18 | Technische Universiteit Eindhoven | Pretreatment of lignocellulosic biomass and recovery of substituents using natural deep eutectic solvents/compound mixtures with low transition temperatures |
| CA2874436A1 (en) | 2012-05-21 | 2013-11-28 | Massachusetts Institute Of Technology | Translocation of non-natural chemical entities through anthrax protective antigen pore |
| US20140030697A1 (en) | 2012-06-14 | 2014-01-30 | Massachusetts Institute Of Technology | Sortase-mediated modification of viral surface proteins |
| US9267127B2 (en) | 2012-06-21 | 2016-02-23 | President And Fellows Of Harvard College | Evolution of bond-forming enzymes |
| MX354862B (es) | 2012-06-27 | 2018-03-23 | Hoffmann La Roche | Método para la producción de entidades dirigidas altamente selectivas hechas a la medida y biespecíficas que contienen dos entidades de unión diferentes. |
| EP2867254B1 (en) | 2012-06-27 | 2017-10-25 | F. Hoffmann-La Roche AG | Method for making antibody fc-region conjugates comprising at least one binding entity that specifically binds to a target and uses thereof |
| BR112015003938A2 (pt) * | 2012-09-14 | 2018-09-04 | Hoffmann La Roche | métodos de produção de polipeptídeos, de produção de aglutinantes multiespecíficos, de seleção de aglutinantes multiespecíficos, de seleção de anticorpos biespecíficos e de determinação de combinação, anticorpo biespecífico, formulação farmcêutica e uso de anticorpo biespecífico |
| WO2014055936A1 (en) | 2012-10-04 | 2014-04-10 | Integenx Inc. | Preservation of biological materials in non-aqueous fluid media |
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| WO2014145441A1 (en) | 2013-03-15 | 2014-09-18 | The Trustees Of The University Of Pennsylvania | Sortase-mediated protein purification and ligation |
| EP2777714A1 (en) | 2013-03-15 | 2014-09-17 | NBE-Therapeutics LLC | Method of producing an immunoligand/payload conjugate by means of a sequence-specific transpeptidase enzyme |
| AU2014258109B2 (en) | 2013-04-25 | 2016-09-29 | Abtlas Co., Ltd. | Method for refining protein including self-cutting cassette and use thereof |
| GB2529356B (en) | 2013-04-28 | 2020-12-23 | Genequantum Healthcare Co Ltd | Novel linkers, coupling intermediates, conjugates, preparation method and application thereof |
| EP3546485A1 (en) | 2013-05-10 | 2019-10-02 | Whitehead Institute for Biomedical Research | In vitro production of red blood cells with sortaggable proteins |
| AU2014262469B2 (en) | 2013-05-10 | 2019-11-14 | Whitehead Institute For Biomedical Research | Protein modification of living cells using sortase |
| CN103709230B (zh) * | 2013-12-31 | 2016-05-18 | 浙江工业大学 | 一种三肽类低共熔离子液体及其制备方法与应用 |
| JP6800154B2 (ja) | 2014-12-17 | 2020-12-16 | エフ.ホフマン−ラ ロシュ アーゲーF. Hoffmann−La Roche Aktiengesellschaft | 結合形成酵素の活性アッセイ |
-
2016
- 2016-09-22 CN CN201680055948.4A patent/CN108026560A/zh active Pending
- 2016-09-22 JP JP2018515654A patent/JP6998863B2/ja not_active Expired - Fee Related
- 2016-09-22 WO PCT/EP2016/072510 patent/WO2017050872A1/en not_active Ceased
- 2016-09-22 EP EP16775565.1A patent/EP3353314A1/en not_active Withdrawn
-
2018
- 2018-03-23 US US15/933,779 patent/US11174502B2/en not_active Expired - Fee Related
-
2021
- 2021-10-21 JP JP2021172120A patent/JP2022025094A/ja active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20090117628A1 (en) | 2007-09-21 | 2009-05-07 | Gorke Johnathan T | Enzymatic processing in deep eutectic solvents |
| WO2013016653A1 (en) | 2011-07-28 | 2013-01-31 | Cell Signaling Technology, Inc. | Multi component detection |
Non-Patent Citations (2)
| Title |
|---|
| Eur. J. Org. Chem. 2013, 4223-4228 |
| J Mol Catal B Enzym. 2011 72(3-4):163-167 |
Also Published As
| Publication number | Publication date |
|---|---|
| US20190040436A1 (en) | 2019-02-07 |
| JP2022025094A (ja) | 2022-02-09 |
| JP2018531009A (ja) | 2018-10-25 |
| EP3353314A1 (en) | 2018-08-01 |
| WO2017050872A1 (en) | 2017-03-30 |
| US11174502B2 (en) | 2021-11-16 |
| CN108026560A (zh) | 2018-05-11 |
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