JP6961256B2 - 弾性波による分離 - Google Patents
弾性波による分離 Download PDFInfo
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- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/08—Geometry, shape and general structure
- B01L2300/0809—Geometry, shape and general structure rectangular shaped
- B01L2300/0816—Cards, e.g. flat sample carriers usually with flow in two horizontal directions
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- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
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- B01L2400/04—Moving fluids with specific forces or mechanical means
- B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
- B01L2400/0433—Moving fluids with specific forces or mechanical means specific forces vibrational forces
- B01L2400/0436—Moving fluids with specific forces or mechanical means specific forces vibrational forces acoustic forces, e.g. surface acoustic waves [SAW]
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- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
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Description
本出願は、ワイツ(Weitz)らにより2015年8月27日に出願された米国仮特許出願第62/210,899号、発明の名称“弾性波による分離(又は弾性波を用いた分離:Acoustic Wave Sorting)”、に基づく優先権を主張し、その全体を参照して本明細書に組み込む。
本発明は、米国国防高等研究計画局(DARPA)(国防総省(Department of Defense))により与えられた許可番号HR0011−11−C−0093に従った政府援助でなされた。政府は本発明において一定の権利を有する。
本発明は概して表面弾性波(surface acoustic waves)などの弾性波を用いた種(species)の搬送(又は操作:manipulation)に関する。
本発明は概して、表面弾性波などの弾性波を用いた種の搬送に関する。いくつかの態様では、マイクロ流体のチャネルなどのチャネルは、2つ以上の出口と、どの出口に種が向けられる(directed)か決定するようにチャネル内の種に適用される弾性波とを有して提供され得る。例えば、表面弾性波は、細胞又は粒子などの種に対して、チャネルから、それを異なる出口に向ける溝又は他の部分に、それを偏向させるように、適用されてもよい。驚くべきことに、いくつかのケースでは、種のこの偏向は、チャネル上の入射弾性波とは異なる方向にあってもよい。本発明の他の実施形態は概してそのようなシステムを含むキット、そのようなシステムを生み出す技術等を対象とする。
本明細書の開示内容は、以下の態様を含み得る。
(態様1)
第1の表面に規定される溝を有するマイクロ流体のチャネルであって、前記溝は哺乳類の細胞が前記溝内に収まることが可能なサイズの断面寸法を有する、マイクロ流体のチャネルと、
前記マイクロ流体のチャネルの第2の表面に近接して配置された弾性波発生器であって、前記第2の表面が前記第1の表面に隣接している、弾性波発生器と、を含む装置。
(態様2)
前記マイクロ流体のチャネルは入口及び第1の出口を含み、
前記溝は種を第2の出口に向ける、態様1に記載の装置。
(態様3)
前記溝は前記マイクロ流体のチャネルに対してゼロではない角度で配置されている、態様1又は2に記載の装置。
(態様4)
前記溝は前記マイクロ流体のチャネルの前記第1の表面に平行又は垂直ではない少なくとも1つの壁により規定されている、態様1〜3のいずれか1つに記載の装置。
(態様5)
前記溝は少なくとも約20マイクロメートルの断面寸法を有する、態様1〜4のいずれか1つに記載の装置。
(態様6)
前記溝は少なくとも約25マイクロメートルの断面寸法を有する、態様1〜5のいずれか1つに記載の装置。
(態様7)
前記溝は少なくとも約30マイクロメートルの断面寸法を有する、態様1〜6のいずれか1つに記載の装置。
(態様8)
前記溝は、前記溝に隣接した前記マイクロ流体のチャネルの断面寸法よりも小さい断面寸法を有する、態様1〜7のいずれか1つに記載の装置。
(態様9)
前記弾性波発生器は、1つ以上の櫛形トランスデューサーを含む、態様1〜8のいずれか1つに記載の装置。
(態様10)
前記1つ以上の櫛形トランスデューサーの少なくとも1つは、約20マイクロメートル〜約30マイクロメートルの間隔をあけたフィンガー電極を有する、態様9に記載の装置。
(態様11)
前記1つ以上の櫛形トランスデューサーの少なくとも1つは傾斜櫛形トランスデューサーである、態様9又は10に記載の装置。
(態様12)
前記1つ以上の櫛形トランスデューサーの少なくとも1つは、互いに組み合わされた第1の電極及び第2の電極を含む、態様9〜11のいずれか1つに記載の装置。
(態様13)
前記マイクロ流体のチャネルは圧電基板中に規定される、態様1〜12のいずれか1つに記載の装置。
(態様14)
前記圧電基板はLiNbO3を含む、態様13に記載の装置。
(態様15)
前記マイクロ流体のチャネルはポリマー中に規定される、態様1〜14のいずれか1つに記載の装置。
(態様16)
前記ポリマーはポリジメチルシロキサンを含む、態様15に記載の装置。
(態様17)
前記マイクロ流体のチャネルは約1mm未満の断面寸法を有する、態様1〜16のいずれか1つに記載の装置。
(態様18)
第1の表面に規定される溝を有するマイクロ流体のチャネルであって、前記溝は少なくとも約30マイクロメートルの断面寸法を有する、マイクロ流体のチャネルと、
前記マイクロ流体の第2の表面に近接して配置された弾性波発生器であって、前記第2の表面が前記第1の表面に隣接している、弾性波発生器と、を含む装置。
(態様19)
第1の出口及び第2の出口を有するマイクロ流体のチャネル内に、流体中に含まれる種を流す工程と、
前記種を偏向させて前記第2の出口に入るように弾性波を適用する工程であって、弾性波がないときには前記種は前記第1の出口に入る、適用する工程と、を含み、
前記弾性波発生器からの前記弾性波の伝播により規定される軸方向成分と、それと実質的に垂直に規定される横方向成分と、を有する方向に、前記マイクロ流体のチャネル内で前記種を偏向させるように、弾性波発生器を用いて前記弾性波が適用され、前記横方向成分は前記軸方向成分よりも大きい、分離方法。
(態様20)
前記種は粒子である、態様19に記載の方法。
(態様21)
前記種は細胞である、態様19に記載の方法。
(態様22)
前記横方向成分は前記軸方向成分よりも少なくとも2倍大きい、態様19〜21のいずれか1つに記載の方法。
(態様23)
前記横方向成分は前記軸方向成分よりも少なくとも3倍大きい、態様19〜22のいずれか1つに記載の方法。
(態様24)
前記横方向成分は前記軸方向成分よりも少なくとも10倍大きい、態様19〜23のいずれか1つに記載の方法。
(態様25)
前記横方向成分は前記軸方向成分よりも少なくとも30倍大きい、態様19〜24のいずれか1つに記載の方法。
(態様26)
前記横方向成分は前記軸方向成分よりも少なくとも100倍大きい、態様19〜25のいずれか1つに記載の方法。
(態様27)
前記マイクロ流体のチャネルは流体を前記第2の出口に向ける溝を有する、態様19〜26のいずれか1つに記載の方法。
(態様28)
前記溝は少なくとも約20マイクロメートルの断面寸法を有する、態様19〜27のいずれか1つに記載の方法。
(態様29)
前記溝は少なくとも約25マイクロメートルの断面寸法を有する、態様19〜28のいずれか1つに記載の方法。
(態様30)
前記溝は少なくとも約30マイクロメートルの断面寸法を有する、態様19〜29のいずれか1つに記載の方法。
(態様31)
前記溝は、前記溝に隣接した前記マイクロ流体のチャネルの平行な断面寸法よりも小さい断面寸法を有する、態様19〜30のいずれか1つに記載の方法。
(態様32)
前記種は約1mm以下の平均直径を有する、態様19〜31のいずれか1つに記載の方法。
(態様33)
前記種は約100マイクロメートル以下の平均直径を有する、態様19〜32のいずれか1つに記載の方法。
(態様34)
前記種は約30マイクロメートル以下の平均直径を有する、態様19〜33のいずれか1つに記載の方法。
(態様35)
前記種は約25マイクロメートル以下の平均直径を有する、態様19〜34のいずれか1つに記載の方法。
(態様36)
前記流体は水性である、態様19〜35のいずれか1つに記載の方法。
(態様37)
前記弾性波は表面弾性波である、態様19〜36のいずれか1つに記載の方法。
(態様38)
前記弾性波は少なくとも約3dBmの電力を有する、態様19〜37のいずれか1つに記載の方法。
(態様39)
前記弾性波は少なくとも約6dBmの電力を有する、態様19〜38のいずれか1つに記載の方法。
(態様40)
前記弾性波は少なくとも約15dBmの電力を有する、態様19〜39のいずれか1つに記載の方法。
(態様41)
前記弾性波は約160MHz〜約430MHzの平均周波数を有する、態様19〜40のいずれか1つに記載の方法。
(態様42)
前記弾性波は約140MHz〜約150MHzの平均周波数を有する、態様19〜41のいずれか1つに記載の方法。
(態様43)
前記弾性波は前記マイクロ流体のチャネル内の前記種の流れ方向に実質的に垂直に適用される、態様19〜42のいずれか1つに記載の方法。
(態様44)
前記弾性波発生器は1つ以上の櫛形トランスデューサーを含む、態様19〜43のいずれか1つに記載の方法。
Claims (25)
- 第1の出口及び第2の出口を有するマイクロ流体のチャネル内に、流体中に含まれる種を流す工程であって、前記マイクロ流体のチャネルは流体を前記第2の出口に向ける溝を有し、前記溝は前記マイクロ流体のチャネル内の流体流れ方向に対して傾斜している、流す工程と、
前記種が偏向して前記溝に入って前記第2の出口に向くように、弾性波を適用する工程であって、前記弾性波がないときには前記種は前記第1の出口に入る、適用する工程と、を含み、
弾性波発生器からの前記弾性波の伝播により規定される軸方向成分と、それと実質的に垂直に規定される横方向成分と、を有する方向に、前記マイクロ流体のチャネル内で前記種を偏向させるように、前記弾性波発生器を用いて前記弾性波が適用され、前記横方向成分は前記軸方向成分よりも大きい、分離方法。 - 前記種は粒子である、請求項1に記載の方法。
- 前記種は細胞である、請求項1に記載の方法。
- 前記横方向成分は前記軸方向成分よりも少なくとも2倍大きい、請求項1〜3のいずれか1つに記載の方法。
- 前記横方向成分は前記軸方向成分よりも少なくとも3倍大きい、請求項1〜4のいずれか1つに記載の方法。
- 前記横方向成分は前記軸方向成分よりも少なくとも10倍大きい、請求項1〜5のいずれか1つに記載の方法。
- 前記横方向成分は前記軸方向成分よりも少なくとも30倍大きい、請求項1〜6のいずれか1つに記載の方法。
- 前記横方向成分は前記軸方向成分よりも少なくとも100倍大きい、請求項1〜7のいずれか1つに記載の方法。
- 前記溝は少なくとも約20マイクロメートルの断面寸法を有する、請求項1〜8のいずれか1つに記載の方法。
- 前記溝は少なくとも約25マイクロメートルの断面寸法を有する、請求項1〜9のいずれか1つに記載の方法。
- 前記溝は少なくとも約30マイクロメートルの断面寸法を有する、請求項1〜10のいずれか1つに記載の方法。
- 前記溝は、前記溝に隣接した前記マイクロ流体のチャネルの平行な断面寸法よりも小さい断面寸法を有する、請求項1〜11のいずれか1つに記載の方法。
- 前記種は約1mm以下の平均直径を有する、請求項1〜12のいずれか1つに記載の方法。
- 前記種は約100マイクロメートル以下の平均直径を有する、請求項1〜13のいずれか1つに記載の方法。
- 前記種は約30マイクロメートル以下の平均直径を有する、請求項1〜14のいずれか1つに記載の方法。
- 前記種は約25マイクロメートル以下の平均直径を有する、請求項1〜15のいずれか1つに記載の方法。
- 前記流体は水性である、請求項1〜16のいずれか1つに記載の方法。
- 前記弾性波は表面弾性波である、請求項1〜17のいずれか1つに記載の方法。
- 前記弾性波は少なくとも約3dBmの電力を有する、請求項1〜18のいずれか1つに記載の方法。
- 前記弾性波は少なくとも約6dBmの電力を有する、請求項1〜19のいずれか1つに記載の方法。
- 前記弾性波は少なくとも約15dBmの電力を有する、請求項1〜20のいずれか1つに記載の方法。
- 前記弾性波は約160MHz〜約430MHzの平均周波数を有する、請求項1〜21のいずれか1つに記載の方法。
- 前記弾性波は約140MHz〜約150MHzの平均周波数を有する、請求項1〜22のいずれか1つに記載の方法。
- 前記弾性波は前記マイクロ流体のチャネル内の前記種の流れ方向に実質的に垂直に適用される、請求項1〜23のいずれか1つに記載の方法。
- 前記弾性波発生器は1つ以上の櫛形トランスデューサーを含む、請求項1〜24のいずれか1つに記載の方法。
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CN108472621B (zh) | 2022-04-29 |
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JP6657379B2 (ja) | 2020-03-04 |
US20180257076A1 (en) | 2018-09-13 |
EP3341116A1 (en) | 2018-07-04 |
CN108472621A (zh) | 2018-08-31 |
US11559806B2 (en) | 2023-01-24 |
WO2017035287A1 (en) | 2017-03-02 |
CA3004347A1 (en) | 2017-03-02 |
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EP3341116B1 (en) | 2022-03-16 |
AU2016311341A1 (en) | 2018-03-29 |
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