JP6957469B2 - 改良された特性を有するω−ヒドロキシラーゼ関連融合ポリペプチドバリアント - Google Patents

改良された特性を有するω−ヒドロキシラーゼ関連融合ポリペプチドバリアント Download PDF

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JP6957469B2
JP6957469B2 JP2018531169A JP2018531169A JP6957469B2 JP 6957469 B2 JP6957469 B2 JP 6957469B2 JP 2018531169 A JP2018531169 A JP 2018531169A JP 2018531169 A JP2018531169 A JP 2018531169A JP 6957469 B2 JP6957469 B2 JP 6957469B2
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seq
cyp153a
amino acid
positions
fusion polypeptide
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JP2018537111A (ja
JP2018537111A5 (enExample
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アンドレアス シャーマー
バオロン シュ
エマニュエラ ポポヴァ
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ジェノマティカ， インコーポレイテッド
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0012—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7)
    • C12N9/0036—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on NADH or NADPH (1.6)
    • C12N9/0038—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on NADH or NADPH (1.6) with a heme protein as acceptor (1.6.2)
    • C12N9/0042—NADPH-cytochrome P450 reductase (1.6.2.4)
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    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
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    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/96—Stabilising an enzyme by forming an adduct or a composition; Forming enzyme conjugates
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    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6409—Fatty acids
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    • C12Y106/00—Oxidoreductases acting on NADH or NADPH (1.6)
    • C12Y106/02—Oxidoreductases acting on NADH or NADPH (1.6) with a heme protein as acceptor (1.6.2)
    • C12Y106/02004—NADPH-hemoprotein reductase (1.6.2.4), i.e. NADP-cytochrome P450-reductase
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    • C12Y301/00—Hydrolases acting on ester bonds (3.1)
    • C12Y301/01—Carboxylic ester hydrolases (3.1.1)
    • C12Y301/01005—Lysophospholipase (3.1.1.5)
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    • C12Y301/00—Hydrolases acting on ester bonds (3.1)
    • C12Y301/02—Thioester hydrolases (3.1.2)
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    • C12Y—ENZYMES
    • C12Y301/00—Hydrolases acting on ester bonds (3.1)
    • C12Y301/02—Thioester hydrolases (3.1.2)
    • C12Y301/02014—Oleoyl-[acyl-carrier-protein] hydrolase (3.1.2.14), i.e. ACP-thioesterase
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/18—Carboxylic ester hydrolases (3.1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12Y101/00—Oxidoreductases acting on the CH-OH group of donors (1.1)
    • C12Y101/01—Oxidoreductases acting on the CH-OH group of donors (1.1) with NAD+ or NADP+ as acceptor (1.1.1)

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  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
JP2018531169A 2015-12-15 2016-12-13 改良された特性を有するω−ヒドロキシラーゼ関連融合ポリペプチドバリアント Active JP6957469B2 (ja)

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JP2021164702A JP7458358B2 (ja) 2015-12-15 2021-10-06 改良された特性を有するω－ヒドロキシラーゼ関連融合ポリペプチドバリアント

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PCT/EP2015/079832 WO2017101987A1 (en) 2015-12-15 2015-12-15 Omega-hydroxylase-related fusion polypeptide variants with improved properties
EPPCT/EP2015/079832 2015-12-15
PCT/US2016/066405 WO2017106205A1 (en) 2015-12-15 2016-12-13 Omega-hydroxylase-related fusion polypeptide variants with improved properties

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JP2021164702A Active JP7458358B2 (ja) 2015-12-15 2021-10-06 改良された特性を有するω－ヒドロキシラーゼ関連融合ポリペプチドバリアント

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AU (1) AU2016370490B2 (enExample)
CA (1) CA3008836A1 (enExample)
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WO2017101987A1 (en) 2015-12-15 2017-06-22 REG Life Sciences, LLC Omega-hydroxylase-related fusion polypeptide variants with improved properties
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KR102516485B1 (ko) 2018-10-31 2023-04-03 삼성전자주식회사 폴더블 하우징을 포함하는 전자 장치

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WO2017101987A1 (en) * 2015-12-15 2017-06-22 REG Life Sciences, LLC Omega-hydroxylase-related fusion polypeptide variants with improved properties

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WO2017106205A1 (en) 2017-06-22
AU2016370490B2 (en) 2023-01-05
CN108699536B (zh) 2022-08-02
ES2908400T3 (es) 2022-04-29
CN116334012A (zh) 2023-06-27
EP3390620B1 (en) 2022-01-05
CN108699536A (zh) 2018-10-23
CA3008836A1 (en) 2017-06-22
KR20250117480A (ko) 2025-08-04
AU2016370490A1 (en) 2018-06-21
US20210230562A1 (en) 2021-07-29
KR20180132604A (ko) 2018-12-12
JP2018537111A (ja) 2018-12-20
JP7458358B2 (ja) 2024-03-29
JP2022002535A (ja) 2022-01-11
MY196584A (en) 2023-04-19
SI3390620T1 (sl) 2022-05-31
US12180515B2 (en) 2024-12-31
CN116334013A (zh) 2023-06-27
EP4026899A1 (en) 2022-07-13
EP3390620A1 (en) 2018-10-24
MX2018007298A (es) 2019-01-10
US20230159901A1 (en) 2023-05-25
WO2017101987A1 (en) 2017-06-22
KR102840377B1 (ko) 2025-08-01
US10787648B2 (en) 2020-09-29
BR112018012193A2 (pt) 2018-12-04
MY194277A (en) 2022-11-25
US11384341B2 (en) 2022-07-12
US20190002845A1 (en) 2019-01-03

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