CA3008836A1 - Omega-hydroxylase-related fusion polypeptide variants with improved properties - Google Patents

Omega-hydroxylase-related fusion polypeptide variants with improved properties Download PDF

Info

Publication number
CA3008836A1
CA3008836A1 CA3008836A CA3008836A CA3008836A1 CA 3008836 A1 CA3008836 A1 CA 3008836A1 CA 3008836 A CA3008836 A CA 3008836A CA 3008836 A CA3008836 A CA 3008836A CA 3008836 A1 CA3008836 A1 CA 3008836A1
Authority
CA
Canada
Prior art keywords
seq
cyp153a
fusion polypeptide
amino acid
fatty acid
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CA3008836A
Other languages
English (en)
French (fr)
Inventor
Andreas Schirmer
Baolong Zhu
Emanuela POPOVA
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Genomatica Inc
Original Assignee
REG Life Sciences LLC
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by REG Life Sciences LLC filed Critical REG Life Sciences LLC
Publication of CA3008836A1 publication Critical patent/CA3008836A1/en
Pending legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0012—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7)
    • C12N9/0036—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on NADH or NADPH (1.6)
    • C12N9/0038—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on NADH or NADPH (1.6) with a heme protein as acceptor (1.6.2)
    • C12N9/0042—NADPH-cytochrome P450 reductase (1.6.2.4)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/96—Stabilising an enzyme by forming an adduct or a composition; Forming enzyme conjugates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6409—Fatty acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y106/00—Oxidoreductases acting on NADH or NADPH (1.6)
    • C12Y106/02—Oxidoreductases acting on NADH or NADPH (1.6) with a heme protein as acceptor (1.6.2)
    • C12Y106/02004—NADPH-hemoprotein reductase (1.6.2.4), i.e. NADP-cytochrome P450-reductase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y301/00—Hydrolases acting on ester bonds (3.1)
    • C12Y301/01—Carboxylic ester hydrolases (3.1.1)
    • C12Y301/01005—Lysophospholipase (3.1.1.5)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y301/00—Hydrolases acting on ester bonds (3.1)
    • C12Y301/02—Thioester hydrolases (3.1.2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y301/00—Hydrolases acting on ester bonds (3.1)
    • C12Y301/02—Thioester hydrolases (3.1.2)
    • C12Y301/02014—Oleoyl-[acyl-carrier-protein] hydrolase (3.1.2.14), i.e. ACP-thioesterase
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/18—Carboxylic ester hydrolases (3.1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y101/00—Oxidoreductases acting on the CH-OH group of donors (1.1)
    • C12Y101/01—Oxidoreductases acting on the CH-OH group of donors (1.1) with NAD+ or NADP+ as acceptor (1.1.1)

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Mycology (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
CA3008836A 2015-12-15 2016-12-13 Omega-hydroxylase-related fusion polypeptide variants with improved properties Pending CA3008836A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EPPCT/EP2015/079832 2015-12-15
PCT/EP2015/079832 WO2017101987A1 (en) 2015-12-15 2015-12-15 Omega-hydroxylase-related fusion polypeptide variants with improved properties
PCT/US2016/066405 WO2017106205A1 (en) 2015-12-15 2016-12-13 Omega-hydroxylase-related fusion polypeptide variants with improved properties

Publications (1)

Publication Number Publication Date
CA3008836A1 true CA3008836A1 (en) 2017-06-22

Family

ID=55022448

Family Applications (1)

Application Number Title Priority Date Filing Date
CA3008836A Pending CA3008836A1 (en) 2015-12-15 2016-12-13 Omega-hydroxylase-related fusion polypeptide variants with improved properties

Country Status (12)

Country Link
US (3) US10787648B2 (enExample)
EP (2) EP3390620B1 (enExample)
JP (2) JP6957469B2 (enExample)
KR (2) KR102840377B1 (enExample)
CN (3) CN116334013A (enExample)
AU (1) AU2016370490B2 (enExample)
CA (1) CA3008836A1 (enExample)
ES (1) ES2908400T3 (enExample)
MX (1) MX2018007298A (enExample)
MY (2) MY194277A (enExample)
SI (1) SI3390620T1 (enExample)
WO (2) WO2017101987A1 (enExample)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2014277874B2 (en) 2013-06-14 2020-12-03 Genomatica, Inc. Methods of producing omega-hydroxylated fatty acid derivatives
MX372803B (es) * 2014-06-16 2020-07-03 Genomatica Inc Polipeptidos de fusion relacionados con omega-hidroxilasa con propiedades mejoradas.
WO2017101987A1 (en) * 2015-12-15 2017-06-22 REG Life Sciences, LLC Omega-hydroxylase-related fusion polypeptide variants with improved properties
US20210189439A1 (en) * 2018-05-10 2021-06-24 Genomatica, Inc. Multifunctional Fatty Acid Derivatives And Biosynthesis Thereof
KR102516485B1 (ko) 2018-10-31 2023-04-03 삼성전자주식회사 폴더블 하우징을 포함하는 전자 장치

Family Cites Families (58)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA1305083C (en) 1987-02-18 1992-07-14 Yoshiharu Kimura Microorganism belonging to genus rhodococcus, and a process for producing alkene derivative and unsaturated fatty acid
US5482846A (en) 1988-08-31 1996-01-09 University Of Florida Ethanol production in Gram-positive microbes
US5028539A (en) 1988-08-31 1991-07-02 The University Of Florida Ethanol production using engineered mutant E. coli
US5000000A (en) 1988-08-31 1991-03-19 University Of Florida Ethanol production by Escherichia coli strains co-expressing Zymomonas PDC and ADH genes
US5424202A (en) 1988-08-31 1995-06-13 The University Of Florida Ethanol production by recombinant hosts
EP0528881A4 (en) 1990-04-24 1993-05-26 Stratagene Methods for phenotype creation from multiple gene populations
US5602030A (en) 1994-03-28 1997-02-11 University Of Florida Research Foundation Recombinant glucose uptake system
US6428767B1 (en) 1995-05-12 2002-08-06 E. I. Du Pont De Nemours And Company Method for identifying the source of carbon in 1,3-propanediol
US5939250A (en) 1995-12-07 1999-08-17 Diversa Corporation Production of enzymes having desired activities by mutagenesis
US5965408A (en) 1996-07-09 1999-10-12 Diversa Corporation Method of DNA reassembly by interrupting synthesis
CN100379716C (zh) 2002-04-29 2008-04-09 陶氏环球技术公司 关于种子油工业应用的综合化学方法
EP1811029A4 (en) 2004-11-10 2008-03-12 Marine Biotech Inst Co Ltd PROCESS FOR ISOLATING THE P450 GENE
AU2007254151A1 (en) 2006-05-19 2007-11-29 Ls9, Inc. Production of fatty acids and derivatives thereof
US20100242345A1 (en) 2006-05-19 2010-09-30 LS9, Inc Production of fatty acids & derivatives thereof
US8110670B2 (en) 2006-05-19 2012-02-07 Ls9, Inc. Enhanced production of fatty acid derivatives
CN101558027B (zh) 2006-10-13 2013-10-16 埃莱文斯可更新科学公司 通过复分解反应制备α，ω-二羧酸烯烃衍生物的方法
US8501973B2 (en) 2006-10-13 2013-08-06 Elevance Renewable Sciences, Inc. Synthesis of terminal alkenes from internal alkenes via olefin metathesis
US8110093B2 (en) 2007-03-14 2012-02-07 Ls9, Inc. Process for producing low molecular weight hydrocarbons from renewable resources
CA3035878C (en) 2007-03-28 2023-01-03 REG Life Sciences, LLC Enhanced production of fatty acid derivatives
US20080293060A1 (en) 2007-04-23 2008-11-27 Ls9, Inc. Methods and Compositions for Identification of Hydrocarbon Response, Transport and Biosynthesis Genes
JP5912251B2 (ja) 2007-05-22 2016-06-01 アールイージー ライフ サイエンシズ リミテッド ライアビリティ カンパニー 炭化水素産生遺伝子およびその使用方法
JP5324129B2 (ja) 2007-05-25 2013-10-23 神戸天然物化学株式会社 Ｃｙｐ１５３による芳香族化合物の製造方法
WO2009009391A2 (en) 2007-07-06 2009-01-15 Ls9, Inc. Systems and methods for the production of fatty esters
FR2921362B1 (fr) 2007-09-20 2012-09-21 Arkema France Procede de synthese d'acides gras omega-insatures
WO2009042950A1 (en) 2007-09-27 2009-04-02 Ls9, Inc. Reduction of the toxic effect of impurities from raw materials by extractive fermentation
TWI351779B (en) 2007-12-03 2011-11-01 Advance Smart Ind Ltd Apparatus and method for correcting residual capac
US8183028B2 (en) 2007-12-21 2012-05-22 Ls9, Inc. Methods and compositions for producing olefins
JP2011522525A (ja) 2008-05-16 2011-08-04 エルエス９・インコーポレイテッド 炭化水素を製造するための方法および組成物
WO2009142983A1 (en) 2008-05-23 2009-11-26 Alliant Techsystems Inc. Broadband patch antenna and antenna system
US8273694B2 (en) 2008-07-28 2012-09-25 Jeffrey A Brown Synthetic compositions obtained from algae
WO2010021711A1 (en) 2008-08-18 2010-02-25 Ls9, Inc. Systems and methods for the production of mixed fatty esters
TW201016657A (en) 2008-08-28 2010-05-01 Mitsui Chemicals Inc Olefin production process
CA3038491A1 (en) 2008-10-07 2010-04-15 REG Life Sciences, LLC Bacterial host cells engineered to express a carboxylic acid reductase and a thioesterase
WO2010062480A2 (en) 2008-10-28 2010-06-03 Ls9, Inc. Methods and compositions for producing fatty alcohols
US7989875B2 (en) 2008-11-24 2011-08-02 Nxp B.V. BiCMOS integration of multiple-times-programmable non-volatile memories
EP3564345A1 (en) 2008-12-23 2019-11-06 REG Life Sciences, LLC Methods and compositions related to thioesterase enzymes
US8071799B2 (en) 2009-01-29 2011-12-06 Energy & Environmental Research Center Foundation Chain-selective synthesis of fuel components and chemical feedstocks
CA2758298A1 (en) 2009-04-10 2010-10-14 Ls9, Inc. Production of commercial biodiesel from genetically modified microorganisms
BRPI1015313B1 (pt) 2009-04-27 2021-06-01 Genomatica, Inc. Método fermentativo para produzir um éster de ácido graxo empregando um microorganismo geneticamente modificado e método para a produção de ésteres de ácidos graxos
EP2424989A2 (en) 2009-05-01 2012-03-07 The Regents of The University of California Product of fatty acid esters from biomass polymers
CN102656273B (zh) 2009-09-25 2016-05-25 Reg生命科学有限责任公司 脂肪酸衍生物的产生
US8809563B2 (en) 2009-11-09 2014-08-19 Exxonmobil Chemical Patents Inc. Metathesis catalyst and process for use thereof
US8237003B2 (en) 2009-11-09 2012-08-07 Exxonmobil Chemical Patents Inc. Metathesis catalyst and process for use thereof
US8361769B1 (en) 2009-11-16 2013-01-29 U.S. Department Of Energy Regioselective alkane hydroxylation with a mutant CYP153A6 enzyme
US20110124071A1 (en) 2009-11-17 2011-05-26 LS9, Inc Methods and compositions for producing hydrocarbons
US8530221B2 (en) 2010-01-14 2013-09-10 Ls9, Inc. Production of branched chain fatty acids and derivatives thereof in recombinant microbial cells
WO2011127409A2 (en) 2010-04-08 2011-10-13 Ls9, Inc. Methods and compositions related to fatty alcohol biosynthetic enzymes
SG185780A1 (en) 2010-05-28 2013-01-30 Solazyme Inc Tailored oils produced from recombinant heterotrophic microorganisms
US8372610B2 (en) 2010-09-15 2013-02-12 Ls9, Inc. Production of odd chain fatty acid derivatives in recombinant microbial cells
WO2012071439A1 (en) 2010-11-22 2012-05-31 The Regents Of The University Of California Host cells and methods for producing diacid compounds
CA2825691C (en) 2011-02-02 2020-08-25 Solazyme, Inc. Tailored oils produced from recombinant oleaginous microorganisms
CA2841796C (en) 2011-07-06 2021-06-29 Verdezyne, Inc. Biological methods for preparing a fatty dicarboxylic acid
DE102011110946A1 (de) 2011-08-15 2016-01-21 Evonik Degussa Gmbh Biotechnologisches Syntheseverfahren von omegafunktionalisierten Carbonsäuren und Carbonsäure-Estern aus einfachen Kohlenstoffquellen
EP2639308A1 (de) 2012-03-12 2013-09-18 Evonik Industries AG Enzymatische omega-Oxidation und -Aminierung von Fettsäuren
FR3003577B1 (fr) 2013-03-19 2016-05-06 Ferropem Inoculant a particules de surface
AU2014277874B2 (en) * 2013-06-14 2020-12-03 Genomatica, Inc. Methods of producing omega-hydroxylated fatty acid derivatives
MX372803B (es) * 2014-06-16 2020-07-03 Genomatica Inc Polipeptidos de fusion relacionados con omega-hidroxilasa con propiedades mejoradas.
WO2017101987A1 (en) * 2015-12-15 2017-06-22 REG Life Sciences, LLC Omega-hydroxylase-related fusion polypeptide variants with improved properties

Also Published As

Publication number Publication date
EP4026899A1 (en) 2022-07-13
JP6957469B2 (ja) 2021-11-02
MX2018007298A (es) 2019-01-10
EP3390620B1 (en) 2022-01-05
BR112018012193A2 (pt) 2018-12-04
ES2908400T3 (es) 2022-04-29
CN108699536A (zh) 2018-10-23
CN116334012A (zh) 2023-06-27
US10787648B2 (en) 2020-09-29
WO2017101987A1 (en) 2017-06-22
US12180515B2 (en) 2024-12-31
JP2022002535A (ja) 2022-01-11
SI3390620T1 (sl) 2022-05-31
WO2017106205A1 (en) 2017-06-22
KR20250117480A (ko) 2025-08-04
AU2016370490B2 (en) 2023-01-05
US20210230562A1 (en) 2021-07-29
JP7458358B2 (ja) 2024-03-29
US20190002845A1 (en) 2019-01-03
US20230159901A1 (en) 2023-05-25
US11384341B2 (en) 2022-07-12
KR20180132604A (ko) 2018-12-12
AU2016370490A1 (en) 2018-06-21
EP3390620A1 (en) 2018-10-24
MY196584A (en) 2023-04-19
CN116334013A (zh) 2023-06-27
MY194277A (en) 2022-11-25
KR102840377B1 (ko) 2025-08-01
CN108699536B (zh) 2022-08-02
JP2018537111A (ja) 2018-12-20

Similar Documents

Publication Publication Date Title
US11130944B2 (en) Acyl-ACP reductase with improved properties
US12180515B2 (en) Omega-hydroxylase-related fusion polypeptide variants with improved properties
US20230167419A1 (en) Omega-Hydroxylase-Related Fusion Polypeptides With Improved Properties
EP2984490A1 (en) Methods of producing omega-hydroxylated fatty acid derivatives
CA2883968A1 (en) Improved production of fatty acid derivatives

Legal Events

Date Code Title Description
EEER Examination request

Effective date: 20211208

B12 Application deemed to be withdrawn, abandoned or lapsed

Free format text: ST27 STATUS EVENT CODE: N-6-6-B10-B12-B303 (AS PROVIDED BY THE NATIONAL OFFICE); EVENT TEXT: DEEMED ABANDONED - FAILURE TO RESPOND TO AN EXAMINER'S REQUISITION

Effective date: 20240903

N11 Application terminated

Free format text: ST27 STATUS EVENT CODE: T-6-6-N10-N11-N106 (AS PROVIDED BY THE NATIONAL OFFICE); EVENT TEXT: APPLICATION NOT REINSTATED BY DEADLINE

Effective date: 20250613

W00 Other event occurred

Free format text: ST27 STATUS EVENT CODE: N-6-6-W10-W00-W100 (AS PROVIDED BY THE NATIONAL OFFICE); EVENT TEXT: LETTER SENT

Effective date: 20260105

W00 Other event occurred

Free format text: ST27 STATUS EVENT CODE: N-6-6-W10-W00-W100 (AS PROVIDED BY THE NATIONAL OFFICE); EVENT TEXT: LETTER SENT

Effective date: 20260205