JP6951354B2 - 高スループットの撮像のための方法及びデバイス - Google Patents
高スループットの撮像のための方法及びデバイス Download PDFInfo
- Publication number
- JP6951354B2 JP6951354B2 JP2018544238A JP2018544238A JP6951354B2 JP 6951354 B2 JP6951354 B2 JP 6951354B2 JP 2018544238 A JP2018544238 A JP 2018544238A JP 2018544238 A JP2018544238 A JP 2018544238A JP 6951354 B2 JP6951354 B2 JP 6951354B2
- Authority
- JP
- Japan
- Prior art keywords
- sample
- sub
- array
- sample holder
- observation
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Images
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/645—Specially adapted constructive features of fluorimeters
- G01N21/6456—Spatial resolved fluorescence measurements; Imaging
- G01N21/6458—Fluorescence microscopy
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/47—Scattering, i.e. diffuse reflection
- G01N21/4738—Diffuse reflection, e.g. also for testing fluids, fibrous materials
- G01N21/474—Details of optical heads therefor, e.g. using optical fibres
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/47—Scattering, i.e. diffuse reflection
- G01N21/49—Scattering, i.e. diffuse reflection within a body or fluid
- G01N21/51—Scattering, i.e. diffuse reflection within a body or fluid inside a container, e.g. in an ampoule
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6486—Measuring fluorescence of biological material, e.g. DNA, RNA, cells
-
- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B19/00—Condensers, e.g. light collectors or similar non-imaging optics
- G02B19/0004—Condensers, e.g. light collectors or similar non-imaging optics characterised by the optical means employed
- G02B19/0019—Condensers, e.g. light collectors or similar non-imaging optics characterised by the optical means employed having reflective surfaces only (e.g. louvre systems, systems with multiple planar reflectors)
-
- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B19/00—Condensers, e.g. light collectors or similar non-imaging optics
- G02B19/0033—Condensers, e.g. light collectors or similar non-imaging optics characterised by the use
- G02B19/0047—Condensers, e.g. light collectors or similar non-imaging optics characterised by the use for use with a light source
- G02B19/0052—Condensers, e.g. light collectors or similar non-imaging optics characterised by the use for use with a light source the light source comprising a laser diode
- G02B19/0057—Condensers, e.g. light collectors or similar non-imaging optics characterised by the use for use with a light source the light source comprising a laser diode in the form of a laser diode array, e.g. laser diode bar
-
- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/0004—Microscopes specially adapted for specific applications
- G02B21/002—Scanning microscopes
- G02B21/0024—Confocal scanning microscopes (CSOMs) or confocal "macroscopes"; Accessories which are not restricted to use with CSOMs, e.g. sample holders
- G02B21/0032—Optical details of illumination, e.g. light-sources, pinholes, beam splitters, slits, fibers
-
- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/0004—Microscopes specially adapted for specific applications
- G02B21/002—Scanning microscopes
- G02B21/0024—Confocal scanning microscopes (CSOMs) or confocal "macroscopes"; Accessories which are not restricted to use with CSOMs, e.g. sample holders
- G02B21/0036—Scanning details, e.g. scanning stages
-
- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/0004—Microscopes specially adapted for specific applications
- G02B21/002—Scanning microscopes
- G02B21/0024—Confocal scanning microscopes (CSOMs) or confocal "macroscopes"; Accessories which are not restricted to use with CSOMs, e.g. sample holders
- G02B21/0052—Optical details of the image generation
- G02B21/0064—Optical details of the image generation multi-spectral or wavelength-selective arrangements, e.g. wavelength fan-out, chromatic profiling
-
- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/0004—Microscopes specially adapted for specific applications
- G02B21/002—Scanning microscopes
- G02B21/0024—Confocal scanning microscopes (CSOMs) or confocal "macroscopes"; Accessories which are not restricted to use with CSOMs, e.g. sample holders
- G02B21/0052—Optical details of the image generation
- G02B21/0076—Optical details of the image generation arrangements using fluorescence or luminescence
-
- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/24—Base structure
- G02B21/26—Stages; Adjusting means therefor
-
- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/33—Immersion oils, or microscope systems or objectives for use with immersion fluids
-
- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B27/00—Optical systems or apparatus not provided for by any of the groups G02B1/00 - G02B26/00, G02B30/00
- G02B27/10—Beam splitting or combining systems
- G02B27/1006—Beam splitting or combining systems for splitting or combining different wavelengths
-
- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B27/00—Optical systems or apparatus not provided for by any of the groups G02B1/00 - G02B26/00, G02B30/00
- G02B27/10—Beam splitting or combining systems
- G02B27/14—Beam splitting or combining systems operating by reflection only
- G02B27/141—Beam splitting or combining systems operating by reflection only using dichroic mirrors
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N2021/6417—Spectrofluorimetric devices
- G01N2021/6419—Excitation at two or more wavelengths
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/645—Specially adapted constructive features of fluorimeters
- G01N2021/6463—Optics
- G01N2021/6471—Special filters, filter wheel
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/645—Specially adapted constructive features of fluorimeters
- G01N2021/6463—Optics
- G01N2021/6478—Special lenses
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/645—Specially adapted constructive features of fluorimeters
- G01N2021/6482—Sample cells, cuvettes
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/645—Specially adapted constructive features of fluorimeters
- G01N2021/6484—Optical fibres
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2201/00—Features of devices classified in G01N21/00
- G01N2201/08—Optical fibres; light guides
- G01N2201/0833—Fibre array at detector, resolving
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2201/00—Features of devices classified in G01N21/00
- G01N2201/10—Scanning
- G01N2201/103—Scanning by mechanical motion of stage
Landscapes
- Physics & Mathematics (AREA)
- General Physics & Mathematics (AREA)
- Chemical & Material Sciences (AREA)
- Optics & Photonics (AREA)
- Analytical Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Immunology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Pathology (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Oil, Petroleum & Natural Gas (AREA)
- Spectroscopy & Molecular Physics (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
- Microscoopes, Condenser (AREA)
- Investigating Or Analysing Materials By Optical Means (AREA)
Description
前記試料を、線焦点又は焦点の配列で同時に照明するための照明手段と、
試料から放出され又は散乱された光子を、視野の配列の中で同時に検出するための検出手段と、を備え、
ここで撮像中の光子が放出されるか又は散乱される試料中のサブ観察体積(sub-observation volumes)の配列は、照明手段からの線焦点又は焦点の配列が検出手段の対応する視野の配列と重なる空間における体積によって規定され、
前記装置は、前記試料をその面に保持するように構成された試料ホルダを備え、前記試料ホルダを回転させることによって、前記試料の少なくとも一部が前記サブ観察体積の少なくとも1つを通って搬送されるように、前記試料ホルダは回転可能に配置されることを特徴とする。
a)回転可能に配置された試料ホルダの面に撮像される試料を提供することと
b)撮像中に光子が放出又は散乱される前記試料内のサブ観察体積の配列が照明手段からの線焦点又は焦点の配列が前記検出手段の視野の対応する配列と重なる空間の前記体積によって規定されるように前記試料を線焦点又は焦点の配列で同時に照明し、視野の配列内で同時に前記試料から放出又は散乱した光子を検出することと、
c)撮像中に試料ホルダを回転させることによって、前記サブ観察体積の少なくとも1つを通して前記試料の少なくとも一部を搬送すること、を含んでいる。
Claims (31)
- 試料を撮像するための装置であって、前記装置は、
前記試料を、線焦点又は焦点の配列で同時に照明するための照明手段と、
試料から放出され又は散乱された光子を、視野の配列の中で同時に検出するための検出手段とを備え、
ここで撮像中の光子が放出されるか又は散乱される試料中のサブ観察体積(sub-observation volumes)の配列は、照明手段からの線焦点又は焦点の配列が前記検出手段の対応する視野の配列と重なる空間における体積によって規定され、
前記装置は、前記試料をその側面に保持するように構成された円筒状試料ホルダを含み、前記円筒状試料ホルダは、前記円筒状試料ホルダを回転させることによって、前記試料の少なくとも一部が前記サブ観察体積の少なくとも1つを通して搬送されるように回転可能に配置されることを特徴とし、
前記照明手段からの前記線焦点又は焦点の配列の中心軸と、前記検出手段の視野の対応する配列が、実質的に平行であることを特徴とする、装置。 - 前記検出手段は、前記試料内の点がサブ観察体積を通過するのにかかる時間と同じ程度、又はより短い検出システム上昇時間を有する請求項1に記載の装置。
- 前記サブ観察体積の配列は、観察体積の少なくとも1つの行に配置される請求項1から2のいずれか一項に記載の装置。
- 前記サブ観察体積の少なくとも1つの行に沿って描かれた仮想線と、前記行の前記サブ観察の少なくとも1つを通って搬送される試料の運動ベクトルとの間の角度は、0より大きい請求項3に記載の装置。
- 前記サブ観察体積の配列は、観察体積の平面格子に配置される請求項1から2のいずれか一項に記載の装置。
- 前記サブ観察体積の格子の仮想平面の法線ベクトルと、前記行の前記サブ観察体積の少なくとも1つを通って搬送される試料の前記運動ベクトルとは、非直交である請求項5に記載の装置。
- 前記照明手段は、マイクロレンズ配列を備える請求項1〜6のいずれか一項に記載の装置。
- 前記検出手段がピンホール配列又は光ファイバの配列を含む請求項1から7のいずれか一項に記載の装置。
- 各サブ観察体積は、前記ピンホール配列又は光ファイバの配列の別々のピンホール又は光ファイバを介して前記検出手段によって観察されるものであり、前記ピンホール又は光ファイバは、前記照明手段の前記線焦点または焦点の配列と平行な寸法の各サブ観察体積を規定する、請求項8に記載の装置。
- 前記検出手段はガイガーモードでのアバランシェフォトダイオード検出器を含む請求項1から9のいずれか一項に記載の装置。
- 前記試料が、回転中に前記試料ホルダの回転軸からほぼ一定の半径方向距離に配置される請求項1から10のいずれか一項に記載の装置。
- 前記試料ホルダが空気軸受によって懸架される請求項1から11のいずれか一項に記載の装置。
- 前記試料と、前記サブ観察体積の配列との間の相対的、接線的、及び/又は半径方向の位置を、前記試料ホルダの回転ラップごとに少なくとも1回測定して、検出された信号を前記試料内の対応する絶対位置と相関させる手段をさらに備える請求項1から12のいずれか一項に記載の装置。
- 前記円筒状試料ホルダは、回転中に前記試料ホルダの回転軸に沿って搬送されるように、前記回転軸に沿って移動可能に配置される請求項1から13のいずれか一項に記載の装置。
- 前記円筒状試料ホルダは、試料容器ホルダと、前記試料容器ホルダに巻き付けられて取り付けられるように構成された取り外し可能な板状試料容器とを備える請求項1〜14のいずれか一項に記載の装置。
- 前記円筒状試料ホルダは、前記試料を保持するための溝、ウェル等を含む請求項1から15のいずれか一項に記載の装置。
- 前記円筒状試料ホルダは、前記試料を含有するために、前記溝、ウェル等を覆う薄いプレート又はフィルムの形態の蓋をさらに含む、請求項16に記載の装置。
- さらに、回転中に液体層を面に広げるための、前記円筒状試料ホルダの外向き面の近傍に配置されたコーティングデバイスを含む請求項1から17のいずれか一項に記載の装置。
- 前記コーティングデバイスは、顕微鏡対物レンズに取り付けられた顕微鏡スライドによって形成され、ここで前記顕微鏡スライドは、前記円筒状試料ホルダの前記外向き面の近傍に配置される請求項18に記載の装置。
- 試料を撮像する方法であって、
a)回転可能に配置された試料ホルダの面に撮像される試料を提供することであって、ここで前記試料ホルダが円筒状であり、撮像される前記試料は、前記円筒状試料ホルダの側面に配置される;
b)撮像中に光子が放出又は散乱される前記試料内のサブ観察体積の配列が、照明手段からの線焦点又は焦点の配列が検出手段の視野の対応する配列と重なる空間における前記体積によって規定されるよう、前記試料を線焦点又は焦点の配列で同時に照明し、視野の配列内で同時に前記試料から放出又は散乱した光子を検出することであって、ここで前記照明手段からの前記線焦点又は焦点の配列の中心軸と、前記検出手段の視野の対応する配列が、実質的に平行である;及び、
c)撮像中に前記試料ホルダを回転させることによって、前記サブ観察体積の少なくとも1つを通して前記試料の少なくとも一部を搬送すること、
を含む方法。 - 前記検出は、前記試料内の点がサブ観察体積を通過するのにかかる時間と同じ程度、又はより短い検出システム上昇時間を有する請求項20に記載の方法。
- 前記サブ観察体積の配列は、観察体積の少なくとも1つの行に配置される請求項20〜21のいずれか一項に記載の方法。
- 前記サブ観察体積の少なくとも1つの行に沿って描かれた仮想線と、前記行の前記サブ観察の少なくとも1つを通って搬送される試料の運動ベクトルとの間の角度は、0より大きい、請求項22に記載の方法。
- 前記サブ観察体積の配列は、観察体積の平面格子に配置される請求項20から23のいずれか一項に記載の方法。
- 前記サブ観察体積の格子の仮想平面の前記法線ベクトルと、前記行の前記サブ観察体積の少なくとも1つを通って搬送される試料の前記運動ベクトルとは、非直交である請求項24に記載の方法。
- 検出はガイガーモードでのアバランシェフォトダイオード検出器を使用して行う請求項20から25のいずれか一項に記載の方法。
- 前記試料が、回転中に前記試料ホルダの回転軸からほぼ一定の半径方向距離に配置される前記請求項20から26のいずれか一項に記載の方法。
- 前記試料は、1〜100m/sの範囲内の速度で前記サブ観察体積に対して回転させる、請求項20から27のいずれか一項に記載の方法。
- 前記試料と、前記サブ観察体積の配列との間の相対的、接線的、及び/又は半径方向の位置を、前記試料ホルダの回転ラップごとに少なくとも1回測定して、検出された信号を前記試料内の前記対応する絶対位置と相関させる手段をさらに備える請求項20から28のいずれか一項に記載の方法。
- 前記試料は、回転中、前記円筒状試料ホルダの前記回転軸に沿って搬送される請求項22から29のいずれか一項に記載の方法。
- 回転中に、前記円筒状試料ホルダの前記外向き面に液体層を広げることをさらに含む請求項22から30のいずれか一項に記載の方法。
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
SE1650251 | 2016-02-26 | ||
SE1650251-0 | 2016-02-26 | ||
PCT/EP2017/054244 WO2017144619A1 (en) | 2016-02-26 | 2017-02-23 | Method and device for high throughput imaging |
Publications (2)
Publication Number | Publication Date |
---|---|
JP2019508691A JP2019508691A (ja) | 2019-03-28 |
JP6951354B2 true JP6951354B2 (ja) | 2021-10-20 |
Family
ID=58162576
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP2018544238A Active JP6951354B2 (ja) | 2016-02-26 | 2017-02-23 | 高スループットの撮像のための方法及びデバイス |
Country Status (5)
Country | Link |
---|---|
US (1) | US10732113B2 (ja) |
EP (1) | EP3420338B1 (ja) |
JP (1) | JP6951354B2 (ja) |
CN (1) | CN108700520B (ja) |
WO (1) | WO2017144619A1 (ja) |
Families Citing this family (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
FR3098930B1 (fr) * | 2019-07-18 | 2023-04-28 | Univ Versailles Saint Quentin En Yvelines | Dispositif d’observation d’une cellule ou d’un ensemble de cellules vivantes |
DE102019129932B4 (de) * | 2019-11-06 | 2023-12-21 | Technische Universität Braunschweig | Optische Detektionseinrichtung und Verfahren zum Betreiben einer optischen Detektionseinrichtung |
CN110927948A (zh) * | 2019-12-05 | 2020-03-27 | 锘海生物科学仪器(上海)股份有限公司 | 具有固态光学系统的荧光显微镜 |
EP4053538A1 (en) | 2021-03-01 | 2022-09-07 | Single Technologies AB | Liquid handling means for performing assays |
Family Cites Families (66)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US3701620A (en) * | 1971-10-21 | 1972-10-31 | Science Spectrum | Sample scattering cell for a photometer |
JPS5039594A (ja) * | 1973-08-13 | 1975-04-11 | ||
US4902132A (en) * | 1988-06-13 | 1990-02-20 | Hipple Cancer Research Corporation | Automated capillary scanning system |
WO1990005297A1 (de) * | 1988-10-31 | 1990-05-17 | Schweizerische Eidgenossenschaft Psi Paul Scherrer Institut | Vorrichtung zur gleichzeitigen berührungslosen prüfung einer mehrzahl von stellen eines prüfguts sowie deren verwendung |
GB2273994A (en) * | 1992-12-18 | 1994-07-06 | Morphometrix Inc | Process microscopy system |
US5470710A (en) * | 1993-10-22 | 1995-11-28 | University Of Utah | Automated hybridization/imaging device for fluorescent multiplex DNA sequencing |
US5671085A (en) * | 1995-02-03 | 1997-09-23 | The Regents Of The University Of California | Method and apparatus for three-dimensional microscopy with enhanced depth resolution |
US7047064B1 (en) * | 1995-07-13 | 2006-05-16 | Lucid, Inc. | Microscopic imaging apparatus and method |
US6745067B1 (en) * | 1998-09-14 | 2004-06-01 | Lucid, Inc. | System for marking the locations of imaged tissue with respect to the surface of the tissue |
US6469311B1 (en) * | 1997-07-16 | 2002-10-22 | Molecular Devices Corporation | Detection device for light transmitted from a sensed volume |
US6248988B1 (en) * | 1998-05-05 | 2001-06-19 | Kla-Tencor Corporation | Conventional and confocal multi-spot scanning optical microscope |
US7295314B1 (en) * | 1998-07-10 | 2007-11-13 | Nanometrics Incorporated | Metrology/inspection positioning system |
US6208411B1 (en) | 1998-09-28 | 2001-03-27 | Kla-Tencor Corporation | Massively parallel inspection and imaging system |
JP2001083391A (ja) * | 1999-09-13 | 2001-03-30 | Nikon Corp | 顕微鏡のフォーカス装置 |
US6867851B2 (en) * | 1999-11-04 | 2005-03-15 | Regents Of The University Of Minnesota | Scanning of biological samples |
DE19957413A1 (de) * | 1999-11-29 | 2001-05-31 | Leica Microsystems | Vorrichtung zum optischen Abtasten mehrerer Objekte |
US6711283B1 (en) * | 2000-05-03 | 2004-03-23 | Aperio Technologies, Inc. | Fully automatic rapid microscope slide scanner |
JP2002071566A (ja) * | 2000-08-24 | 2002-03-08 | Hitachi Ltd | 蛍光、燐光測定方法 |
US6818907B2 (en) * | 2000-10-17 | 2004-11-16 | The President And Fellows Of Harvard College | Surface plasmon enhanced illumination system |
US6538444B2 (en) * | 2001-07-27 | 2003-03-25 | The University Of Chicago | Two dimensional B1-gradient NMR imager |
CN1991335A (zh) * | 2002-09-30 | 2007-07-04 | 独立行政法人科学技术振兴机构 | 共焦点显微镜及采用它的荧光测量方法和偏振光测量方法 |
US6943881B2 (en) * | 2003-06-04 | 2005-09-13 | Tomophase Corporation | Measurements of optical inhomogeneity and other properties in substances using propagation modes of light |
FI20030867A (fi) * | 2003-06-10 | 2004-12-11 | Wallac Oy | Optinen mittausmenetelmä ja laboratoriomittauslaite |
US20060226374A1 (en) * | 2003-08-06 | 2006-10-12 | Gnothis Holding S.A. | Method and device for identifying luminescent molecules according to the fluorescence correlation spectroscopy method |
US7323681B1 (en) * | 2003-09-11 | 2008-01-29 | Applera Corporation | Image enhancement by sub-pixel imaging |
US7295316B2 (en) * | 2004-01-14 | 2007-11-13 | Applera Corporation | Fluorescent detector with automatic changing filters |
US7248360B2 (en) * | 2004-04-02 | 2007-07-24 | Ppd Biomarker Discovery Sciences, Llc | Polychronic laser scanning system and method of use |
CN100583434C (zh) * | 2004-06-07 | 2010-01-20 | 先锋生物科技股份有限公司 | 用于微流体器件的光学透镜系统和方法 |
US7623289B2 (en) * | 2004-07-22 | 2009-11-24 | Olympus Corporation | Observation apparatus having thermoregulation mechanism |
AU2006210424A1 (en) | 2005-01-31 | 2006-08-10 | The Board Of Trustees Of The University Of Illinois | Methods and devices for characterizing particles in clear and turbid media |
US7489393B2 (en) | 2005-03-02 | 2009-02-10 | Kla-Tencor Technologies Corporation | Enhanced simultaneous multi-spot inspection and imaging |
EP1915607A1 (en) * | 2005-07-19 | 2008-04-30 | Physical Sciences, Inc. | Side view imaging microwell array |
DE102005039087B3 (de) * | 2005-08-04 | 2007-03-29 | Bruker Biospin Gmbh | Probenkopf für Kernresonanzmessungen |
US20070161876A1 (en) * | 2005-11-18 | 2007-07-12 | Spectrx, Inc. | Method and apparatus for rapid detection and diagnosis of tissue abnormalities |
WO2007091530A1 (ja) * | 2006-02-07 | 2007-08-16 | The Furukawa Electric Co., Ltd. | 光検出装置及び測定対象読取装置 |
US7692783B2 (en) | 2006-02-13 | 2010-04-06 | Pacific Biosciences Of California | Methods and systems for simultaneous real-time monitoring of optical signals from multiple sources |
JP2008116395A (ja) * | 2006-11-07 | 2008-05-22 | Fujitsu Ltd | 蛍光検出装置 |
JP2008241686A (ja) * | 2007-02-26 | 2008-10-09 | Fujitsu Ltd | 標的検出装置及び標的検出方法 |
WO2009009178A2 (en) * | 2007-04-06 | 2009-01-15 | The General Hospital Corporation | Systems and methods for optical imaging using early arriving photons |
AU2009273838A1 (en) * | 2008-07-25 | 2010-01-28 | Oregon Health & Science University | Rapid confocal microscopy to support surgical procedures |
US7982875B2 (en) * | 2009-06-15 | 2011-07-19 | Wyatt Technology Corporation | Method and apparatus for measuring the scattered light signals from a liquid sample |
WO2011069261A1 (en) * | 2009-12-08 | 2011-06-16 | Spectral Applied Research Inc. | Imaging distal end of multimode fiber |
US9068916B2 (en) * | 2010-03-15 | 2015-06-30 | Bio-Rad Laboratories, Inc. | Microassembled imaging flow cytometer |
US8699025B2 (en) * | 2010-04-01 | 2014-04-15 | Stephen H. Hall | Method and apparatus for measuring hexavalent chromium in water |
CN103026205B (zh) * | 2010-07-26 | 2016-03-23 | 奥林巴斯株式会社 | 使用发光探针检测溶液中稀疏颗粒的方法 |
WO2012037182A1 (en) * | 2010-09-14 | 2012-03-22 | Applied Precision, Inc. | Oblique-illumination systems and methods |
US9494783B2 (en) * | 2010-11-30 | 2016-11-15 | Etaluma Inc. | Compact, high-resolution fluorescence and brightfield microscope and methods of use |
EP2535755A1 (en) * | 2011-06-14 | 2012-12-19 | Ecole Polytechnique Fédérale de Lausanne (EPFL) | Cumulant microscopy |
DE102011105181A1 (de) * | 2011-06-17 | 2012-12-20 | Leica Microsystems Cms Gmbh | Mikroskop und Verfahren zur bildgebenden Fluoreszenzmikroskopie |
DE102011083215A1 (de) * | 2011-09-22 | 2013-03-28 | Siemens Aktiengesellschaft | Probenträger und Verfahren zur mikroskopischen Untersuchung biologischer Proben |
CN202256191U (zh) * | 2011-09-22 | 2012-05-30 | 中海阳新能源电力股份有限公司 | 镀膜玻璃内部缺陷断层显示装置 |
WO2013109957A1 (en) * | 2012-01-18 | 2013-07-25 | University Of Utah Research Foundation | Devices and systems for fluorescence imaging of tissue |
JP5918009B2 (ja) | 2012-05-11 | 2016-05-18 | 株式会社日立ハイテクノロジーズ | 欠陥検査方法および欠陥検査装置 |
DE102012211943A1 (de) * | 2012-07-09 | 2014-06-12 | Carl Zeiss Microscopy Gmbh | Mikroskop |
US10101274B2 (en) * | 2012-12-05 | 2018-10-16 | Genepoc Inc. | Optical interrogation device |
AU2013369397B2 (en) * | 2012-12-26 | 2016-05-26 | Ventana Medical Systems, Inc. | Opposables and automated specimen processing systems with opposables |
US9594019B1 (en) * | 2013-08-09 | 2017-03-14 | Lester F. Ludwig | Optical tomography for microscopy, cell cytometry, microplate array instrumentation, crystallography, and other applications |
US9791385B2 (en) * | 2013-12-10 | 2017-10-17 | Arizona Board Of Regents On Behalf Of Arizona State University | Modular high resolution X-ray computed tomography system |
WO2015164844A1 (en) * | 2014-04-24 | 2015-10-29 | Vutara, Inc. | Super resolution microscopy |
WO2016030464A1 (en) | 2014-08-28 | 2016-03-03 | Single Technologies Ab | High throughput biochemical screening |
US9891177B2 (en) * | 2014-10-03 | 2018-02-13 | Kla-Tencor Corporation | TDI sensor in a darkfield system |
US10928619B2 (en) * | 2014-10-06 | 2021-02-23 | Leica Microsystems (Schweiz) Ag | Microscope |
JP6375239B2 (ja) * | 2015-02-05 | 2018-08-15 | オリンパス株式会社 | レーザ顕微鏡装置 |
US9677988B1 (en) * | 2015-07-10 | 2017-06-13 | David E. Doggett | Integrating radiation collection and detection apparatus |
US9366617B1 (en) * | 2015-07-10 | 2016-06-14 | David E. Doggett | Self-stirring container |
US10802262B2 (en) * | 2015-10-29 | 2020-10-13 | The Board Of Trustees Of The Leland Stanford Junior University | Methods and systems for imaging a biological sample |
-
2017
- 2017-02-23 CN CN201780013225.2A patent/CN108700520B/zh active Active
- 2017-02-23 WO PCT/EP2017/054244 patent/WO2017144619A1/en active Application Filing
- 2017-02-23 EP EP17707260.0A patent/EP3420338B1/en active Active
- 2017-02-23 JP JP2018544238A patent/JP6951354B2/ja active Active
- 2017-02-23 US US16/079,900 patent/US10732113B2/en active Active
Also Published As
Publication number | Publication date |
---|---|
WO2017144619A1 (en) | 2017-08-31 |
US10732113B2 (en) | 2020-08-04 |
EP3420338B1 (en) | 2023-05-03 |
JP2019508691A (ja) | 2019-03-28 |
EP3420338A1 (en) | 2019-01-02 |
CN108700520A (zh) | 2018-10-23 |
CN108700520B (zh) | 2021-12-07 |
US20190049382A1 (en) | 2019-02-14 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
JP7055793B2 (ja) | 選択的平面照明を伴う明視野顕微鏡 | |
CN101228428B (zh) | 荧光纳米显微方法 | |
Ortega-Arroyo et al. | Interferometric scattering microscopy (iSCAT): new frontiers in ultrafast and ultrasensitive optical microscopy | |
JP7099823B2 (ja) | ハイスループット生化学的スクリーニング | |
JP6951354B2 (ja) | 高スループットの撮像のための方法及びデバイス | |
JP5250152B2 (ja) | 光分析装置、光分析方法並びに光分析用コンピュータプログラム | |
US8681332B2 (en) | Method of measuring a diffusion characteristic value of a particle | |
US7738945B2 (en) | Method and apparatus for pseudo-projection formation for optical tomography | |
US10942120B2 (en) | High throughput method and apparatus for measuring multiple optical properties of a liquid sample | |
WO2008057159A2 (en) | Apparatus and method for tracking a molecule or particle in three dimensions | |
US20200183140A1 (en) | Dual parallel optical axis modules sharing sample stage for bioburden testing | |
JP2004361087A (ja) | 生体分子解析装置 | |
Sheppard | Confocal microscopy–principles, practice and options | |
US20200132976A1 (en) | Luminescence imaging apparatus and methods | |
JP2004361086A (ja) | 生体分子解析装置 | |
JP2012154885A (ja) | 単一発光粒子の光を検出し分析するための光分析装置及び光分析方法 |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
A521 | Request for written amendment filed |
Free format text: JAPANESE INTERMEDIATE CODE: A523 Effective date: 20181023 |
|
A621 | Written request for application examination |
Free format text: JAPANESE INTERMEDIATE CODE: A621 Effective date: 20200212 |
|
A977 | Report on retrieval |
Free format text: JAPANESE INTERMEDIATE CODE: A971007 Effective date: 20201021 |
|
A131 | Notification of reasons for refusal |
Free format text: JAPANESE INTERMEDIATE CODE: A131 Effective date: 20201110 |
|
A521 | Request for written amendment filed |
Free format text: JAPANESE INTERMEDIATE CODE: A523 Effective date: 20210210 |
|
TRDD | Decision of grant or rejection written | ||
A01 | Written decision to grant a patent or to grant a registration (utility model) |
Free format text: JAPANESE INTERMEDIATE CODE: A01 Effective date: 20210824 |
|
A61 | First payment of annual fees (during grant procedure) |
Free format text: JAPANESE INTERMEDIATE CODE: A61 Effective date: 20210924 |
|
R150 | Certificate of patent or registration of utility model |
Ref document number: 6951354 Country of ref document: JP Free format text: JAPANESE INTERMEDIATE CODE: R150 |