JP6947647B2 - 遺伝子改変t細胞の選択方法 - Google Patents

遺伝子改変t細胞の選択方法 Download PDF

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JP6947647B2
JP6947647B2 JP2017562971A JP2017562971A JP6947647B2 JP 6947647 B2 JP6947647 B2 JP 6947647B2 JP 2017562971 A JP2017562971 A JP 2017562971A JP 2017562971 A JP2017562971 A JP 2017562971A JP 6947647 B2 JP6947647 B2 JP 6947647B2
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cells
tyms
dhfr
transgene
cell
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JP2018506995A5 (enExample
JP2018506995A (ja
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デイビッド ラッシュワース,
デイビッド ラッシュワース,
ローレンス ジェイ. クーパー,
ローレンス ジェイ. クーパー,
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The University of Texas System
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JP2017562971A 2015-02-24 2016-02-24 遺伝子改変t細胞の選択方法 Expired - Fee Related JP6947647B2 (ja)

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Application Number Priority Date Filing Date Title
US201562120329P 2015-02-24 2015-02-24
US62/120,329 2015-02-24
US201562120790P 2015-02-25 2015-02-25
US62/120,790 2015-02-25
US201562175794P 2015-06-15 2015-06-15
US62/175,794 2015-06-15
PCT/US2016/019288 WO2016138091A2 (en) 2015-02-24 2016-02-24 Selection methods for genetically-modified t cells

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JP2018506995A JP2018506995A (ja) 2018-03-15
JP2018506995A5 JP2018506995A5 (enExample) 2019-03-14
JP6947647B2 true JP6947647B2 (ja) 2021-10-13

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Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2997134B1 (en) 2013-05-14 2020-06-24 Board of Regents, The University of Texas System Human application of engineered chimeric antigen receptor (car) t-cells
DK3105335T3 (da) 2014-02-14 2020-01-13 Univ Texas Kimære antigenreceptorer og fremgangsmåder til fremstilling heraf
CA2964783C (en) 2014-11-05 2024-01-23 Board Of Regents, The University Of Texas System Chimeric antigen receptors (car) to selectively target protein complexes
JP6775515B2 (ja) 2015-03-11 2020-10-28 ボード・オブ・リージエンツ,ザ・ユニバーシテイ・オブ・テキサス・システム トランスポザーゼポリペプチド及びその使用
EP3349791A1 (en) 2015-09-15 2018-07-25 Board of Regents, The University of Texas System T-cell receptor (tcr)-binding antibodies and uses thereof
WO2017075147A1 (en) 2015-10-27 2017-05-04 Board Of Regents, The University Of Texas System Chimeric antigen receptor molecules and uses thereof
US11629340B2 (en) 2017-03-03 2023-04-18 Obsidian Therapeutics, Inc. DHFR tunable protein regulation
CA3064000A1 (en) 2017-05-24 2018-11-29 Effector Therapeutics, Inc. Methods and compositions for cellular immunotherapy
CN109136282A (zh) * 2018-09-30 2019-01-04 北京鼎成肽源生物技术有限公司 一种hafft1细胞的构建方法
CA3119599A1 (en) 2018-11-16 2020-05-22 Rapa Therapeutics, Llc Als treatment using induced regulatory t (itreg) cells
WO2020102726A1 (en) * 2018-11-16 2020-05-22 Rapa Therapeutics, Llc Method for manufacturing of human hybrid regulatory t/th2 cells (hybrid treg/th2 cells) from de-differentiated t cells
KR102594009B1 (ko) 2021-02-01 2023-10-24 서울대학교산학협력단 형질전환 생식세포의 제조 방법 및 이를 이용한 형질전환 동물의 제조 방법
EP4408169A4 (en) * 2021-09-30 2025-10-29 Univ Southern California Human hepatocyte culture medium and conditioned medium of in vitro cultured human hepatocyte cells and their uses
CN114740196B (zh) * 2022-04-08 2023-04-18 中南大学湘雅二医院 标志物及其在制备评价机体免疫功能的产品中的应用

Family Cites Families (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
MX340972B (es) * 2008-10-08 2016-08-02 Intrexon Corp Celulas manipuladas por ingenieria que expresan multiples inmunomoduladores, y uso de las mismas.
DK3246042T3 (da) * 2009-11-02 2019-10-14 Univ Emory Lægemiddelresistent immunterapi til behandling af cancer
PH12013501201A1 (en) 2010-12-09 2013-07-29 Univ Pennsylvania Use of chimeric antigen receptor-modified t cells to treat cancer
AU2012203048A1 (en) * 2011-05-24 2012-12-13 Agency For Science, Technology And Research IRES mediated multicistronic vectors
WO2013074916A1 (en) 2011-11-18 2013-05-23 Board Of Regents, The University Of Texas System Car+ t cells genetically modified to eliminate expression of t- cell receptor and/or hla
EP2814846B1 (en) * 2012-02-13 2020-01-08 Seattle Children's Hospital d/b/a Seattle Children's Research Institute Bispecific chimeric antigen receptors and therapeutic uses thereof
EP2997134B1 (en) 2013-05-14 2020-06-24 Board of Regents, The University of Texas System Human application of engineered chimeric antigen receptor (car) t-cells
CA2913052A1 (en) 2013-05-24 2014-11-27 Board Of Regents, The University Of Texas System Chimeric antigen receptor-targeting monoclonal antibodies
US9907820B2 (en) 2013-10-25 2018-03-06 Board Of Regents, The University Of Texas System Polyclonal gamma delta T cells for immunotherapy
JP6976058B2 (ja) * 2013-11-22 2021-12-01 セレクティスCellectis 免疫療法のための化学療法薬耐性t細胞を工学的に作製する方法
DK3105335T3 (da) 2014-02-14 2020-01-13 Univ Texas Kimære antigenreceptorer og fremgangsmåder til fremstilling heraf
SG10201808811QA (en) * 2014-04-10 2018-11-29 Seattle Childrens Hospital Dba Seattle Childrens Res Inst Method and compositions for cellular immunotherapy
US20170158749A1 (en) 2014-04-23 2017-06-08 Board Of Regents, The University Of Texas System Chimeric antigen receptors (car) and methods for making and using the same
KR20160145186A (ko) 2014-04-24 2016-12-19 보드 오브 리전츠, 더 유니버시티 오브 텍사스 시스템 입양 세포 요법 생성물을 생성하기 위한 유도 만능 줄기 세포의 응용
CA2964785A1 (en) 2014-11-05 2016-05-12 Board Of Regents, The University Of Texas System Gene modified immune effector cells and engineered cells for expansion of immune effector cells
CA2964783C (en) 2014-11-05 2024-01-23 Board Of Regents, The University Of Texas System Chimeric antigen receptors (car) to selectively target protein complexes
JP6775515B2 (ja) 2015-03-11 2020-10-28 ボード・オブ・リージエンツ,ザ・ユニバーシテイ・オブ・テキサス・システム トランスポザーゼポリペプチド及びその使用
EP3349791A1 (en) 2015-09-15 2018-07-25 Board of Regents, The University of Texas System T-cell receptor (tcr)-binding antibodies and uses thereof
WO2017075147A1 (en) 2015-10-27 2017-05-04 Board Of Regents, The University Of Texas System Chimeric antigen receptor molecules and uses thereof

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HK1248555A1 (zh) 2018-10-19
WO2016138091A3 (en) 2016-12-29
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KR20170117095A (ko) 2017-10-20
US10808230B2 (en) 2020-10-20
US20180298349A1 (en) 2018-10-18
EP3804741A3 (en) 2021-07-14
CA2976126A1 (en) 2016-09-01
AU2021203606A1 (en) 2021-07-01
CN107667169A (zh) 2018-02-06
CN107667169B (zh) 2021-10-29
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WO2016138091A2 (en) 2016-09-01

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