JP6941568B2 - ヌクレアーゼを使用する野生型dnaの選択的分解および突然変異体対立遺伝子の濃縮 - Google Patents
ヌクレアーゼを使用する野生型dnaの選択的分解および突然変異体対立遺伝子の濃縮 Download PDFInfo
- Publication number
- JP6941568B2 JP6941568B2 JP2017566374A JP2017566374A JP6941568B2 JP 6941568 B2 JP6941568 B2 JP 6941568B2 JP 2017566374 A JP2017566374 A JP 2017566374A JP 2017566374 A JP2017566374 A JP 2017566374A JP 6941568 B2 JP6941568 B2 JP 6941568B2
- Authority
- JP
- Japan
- Prior art keywords
- nucleic acid
- wild
- target
- probe
- type
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/26—Preparation of nitrogen-containing carbohydrates
- C12P19/28—N-glycosides
- C12P19/30—Nucleotides
- C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1093—General methods of preparing gene libraries, not provided for in other subgroups
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
- C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Biochemistry (AREA)
- Analytical Chemistry (AREA)
- Biomedical Technology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Immunology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Crystallography & Structural Chemistry (AREA)
- Plant Pathology (AREA)
- Bioinformatics & Computational Biology (AREA)
- General Chemical & Material Sciences (AREA)
- Pathology (AREA)
- Medicinal Chemistry (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Enzymes And Modification Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201562183854P | 2015-06-24 | 2015-06-24 | |
| US62/183,854 | 2015-06-24 | ||
| PCT/US2016/039167 WO2016210224A1 (en) | 2015-06-24 | 2016-06-24 | Selective degradation of wild-type dna and enrichment of mutant alleles using nuclease |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2018518967A JP2018518967A (ja) | 2018-07-19 |
| JP2018518967A5 JP2018518967A5 (enExample) | 2019-07-25 |
| JP6941568B2 true JP6941568B2 (ja) | 2021-09-29 |
Family
ID=57585693
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2017566374A Active JP6941568B2 (ja) | 2015-06-24 | 2016-06-24 | ヌクレアーゼを使用する野生型dnaの選択的分解および突然変異体対立遺伝子の濃縮 |
Country Status (7)
| Country | Link |
|---|---|
| US (2) | US11725230B2 (enExample) |
| EP (1) | EP3313992B1 (enExample) |
| JP (1) | JP6941568B2 (enExample) |
| AU (1) | AU2016281718B2 (enExample) |
| CA (1) | CA2990846C (enExample) |
| ES (1) | ES2917175T3 (enExample) |
| WO (1) | WO2016210224A1 (enExample) |
Families Citing this family (22)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA2990846C (en) | 2015-06-24 | 2023-09-26 | Dana-Farber Cancer Institute, Inc. | Selective degradation of wild-type dna and enrichment of mutant alleles using nuclease |
| US11371090B2 (en) | 2016-12-12 | 2022-06-28 | Dana-Farber Cancer Institute, Inc. | Compositions and methods for molecular barcoding of DNA molecules prior to mutation enrichment and/or mutation detection |
| US11174511B2 (en) | 2017-07-24 | 2021-11-16 | Dana-Farber Cancer Institute, Inc. | Methods and compositions for selecting and amplifying DNA targets in a single reaction mixture |
| CN107893109B (zh) * | 2017-11-08 | 2021-07-06 | 重庆邮电大学 | 一种基于移除野生型序列的低丰度基因突变富集方法 |
| KR102086689B1 (ko) * | 2018-02-06 | 2020-03-09 | 주식회사 진씨커 | 돌연변이 세포 유리 유전자 분리 키트 및 이를 이용한 돌연변이 세포 유리 유전자 분리 방법 |
| WO2019195268A2 (en) | 2018-04-02 | 2019-10-10 | Grail, Inc. | Methylation markers and targeted methylation probe panels |
| CN108531603A (zh) * | 2018-05-31 | 2018-09-14 | 青岛普泽麦迪生物技术有限公司 | 一种用于braf基因v600e突变检测的引物对、试剂盒及其检测方法 |
| AU2019351130B2 (en) | 2018-09-27 | 2025-10-23 | GRAIL, Inc | Methylation markers and targeted methylation probe panel |
| CN113260451A (zh) * | 2018-10-16 | 2021-08-13 | 辛辛那提大学 | 单分子表观遗传定位 |
| CN109880891B (zh) * | 2019-04-22 | 2021-07-30 | 上海交通大学 | 基于核酸酶偶联pcr原理富集低丰度dna突变的检测技术体系及应用 |
| CA3145319A1 (en) * | 2019-08-21 | 2021-02-25 | Dana-Farber Cancer Institute, Inc. | Multi-site enrichment of deletions in dna microsatellites |
| WO2022032194A1 (en) | 2020-08-06 | 2022-02-10 | Singular Genomics Systems, Inc. | Methods for in situ transcriptomics and proteomics |
| WO2022032195A2 (en) | 2020-08-06 | 2022-02-10 | Singular Genomics Systems, Inc. | Spatial sequencing |
| GB2603203A (en) * | 2021-02-02 | 2022-08-03 | Univ Heriot Watt | Method for enriching nucelic acids |
| EP4337782A4 (en) * | 2021-05-14 | 2025-04-02 | Carmel-Haifa University Economic Corporation Ltd. | A method of identifying ultra-rare genetic variants |
| US11993792B2 (en) | 2021-05-27 | 2024-05-28 | New England Biolabs, Inc. | DNase I variants, compositions, methods, and kits |
| GB202111195D0 (en) * | 2021-08-03 | 2021-09-15 | Cergentis B V | Method for targeted sequencing |
| CN114280128B (zh) * | 2021-12-24 | 2022-11-18 | 清华大学 | 双标记gFET的制备及其在miRNA检测中的应用 |
| CN116240200B (zh) * | 2022-07-01 | 2025-03-11 | 中国科学院基础医学与肿瘤研究所(筹) | 一种基于可编程核酸酶的超灵敏目标核酸富集检测方法 |
| EP4605546A1 (en) * | 2022-10-19 | 2025-08-27 | Biofidelity Ltd | Nucleic acid enrichment and detection |
| WO2024249961A1 (en) | 2023-06-01 | 2024-12-05 | Singular Genomics Systems, Inc. | Methods and probes for detecting polynucleotide sequences in cells and tissues |
| WO2025029475A1 (en) * | 2023-07-28 | 2025-02-06 | Guardant Health, Inc. | Methods to enrich nucleotide variants by negative selection |
Family Cites Families (31)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AU661505B2 (en) | 1990-05-07 | 1995-07-27 | Tapestry Pharmaceuticals, Inc. | Diagnostic applications of double D-loop formation |
| EP0672185A4 (en) * | 1992-07-17 | 1997-04-23 | Aprogenex Inc | NUCLEIC ACID SAMPLE AND THEIR USE IN DETECTING DOUBLE-STRANDED NUCLEIC ACIDS. |
| US5629178A (en) | 1994-10-28 | 1997-05-13 | Genetics & Ivf Institute | Method for enhancing amplification in the polymerase chain reaction employing peptide nucleic acid (PNA) |
| US6949340B2 (en) * | 2001-03-28 | 2005-09-27 | Creative Mines Llc | Optical phase modulator |
| DE10158516A1 (de) | 2001-11-29 | 2003-06-12 | Focusgenomics Gmbh | Verfahren zum Nachweis von Hybridisierungsereignissen in Nukleinsäuren |
| AU2002346662A1 (en) * | 2001-12-04 | 2003-06-17 | Euregen Llc | Methods and compositions for selectively cleaving dna containing duplex acids in a complex nucleic acid mixture |
| EP1725682B1 (en) | 2004-03-08 | 2015-09-09 | Rubicon Genomics, Inc. | Methods and compositions for generating and amplifying dna libraries for sensitive detection and analysis of dna methylation |
| US7749707B2 (en) | 2005-03-01 | 2010-07-06 | Wako Pure Chemical Industries, Ltd. | Method for obtaining subtraction polynucleotide |
| AU2006316537A1 (en) * | 2005-11-24 | 2007-05-31 | Basf Se | Chimeric keratin-binding effector proteins |
| US7544473B2 (en) | 2006-01-23 | 2009-06-09 | Population Genetics Technologies Ltd. | Nucleic acid analysis using sequence tokens |
| WO2008096146A1 (en) | 2007-02-07 | 2008-08-14 | Solexa Limited | Preparation of templates for methylation analysis |
| US20080254453A1 (en) | 2007-04-12 | 2008-10-16 | Affymetrix, Inc | Analysis of methylation using selective adaptor ligation |
| US7749708B2 (en) | 2007-09-12 | 2010-07-06 | Transgenomic, Inc. | Method for identifying the sequence of one or more variant nucleotides in a nucleic acid molecule |
| US20100216648A1 (en) * | 2009-02-20 | 2010-08-26 | Febit Holding Gmbh | Synthesis of sequence-verified nucleic acids |
| US10636413B2 (en) | 2009-06-13 | 2020-04-28 | Rolr, Inc. | System for communication skills training using juxtaposition of recorded takes |
| EP2272976A1 (en) * | 2009-07-06 | 2011-01-12 | Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V. | Method for differentiation of polynucleotide strands |
| WO2011057354A1 (en) | 2009-11-13 | 2011-05-19 | Commonwealth Scientific And Industrial Research Organisation | Epigenetic analysis |
| JP5795341B2 (ja) | 2010-03-08 | 2015-10-14 | デイナ ファーバー キャンサー インスティチュート,インコーポレイテッド | 参照ブロック配列によるfullCOLD−PCR濃縮 |
| US9260753B2 (en) | 2011-03-24 | 2016-02-16 | President And Fellows Of Harvard College | Single cell nucleic acid detection and analysis |
| WO2012135053A2 (en) * | 2011-03-25 | 2012-10-04 | Integrated Dna Technologies, Inc. | Rnase h-based assays utilizing modified rna monomers |
| CA2830361C (en) | 2011-03-31 | 2021-03-16 | Dana-Farber Cancer Institute, Inc. | Methods and compositions to enable multiplex cold-pcr |
| CN103717751A (zh) | 2011-05-19 | 2014-04-09 | 塞昆纳姆股份有限公司 | 用于多重核酸鉴定的产品和方法 |
| US9238836B2 (en) * | 2012-03-30 | 2016-01-19 | Pacific Biosciences Of California, Inc. | Methods and compositions for sequencing modified nucleic acids |
| KR102126744B1 (ko) * | 2013-03-14 | 2020-06-26 | 다카라 바이오 가부시키가이샤 | 내열성 미스매치 엔도뉴클레아제의 이용방법 |
| CN111383715B (zh) | 2013-04-17 | 2024-09-10 | 先锋国际良种公司 | 用于在基因组中表征dna序列组成的方法 |
| US10913977B2 (en) | 2013-07-24 | 2021-02-09 | Dana-Farber Cancer Institute, Inc. | Methods and compositions to enable enrichment of minor DNA alleles by limiting denaturation time in PCR or simply enable enrichment of minor DNA alleles by limiting the denaturation time in PCR |
| US9873908B2 (en) | 2013-11-27 | 2018-01-23 | Roche Molecular Systems, Inc. | Methods for the enrichment of mutated nucleic acid from a mixture |
| EP3378952B1 (en) | 2013-12-28 | 2020-02-05 | Guardant Health, Inc. | Methods and systems for detecting genetic variants |
| CA2990846C (en) | 2015-06-24 | 2023-09-26 | Dana-Farber Cancer Institute, Inc. | Selective degradation of wild-type dna and enrichment of mutant alleles using nuclease |
| US11371090B2 (en) | 2016-12-12 | 2022-06-28 | Dana-Farber Cancer Institute, Inc. | Compositions and methods for molecular barcoding of DNA molecules prior to mutation enrichment and/or mutation detection |
| CA3145319A1 (en) | 2019-08-21 | 2021-02-25 | Dana-Farber Cancer Institute, Inc. | Multi-site enrichment of deletions in dna microsatellites |
-
2016
- 2016-06-24 CA CA2990846A patent/CA2990846C/en active Active
- 2016-06-24 JP JP2017566374A patent/JP6941568B2/ja active Active
- 2016-06-24 EP EP16815352.6A patent/EP3313992B1/en active Active
- 2016-06-24 WO PCT/US2016/039167 patent/WO2016210224A1/en not_active Ceased
- 2016-06-24 AU AU2016281718A patent/AU2016281718B2/en active Active
- 2016-06-24 US US15/739,301 patent/US11725230B2/en active Active
- 2016-06-24 ES ES16815352T patent/ES2917175T3/es active Active
-
2023
- 2023-08-13 US US18/448,941 patent/US20230392191A1/en active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| HK1254317A1 (zh) | 2019-07-19 |
| WO2016210224A1 (en) | 2016-12-29 |
| CA2990846A1 (en) | 2016-12-29 |
| ES2917175T3 (es) | 2022-07-07 |
| JP2018518967A (ja) | 2018-07-19 |
| US11725230B2 (en) | 2023-08-15 |
| US20180187242A1 (en) | 2018-07-05 |
| EP3313992A1 (en) | 2018-05-02 |
| EP3313992B1 (en) | 2022-03-23 |
| AU2016281718A1 (en) | 2017-12-14 |
| CA2990846C (en) | 2023-09-26 |
| US20230392191A1 (en) | 2023-12-07 |
| AU2016281718B2 (en) | 2022-03-31 |
| EP3313992A4 (en) | 2018-11-14 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US20230392191A1 (en) | Selective degradation of wild-type dna and enrichment of mutant alleles using nuclease | |
| JP7112456B2 (ja) | バリアント検出のための方法 | |
| JP5237126B2 (ja) | ライゲーションアッセイを用いてハイスループットシークエンスに基づき遺伝子関連配列を検出する方法 | |
| CN107849603B (zh) | 利用有限核苷酸组成的引物扩增 | |
| Arbeithuber et al. | Artifactual mutations resulting from DNA lesions limit detection levels in ultrasensitive sequencing applications | |
| EP1255871B1 (en) | Multiplex ligatable probe amplification | |
| JP4663118B2 (ja) | ヌクレオチドの変異について核酸をスクリーニングする方法 | |
| JP2019004914A (ja) | ブロッキングオリゴヌクレオチドを用いたdna増幅の方法 | |
| KR20120139780A (ko) | 기준 차단 서열을 이용한 완전 cold-pcr 풍부화 | |
| JP2018506307A (ja) | 非特異的増幅産物を減少させる為の方法及び組成物 | |
| US10023908B2 (en) | Nucleic acid amplification method using allele-specific reactive primer | |
| EP4017998B1 (en) | Multi-site enrichment of deletions in dna microsatellites | |
| EP4267763B1 (en) | Compositions and methods for highly sensitive detection of target sequences in multiplex reactions | |
| WO2001044504A2 (en) | Method for detecting methylated cpg-containing nucleic acid | |
| JP5914346B2 (ja) | 非メチル化核酸の選択的蓄積 | |
| HK1254317B (en) | Selective degradation of wild-type dna and enrichment of mutant alleles using nuclease | |
| HK40064470A (en) | Amplification with primers of limited nucleotide composition |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| A521 | Request for written amendment filed |
Free format text: JAPANESE INTERMEDIATE CODE: A523 Effective date: 20190619 |
|
| A621 | Written request for application examination |
Free format text: JAPANESE INTERMEDIATE CODE: A621 Effective date: 20190619 |
|
| A131 | Notification of reasons for refusal |
Free format text: JAPANESE INTERMEDIATE CODE: A131 Effective date: 20200807 |
|
| A521 | Request for written amendment filed |
Free format text: JAPANESE INTERMEDIATE CODE: A523 Effective date: 20201106 |
|
| A131 | Notification of reasons for refusal |
Free format text: JAPANESE INTERMEDIATE CODE: A131 Effective date: 20210304 |
|
| A521 | Request for written amendment filed |
Free format text: JAPANESE INTERMEDIATE CODE: A523 Effective date: 20210602 |
|
| TRDD | Decision of grant or rejection written | ||
| A01 | Written decision to grant a patent or to grant a registration (utility model) |
Free format text: JAPANESE INTERMEDIATE CODE: A01 Effective date: 20210816 |
|
| A61 | First payment of annual fees (during grant procedure) |
Free format text: JAPANESE INTERMEDIATE CODE: A61 Effective date: 20210906 |
|
| R150 | Certificate of patent or registration of utility model |
Ref document number: 6941568 Country of ref document: JP Free format text: JAPANESE INTERMEDIATE CODE: R150 |
|
| RD02 | Notification of acceptance of power of attorney |
Free format text: JAPANESE INTERMEDIATE CODE: R3D02 |
|
| RD04 | Notification of resignation of power of attorney |
Free format text: JAPANESE INTERMEDIATE CODE: R3D04 |
|
| R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
| R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |