HK1254317A1 - 使用核酸酶的野生型dna的選擇性降解以及突變體等位基因的富集 - Google Patents
使用核酸酶的野生型dna的選擇性降解以及突變體等位基因的富集Info
- Publication number
- HK1254317A1 HK1254317A1 HK18113285.7A HK18113285A HK1254317A1 HK 1254317 A1 HK1254317 A1 HK 1254317A1 HK 18113285 A HK18113285 A HK 18113285A HK 1254317 A1 HK1254317 A1 HK 1254317A1
- Authority
- HK
- Hong Kong
- Prior art keywords
- nuclease
- enrichment
- wild
- type dna
- mutant alleles
- Prior art date
Links
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/26—Preparation of nitrogen-containing carbohydrates
- C12P19/28—N-glycosides
- C12P19/30—Nucleotides
- C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1093—General methods of preparing gene libraries, not provided for in other subgroups
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
- C12N9/22—Ribonucleases RNAses, DNAses
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Biophysics (AREA)
- Biomedical Technology (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Immunology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Crystallography & Structural Chemistry (AREA)
- Plant Pathology (AREA)
- Bioinformatics & Computational Biology (AREA)
- Medicinal Chemistry (AREA)
- General Chemical & Material Sciences (AREA)
- Pathology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Enzymes And Modification Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US201562183854P | 2015-06-24 | 2015-06-24 | |
PCT/US2016/039167 WO2016210224A1 (en) | 2015-06-24 | 2016-06-24 | Selective degradation of wild-type dna and enrichment of mutant alleles using nuclease |
Publications (1)
Publication Number | Publication Date |
---|---|
HK1254317A1 true HK1254317A1 (zh) | 2019-07-19 |
Family
ID=57585693
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
HK18113285.7A HK1254317A1 (zh) | 2015-06-24 | 2018-10-17 | 使用核酸酶的野生型dna的選擇性降解以及突變體等位基因的富集 |
Country Status (8)
Country | Link |
---|---|
US (2) | US11725230B2 (zh) |
EP (1) | EP3313992B1 (zh) |
JP (1) | JP6941568B2 (zh) |
AU (1) | AU2016281718B2 (zh) |
CA (1) | CA2990846C (zh) |
ES (1) | ES2917175T3 (zh) |
HK (1) | HK1254317A1 (zh) |
WO (1) | WO2016210224A1 (zh) |
Families Citing this family (17)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
ES2917175T3 (es) | 2015-06-24 | 2022-07-07 | Dana Farber Cancer Inst Inc | Degradación selectiva de ADN de tipo salvaje y enriquecimiento de alelos mutantes usando nucleasa |
CA3046953A1 (en) | 2016-12-12 | 2018-06-21 | Dana Farber Cancer Institute, Inc. | Compositions and methods for molecular barcoding of dna molecules prior to mutation enrichment and/or mutation detection |
WO2019023243A1 (en) | 2017-07-24 | 2019-01-31 | Dana-Farber Cancer Institute, Inc. | METHODS AND COMPOSITIONS FOR SELECTING AND AMPLIFYING DNA TARGETS IN A SINGLE REACTION MIXTURE |
CN107893109B (zh) * | 2017-11-08 | 2021-07-06 | 重庆邮电大学 | 一种基于移除野生型序列的低丰度基因突变富集方法 |
KR102086689B1 (ko) * | 2018-02-06 | 2020-03-09 | 주식회사 진씨커 | 돌연변이 세포 유리 유전자 분리 키트 및 이를 이용한 돌연변이 세포 유리 유전자 분리 방법 |
DE202019005627U1 (de) | 2018-04-02 | 2021-05-31 | Grail, Inc. | Methylierungsmarker und gezielte Methylierungssondenpanels |
CN108531603A (zh) * | 2018-05-31 | 2018-09-14 | 青岛普泽麦迪生物技术有限公司 | 一种用于braf基因v600e突变检测的引物对、试剂盒及其检测方法 |
CN113286881A (zh) | 2018-09-27 | 2021-08-20 | 格里尔公司 | 甲基化标记和标靶甲基化探针板 |
WO2020081629A1 (en) * | 2018-10-16 | 2020-04-23 | University Of Cincinnati | Single-molecule epigenetic localization |
CN109880891B (zh) * | 2019-04-22 | 2021-07-30 | 上海交通大学 | 基于核酸酶偶联pcr原理富集低丰度dna突变的检测技术体系及应用 |
CA3145319A1 (en) | 2019-08-21 | 2021-02-25 | Dana-Farber Cancer Institute, Inc. | Multi-site enrichment of deletions in dna microsatellites |
GB2603203A (en) * | 2021-02-02 | 2022-08-03 | Univ Heriot Watt | Method for enriching nucelic acids |
US11993792B2 (en) | 2021-05-27 | 2024-05-28 | New England Biolabs, Inc. | DNase I variants, compositions, methods, and kits |
GB202111195D0 (en) * | 2021-08-03 | 2021-09-15 | Cergentis B V | Method for targeted sequencing |
CN114280128B (zh) * | 2021-12-24 | 2022-11-18 | 清华大学 | 双标记gFET的制备及其在miRNA检测中的应用 |
CN116240200A (zh) * | 2022-07-01 | 2023-06-09 | 中国科学院基础医学与肿瘤研究所(筹) | 一种基于可编程核酸酶的超灵敏目标核酸富集检测方法 |
WO2024084440A1 (en) * | 2022-10-19 | 2024-04-25 | Biofidelity Ltd | Nucleic acid enrichment and detection |
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WO1994002500A1 (en) * | 1992-07-17 | 1994-02-03 | Aprogenex, Inc. | Oligonucleotide probes and primers for detecting chromosomal translocation |
US5629178A (en) | 1994-10-28 | 1997-05-13 | Genetics & Ivf Institute | Method for enhancing amplification in the polymerase chain reaction employing peptide nucleic acid (PNA) |
US6949340B2 (en) * | 2001-03-28 | 2005-09-27 | Creative Mines Llc | Optical phase modulator |
DE10158516A1 (de) | 2001-11-29 | 2003-06-12 | Focusgenomics Gmbh | Verfahren zum Nachweis von Hybridisierungsereignissen in Nukleinsäuren |
AU2002346662A1 (en) * | 2001-12-04 | 2003-06-17 | Euregen Llc | Methods and compositions for selectively cleaving dna containing duplex acids in a complex nucleic acid mixture |
US8440404B2 (en) * | 2004-03-08 | 2013-05-14 | Rubicon Genomics | Methods and compositions for generating and amplifying DNA libraries for sensitive detection and analysis of DNA methylation |
WO2006093082A1 (ja) * | 2005-03-01 | 2006-09-08 | Wako Pure Chemical Industries, Ltd. | サブトラクション・ポリヌクレオチドの取得方法 |
EP1957034A2 (de) * | 2005-11-24 | 2008-08-20 | Basf Se | Chimäre keratinbindende effektorproteine |
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WO2008096146A1 (en) | 2007-02-07 | 2008-08-14 | Solexa Limited | Preparation of templates for methylation analysis |
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US10913977B2 (en) | 2013-07-24 | 2021-02-09 | Dana-Farber Cancer Institute, Inc. | Methods and compositions to enable enrichment of minor DNA alleles by limiting denaturation time in PCR or simply enable enrichment of minor DNA alleles by limiting the denaturation time in PCR |
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WO2015100427A1 (en) | 2013-12-28 | 2015-07-02 | Guardant Health, Inc. | Methods and systems for detecting genetic variants |
ES2917175T3 (es) | 2015-06-24 | 2022-07-07 | Dana Farber Cancer Inst Inc | Degradación selectiva de ADN de tipo salvaje y enriquecimiento de alelos mutantes usando nucleasa |
CA3046953A1 (en) | 2016-12-12 | 2018-06-21 | Dana Farber Cancer Institute, Inc. | Compositions and methods for molecular barcoding of dna molecules prior to mutation enrichment and/or mutation detection |
CA3145319A1 (en) | 2019-08-21 | 2021-02-25 | Dana-Farber Cancer Institute, Inc. | Multi-site enrichment of deletions in dna microsatellites |
-
2016
- 2016-06-24 ES ES16815352T patent/ES2917175T3/es active Active
- 2016-06-24 EP EP16815352.6A patent/EP3313992B1/en active Active
- 2016-06-24 JP JP2017566374A patent/JP6941568B2/ja active Active
- 2016-06-24 CA CA2990846A patent/CA2990846C/en active Active
- 2016-06-24 WO PCT/US2016/039167 patent/WO2016210224A1/en active Application Filing
- 2016-06-24 AU AU2016281718A patent/AU2016281718B2/en active Active
- 2016-06-24 US US15/739,301 patent/US11725230B2/en active Active
-
2018
- 2018-10-17 HK HK18113285.7A patent/HK1254317A1/zh unknown
-
2023
- 2023-08-13 US US18/448,941 patent/US20230392191A1/en active Pending
Also Published As
Publication number | Publication date |
---|---|
CA2990846A1 (en) | 2016-12-29 |
JP6941568B2 (ja) | 2021-09-29 |
JP2018518967A (ja) | 2018-07-19 |
ES2917175T3 (es) | 2022-07-07 |
EP3313992A1 (en) | 2018-05-02 |
US20180187242A1 (en) | 2018-07-05 |
EP3313992A4 (en) | 2018-11-14 |
US20230392191A1 (en) | 2023-12-07 |
AU2016281718A1 (en) | 2017-12-14 |
WO2016210224A1 (en) | 2016-12-29 |
EP3313992B1 (en) | 2022-03-23 |
CA2990846C (en) | 2023-09-26 |
AU2016281718B2 (en) | 2022-03-31 |
US11725230B2 (en) | 2023-08-15 |
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