JP6941568B2 - ヌクレアーゼを使用する野生型dnaの選択的分解および突然変異体対立遺伝子の濃縮 - Google Patents

ヌクレアーゼを使用する野生型dnaの選択的分解および突然変異体対立遺伝子の濃縮 Download PDF

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JP6941568B2
JP6941568B2 JP2017566374A JP2017566374A JP6941568B2 JP 6941568 B2 JP6941568 B2 JP 6941568B2 JP 2017566374 A JP2017566374 A JP 2017566374A JP 2017566374 A JP2017566374 A JP 2017566374A JP 6941568 B2 JP6941568 B2 JP 6941568B2
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ゲラシモス マクリギオルゴス,
ゲラシモス マクリギオルゴス,
チェン ソン,
チェン ソン,
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デイナ ファーバー キャンサー インスティチュート,インコーポレイテッド
デイナ ファーバー キャンサー インスティチュート,インコーポレイテッド
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JP2017566374A 2015-06-24 2016-06-24 ヌクレアーゼを使用する野生型dnaの選択的分解および突然変異体対立遺伝子の濃縮 Active JP6941568B2 (ja)

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US201562183854P 2015-06-24 2015-06-24
US62/183,854 2015-06-24
PCT/US2016/039167 WO2016210224A1 (en) 2015-06-24 2016-06-24 Selective degradation of wild-type dna and enrichment of mutant alleles using nuclease

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Families Citing this family (22)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP6941568B2 (ja) 2015-06-24 2021-09-29 デイナ ファーバー キャンサー インスティチュート,インコーポレイテッド ヌクレアーゼを使用する野生型dnaの選択的分解および突然変異体対立遺伝子の濃縮
CA3046953A1 (en) 2016-12-12 2018-06-21 Dana Farber Cancer Institute, Inc. Compositions and methods for molecular barcoding of dna molecules prior to mutation enrichment and/or mutation detection
WO2019023243A1 (en) 2017-07-24 2019-01-31 Dana-Farber Cancer Institute, Inc. METHODS AND COMPOSITIONS FOR SELECTING AND AMPLIFYING DNA TARGETS IN A SINGLE REACTION MIXTURE
CN107893109B (zh) * 2017-11-08 2021-07-06 重庆邮电大学 一种基于移除野生型序列的低丰度基因突变富集方法
KR102086689B1 (ko) * 2018-02-06 2020-03-09 주식회사 진씨커 돌연변이 세포 유리 유전자 분리 키트 및 이를 이용한 돌연변이 세포 유리 유전자 분리 방법
CN112236520B (zh) 2018-04-02 2025-01-24 格里尔公司 甲基化标记和标靶甲基化探针板
CN108531603A (zh) * 2018-05-31 2018-09-14 青岛普泽麦迪生物技术有限公司 一种用于braf基因v600e突变检测的引物对、试剂盒及其检测方法
CA3111887A1 (en) 2018-09-27 2020-04-02 Grail, Inc. Methylation markers and targeted methylation probe panel
US20210340602A1 (en) * 2018-10-16 2021-11-04 University Of Cincinnati Single-molecule epigenetic localization
CN109880891B (zh) * 2019-04-22 2021-07-30 上海交通大学 基于核酸酶偶联pcr原理富集低丰度dna突变的检测技术体系及应用
AU2020334071A1 (en) 2019-08-21 2022-01-20 Dana-Farber Cancer Institute, Inc. Multi-site enrichment of deletions in DNA microsatellites
EP4121557A4 (en) 2020-08-06 2024-05-01 Singular Genomics Systems, Inc. Spatial sequencing
US11492662B2 (en) 2020-08-06 2022-11-08 Singular Genomics Systems, Inc. Methods for in situ transcriptomics and proteomics
GB2603203A (en) * 2021-02-02 2022-08-03 Univ Heriot Watt Method for enriching nucelic acids
US20240254550A1 (en) * 2021-05-14 2024-08-01 Carmel - Haifa University Economic Corporation Ltd A method of identifying ultra-rare genetic variants
US11993792B2 (en) 2021-05-27 2024-05-28 New England Biolabs, Inc. DNase I variants, compositions, methods, and kits
GB202111195D0 (en) * 2021-08-03 2021-09-15 Cergentis B V Method for targeted sequencing
CN114280128B (zh) * 2021-12-24 2022-11-18 清华大学 双标记gFET的制备及其在miRNA检测中的应用
CN120366267A (zh) * 2022-07-01 2025-07-25 中国科学院基础医学与肿瘤研究所(筹) 一种基于可编程核酸酶的超灵敏目标核酸富集检测方法
EP4605546A1 (en) * 2022-10-19 2025-08-27 Biofidelity Ltd Nucleic acid enrichment and detection
WO2024249961A1 (en) 2023-06-01 2024-12-05 Singular Genomics Systems, Inc. Methods and probes for detecting polynucleotide sequences in cells and tissues
WO2025029475A1 (en) * 2023-07-28 2025-02-06 Guardant Health, Inc. Methods to enrich nucleotide variants by negative selection

Family Cites Families (31)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE69220084T2 (de) 1990-05-07 1997-09-11 Daikin Ind Ltd Diagnostische Anwendungen der Bildung von doppelten D-Loops.
EP0672185A4 (en) * 1992-07-17 1997-04-23 Aprogenex Inc NUCLEIC ACID SAMPLE AND THEIR USE IN DETECTING DOUBLE-STRANDED NUCLEIC ACIDS.
US5629178A (en) 1994-10-28 1997-05-13 Genetics & Ivf Institute Method for enhancing amplification in the polymerase chain reaction employing peptide nucleic acid (PNA)
US6949340B2 (en) * 2001-03-28 2005-09-27 Creative Mines Llc Optical phase modulator
DE10158516A1 (de) 2001-11-29 2003-06-12 Focusgenomics Gmbh Verfahren zum Nachweis von Hybridisierungsereignissen in Nukleinsäuren
AU2002346662A1 (en) * 2001-12-04 2003-06-17 Euregen Llc Methods and compositions for selectively cleaving dna containing duplex acids in a complex nucleic acid mixture
CA2559209C (en) * 2004-03-08 2016-06-07 Rubicon Genomics, Inc. Methods and compositions for generating and amplifying dna libraries for sensitive detection and analysis of dna methylation
EP1854882A4 (en) 2005-03-01 2010-09-22 Wako Pure Chem Ind Ltd METHOD OF OBTAINING A SUBTRACTIVE POLYNUCLEOTIDE
EP1957034A2 (de) * 2005-11-24 2008-08-20 Basf Se Chimäre keratinbindende effektorproteine
WO2007087310A2 (en) 2006-01-23 2007-08-02 Population Genetics Technologies Ltd. Nucleic acid analysis using sequence tokens
WO2008096146A1 (en) 2007-02-07 2008-08-14 Solexa Limited Preparation of templates for methylation analysis
US20080254453A1 (en) 2007-04-12 2008-10-16 Affymetrix, Inc Analysis of methylation using selective adaptor ligation
US7749708B2 (en) * 2007-09-12 2010-07-06 Transgenomic, Inc. Method for identifying the sequence of one or more variant nucleotides in a nucleic acid molecule
WO2010094772A1 (en) * 2009-02-20 2010-08-26 Febit Holding Gmbh Synthesis of sequence-verified nucleic acids
US10636413B2 (en) 2009-06-13 2020-04-28 Rolr, Inc. System for communication skills training using juxtaposition of recorded takes
EP2272976A1 (en) * 2009-07-06 2011-01-12 Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V. Method for differentiation of polynucleotide strands
WO2011057354A1 (en) 2009-11-13 2011-05-19 Commonwealth Scientific And Industrial Research Organisation Epigenetic analysis
CA2792433C (en) 2010-03-08 2016-10-11 Gerassimos Makrigiorgos Full cold-pcr enrichment with reference blocking sequence
US9260753B2 (en) 2011-03-24 2016-02-16 President And Fellows Of Harvard College Single cell nucleic acid detection and analysis
AU2012236896A1 (en) * 2011-03-25 2013-05-16 Integrated Dna Technologies, Inc. RNase H-based assays utilizing modified RNA monomers
EP2691541B1 (en) 2011-03-31 2017-10-18 Dana-Farber Cancer Institute, Inc. Method for enriching in single-stranded mutant sequences from mixture of wild-type and mutant sequences
JP6040227B2 (ja) 2011-05-19 2016-12-07 アジーナ バイオサイエンス, インコーポレイテッド マルチプレックス核酸同定のための製品およびプロセス
US9238836B2 (en) * 2012-03-30 2016-01-19 Pacific Biosciences Of California, Inc. Methods and compositions for sequencing modified nucleic acids
KR102213886B1 (ko) * 2013-03-14 2021-02-05 다카라 바이오 가부시키가이샤 내열성 미스매치 엔도뉴클레아제의 이용방법
CN111383715B (zh) 2013-04-17 2024-09-10 先锋国际良种公司 用于在基因组中表征dna序列组成的方法
WO2015013166A1 (en) * 2013-07-24 2015-01-29 Dana-Farber Cancer Institute, Inc. Methods and compositions to enable enrichment of minor dna alleles by limiting denaturation time in pcr or simply enable enrichment of minor dna alleles by limiting denaturation time in pcr
US9873908B2 (en) 2013-11-27 2018-01-23 Roche Molecular Systems, Inc. Methods for the enrichment of mutated nucleic acid from a mixture
SG11201604923XA (en) 2013-12-28 2016-07-28 Guardant Health Inc Methods and systems for detecting genetic variants
JP6941568B2 (ja) 2015-06-24 2021-09-29 デイナ ファーバー キャンサー インスティチュート,インコーポレイテッド ヌクレアーゼを使用する野生型dnaの選択的分解および突然変異体対立遺伝子の濃縮
CA3046953A1 (en) 2016-12-12 2018-06-21 Dana Farber Cancer Institute, Inc. Compositions and methods for molecular barcoding of dna molecules prior to mutation enrichment and/or mutation detection
AU2020334071A1 (en) 2019-08-21 2022-01-20 Dana-Farber Cancer Institute, Inc. Multi-site enrichment of deletions in DNA microsatellites

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EP3313992A4 (en) 2018-11-14
CA2990846A1 (en) 2016-12-29
US20180187242A1 (en) 2018-07-05
EP3313992B1 (en) 2022-03-23
WO2016210224A1 (en) 2016-12-29
US11725230B2 (en) 2023-08-15
AU2016281718A1 (en) 2017-12-14
CA2990846C (en) 2023-09-26
HK1254317A1 (zh) 2019-07-19
ES2917175T3 (es) 2022-07-07
AU2016281718B2 (en) 2022-03-31
EP3313992A1 (en) 2018-05-02
US20230392191A1 (en) 2023-12-07

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