JP6910145B2 - プライマー伸長によるスライドガラス上での染色 - Google Patents

プライマー伸長によるスライドガラス上での染色 Download PDF

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JP6910145B2
JP6910145B2 JP2016574911A JP2016574911A JP6910145B2 JP 6910145 B2 JP6910145 B2 JP 6910145B2 JP 2016574911 A JP2016574911 A JP 2016574911A JP 2016574911 A JP2016574911 A JP 2016574911A JP 6910145 B2 JP6910145 B2 JP 6910145B2
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JP2017520251A (ja
JP2017520251A5 (enExample
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サムシク，ニコライ
ノーラン，ギャリー，ピー．
ゴルツェフ，ユーリー
マッキルウェーン，デイビッド，ロバート
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Leland Stanford Junior University
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
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    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6818—Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6804—Nucleic acid analysis using immunogens

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JP2016574911A 2014-06-23 2015-06-19 プライマー伸長によるスライドガラス上での染色 Active JP6910145B2 (ja)

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US201462015799P 2014-06-23 2014-06-23
US62/015,799 2014-06-23
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US14/560,921 US9909167B2 (en) 2014-06-23 2014-12-04 On-slide staining by primer extension
PCT/US2015/036763 WO2015200139A1 (en) 2014-06-23 2015-06-19 On-slide staining by primer extension

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Families Citing this family (123)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2766498B1 (en) 2011-10-14 2019-06-19 President and Fellows of Harvard College Sequencing by structure assembly
EP2773779B1 (en) 2011-11-04 2020-10-14 Population Bio, Inc. Methods and compositions for diagnosing, prognosing, and treating neurological conditions
ES2991004T3 (es) 2011-12-22 2024-12-02 Harvard College Métodos para la detección de analitos
US10407724B2 (en) 2012-02-09 2019-09-10 The Hospital For Sick Children Methods and compositions for screening and treating developmental disorders
US10138509B2 (en) 2013-03-12 2018-11-27 President And Fellows Of Harvard College Method for generating a three-dimensional nucleic acid containing matrix
CN111500680B (zh) 2013-06-25 2024-04-16 普罗格诺西斯生物科学公司 检测样品中生物靶标的空间分布的方法和系统
EP2891722B1 (en) 2013-11-12 2018-10-10 Population Bio, Inc. Methods and compositions for diagnosing, prognosing, and treating endometriosis
US9909167B2 (en) 2014-06-23 2018-03-06 The Board Of Trustees Of The Leland Stanford Junior University On-slide staining by primer extension
WO2016007839A1 (en) 2014-07-11 2016-01-14 President And Fellows Of Harvard College Methods for high-throughput labelling and detection of biological features in situ using microscopy
EP4321627A3 (en) 2015-04-10 2024-04-17 10x Genomics Sweden AB Spatially distinguished, multiplex nucleic acid analysis of biological specimens
CN114350752B (zh) 2015-07-17 2025-02-25 布鲁克空间生物学公司 在横切面组织的用户定义区域中的基因表达的同时定量
US10501777B2 (en) 2015-07-17 2019-12-10 Nanostring Technologies, Inc. Simultaneous quantification of a plurality of proteins in a user-defined region of a cross-sectioned tissue
EP4015647B1 (en) 2016-02-26 2023-08-30 The Board of Trustees of the Leland Stanford Junior University Multiplexed single molecule rna visualization with a two-probe proximity ligation system
AU2017257624B2 (en) 2016-04-25 2023-05-25 President And Fellows Of Harvard College Hybridization chain reaction methods for in situ molecular detection
US11512344B2 (en) * 2016-05-10 2022-11-29 Arizona Board Of Regents On Behalf Of Arizona State University Consecutive hybridization for multiplexed analysis of biological samples
JP6996502B2 (ja) * 2016-05-19 2022-01-17 凸版印刷株式会社 標的分子の検出方法
US11352667B2 (en) 2016-06-21 2022-06-07 10X Genomics, Inc. Nucleic acid sequencing
EP3490616B1 (en) 2016-07-27 2022-09-28 The Board of Trustees of the Leland Stanford Junior University Highly-multiplexed fluorescent imaging
GB2569252A (en) 2016-08-31 2019-06-12 Harvard College Methods of combining the detection of biomolecules into a single assay using fluorescent in situ sequencing
JP7093355B2 (ja) 2016-12-16 2022-06-29 アラトム， エルエルシー ライゲーション増幅を使用する分子検出
WO2019068880A1 (en) 2017-10-06 2019-04-11 Cartana Ab RNA MATRIX LIGATION
CN111801350A (zh) * 2018-01-30 2020-10-20 拉什大学医学中心 用于组织和细胞的多重分析的顺序染色
JP7372927B6 (ja) 2018-02-12 2023-11-27 ナノストリング テクノロジーズ，インコーポレイティド 遺伝子およびタンパク質の発現を検出する生体分子プローブおよびその検出方法
WO2019200326A1 (en) 2018-04-13 2019-10-17 Rarecyte, Inc. Kits for labeling of biomarkers and methods of using the same
US11519033B2 (en) 2018-08-28 2022-12-06 10X Genomics, Inc. Method for transposase-mediated spatial tagging and analyzing genomic DNA in a biological sample
WO2020076976A1 (en) 2018-10-10 2020-04-16 Readcoor, Inc. Three-dimensional spatial molecular indexing
WO2020082087A1 (en) 2018-10-19 2020-04-23 Akoya Biosciences, Inc. Detection of co-occurring receptor-coding nucleic acid segments
WO2020092835A1 (en) * 2018-10-31 2020-05-07 Akoya Biosciences, Inc. Methods and kits for detecting cells using oligonucleotide conjugated antibodies
EP3894585A2 (en) 2018-12-10 2021-10-20 10X Genomics, Inc. Generating capture probes for spatial analysis
US12529094B2 (en) 2018-12-10 2026-01-20 10X Genomics, Inc. Imaging system hardware
WO2020139757A1 (en) * 2018-12-27 2020-07-02 Bio-Rad Laboratories, Inc. Sequential multiplex western blotting
US11926867B2 (en) 2019-01-06 2024-03-12 10X Genomics, Inc. Generating capture probes for spatial analysis
US11649485B2 (en) 2019-01-06 2023-05-16 10X Genomics, Inc. Generating capture probes for spatial analysis
ES3056904T3 (en) * 2019-04-25 2026-02-25 Agilent Technologies Inc Hybridization compositions and methods for making and using compositions
EP3976820A1 (en) 2019-05-30 2022-04-06 10X Genomics, Inc. Methods of detecting spatial heterogeneity of a biological sample
CN119220641A (zh) 2019-05-31 2024-12-31 10X基因组学有限公司 检测目标核酸分子的方法
CN114729391A (zh) 2019-09-30 2022-07-08 阿科亚生物科学股份有限公司 用酶介导的放大的多路复用成像
EP4051810B1 (en) * 2019-10-28 2024-08-28 Pixelgen Technologies Ab Method for mapping rolling circle amplification products
EP4025711A2 (en) 2019-11-08 2022-07-13 10X Genomics, Inc. Enhancing specificity of analyte binding
GB201919032D0 (en) 2019-12-20 2020-02-05 Cartana Ab Method of detecting an analyte
GB201919029D0 (en) 2019-12-20 2020-02-05 Cartana Ab Method of detecting an analyte
EP4081656A1 (en) 2019-12-23 2022-11-02 10X Genomics, Inc. Compositions and methods for using fixed biological samples in partition-based assays
CN121022991A (zh) 2019-12-23 2025-11-28 10X基因组学有限公司 使用rna模板化连接进行空间分析的方法
EP4752521A3 (en) 2019-12-23 2026-08-12 10X Genomics, Inc. Reversible fixing reagents and methods of use thereof
US12365942B2 (en) 2020-01-13 2025-07-22 10X Genomics, Inc. Methods of decreasing background on a spatial array
US12405264B2 (en) 2020-01-17 2025-09-02 10X Genomics, Inc. Electrophoretic system and method for analyte capture
US11702693B2 (en) 2020-01-21 2023-07-18 10X Genomics, Inc. Methods for printing cells and generating arrays of barcoded cells
US11732299B2 (en) 2020-01-21 2023-08-22 10X Genomics, Inc. Spatial assays with perturbed cells
US20210230681A1 (en) 2020-01-24 2021-07-29 10X Genomics, Inc. Methods for spatial analysis using proximity ligation
WO2021155063A1 (en) 2020-01-29 2021-08-05 Readcoor, Llc Compositions and methods for analyte detection
US12076701B2 (en) 2020-01-31 2024-09-03 10X Genomics, Inc. Capturing oligonucleotides in spatial transcriptomics
US11898205B2 (en) 2020-02-03 2024-02-13 10X Genomics, Inc. Increasing capture efficiency of spatial assays
US12110541B2 (en) 2020-02-03 2024-10-08 10X Genomics, Inc. Methods for preparing high-resolution spatial arrays
US12110548B2 (en) 2020-02-03 2024-10-08 10X Genomics, Inc. Bi-directional in situ analysis
US11732300B2 (en) 2020-02-05 2023-08-22 10X Genomics, Inc. Increasing efficiency of spatial analysis in a biological sample
US12129516B2 (en) 2020-02-07 2024-10-29 10X Genomics, Inc. Quantitative and automated permeabilization performance evaluation for spatial transcriptomics
US12281357B1 (en) 2020-02-14 2025-04-22 10X Genomics, Inc. In situ spatial barcoding
WO2021167986A1 (en) 2020-02-17 2021-08-26 10X Genomics, Inc. In situ analysis of chromatin interaction
CN116157533A (zh) 2020-02-21 2023-05-23 10X基因组学有限公司 使用杂交方法捕获遗传靶标
EP4107285B1 (en) 2020-02-21 2024-10-09 10X Genomics, Inc. Methods and compositions for integrated in situ spatial assay
US11891654B2 (en) 2020-02-24 2024-02-06 10X Genomics, Inc. Methods of making gene expression libraries
US12188085B2 (en) 2020-03-05 2025-01-07 10X Genomics, Inc. Three-dimensional spatial transcriptomics with sequencing readout
EP4139485B1 (en) 2020-04-22 2023-09-06 10X Genomics, Inc. Methods for spatial analysis using targeted rna depletion
AU2021275906A1 (en) 2020-05-22 2022-12-22 10X Genomics, Inc. Spatial analysis to detect sequence variants
EP4153775B1 (en) 2020-05-22 2024-07-24 10X Genomics, Inc. Simultaneous spatio-temporal measurement of gene expression and cellular activity
US12031177B1 (en) 2020-06-04 2024-07-09 10X Genomics, Inc. Methods of enhancing spatial resolution of transcripts
EP4421186B1 (en) 2020-06-08 2025-08-13 10X Genomics, Inc. Methods of determining a surgical margin and methods of use thereof
US12209273B2 (en) 2020-06-12 2025-01-28 10X Genomics, Inc. Nucleic acid assays using click chemistry bioconjugation
EP4450639B1 (en) 2020-06-25 2025-10-15 10X Genomics, Inc. Spatial analysis of dna methylation
US12209280B1 (en) 2020-07-06 2025-01-28 10X Genomics, Inc. Methods of identifying abundance and location of an analyte in a biological sample using second strand synthesis
US11761038B1 (en) 2020-07-06 2023-09-19 10X Genomics, Inc. Methods for identifying a location of an RNA in a biological sample
US11981960B1 (en) 2020-07-06 2024-05-14 10X Genomics, Inc. Spatial analysis utilizing degradable hydrogels
US12235215B2 (en) 2020-07-20 2025-02-25 Akoya Biosciences, Inc. Processing and imaging tissue samples
US11492662B2 (en) * 2020-08-06 2022-11-08 Singular Genomics Systems, Inc. Methods for in situ transcriptomics and proteomics
US11434525B2 (en) 2020-08-06 2022-09-06 Singular Genomics Systems, Inc. Spatial sequencing
US12297499B2 (en) 2020-08-17 2025-05-13 10X Genomics, Inc. Multicomponent nucleic acid probes for sample analysis
US20220049303A1 (en) 2020-08-17 2022-02-17 Readcoor, Llc Methods and systems for spatial mapping of genetic variants
US11981958B1 (en) 2020-08-20 2024-05-14 10X Genomics, Inc. Methods for spatial analysis using DNA capture
US11926822B1 (en) 2020-09-23 2024-03-12 10X Genomics, Inc. Three-dimensional spatial analysis
US12071667B2 (en) 2020-11-04 2024-08-27 10X Genomics, Inc. Sequence analysis using meta-stable nucleic acid molecules
WO2022109196A1 (en) 2020-11-18 2022-05-27 Akoya Biosciences, Inc. Multiplexed imaging with nanobody probes
US11827935B1 (en) 2020-11-19 2023-11-28 10X Genomics, Inc. Methods for spatial analysis using rolling circle amplification and detection probes
EP4012046A1 (en) 2020-12-11 2022-06-15 10X Genomics, Inc. Methods and compositions for multimodal in situ analysis
WO2022140028A1 (en) 2020-12-21 2022-06-30 10X Genomics, Inc. Methods, compositions, and systems for capturing probes and/or barcodes
US12060603B2 (en) 2021-01-19 2024-08-13 10X Genomics, Inc. Methods for internally controlled in situ assays using padlock probes
EP4738279A3 (en) 2021-02-19 2026-05-13 The Broad Institute Inc. Multi-scale spatial transcriptomics analysis
US12275984B2 (en) 2021-03-02 2025-04-15 10X Genomics, Inc. Sequential hybridization and quenching
EP4305196B1 (en) 2021-04-14 2025-04-02 10X Genomics, Inc. Methods of measuring mislocalization of an analyte
WO2022235764A1 (en) 2021-05-05 2022-11-10 Singular Genomics Systems, Inc. Multiomic analysis device and methods of use thereof
EP4320271B1 (en) 2021-05-06 2025-03-19 10X Genomics, Inc. Methods for increasing resolution of spatial analysis
EP4334471A1 (en) 2021-05-07 2024-03-13 The Broad Institute Inc. Spatiotemporally resolved transcriptomics at subcellular resolution
CN113024665A (zh) * 2021-05-31 2021-06-25 泛肽生物科技(浙江)有限公司 一种oligos标记抗体的方法及其标记得到的抗体
ES3030033T3 (en) 2021-06-03 2025-06-26 10X Genomics Inc Methods, compositions, kits, and systems for enhancing analyte capture for spatial analysis
EP4326900B1 (en) 2021-07-30 2024-09-04 10X Genomics, Inc. Methods and compositions for synchronizing reactions in situ
US12139751B2 (en) 2021-07-30 2024-11-12 10X Genomics, Inc. Circularizable probes for in situ analysis
US12391984B2 (en) 2021-08-03 2025-08-19 10X Genomics, Inc. Compositions and methods for rolling circle amplification
US12529096B2 (en) 2021-08-03 2026-01-20 10X Genomics, Inc. Stabilization and/or compaction of nucleic acid structures
EP4381095A1 (en) 2021-08-03 2024-06-12 10X Genomics, Inc. Nucleic acid concatemers and methods for stabilizing and/or compacting the same
US12460251B2 (en) 2021-08-03 2025-11-04 10X Genomics, Inc. Stabilization and/or compaction of nucleic acid molecules
US12435364B2 (en) 2021-08-16 2025-10-07 10X Genomics, Inc. Probes comprising a split barcode region and methods of use
WO2023034489A1 (en) 2021-09-01 2023-03-09 10X Genomics, Inc. Methods, compositions, and kits for blocking a capture probe on a spatial array
EP4419707A1 (en) 2021-11-10 2024-08-28 10X Genomics, Inc. Methods, compositions, and kits for determining the location of an analyte in a biological sample
EP4305195A2 (en) 2021-12-01 2024-01-17 10X Genomics, Inc. Methods, compositions, and systems for improved in situ detection of analytes and spatial analysis
US12674196B2 (en) 2022-01-18 2026-07-07 10X Genomics, Inc. Methods and compositions for improved probe specificity
EP4733410A3 (en) 2022-01-21 2026-05-27 10X Genomics, Inc. Multiple readout signals for analyzing a sample
WO2023172915A1 (en) 2022-03-08 2023-09-14 10X Genomics, Inc. In situ code design methods for minimizing optical crowding
US12703879B2 (en) 2022-03-29 2026-08-11 10X Genomics, Inc. RNase H assisted in situ rolling circle amplification
CN119032271A (zh) 2022-04-01 2024-11-26 10X基因组学有限公司 用于靶向掩蔽自发荧光的组合物和方法
US12687494B2 (en) 2022-04-04 2026-07-21 10X Genomics, Inc. Systems and methods for nearly isotropic optical resolution using tilted structured illumination microscopy
US12559791B2 (en) 2022-05-06 2026-02-24 10X Genomics, Inc. Methods and compositions for in situ analysis of V(D)J sequences
CN119213143A (zh) 2022-05-11 2024-12-27 10X基因组学有限公司 用于原位测序的组合物和方法
US20240035071A1 (en) 2022-06-17 2024-02-01 10X Genomics, Inc. Catalytic de-crosslinking of samples for in situ analysis
CN119856053A (zh) * 2022-07-20 2025-04-18 哈佛大学校长及研究员协会 高度复用化、热可控的dna延伸方法及其应用
US12692484B2 (en) 2022-08-12 2026-07-28 10X Genomics, Inc. PUMA1 polymerases and uses thereof
US12116626B2 (en) 2022-08-16 2024-10-15 10X Genomics, Inc. AP50 polymerases and uses thereof
EP4685239A1 (en) 2022-11-09 2026-01-28 10X Genomics, Inc. Methods, compositions, and kits for determining the location of multiple analytes in a biological sample
CN116287135A (zh) * 2023-01-12 2023-06-23 华中科技大学 Rna酶抑制剂在rna原位杂交中的用途
EP4720332A1 (en) 2023-06-01 2026-04-08 Singular Genomics Systems, Inc. Methods and probes for detecting polynucleotide sequences in cells and tissues
WO2025029831A1 (en) 2023-07-31 2025-02-06 10X Genomics, Inc. Methods and systems for targeted rna cleavage and target rna-primed rolling circle amplification
EP4560319A1 (en) 2023-11-24 2025-05-28 Pierre Fabre Medicament Immune and tumour cells expression of vista in a panel of cancer indications
WO2025189030A1 (en) 2024-03-07 2025-09-12 Stellaromics, Inc. Methods and systems for volumetric imaging
CN118496347B (zh) * 2024-05-30 2024-10-25 北京航空航天大学杭州创新研究院 一种用于抗体寡核苷酸偶联的方法
CN120005963B (zh) * 2025-02-28 2025-11-07 青岛农业大学 一种快速计数牛奶中菌落总数的方法及染色试剂组合

Family Cites Families (122)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US1000796A (en) 1910-11-08 1911-08-15 Jacob Whitfield Graham Spring-tire.
US5063151A (en) * 1985-09-20 1991-11-05 Biometallics, Inc. Immunoassay method and kit
US5068178A (en) * 1988-12-06 1991-11-26 Genetic Systems Corporation Method of enhancing direct immunofluorescence staining of cells
US5491224A (en) 1990-09-20 1996-02-13 Bittner; Michael L. Direct label transaminated DNA probe compositions for chromosome identification and methods for their manufacture
AU3618593A (en) * 1992-02-04 1993-09-01 E.I. Du Pont De Nemours And Company Amplification of assay reporters by nucleic acid replication
US5985548A (en) * 1993-02-04 1999-11-16 E. I. Du Pont De Nemours And Company Amplification of assay reporters by nucleic acid replication
US5854033A (en) 1995-11-21 1998-12-29 Yale University Rolling circle replication reporter systems
US6027890A (en) 1996-01-23 2000-02-22 Rapigene, Inc. Methods and compositions for enhancing sensitivity in the analysis of biological-based assays
US6117631A (en) * 1996-10-29 2000-09-12 Polyprobe, Inc. Detection of antigens via oligonucleotide antibody conjugates
US20100081134A1 (en) 1997-07-21 2010-04-01 Mirkin Chad A Bio-barcode based detection of target analytes
US5948902A (en) 1997-11-20 1999-09-07 South Alabama Medical Science Foundation Antisense oligonucleotides to human serine/threonine protein phosphatase genes
AU3463099A (en) 1998-03-31 1999-10-18 Tularik Inc. High throughput assay for detection of mrna in cells
US8131053B2 (en) 1999-01-25 2012-03-06 Amnis Corporation Detection of circulating tumor cells using imaging flow cytometry
US6316230B1 (en) 1999-08-13 2001-11-13 Applera Corporation Polymerase extension at 3′ terminus of PNA-DNA chimera
US6998239B1 (en) * 1999-10-12 2006-02-14 Chemocentryx, Inc. Method for identifying a modulator of the binding of CCX CKR polypeptide to a chemokine
WO2001085043A1 (de) 2000-05-11 2001-11-15 Synthes Ag Chur Steckverbindung zur lösbaren verbindung zweier körper, insbesondere pedikelschraube und schraubenzieher
US6511809B2 (en) * 2000-06-13 2003-01-28 E. I. Du Pont De Nemours And Company Method for the detection of an analyte by means of a nucleic acid reporter
US6790636B1 (en) 2000-06-14 2004-09-14 The United States Of America As Represented By The Department Of Health And Human Services Rapid fluorescent labeling of tissue for microdissection using fluorescent specific binding agents
US6531283B1 (en) * 2000-06-20 2003-03-11 Molecular Staging, Inc. Protein expression profiling
US6743592B1 (en) 2000-07-25 2004-06-01 The Trustees Of The University Of Pennsylvania Methods, systems and kits for immuno-detection of epitopes expressed on molecules
US20040038194A1 (en) 2000-10-06 2004-02-26 Hofmann Scott Daniel Diagnostic polymerase chain reaction process utilizing simultaneous capture and detection of amplicons
US20020072053A1 (en) * 2000-12-08 2002-06-13 Mcnally Alan J. Immunoassay based on DNA replication using labeled primer
JP2004532981A (ja) 2001-04-10 2004-10-28 チルドレンズ メディカル センター コーポレーション タンパク質−タンパク質相互作用の解析および標識の方法
EP1270738A1 (de) 2001-06-18 2003-01-02 chimera biotec GmbH Verfahren zum Nachweis von Substanzen in Flüssigkeiten
US20020197694A1 (en) 2001-06-20 2002-12-26 Weiping Shao Conjugates of reduced antibodies and biomolecules
US7341831B2 (en) 2001-07-18 2008-03-11 The Trustees Of The University Of Pennsylvania Method for immuno-detection of epitopes
US20040091857A1 (en) * 2001-07-20 2004-05-13 Nallur Girish N. Gene expression profiling
US6658780B2 (en) 2001-09-06 2003-12-09 Aris Papadimas Revolver device
JP3926178B2 (ja) * 2001-11-02 2007-06-06 富士通株式会社 蛋白質検出デバイス
AU2003209340A1 (en) * 2002-01-18 2003-09-02 Bristol-Myers Squibb Company Predictor sets for tyrosine kinase pathways
EP1490514A4 (en) 2002-03-29 2008-12-17 Nugen Technologies Inc THROUGH ISOTHERME SINGLE PRIMER NUCLEIC ACID AMPLIFICATION IMPROVED DETECTION AND QUANTIFICATION OF ANALYTES
WO2004074503A2 (en) * 2003-02-21 2004-09-02 Hoser Mark J Nucleic acid sequencing methods, kits and reagents
US20040185453A1 (en) * 2003-03-21 2004-09-23 Joel Myerson Affinity based methods for separating homologous parental genetic material and uses thereof
TWI375796B (en) * 2003-04-18 2012-11-01 Becton Dickinson Co Immuno-amplification
US7198900B2 (en) 2003-08-29 2007-04-03 Applera Corporation Multiplex detection compositions, methods, and kits
US7341837B2 (en) * 2003-09-02 2008-03-11 Lawton Robert L Soluble analyte detection and amplification
WO2005054514A1 (en) * 2003-12-02 2005-06-16 Agency For Science, Technology And Research Immuno-amplification rna assay
KR100695123B1 (ko) 2003-12-03 2007-03-14 삼성전자주식회사 Ｔｍ에 따라 기판상에 고정화된 프로브 폴리뉴클레오티드군을 2이상 포함하는 폴리뉴클레오티드 어레이 및 그를이용하는 표적 핵산 검출 방법
US20050233340A1 (en) 2004-04-20 2005-10-20 Barrett Michael T Methods and compositions for assessing CpG methylation
CN101137758B (zh) 2004-11-03 2012-10-10 意力速分子诊断股份有限公司 均相分析物检测
CA2598508A1 (fr) 2005-02-25 2006-08-31 Commissariat A L'energie Atomique Procede et dispositif pour separer des cibles moleculaires dans un melange complexe
US20070020650A1 (en) * 2005-04-01 2007-01-25 Avak Kahvejian Methods for detecting proteins
CA2609328A1 (en) 2005-05-26 2006-11-30 The Trustees Of Boston University Quantification of nucleic acids and proteins using oligonucleotide mass tags
US20070026430A1 (en) 2005-06-30 2007-02-01 Applera Corporation Proximity probing of target proteins comprising restriction and/or extension
US8530196B2 (en) 2006-04-07 2013-09-10 The Trustees Of The Univeristy Of Pennsylvania In situ cloning from pathological tissue specimens
WO2008005673A2 (en) 2006-06-30 2008-01-10 Applera Corporation Reversible terminator nucleotides and methods of use
US20080124735A1 (en) * 2006-10-16 2008-05-29 Matthias Schuster Method for detection of one or more CpG positions
WO2008052774A2 (en) 2006-10-31 2008-05-08 Noxxon Pharma Ag Methods for detection of a single- or double-stranded nucleic acid molecule
US8305579B2 (en) 2006-11-16 2012-11-06 Thomas Pirrie Treynor Sequential analysis of biological samples
US9201063B2 (en) 2006-11-16 2015-12-01 General Electric Company Sequential analysis of biological samples
SE531135C2 (sv) 2006-12-01 2008-12-23 Alf Maansson Känslig detektion av gifter, patogener och sjukdomsmarkörer genom aggregation av cytoskeletala filament
WO2008076375A2 (en) 2006-12-13 2008-06-26 Autogenomics, Inc. Concurrent analysis of multiple patient samples using solid phase addressable multiplex test with high signal-to-noise ratio
CN101210270A (zh) 2006-12-31 2008-07-02 陕西北美基因股份有限公司 病毒病原高通量快速排查检测方法
GB0703996D0 (en) * 2007-03-01 2007-04-11 Oxitec Ltd Nucleic acid detection
US8003314B2 (en) 2007-04-16 2011-08-23 Diagnostic Hybrids, Inc. Methods for direct fluorescent antibody virus detection in liquids
WO2009012220A2 (en) * 2007-07-17 2009-01-22 Agilent Technologies Inc. Analyte detection assays
US8088715B2 (en) 2007-11-13 2012-01-03 Ikonisys, Inc. Detection of circulating tumor cells in peripheral blood with an automated scanning fluorescence microscope
WO2009126678A2 (en) 2008-04-08 2009-10-15 Cornell University Detection of target nucleic acid sequences using fluorescence resonance energy transfer
US20100021904A1 (en) 2008-05-21 2010-01-28 Pierce Niles A Shielded cross-linking probes
US8530156B2 (en) 2008-08-08 2013-09-10 President And Fellows Of Harvard College Chemically cleavable phosphoramidite linkers for sequencing by ligation
US8685937B2 (en) 2008-08-09 2014-04-01 University Of Iowa Research Foundation Nucleic acid aptamers
US8262879B2 (en) 2008-09-03 2012-09-11 Nabsys, Inc. Devices and methods for determining the length of biopolymers and distances between probes bound thereto
US9156010B2 (en) 2008-09-23 2015-10-13 Bio-Rad Laboratories, Inc. Droplet-based assay system
US9089844B2 (en) 2010-11-01 2015-07-28 Bio-Rad Laboratories, Inc. System for forming emulsions
US8309306B2 (en) 2008-11-12 2012-11-13 Nodality, Inc. Detection composition
EP2350657A4 (en) 2008-11-21 2013-06-12 Univ Saint Louis BIOSENSOR FOR DETECTING MULTIPLE EPITOPES ON ONE TARGET
DE102008062372B3 (de) 2008-12-17 2010-06-17 Medizinische Hochschule Hannover Nachweiskonjugat und Verfahren zur Analyse
WO2010115089A2 (en) 2009-04-03 2010-10-07 University Of Virginia Patent Foundation Serial multiple antigen colocalization in paraffin-embedded tissue
US9284559B2 (en) 2009-04-14 2016-03-15 Wake Forest University Health Sciences Multivalent aptamer complexes
WO2010129666A1 (en) 2009-05-05 2010-11-11 Altermune Technologies, Llc Chemically programmable immunity
US9677125B2 (en) 2009-10-21 2017-06-13 General Electric Company Detection of plurality of targets in biological samples
WO2011143583A1 (en) * 2010-05-13 2011-11-17 Illumina, Inc. Binding assays for markers
US8658780B2 (en) 2010-05-18 2014-02-25 California Institute Of Technology Triggered covalent probes for imaging and silencing genetic expression
US20120004132A1 (en) 2010-07-02 2012-01-05 Affymetrix, Inc. Detection of Nucleic Acids and Proteins
WO2012018397A2 (en) 2010-08-02 2012-02-09 The Regents Of The University Of California Molecular subtyping of oral squamous cell carcinoma to distinguish a subtype that is unlikely to metastasize
US20120034603A1 (en) 2010-08-06 2012-02-09 Tandem Diagnostics, Inc. Ligation-based detection of genetic variants
WO2012058638A2 (en) 2010-10-29 2012-05-03 President And Fellows Of Harvard College Nucleic acid nanostructure barcode probes
EP2633081B1 (en) * 2010-10-29 2017-01-11 Olink Bioscience AB Proximity ligation technology for western blot applications
US20120258870A1 (en) 2010-11-22 2012-10-11 The University Of Chicago Methods, Systems, and/or Use of Oligonucleotide Conjugates to Develop Panels for Use in Assays and Detections
US20120178081A1 (en) 2010-12-31 2012-07-12 Affymetrix. Inc. Methods of Labeling Cells, Labeled Cells, and uses Thereof
US20120196294A1 (en) * 2011-01-17 2012-08-02 Life Technologies Corporation Workflow for detection of ligands using nucleic acids
GB201101621D0 (en) * 2011-01-31 2011-03-16 Olink Ab Method and product
US20120252682A1 (en) 2011-04-01 2012-10-04 Maples Corporate Services Limited Methods and systems for sequencing nucleic acids
US20140170653A1 (en) * 2011-04-15 2014-06-19 Life Technologies Corporation Chemical ligation
US8946389B2 (en) 2011-04-25 2015-02-03 University Of Washington Compositions and methods for multiplex biomarker profiling
GB201107863D0 (en) 2011-05-11 2011-06-22 Olink Ab Method and product
GB201108678D0 (en) * 2011-05-24 2011-07-06 Olink Ab Multiplexed proximity ligation assay
US9938524B2 (en) * 2011-11-22 2018-04-10 Active Motif, Inc. Multiplex isolation of protein-associated nucleic acids
US10689643B2 (en) * 2011-11-22 2020-06-23 Active Motif, Inc. Targeted transposition for use in epigenetic studies
EP2794928B1 (en) * 2011-12-22 2019-02-20 President and Fellows of Harvard College Compositions and methods for analyte detection
GB201201547D0 (en) 2012-01-30 2012-03-14 Olink Ab Method and product
US9267168B2 (en) 2012-06-12 2016-02-23 Pacific Biosciences Of California, Inc. Methods and compositions for isolating template nucleic acids
WO2013188756A1 (en) 2012-06-15 2013-12-19 The University Of Chicago Oligonucleotide-mediated quantitative multiplexed immunoassays
US9707562B2 (en) 2013-03-13 2017-07-18 Denovo Sciences, Inc. System for capturing and analyzing cells
EP2971141B1 (en) 2013-03-15 2018-11-28 Genapsys, Inc. Systems for biological analysis
WO2014182283A1 (en) 2013-05-07 2014-11-13 Empire Technology Development Llc Terahertz frequency tags and methods for their preparation and use
JP6600302B2 (ja) 2013-06-12 2019-10-30 ザ ジェネラル ホスピタル コーポレイション 標的分子のマルチプレックス検出のための方法、キット、およびシステム、ならびにそれらの使用
CN105555972B (zh) * 2013-07-25 2020-07-31 伯乐生命医学产品有限公司 遗传测定
US20160161472A1 (en) 2013-07-30 2016-06-09 President And Fellows Of Harvard College Quantitative dna-based imaging and super-resolution imaging
US9193359B2 (en) 2013-08-12 2015-11-24 GM Global Technology Operations LLC Vehicle systems and methods for identifying a driver
CN105764490B (zh) * 2013-09-24 2020-10-09 加利福尼亚大学董事会 用于生物测定和诊断的胶囊封装的传感器和感测系统及其制造和使用方法
AU2014333815B2 (en) 2013-10-11 2020-07-09 Nihon University School Of Medicine Multiplex HER2 and estrogen receptor co-staining assays for detecting tumor heterogeneity
US9834814B2 (en) 2013-11-22 2017-12-05 Agilent Technologies, Inc. Spatial molecular barcoding of in situ nucleic acids
GB201401885D0 (en) 2014-02-04 2014-03-19 Olink Ab Proximity assay with detection based on hybridisation chain reaction (HCR)
EP3117214B1 (en) 2014-03-11 2020-05-06 President and Fellows of Harvard College High-throughput and highly multiplexed imaging with programmable nucleic acid probes
US9753036B2 (en) * 2014-04-29 2017-09-05 Edp Biotech Corporation Methods and compositions for screening and detecting biomarkers
EP3155131B1 (en) 2014-06-10 2020-02-12 Blueprint Medicines Corporation Raf1 fusions
EP4270006A3 (en) 2014-06-13 2024-01-10 Immudex ApS General detection and isolation of specific cells by binding of labeled molecules
US9909167B2 (en) 2014-06-23 2018-03-06 The Board Of Trustees Of The Leland Stanford Junior University On-slide staining by primer extension
CN106604933B (zh) 2014-07-11 2021-08-10 文塔纳医疗系统公司 抗pd-l1抗体及其诊断用途
US9625387B2 (en) 2014-09-16 2017-04-18 Lawrence Livermore National Security, Llc System and method for controlling depth of imaging in tissues using fluorescence microscopy under ultraviolet excitation following staining with fluorescing agents
US10071121B2 (en) 2014-11-14 2018-09-11 San Diego State University (Sdsu) Foundation Cardiac, mesenchymal and endothelial progenitor cell (CPC) chimeras and methods for making and using them
WO2016085659A1 (en) 2014-11-24 2016-06-02 Battelle Memorial Institute Chromatin activity precipitation method and system
US10006917B2 (en) 2014-12-15 2018-06-26 President And Fellows Of Harvard College Methods and compositions relating to super-resolution imaging and modification
US20180095067A1 (en) 2015-04-03 2018-04-05 Abbott Laboratories Devices and methods for sample analysis
US20180057873A1 (en) 2015-04-17 2018-03-01 Centrillion Technology Holdings Corporation Methods for performing spatial profiling of biological materials
CN114350752B (zh) 2015-07-17 2025-02-25 布鲁克空间生物学公司 在横切面组织的用户定义区域中的基因表达的同时定量
KR102557419B1 (ko) 2015-08-07 2023-07-19 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 단백질-단백질 상호작용의 초해상도 영상화
EP3458858B1 (en) 2016-05-15 2022-07-06 Ultivue, Inc. Multiplexed imaging using strand displacement
US20170349949A1 (en) 2016-06-03 2017-12-07 Partikula Llc Methods of selecting subjects for treatment with metabolic modulators
EP3490616B1 (en) 2016-07-27 2022-09-28 The Board of Trustees of the Leland Stanford Junior University Highly-multiplexed fluorescent imaging
US11099170B2 (en) 2018-06-13 2021-08-24 The Board Of Trustees Of The University Of Illinois Parallel DNA detection by solid-state multi-pore systems and associated methods

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