JP6871158B2 - 植物体におけるニコチンからノルニコチンへの変換の低減 - Google Patents

植物体におけるニコチンからノルニコチンへの変換の低減 Download PDF

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JP6871158B2
JP6871158B2 JP2017510762A JP2017510762A JP6871158B2 JP 6871158 B2 JP6871158 B2 JP 6871158B2 JP 2017510762 A JP2017510762 A JP 2017510762A JP 2017510762 A JP2017510762 A JP 2017510762A JP 6871158 B2 JP6871158 B2 JP 6871158B2
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nicotine demethylase
plant
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mutation
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JP2017519519A (ja
JP2017519519A5 (https=
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ヴェレーナ リードシュルテ
ヴェレーナ リードシュルテ
シモン ゲプフェルト
シモン ゲプフェルト
ルシアン ボヴェ
ルシアン ボヴェ
ニコラス シエロ
ニコラス シエロ
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フィリップ・モーリス・プロダクツ・ソシエテ・アノニム
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8218—Antisense, co-suppression, viral induced gene silencing [VIGS], post-transcriptional induced gene silencing [PTGS]
    • C—CHEMISTRY; METALLURGY
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C—CHEMISTRY; METALLURGY
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0071—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
    • C12N9/0073—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14) with NADH or NADPH as one donor, and incorporation of one atom of oxygen 1.14.13
    • C—CHEMISTRY; METALLURGY
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/6895—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for plants, fungi or algae
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/13—Plant traits
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/156—Polymorphic or mutational markers
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    • C12Y—ENZYMES
    • C12Y114/00—Oxidoreductases acting on paired donors, with incorporation or reduction of molecular oxygen (1.14)
    • C12Y114/13—Oxidoreductases acting on paired donors, with incorporation or reduction of molecular oxygen (1.14) with NADH or NADPH as one donor, and incorporation of one atom of oxygen (1.14.13)

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  • Health & Medical Sciences (AREA)
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  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Plant Pathology (AREA)
  • Analytical Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Nutrition Science (AREA)
  • Medicinal Chemistry (AREA)
  • Virology (AREA)
  • Botany (AREA)
  • Mycology (AREA)
  • Immunology (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Manufacture Of Tobacco Products (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
JP2017510762A 2014-05-08 2015-05-07 植物体におけるニコチンからノルニコチンへの変換の低減 Expired - Fee Related JP6871158B2 (ja)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
EP14167598.3 2014-05-08
EP14167598 2014-05-08
EP14001645 2014-05-09
EP14001645.2 2014-05-09
PCT/EP2015/060124 WO2015169927A1 (en) 2014-05-08 2015-05-07 Reduction of nicotine to nornicotine conversion in plants

Publications (3)

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JP2017519519A JP2017519519A (ja) 2017-07-20
JP2017519519A5 JP2017519519A5 (https=) 2018-06-14
JP6871158B2 true JP6871158B2 (ja) 2021-05-12

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JP2017510762A Expired - Fee Related JP6871158B2 (ja) 2014-05-08 2015-05-07 植物体におけるニコチンからノルニコチンへの変換の低減

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US (1) US10415050B2 (https=)
EP (1) EP3140404A1 (https=)
JP (1) JP6871158B2 (https=)
KR (1) KR102442802B1 (https=)
CN (1) CN106459923B (https=)
BR (1) BR112016025234A2 (https=)
CA (1) CA2944965A1 (https=)
MX (1) MX2016014635A (https=)
PH (1) PH12016501718A1 (https=)
RU (1) RU2733837C2 (https=)
WO (1) WO2015169927A1 (https=)

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CN106636146B (zh) * 2017-02-16 2019-09-13 云南省烟草农业科学研究院 一种降低尼古丁转化率的cyp82e5基因突变体及其应用
US11046969B2 (en) * 2017-05-24 2021-06-29 Epiplanta Biotech Ltd. Transgenic plant and the method for producing the same
US20200140875A1 (en) * 2017-05-31 2020-05-07 22Nd Century Limited, Llc Genome editing methods for producing low-nicotine tobacco products
CN111726983B (zh) 2017-12-07 2024-05-07 肯塔基大学研究基金会 bZIP转录因子调节烟碱向降烟碱的转化
CN108192900B (zh) * 2018-01-16 2021-11-30 西南大学 烟草烟碱去甲基化酶基因cyp82e4的编辑失活载体及其应用
AR115022A1 (es) 2018-03-28 2020-11-18 Philip Morris Products Sa Modulación del contenido de aminoácidos en una planta
AR115023A1 (es) 2018-03-28 2020-11-18 Philip Morris Products Sa Modulación del contenido de azúcar reductor en una planta
CN108424921B (zh) * 2018-04-20 2021-04-20 云南省烟草农业科学研究院 一种降低尼古丁转化率的cyp82e10基因错义突变体m594及其应用
EP3827086A1 (en) * 2018-07-26 2021-06-02 Altria Client Services LLC Compositions and methods based on pmt engineering for producing tobacco plants and products having altered alkaloid levels
GB201812603D0 (en) 2018-08-02 2018-09-19 British American Tobacco Investments Ltd Method
EP3830251A1 (en) * 2018-08-02 2021-06-09 Antidote Therapeutics, Inc. Novel nicotine degrading enzyme variants
US11976286B2 (en) 2018-12-30 2024-05-07 Philip Morris Products S.A. Modulation of nitrate levels in plants via mutation of nitrate reductase
JP2022552638A (ja) 2019-10-01 2022-12-19 フィリップ・モーリス・プロダクツ・ソシエテ・アノニム 植物における糖およびアミノ酸含有量の調節（ｓｕｌｔｒ３）
JP7659547B2 (ja) 2019-10-01 2025-04-09 フィリップ・モーリス・プロダクツ・ソシエテ・アノニム 植物における還元糖含有量の調節（ｉｎｖ）
CN114981440A (zh) * 2019-12-03 2022-08-30 奥驰亚客户服务有限公司 用于产生具有改变的生物碱水平的烟草植物和制品的方法及其组合物
US11331020B2 (en) 2020-02-06 2022-05-17 Trustees Of Boston University Enzyme-based electrochemical nicotine biosensor
US11536685B2 (en) 2020-02-06 2022-12-27 Trustees Of Boston University High throughput assay for identifying microbial redox enzymes
JP7210806B2 (ja) * 2020-04-17 2023-01-23 日本たばこ産業株式会社 低アルカロイド含量のタバコ属植物体およびその製造方法
CN111505158B (zh) * 2020-05-09 2022-09-23 云南省烟草农业科学研究院 一种基于孵化处理的尼古丁转化烟株色质快速筛查方法
US20240368615A1 (en) 2021-09-10 2024-11-07 Philip Morris Products S.A. Modulating alkaloid profiles in nicotiana tabacum
CN113980978B (zh) * 2021-11-22 2023-12-26 云南中烟工业有限责任公司 一种烟草烟碱转运蛋白相关的基因及其应用
WO2023117661A1 (en) 2021-12-20 2023-06-29 Philip Morris Products S.A. Increasing anatabine in tobacco leaf by regulating methyl putrescine oxidase
WO2023117701A1 (en) 2021-12-21 2023-06-29 Philip Morris Products S.A. Modulation of nicotine production by alteration of nicotinamidase expression or function in plants
CN114540382B (zh) * 2022-02-18 2023-07-18 贵州省烟草科学研究院 烟草镉转运基因NtPLA1及应用
JP2025533986A (ja) 2022-10-13 2025-10-09 フィリップ・モーリス・プロダクツ・ソシエテ・アノニム Ｎｔｐ２を調節することによって葉バイオマスおよび窒素利用効率を増加させる
EP4658670A1 (en) 2023-02-02 2025-12-10 Philip Morris Products S.A. Modulation of genes coding for lysine ketoglutarate reductase
JP2026506774A (ja) 2023-02-02 2026-02-26 フィリップ・モーリス・プロダクツ・ソシエテ・アノニム 糖トランスポーターの調節
CN121335615A (zh) 2023-06-29 2026-01-13 菲利普莫里斯生产公司 编码谷氨酸脱氢酶的基因的调节

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US10415050B2 (en) 2019-09-17
PH12016501718A1 (en) 2016-11-07
CN106459923A (zh) 2017-02-22
JP2017519519A (ja) 2017-07-20
KR102442802B1 (ko) 2022-09-14
BR112016025234A2 (pt) 2017-12-12
CA2944965A1 (en) 2015-11-12
KR20170002444A (ko) 2017-01-06
RU2733837C2 (ru) 2020-10-07
RU2016144409A3 (https=) 2018-11-29
US20170130239A1 (en) 2017-05-11
RU2016144409A (ru) 2018-06-13
WO2015169927A1 (en) 2015-11-12
EP3140404A1 (en) 2017-03-15
MX2016014635A (es) 2017-02-23
CN106459923B (zh) 2020-08-11

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