JP2021519098A - 植物におけるアミノ酸含有量の調節 - Google Patents
植物におけるアミノ酸含有量の調節 Download PDFInfo
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Classifications
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- A24B—MANUFACTURE OR PREPARATION OF TOBACCO FOR SMOKING OR CHEWING; TOBACCO; SNUFF
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- A—HUMAN NECESSITIES
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- A24B—MANUFACTURE OR PREPARATION OF TOBACCO FOR SMOKING OR CHEWING; TOBACCO; SNUFF
- A24B15/00—Chemical features or treatment of tobacco; Tobacco substitutes, e.g. in liquid form
- A24B15/18—Treatment of tobacco products or tobacco substitutes
- A24B15/28—Treatment of tobacco products or tobacco substitutes by chemical substances
- A24B15/30—Treatment of tobacco products or tobacco substitutes by chemical substances by organic substances
- A24B15/302—Treatment of tobacco products or tobacco substitutes by chemical substances by organic substances by natural substances obtained from animals or plants
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- C—CHEMISTRY; METALLURGY
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- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
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Abstract
Description
有利なことに、NtAATポリヌクレオチド配列は、乾燥処理中に、特に乾燥処理の開始から高度に発現することができる。1つ以上のNtAATポリヌクレオチド配列の発現を調節することにより、アスパラギン酸のレベルを乾燥処理プロセス全体を通して調節することができるため、エアロゾルにおけるアクリルアミドのレベルの調節をもたらすことができる。特に、1つ以上のNtAATポリヌクレオチド配列の発現を減少させることにより、エアロゾルにおけるアクリルアミドのレベルの減少をもたらすことができる。
「遺伝子構築物」とは、ポリペプチドをコードするポリヌクレオチドを含む、DNAまたはRNA分子を指す。コード配列は、発現を導くことが可能なプロモーターおよびポリアデニル化シグナルを含む制御要素に動作可能に連結される開始および終結シグナルを含むことができる。
一実施形態では、配列表に示される任意のポリヌクレオチドを含む、本明細書に説明される任意の配列に対して少なくとも60%の配列同一性を有する配列を含むか、それからなるか、またはそれから本質的になる単離ポリヌクレオチドが提供される。好適には、単離ポリヌクレオチドは、それに対して少なくとも60%、61%、62%、63%、64%、65%、66%、67%、68%、69%、70%、75%、80%、85%、87%、88%、89%、90%、91%、92%、93%、94%、95%、96%、97%、98%、99%、もしくは100%の配列同一性を有する配列を含むか、それからなるか、またはそれから本質的になる。好適には、本明細書に説明されるポリヌクレオチド(複数可)は、配列表に示されるポリペプチド(複数可)の機能または活性の少なくとも約50%、60%、70%、80%、90%、95%、96%、97%、98%、99%、100%以上を有する活性AATポリペプチドをコードする。
別の態様では、配列表に示される任意のポリペプチドを含む、本明細書に説明される任意のポリペプチドに対して少なくとも60%の配列同一性を有するポリペプチドを含むか、それからなるか、またはそれから本質的になる単離ポリペプチドが提供される。好適には、単離ポリペプチドは、それに対して少なくとも60%、61%、62%、63%、64%、65%、66%、67%、68%、69%、70%、75%、80%、85%、87%、88%、89%、90%、91%、92%、93%、94%、95%、96%、97%、98%、99%、99.1%、99.2%、99.3%、99.4%、99.5%、99.6%、99.7%、99.8%、99.9%、もしくは100%の配列同一性を有する配列を含むか、それからなるか、またはそれから本質的になる。
a.形質転換
組み換え構築物は、ポリペプチドの発現、機能、または活性を調節するために、植物または植物細胞を形質転換するために使用することができる。組み換えポリヌクレオチド構築物は、1つ以上の本明細書に説明されるポリヌクレオチドをコードし、ポリペプチドを発現するのに好適な制御領域に動作可能に連結されたポリヌクレオチドを含むことができる。したがって、ポリヌクレオチドは、本明細書に説明されるポリペプチドをコードするコード配列を含むことができる。ポリペプチドの発現、機能、または活性が調節されている植物または植物細胞としては、突然変異型、非天然型、トランスジェニック、人造、または遺伝子組み換え植物または植物細胞を挙げることができる。好適には、トランスジェニック植物または植物細胞は、組み換えDNAの安定した組み込みによって変更されたゲノムを含む。組み換えDNAは、遺伝子組み換えされ、細胞の外部に構築されたDNAを含み、かつ天然型DNAもしくはcDNA、または合成DNAを含有するDNAを含む。トランスジェニック植物は、最初に形質転換された植物細胞から再生された植物や、形質転換された植物の後の世代または交雑種からの子孫トランスジェニック植物を含むことができる。好適には、トランスジェニック改変は、対照植物と比較して、ポリヌクレオチドまたは本明細書に説明されるポリペプチドの発現または機能または活性を変更する。
本明細書に説明される1つ以上のポリヌクレオチドまたはポリペプチドにおける突然変異を含む植物または植物細胞が開示され、該突然変異は、調節されたAAT機能または活性をもたらす。説明される突然変異は別として、突然変異型植物または植物細胞は、本明細書に説明されるものと同一のポリヌクレオチドもしくはポリペプチドにおいて、またはゲノム内の1つ以上の他のポリヌクレオチドまたはポリペプチドにおいて、のいずれかで、1つ以上のさらなる突然変異を有することができる。
突然変異誘発以外に、本明細書に説明されるポリヌクレオチドまたはポリペプチドのうちの1つ以上の発現または機能または活性を調節することができる組成物としては、1つ以上の内在性遺伝子(複数可)の転写を妨害できる配列特異的ポリヌクレオチド、RNA転写物(例えば、二本鎖RNA、siRNA、リボザイム)の翻訳を妨害できる配列特異的ポリヌクレオチド、1つ以上のポリペプチドの安定性を妨害できる配列特異的ポリペプチド、1つ以上のポリペプチドの酵素機能または基質もしくは調節ポリペプチドに関する1つ以上のポリペプチドの結合機能を妨害できる配列特異的ポリヌクレオチド、1つ以上のポリペプチドに対して特異性を示す抗体、1つ以上のポリペプチドの安定性または1つ以上のポリペプチドの酵素機能または1つ以上のポリペプチドの結合機能を妨害できる小分子化合物、1つ以上のポリヌクレオチドを結合するジンクフィンガーポリペプチド、および1つ以上のポリヌクレオチドに対して機能を有するメガヌクレアーゼが挙げられる。遺伝子編集技術、遺伝的編集技術、およびゲノム編集技術は、当該技術分野で周知である。
ジンクフィンガーポリペプチドを使用して、1つ以上の本明細書に説明されるポリヌクレオチドの発現または機能または活性を調節することができる。様々な実施形態では、ポリヌクレオチドのコード配列の一部またはすべてを含むゲノムDNA配列は、ジンクフィンガーヌクレアーゼ媒介突然変異誘発によって改変される。ゲノムDNA配列で、ジンクフィンガーポリペプチド結合のための一意の部位が検索される。あるいは、ゲノムDNA配列で、ジンクフィンガーポリペプチド結合のための2つの一意の部位が検索されるが、ここで、両方の部位は対向する鎖上にあり、例えば、1、2、3、4、5、6つ以上の塩基対だけ離れた互いに近い場所にある。したがって、ポリヌクレオチドに結合するジンクフィンガーポリペプチドが提供される。
別の態様では、I−CreIなどのメガヌクレアーゼを使用して、突然変異型、非天然型、またはトランスジェニック、あるいは遺伝子組み換え植物を生成するための方法が説明される。天然型メガヌクレアーゼならびに組み換えメガヌクレアーゼを使用して、植物のゲノムDNA内の単一の部位で、または比較的少ない部位で、特異的に二本鎖切断を引き起こし、1つ以上の本明細書に説明されるポリヌクレオチドの分裂を可能にすることができる。メガヌクレアーゼは、変更されたDNA認識特性を有する操作されたメガヌクレアーゼであり得る。メガヌクレアーゼポリペプチドは、当該技術分野において既知の様々な異なる機構によって、植物細胞に送達することができる。
遺伝子編集の1つの方法には、細胞が修復機構を用いて応答できる二本鎖の分解を誘導する、転写活性化因子様エフェクターヌクレアーゼ(TALEN)の使用が関与する。NHEJは、アニーリングのための配列重複が非常に少ないかまたは全くない二本鎖切断のうちのいずれかの側からDNAを再結合する。この修復機構は、挿入もしくは欠失、または染色体の再配列によるゲノム内のエラーを誘発する。このようなエラーがあると、遺伝子産物はその位置で非機能性にコードされ得る。特定の用途では、ポリヌクレオチドを植物のゲノムから正確に除去することが望ましい場合がある。このような用途は、操作されたメガヌクレアーゼの対を使用して可能であり、その各々が意図された欠失のいずれかの側にあるメガヌクレアーゼ認識部位を切断する。また、遺伝子を認識してそれに結合し、二本鎖切断をゲノムに導入することができるTALENも使用することができる。したがって、別の態様では、TALエフェクターヌクレアーゼを使用して、本明細書に説明されるような突然変異型、非天然型、またはトランスジェニック、あるいは遺伝子組み換え植物を生成するための方法が企図される。
遺伝子編集の別の方法には、細菌性CRISPR/Casシステムの使用が関与する。細菌および古細菌は、ウイルスおよびプラスミドDNAの侵入から保護する適応免疫系の一部である、クラスター化され規則的に間隔が空いた短い回文の反復(CRISPR)と呼ばれる染色体要素を示す。II型CRISPRシステムでは、CRISPR RNA(crRNA)がトランス活性化crRNA(tracrRNA)およびCRISPR関連(Cas)ポリペプチドで機能し、標的DNA中に二本鎖切断を導入する。Cas9による標的切断は、crRNAとtracrRNAとの間の塩基対合、およびcrRNAと標的DNAとの間の塩基対合を必要とする。標的認識は、配列NGGと一致する、プロトスペーサー隣接モチーフ(PAM)と呼ばれる短いモチーフの存在によって促進される。このシステムは、ゲノム編集のために利用できる。Cas9は通常、crRNAおよびtracrRNAで構成される二重RNAによってプログラムされる。しかしながら、これらのRNAのコア構成要素は、Cas9標的化のために単一の混成体「ガイドRNA」に組み合わせることができる。部位特異的切断のためにDNAを標的化する非コードRNAガイドの使用は、TALENなどの既存の技術よりもはるかに簡単であることが約束される。CRISPR/Cas戦略を使用して、ヌクレアーゼ複合体を再標的化するには、新しいRNA配列の導入のみを必要とし、ポリペプチド転写因子の特異性を再設計する必要がない。CRISPR/Cas技術を、植物種にわたって広く適用可能なウイルス媒介ゲノム編集プラットフォームを開示している国際出願第WO2015/189693号の方法において、植物において実施した。タバコラットルウイルス(TRV)のRNA2ゲノムを、Cas9エンドヌクレアーゼを過剰発現するNicotiana benthamiana植物にガイドRNAを保有および送達するように操作した。本開示の文脈では、ガイドRNAは本明細書に開示される配列のいずれかに由来し得、WO2015/189693の教示を適用して植物細胞のゲノムを編集し、所望の突然変異型植物を得ることができる。ペースの速い技術の開発は、植物界において広範な適用性を有する多種多様なプロトコルを生み出しており、これは多くの最近の科学評論記事において十分に分類されている(例えば、Plant Methods(2016)12:8、およびFront Plant Sci.(2016)7:506)。その用途に特に焦点を当てたCRISPR/Casシステムの評論については、Biotechnology Advances(2015)33,1,41−52)に記載されている。植物ゲノムを操作するためのCRISPR/Casの使用におけるより最近の開発については、Acta Pharmaceutica Sinica B(2017)7,3,292−302、およびCurr.Op.in Plant Biol.(2017)36,1−8に考察されている。植物で使用するためのCRISPR/Cas9プラスミドは、「addgene」、非営利プラスミドリポジトリ(addgene.org)に列挙されており、CRISPR/Casプラスミドは市販されている。
アンチセンス技術は、ポリペプチドの発現を調節するために使用できる別の周知の方法である。抑制される遺伝子のポリヌクレオチドは、RNAのアンチセンス鎖が転写されるように、クローン化され、制御領域および転写終結配列と動作可能に連結される。次いで、組み換え構築物は、植物細胞に形質転換され、RNAのアンチセンス鎖が生成される。ポリヌクレオチドは抑制される遺伝子の全配列である必要はないが、典型的には、抑制される遺伝子のセンス鎖の少なくとも一部分に実質的に相補的であろう。
あるいは、遺伝子は、トランスポゾン(例えば、IS要素)を関心の植物のゲノムに導入することにより、不活性化に標的化することができる。これらの可動性遺伝要素は、有性交雑受精によって導入することができ、挿入変異体をポリペプチド機能の損失についてスクリーニングすることができる。親株における分裂された遺伝子は、親株をトランスポゾン誘発型突然変異誘発の対象ではない植物と、例えば、有性交雑受精によって交雑させることによって、他の植物に導入することができる。当業者にとって既知の任意の標準的な育種技法を利用することができる。一実施形態では、1つ以上の遺伝子は、1つ以上のトランスポゾンの挿入により不活性化できる。突然変異は、1つ以上の遺伝子のホモ接合の分裂、1つ以上の遺伝子のヘテロ接合の分裂、または複数の遺伝子が分裂した場合にはホモ接合およびヘテロ接合の分裂の両方の組み合わせをもたらし得る。好適な転移性要素は、レトロトランスポゾン、レトロポゾン、およびSINE様要素を含む。このような方法は、当業者にとって既知である。
あるいは、遺伝子は、自己切断および複製の能力がある数多くの小型環状RNAに由来するリボザイムを植物に導入することにより、不活性化のために標的化することができる。これらのRNAは、単独(ウイロイドRNA)で、またはヘルパーウイルス(サテライトRNA)と共に、のいずれかで複製することができる。好適なRNAの例には、アボカドサンブロッチウイロイドに由来するもの、ならびにタバコ輪点ウイルス、ルーサン一過性条斑ウイルス、ベルベットタバコ斑紋ウイルス、テリミノイヌホオズキ斑紋ウイルス、および地下性クローバ斑点ウイルスに由来するサテライトRNAが含まれる。様々な標的RNA特異的リボザイムが、当業者に知られている。
個々の植物、植物細胞、または植物材料から調製されたポリヌクレオチドは、突然変異誘発された植物組織、細胞、または材料を起源とする植物の集団における突然変異についてのスクリーニングを促進するために任意にプールすることができる。植物、植物細胞、または植物材料の、後続する1世代以上の世代をスクリーニングすることができる。任意にプールされる群のサイズは、使用するスクリーニング方法の感受性に依存する。
遺伝的改変における使用に好適な植物としては、単子葉および双子葉植物、ならびに植物細胞系が挙げられ、以下の科:Acanthaceae、Alliaceae、Alstroemeriaceae、Amaryllidaceae、Apocynaceae、Arecaceae、Asteraceae、Berberidaceae、Bixaceae、Brassicaceae、Bromeliaceae、Cannabaceae、Caryophyllaceae、Cephalotaxaceae、Chenopodiaceae、Colchicaceae、Cucurbitaceae、Dioscoreaceae、Ephedraceae、Erythroxylaceae、Euphorbiaceae、Fabaceae、Lamiaceae、Linaceae、Lycopodiaceae、Malvaceae、Melanthiaceae、Musaceae、Myrtaceae、Nyssaceae、Papaveraceae、Pinaceae、Plantaginaceae、Poaceae、Rosaceae、Rubiaceae、Salicaceae、Sapindaceae、Solanaceae、Taxaceae、Theaceae、またはVitaceaeのうちの1つに属する種が挙げられる。
突然変異型、非天然型、またはトランスジェニック植物は、例えば、農業において、他の用途を有し得る。例えば、本明細書に説明される突然変異型、非天然型、またはトランスジェニック植物を使用して、動物用の飼料および人間用の食品を製造することができる。
本発明は、本発明をさらに詳細に説明するために提供される、以下の実施例においてさらに説明される。これらの実施例は、本発明を実施するために現時点で企図されている好ましい様式について記載しており、本発明を例証することを意図し、限定することを意図していない。
バーレー種、バージニア種、およびオリエント種タバコ葉の早期乾燥処理時間中の遊離アミノ酸変化に寄与する主要機能を特定するために、収穫時の成熟葉と比較した、48時間の乾燥処理後の乾燥処理された葉における上方制御された遺伝子の関数についての過剰出現分析(log2倍変化>2、調整p値<0.05)を、バーレー種、バージニア種、およびオリエント種タバコにおいて実施する。乾燥処理の種類およびタバコの品種とは無関係に、48時間の乾燥処理後に活性である遊離アミノ酸の産生に関与する遺伝子を特定する。早期乾燥処理中にアスパラギン酸の産生に影響を与え、かつAATファミリーに属するタバコ遺伝子を調査する。
配列番号1:NtAAT2−Sのヌクレオチド配列
atgaacatgtcacaacaatcaccgtcaccgtccgctgaccggaggttgagtgttctggcgagacaccttgaactgtcgtcctccgccaccgtcgaatcctctatcgtcgctgctcctacctctggaaatgctggaaccaactctgtcttctctcacatcgttcgcgctcccgaagatcctattctcggcgtaactctctctctctctctctctctctctcttcatccacacacacacgcactcactcacataacatattaagtatatgcgtgctcaaatgttctgtatgtattcatttgttccgtatcaaatgttctcttgttataagctgaattttagaggaattgtagtgctatttgctaatcgaaagagcttgatactcattctcttcctattgaattaaatattccttttttcttatggatgatgaatttaagacttttttttagtccgatcactacgaaatttcgatttcaagttgatagaagtgaaaaatgatggggttaacatatcaattgagcgaataaaaagagaaattcgtgtgttgatatcttcaaaagtgtatttaaatgtagagatatattgtgatttagtttctgttattatctttgtcttttttctattgaaatttgaatattatttgttgaagtcttcgtgacatatcttggtgttatgttttggttattaggtcactattgcttacaataaagatagtagccccatgaagttgaatttgggagttggtgcatatcgcacagaggtgatcatcctttttggattttgtatttgcgctattatggtcaatggagcactattatcagttgctggataatcatcctttttgatatttccttgattgaaatctaaaaacacgaataaaaagatatttactgatggatctgtgttttggtttcttcagattgacgcatttctgttaattgaaaagaattgtgattgttttggtgattgtggtgttattttagcttcatacagttaatccgacgccgtagtgtactagtgtttggctgatgtgctgccaagagataatgtttaagattatggtttgccataattgataaaatttaatattaaaagtacttggctggatgttctgcgtttgcataacttgtaatgcatatgaaaaagttacctttgattttcataattagtgagaaaactcaagtagcttccgcattcctgtcattgcactatcaaacacattaaacggtttccgacatatctacctagtttggaacttcatgatttctatttttcacaccttgtaataaatgataattcttggatctgtggtgtctttgttcaaaagatcacagagaagattgcatttattttttgtagtctagttggctcagagtctgtcaaacacaacttgttacatcgcattttacctgttagttaagaaacttgggtcatcaacaaatttgtcatgaggtggttatttcttggggctttgtgaattgctctcagcaatctgctagctttcttatgtggactcaaaacaatgaagctcttgagttgatgtgttgatttttcaatcagagtaaaacaagttctatatttggctgtgagagtaaagtgggagctattaaaattcctagctgaatttatgtttcttaatatcttaaatccttaaaggtagagggagaggaaggaggtttattgatgaagggctagtagttgtgtatacttagttctttttcaaatttcataagtatctcttgatggtttttctcgctgactgttgaatatggggctccacagtttgtgttgctatattgaaatgtttcagctaataaaactaacgtgtttctttttctttctcccttttttggggttatcaggaaggaaaacctcttgttttgaatgttgtaagacaagcagagcagctactagtaaatgacaggtacttgcattgccatttcatggagtatgaataaaatgtttccttaattctatgtgattaaacttcaagatttctgcaggtctcgcgttaaagagtacctatctattacgggactggcagacttcaataaattgagtgctaagctgatacttggcgccgacaggtataaaagttcctgttctctgtatagtgttgccgataagatatgcagggagataaagcatgtattttcctgttgcataggatgatatcttcagataataaggctccattccaagtgtttgatggcttggtagatctttgtgaagcatctattaacatttggtcacatttttttaaaaccaacttcccatcccatccatgccattccacgtgtcagttattcatgaaaatgctgttcacttgcatacatgttactgccgttgtgttgatttcctcaactctactcataatttctctgtgtggtcgcattctggtgatctgatttatctgataatatctgtacatgttttgaaatttgggtagtgtctctttgattagcgtgtaaagcaagcaactcttgatgcgtgtgatcaagtgtattgctgtctagagctgacagatgttaaatttatcttatgcgtttccaagatcttccagatgttctatgtaatctttttaggccagcttaaactttgacttgcttcatatacatttatgttaaaggagagttgttaatatacttcaatttttcacatttttaattcctctttttacctgtggtcctcacgagctcttactttctttgcttggtacagccccgctattcaagagaacagagtaacaactgtgcagtgcttgtctggcacaggctcattgagggttggagctgaatttttggctcgacattatcatcaagtaaactgctacatcttcctaacctacctttcattttccttcgttttcttagccttcgtgggtaaacaatcttcaaagttgaattaaccttgatgtaaccattcctgcagcgcacaatttatattccccaaccaacatggggaaaccacccaaaagttttcactttagctggattatcggtaaagagttaccgctactatgatccagcaactcgtggactcaattttcaaggtatgaaacacttccctacaatataatgatgtaacaggatattgtcccattagatatctatggctatgctgtttactattactctcttccaggatgatggatgttcttttagtcttattctggtatttgattacaaattatcacaagtctgaatcaagttgtggatggatggtttcacttgtttgattgcattgtaatccagcaaacttgtaaagtcatcgtcatctatgctttttctttatacctttttctgcgaggaaataagcgaagagagatggagatataacttgataataatggaatgcaacaaacgcctaatttaacatattagggaccaactaacgtcta
配列番号2:配列番号1に記載のNtAAT2−Sの推定ポリペプチド配列
MNMSQQSPSPSADRRLSVLARHLELSSSATVESSIVAAPTSGNAGTNSVFSHIVRAPEDPILGVTIAYNKDSSPMKLNLGVGAYRTEEGKPLVLNVVRQAEQLLVNDRSRVKEYLSITGLADFNKLSAKLILGADSPAIQENRVTTVQCLSGTGSLRVGAEFLARHYHQRTIYIPQPTWGNHPKVFTLAGLSVKSYRYYDPATRGLNFQGLLEDLGSAPSGAVVLLHACAHNPTGVDPTIDQWEQIRRLMRSRGLLPFFDSAYQGFASGSLDTDAQSVRMFVADGGEVLVAQSYAKNMGLYGERVGALSIVCRNADVASRVESQLKLVIRPMYSNPPIHGASIVATILKDRNMYREWTLELKAMADRIIRMRQQLFDALRARGTPGDWSHIIKQIGMFTFTGLNSEQVAFMTKEYHIYMTSDGRISMAGLSSRTVPHLADAIHAAVARAR
配列番号3:NtAAT2−Tのヌクレオチド配列
atgaacatgtcacaacaatcaccgtccgctgaccggaggttgagtgttttggcgaggcaccttgaaccgtcgtcctccgccaccgtcgaaacctccatcgtcgctgctcctacctctggaaatgctggaaccaactctgtcttctctcacatcgttcgtgctcccgaagatcctattctcggggtaactttctctctctctctctctctctctcttcatccacacgcactcactcacataacatatgtataagtatttaagtatatgcgtgctcaaatgttctgtatatattcatttgttccgtatcaaatgttctcttgttataagctgaattttagaggaattgtagtgttatttgctaatcgcaagagcttgcatactcattctcttcgtattgaattaaatattccttttttcttatggatgacgaatttaagcagttttttgagtccgatcactacgaaatttcgatttcaagttgatagaagtgaaaaatgatggtgtttacatattaattgagcgaataaaaagagaaattcgagtgttgatatcttcaaaaatgttgttaaatgtagagatatactgtgatttagtttctgttataatctttgccttttttcttttgaaatttgaatattgtttgttgaagtcttcgtgacatattggtgttatgttttggttattaggttactattgcatacaataaagatagcagccccatgaagttgaatttgggagttggtgcatatcgcacagaggtgatcatcctttttggcttttgtatttgcgctattatcgtcgatggagcactattatcagtagctggataatcatcctttttgatatttccttgattgaaatccaaaaacacgaataaaaagaaatttactgatggatctgtgttttggtttcttcagatttacgcatttctgttaattgaaaaaatattatgattgtcttggtgattgtggtgttgttttggcttcatatagttaatccgacgccgtagtgtactaatgtttggctgatgtgctgccaagagaaatgtttaagattatggtctgccataactgataaaatttaatattaaaagtacttggctggatgttctgcgtttgcataacttgtaacgcatatgaaaaaattacctttgattttcataattagtgagaaaattaagtagcttccgcattcctgtcattgcactatcaaacacatacggtttatgatatatctacctagtttggaactttgtgatttctatttttcacaccttgtaataaatgataattcttggatctgtggtgtctttgttcaaaagatcacagagaagattgcacttatgttttgtagtctagttggctcagactctgtcaaacacaacttgttacatcgcattttacctgttagttaagaaacttgggtcatcaacaaatttgtcatgaggtggttatttcttggggttttgtgaattgctctcagcaatctgctagctttcttatgtggactcaaaacaatgaatctcttgagttgatgtgttgatttttcaatcgagtaaaacaagttctatatttggctgtgagagtaaagtgggagctattaaaattcctagctgaatttatgtttcttaatatcttaaatccttaaaggtagagggagaggaaggaggcttattgatgaaggactagtagttgtgtatacttagttctttctcaaatttcataagaatctcttgagggtttttctcgctgactgttgaatatggggctccacagtttgtgttgctatattgaaatgtttcagctaataatactaacgtgtttctttttctttctcccttttgtggggttatcaggaaggaaaaccgcttgttttgaatgttgtaagacaagcagagcagctactagtaaatgacaggtacttgtgttgccatttcatggagtatgaataaaatgtttccttaattctatgtgattaaacttcaagatttctgcaggtctcgcgttaaagagtacctatctattactggactggcagacttcaataaattgagtgctaagctgatacttggtgccgacaggtatacaagttcctgttctctgtatagtgttgctgataagatatgcagggagataaagcatgtattttcctgttgcataggatgatatcttccgataataaggctccgttccaagtgtttgatggcttggtagatctttgtgaagcatctattaacatttgctcacgtttttttaaaaccaacttcccatcccatccatgccattccacgtgtcagttattcatgaaaatgctgttcacttgcatacatgttactgccgtagtgttggtttctctcaactctactcataatttctgtgtggtcgcattctggtgatctga
配列番号4:配列番号3に記載のNtAAT2−Tの推定ポリペプチド配列
MNMSQQSPSADRRLSVLARHLEPSSSATVETSIVAAPTSGNAGTNSVFSHIVRAPEDPILGVTIAYNKDSSPMKLNLGVGAYRTEEGKPLVLNVVRQAEQLLVNDRSRVKEYLSITGLADFNKLSAKLILGADSPAIQENRVTTVQCLSGTGSLRVGAEFLARHYHQRTIYIPQPTWGNHPKVFTLAGLSVKSYRYYDPATRGLNFQGLLEDLGSAPSGAIVLLHACAHNPTGVDPTIDQWEQIRRLMRSRGLLPFFDSAYQGFASGSLDTDAQSVRMFVADGGEVLVAQSYAKNMGLYGERVGALSIVCRNADVASRVESQLKLVIRPMYSNPPIHGASIVATILKDRNMYHEWTLELKAMADRIIRMRQQLFDALRARGTPGDWSHIIKQIGMFTFTGLNSEQVAFMTKEYHIYMTSDGRISMAGLSSRTVPHLADAIHAAVARAR
配列番号5:NtAAT1−Sのヌクレオチド配列
atggcgatccgagccgcgatttccggtcgtcccctcaagtttagctcgtcggtcggagcgcgatctttgtcgtcgttgtggcgaaacgtcgagccggctcctaaagatcctatcctcggcgttaccgaagctttcctcgccgatcctactcctcataaagtcaatgttggtgttgtaagtttttttttctctttgctttgtttgattttccacttcatttcgtgtaagctaggatttagcttacttgaccatttcgctattcttcataggccatagctgtaaaaatggttttactgtgacgaatcttcgacgatctcaatcgctttgggattgggagagagtttattgatttaatttttgtatgcattccacttttttcaacttgatctatttaagaaaaaaattgaaaaagatttgaccttttttcttaaattatttcttttaaattttttatttttgtgattattatatagggagcttacagagacaacaatggaaaacccgtggtactggagtgtgttagagaagcagagcggaggatcgctggcagtttcaacatgtgagtgctctcctgatttattcaagtttttctgttattttatttgtaattaattacgattacgttaactttgatctattagaaaatagaaacttcagccagtaagattactttttttcttcgaggagtgtgagatgtaaacccaggtcggatgcactggaattcttcactagtttgtgcaaatatattcttaatatttttcaaaaacttcctgtgtacacacacacatacatgtaattaattaagtaattacctactgatctaggatttttaggtagttcggaaaaatatattttcaatatcgtttaagaattttctgggtatgcgtagtttgagtatgataaaatgggtgcatgtgcaccactgcttacgctagggaacagcctctaattagctgttggtgagtctggtgagtggtgactgttctttattttcagttacttcgcacattgttggtttttgattaagtataaataaacgaatgttttagtgagtgcttatttctatgaagcatcttttttaggtctacagaaatgggtggtacgatattttccagccgtcagctccactaaccagtttgattctttgggactttttctttgtattctcacgtttacttctagtggatggtgcagatggatttctttactaattctttcttctgcgtttgcagagctttctcccaagataaattattaatatcaaattgacctttcgatagttcaatggtgtttaactttttcaaatattgcccattttatattatgaagtttgaaagtttaactagacatgttgtaataaattttatttgacttgtgtattttattcattgtggttggagaaagtattaaaataacaagtgaaaagtctgttttacgtcaagtttgaatttgaatttttgaattgatttgcttctttaagtttatgaaaccatctatgagaatattattggcactccattgtctcattttatgtgaaggcatttgacgttgcacaaaatttaaaaatataaagaaacttatgacgtgtaagttagatatatgcagagtaccatagttatccttttaagtaagtgatagtgagagtttcacatttctttttgttctctcttcttataaaagaatgaatttttgtatcccaacaagtcatatcattaatgttaacacggggatactaagattaactaatgtctaaaaagagaaagatttcattcttcttggacgggctaaaaaggaaagtatgtcacataaaatgagacagagatatgttaggatttgtcctgaatttctaatcaattaggactctctttcttgaaggaatggaaaaagactcctaaaaggattaaatctccttaatatgtcttatccttttcttggaagacaagttttgcacatctataaattaaggatctctgcttttcacaagaacacaaaaaaaaaatatccacaatgtagttattaaagagtttgtttagggggagatttttctctcgatagtttttcttcttttatattagttttatcatatgtagatcaattgaccaaatcattataaactattatgcttagtttaatatatttttcttttgttgtctgatttatcgtccatcaagttttgtattgttagttttcgcatgcaccatgttatttcgaacccaacaagtggtatcagagccaatggttcagagtcaacggttgaacgaggttgaagaaagattcaatgcgtgtttagatcaagacgataatgaagatttattcaacatgctacatgtggagataaatttacaattacaattgtattcaacacgcctgctgttgcatctttattggaataaaaactctttctaacccatattttggccactttaaaccaatgccaattttaagtcatgcctacttgcaacacatgagttccagtgccagttttaactcacgcttctttttaatgcatgagcttcttttatcccatgtttattcttaacacgcgggctccttttaacccatacttggtttttttttaaccaataccaagattttcaatt
配列番号6:配列番号5に記載のNtAAT1−Sの推定ポリペプチド配列
MAIRAAISGRPLKFSSSVGARSLSSLWRNVEPAPKDPILGVTEAFLADPTPHKVNVGVGAYRDNNGKPVVLECVREAERRIAGSFNMEYLPMGGSVNMIEESLKLAYGENSDLIKDKRIAAIQALSGTGACRIFADFQRRFCPDSQIYIPVPTWSNHHNIWRDAHVPQKMYHYYHPETKGLDFAALMDDIKNAPNGSFFLLHACAHNPTGVDPTEEQWREISHQFKVKGHFAFFDMAYQGFASGNPEKDAKAIRIFLEDGHPIGCAQSYAKNMGLYGQRVGCLSVVCEDEKQAVAVKSQLQQLARPMYSNPPVHGALVVSTILGDPNLKKLWLGEVKGMADRIIGMRTALRENLEKLGSPLSWEHITNQIGMFCYSGMTPEQVDRLTKEYHIYMTRNGRISMAGVTTGNVGYLANAIHEATKSA
配列番号7:NtAAT1−Tのヌクレオチド配列
atggcgattcgagccgcgatttccggtcgttccctcaagcatattagctcgtcggtcggagcgcgatctttgtcgtcgttgtggcgaaacgtcgagccggctcctaaagatcctatccttggcgttaccgaagctttcctcgccgatcctactccccataaagtcaatgttggcgttgtgagtttttttttcctctttgttttgcttcattttccacctcatttcgtgtatgcaaggatttagcttacttgaccatttcgctatacttcccttggtaggccatagctgtaaaaaatagttttactgtgacgaatcatcgacatatggatacagagtattctaatggagtagtcaacaacataagtcgatctcaatcgctttgggattgagaaagagtttattgatttaatttttgtatgcgttccacttttttcaacttgatctatttaagaaaaaaattgaaaaagatttgaccttttttcttaaattatttcttttataaaatttgcttttgtgattattatacagggagcttacagggacgacaacggaaaacccgtggtactggagtgtgtcagagaagcagagcggaggatcgctggcagtttcaacatgtgagtgcttctcctgatttattcatttttttctgttatttatttgtaattaattacgattacgttaaatttgatctattagaaaatataaacttcagccagtaagattactttttttcttcgaggagtttgagatgtaaaacccaggtcggatgcactgggattcttaagtagtttgtacaaatatattcttaatagttttgtaaaatttgctgtatacacacacatgtaattaattacctactgatctaggatttataggtagttcggaaaaatatattttcaatatcgtttaagaatttcctgggtgtgtatagtttgagtatgagaaaatgggtgcatgtgcaccactgcttacgctagggatcagcttctaaatagctggtggtgagtctggtgagtggtgactgttctttattttcagttactgtagccacaaattgttggttattgattaagtataaataaacgaatgtattagtgagtgcttatttgtatgaagcatcttttttaagtctacagaaatgggtggtccgatattttccacccgtcagttcctctaactagtttgattctttgggactttttctttgtattctcacgtttacgcctagtggatggtgcagatggatttctttactaattctttcttctgcgcttgcagagctttctcccaagataaattattaatatcaaattgacctttcgatagttcaatggtgtttaactttttcaaatattgccccacatcccattttatattatgaagtttgaaaagtttaactagacatgttgtaataaatttttatttgagttgtgtattttattcattgtggtaggagaaactagaaagtattaaaataacaagtgaaaagtctgttttatggataaagaatattacgtcaagtttgaatttgaaattttgaattgatttgcttctttaaatttatgaaaccatctatgagaatattattagcactccatttgtctcattttatgtgaaggcatctgactttgcacaaagttaaaaaatataaagatacttacgacgtgaaagtttgatatatgccaagtaccataattatccttttaagcaagtgatagtgagagtttaacatttctttttgttctctcttcttataaaagaatgaattttgtatcaagtgggtcccaacaagtcattcattaagggtaaaacggggatgctaagattaactaatttccaaaaagagaaagatttaattcttctaggacaggctaaaaatggaaagtgtttcacataaaatgagacatagacaatataagtttgtcaaacattttgtgacgtcctaaaatagaaagtgtcctgagatggaggagaactacgttatctgttctcaaaaaagtgtgtatcatgtgatactatgtggatagtttagttcacatgttaacaattcatcatttatcagggaatatcttcctatgggaggtagtgtcaacatgatcgaggagtcactgaagttagcctatggggagaactcagacttgataaaagataagcgcattgcagcaattcaagctttatctgggactggagcatgccgaatttttgcagacttccaaaggcgcttttgtcctgattcacagatttatattcctgttcctacatggtctaagtaagtgtattcttctgcttctcggcatctctacagcatcctaattgatcttcctcaattggtttttgcacattaaaacatgagtatgcaaataccttcaaaattttctaatttcctgtcattactaatataaaattcttggcagtcatcataacatttggagagatgctcatgtccctcagaaaacgtatcattattatcatcctgaaacaaaggggttggacttcactgcactgatggatgatataaaggtaagaaaacatatatttgaggttgttttccatgatggtttgttctcctgtttgatgatatagcgtccctcctcaagtggcaattatgtgttctatcctgacgtatttcaattttcattgacatagaatgccccaaatggatcattctttctgcttcatgcttgtgctcacaatcctactggggtggatcctacagaggaacaatggagggagatctcgcaccacttcaaggtaatgattttgtatattttgtctctcctttttcttgtaccaagtcatactaaatttattacactggttccaggtgaagggacattttgctttctttgacatggcctatcaaggatttgctagtgggaatccagagaaggatgctaaggcaatcaggatatttcttgaagatggtcatccgataggatgtgcccaatcatatgcaaaaaatatgggactatatggccagagagttggttgcctaaggtaaactactactcccaccatcatatcttatttgccctagttacaatctggagagtcaaactaactttttgttagaccttagtcggtctatttttcaaatgttctaattccaaaagcagttactactatttcctgtaaattctagattaattaactttttattatacctcattatcttcatttagtagctaattttaccatatatcatatttttcatattatcaatatgtagagagaattatttatttaaatattttaagtttatttataaaaaattgagttctttccgataacttcagttcatttccacctcaaagtccaactcgacgtgaaaagcagaattttgctagtcaaaacttggatccaacaattatttagaataaattgagttctttccgataacttcagttcatttccacctcaaagtccaactcgacgagaaaaacagaattttgctagtcaaaacttggatccaatgaaaagcaccaaaattttggttttaaattacaaaataatgtatactctaggtttttgtcctatgcaagtgattttacggtcttaaaataaagcatcaatcaatcccttaaacacacacaaagccctctaatacatttgctgagatgcttccgcaattcactgcagtgtggtttgtgaggatgaaaagcaagcagtggcagtgaaaagtcagttgcagcaacttgctaggcccatgtacagtaatccacctgttcatggtgcgctcgttgtttctaccatccttggagatccaaacttgaaaaagctatggcttggggaagtgaaggtaatgtgattagaacgagataaagatttatgattgtataactatcattggtattttgacgacagataggaactaggaaccttgctattaaagatattttcttgccttaattttgaaaaaagggaattttctcgcttttttggaatgtatgagatttggatagaactatcacatggaaatgctgtaccattttctgctactcacatggaaaagatccttttcttttgctgatctgtttaagcaccaatttgccatagctttgttgtcctatattttcagccacagaaataagtctaaaccagtcccaagctttaataagctttcattgcgtggtagtgtcagccgcataaattggtcagtgcagccattaactgttttcttcatggggcctgttaaccttgtatttctccaaggtcaagttgttggtgttgtggctgctttttagctttgtactcattatcagagcatcatatgttaaacgtaaaggttcaatgactaagagttttttttccagggcatggctgatcgcatcatcgggatgagaactgctttaagagaaaaccttgagaagaagggctcacctctatcgtgggagcacataaccaatcaggtattgaaatcaatgacttctgttgcgttctatactagtatataactattagaacactatggctcacctattgccccattaatactcgatactgcctacagattggcatgttctgctatagtgggatgacacccgaacaagttgaccgtttgacaaaagagtatcacatctacatgactcgtaatggtcgtatcaggtataatcactcattcacgaatttctgcttaatgctccggtgttcttgtacgagttaatatctcattaatttttccactatgttatactgtgtgttttgtatgttgtgcagtatggcaggagttactactggaaatgttggttacttggcaaacgctattcatgaggttaccaaatcagcttaa
配列番号8:配列番号7に記載のNtAAT1−Tの推定ポリペプチド配列
MAIRAAISGRSLKHISSSVGARSLSSLWRNVEPAPKDPILGVTEAFLADPTPHKVNVGVGAYRDDNGKPVVLECVREAERRIAGSFNMEYLPMGGSVNMIEESLKLAYGENSDLIKDKRIAAIQALSGTGACRIFADFQRRFCPDSQIYIPVPTWSNHHNIWRDAHVPQKTYHYYHPETKGLDFTALMDDIKNAPNGSFFLLHACAHNPTGVDPTEEQWREISHHFKVKGHFAFFDMAYQGFASGNPEKDAKAIRIFLEDGHPIGCAQSYAKNMGLYGQRVGCLSVVCEDEKQAVAVKSQLQQLARPMYSNPPVHGALVVSTILGDPNLKKLWLGEVKGMADRIIGMRTALRENLEKKGSPLSWEHITNQIGMFCYSGMTPEQVDRLTKEYHIYMTRNGRISMAGVTTGNVGYLANAIHEVTKSA
配列番号9:NtAAT3−Sのヌクレオチド配列
atggcaaattcctccaattctgtttttgcgcatgttgttcgtgctcctgaagatcccatcttaggagtacgtccctttccactctttctattttacatttccactgaatatgtttcttctgtggctcctttaataatcttccgtaaatatacta
ttagtggatttgataagctacttctctctccctctctcttttattttcttattttgggttagattaaaatgaacattaattaatgatcagatgatttggttaaagatgatatctaggagatcggcataaataagttgattggaatgatcgctatagggtttcctattgtatgcattggatcatggatgtgtgcgctaattatttaatagtacttctttctttttactgtgatctggcaattccttattttattcctggtgtagttgatgaaaggtgtagatttgattctttaacttgctctattgagaaggtaatttgtgcttctcaagtgtttattaatgttgttttcttctgttgtgttacttcattaaaacaggtcacagttgcttataacaaagataccagcccagtgaagttgaatttgggtgttggcgcatatcgcactgaggtctgccacttctactttgtctcgttgttctttattattattatttttttattatagccaaaaaaagttgccccttgaatggatttggtcctgctatgtgttgaatccttggttaagtttttctttaataggctccttcacaaggatagaaaattgtagacactgatgcttacacattagtaatattttttcccctgatgcataatgaagtgaaaccacttgtgcttctaaaaaatcatactttggggcaaggtgaagtacacatttttataagtggttgtttttttcttcaatcttgagttgaatgttagtgttaagtaggagccgcaaacgggcgggtcgggtcggatttggttcagatcgaaaatgggtaatgaaaaaacaggtaaattatctgactcgacccatatttaatacggataaaaacaggttaaccggcggataatatgggtaaccatattattcatgtcttcttgcatatgatcaattatgggagaattcttagcctcaaatgggaacccccaatttgaggctttacaaatttaaaagttagacccattggttaaccattttctaaatggataatatggttcttatccatatttgacccatttttaaaaagttcattatccaacccattttttagtggataatatgggtgtttaactgatttcttttaaccattttgacacccctagtgttaagcttgaaaacgactaatgcatagtctgatgacaacttgcaggaaggaaagccccttgttcttaatgtggtgagacgggctgaacaaatgctcgtcaatgacacgtaacttgccaaattagaaactagcttacagattttcttttgagatatgatcacctgataccaagattggaatctaaggctgctatgatgcaggtctcgggtgaaggagtatctctcaattactggactagcggattttaacaaactgagtgcaaagcttatatttggtgctgacaggtttggagattttttggtgcagttgctcttgataaatgcttgagtcaattttttttaaaaaaaatgctcactatccatgtcgctctatttaaacctatcttgccaaaccacttgtataaatgaaaatgagccgtcgatattcttccttccatctagtttgatatttgaattagagattgttgctaaaagggaatgctttatctctacagcgtagagtaactgaatacctgttaaacatgttcctccgtatttcatcttattatgatgccttgcatctgaagaaaattgttctagagttaactttctctcctctttgttgtactgattatctgtgtgtggtgaacgcgatatcaggaaatatgtgtcttctgtcactattactccttgttaagtcatatgtaattgacttgttatgatatcaacagatttacttatgtttagatgtagtttaaatgctttttgtgctgttttgttgcttatacagccctgccattcaagagaacagggtgactactgttcagtgcttgtcgggcacaggttctttgagggttggggctgaatttctggctaagcattatcatgaagttagtattccttgctctctttccctttatatgtctaaatcaaatggacacttgtataagcttctactgtttgttttgttgccagcataccatatatataccacagccaacatggggaaaccatccgaaggttttcactttagccgggctttcagtaaaatattaccgttactacgacccagcaacacgaggcctggatttccaaggtactactgtaatcactgttcttaaagttctacagttgtaagtaagagccgatttctcttttttatggacaagtgaactttctcctggtcgtgtctagaaagatctatattttatgtgtagctagcacaggatctttatttatttaattttgtattctcttggtaaagatataagcatagtttatctgtggcttttcctgtatttgggtgttgcatatcaaatttaatcatgaaccctgtaggacttttggatgatcttgctgctgcacccgctggagcaatagttcttctccatgcatgtgctcataacccaactggcgttgatccaacaaatgaccagtgggagaaaatcaggcagttgatgaggtccaagggcctgttgcctttctttgacagtgcttaccaggtaaagcttatgatgggattttgaattcaagtgatacttcgttaagaatgattaccaaataatttgaagcgccaaactatgtattaatgggctgcccaacggacccttactataatgaatatttttgatattgcagggttttgccagtggcaacctagatgcagatgcacaatctgttcgcatgtttgtggctgatggtggtgaatgtcttgcagctcagagttatgccaaaaacatgggactgtatggggagcgtgttggtgcccttagcattgtaagtccttttgtcggttgtaattgctttccctttttagtaagcgataaaattggtggctgaagaactatccatggctatatcatgctatctatgtctaaagatgattttccttgaaagcataattcaggttatattccctagaaggctaaaaagaagttgttctgatggtacaatgaacacagtctctagagatattgaaagccaaatttttgaatatggcttcccctttgattgtaattggaaaacaaagagaaggacagagtggaattagtaccggattgtatgtttaggaaaaagtgtcattttgtttgagttttatcagacagacactaaaagctgactaacagtacaataaaattttgtgttgtgttataggtttgcaaagacgcagatgttgcaagcagagtcgaaagccagctaaagctggttatcaggccaatgtactctaatccaccaattcatggtgcgtctattgttgctactatactcaaggacaggtttgtgcaactatttacaagattctgttttgctgttagtagatgctataccttctacattttgatgtggtttctcatctaatggtgatagacaaatgtacgatgaatggacaattgagctgaaagcaatggccgacaggattattagcatgcgccaacaactctttgatgccttgcaagctcgaggtatttgatcttcatatttgttctttctggggaagcatactgtattctgtatgatgggtttgactgctactgcaataggagctttttcctgaaaagtaccatggtgaaacaaccacggcaactaaatcttttgacttcattgttcagtttagtgctaatgtaagttttattctgttatgcaggtacgacaggtgattggagtcatatcatcaagcaaattggaatgtttactttcacaggattaaatactgagcaagtttcattcatgactagagagcatcacatttacatgacatctgatgggtaaggacatctgactattgatattttttttatttgtttagtttgttactttgggttgcttttttctcagtagaaacttaaataattggaacttagaagtccttcgttgattatttcggcttgaattctttaataaggagaatttcagatttatagcttcagtttggagaggaagcataaacaagtctgtcatccatacttaaaatttacagaaaaaagtgcagttctgttttcccccctcccagattagactaattcccaaaagaacttaccttcaatctatggaacatttagtattctggtatcagttgaaacatctctttgttgaagttaagattttggttaaaaagatcttcatctctagtaacattttctacattccatttttagaaggaatgattttctcctttctcatttgcaggagaattagcatggcaggccttagttctcgcacaattcctcatcttgccgatgccatacatgctgctgttaccaaagcggcctaa
配列番号10:配列番号9に記載のNtAAT3−Sの推定ポリペプチド配列
MANSSNSVFAHVVRAPEDPILGVTVAYNKDTSPVKLNLGVGAYRTEEGKPLVLNVVRRAEQMLVNDTSRVKEYLSITGLADFNKLSAKLIFGADSPAIQENRVTTVQCLSGTGSLRVGAEFLAKHYHEHTIYIPQPTWGNHPKVFTLAGLSVKYYRYYDPATRGLDFQGTTVITVLKVLQLYKHSLSVAFPVFGCCISNLIMNPVGLLDDLAAAPAGAIVLLHACAHNPTGVDPTNDQWEKIRQLMRSKGLLPFFDSAYQGFASGNLDADAQSVRMFVADGGECLAAQSYAKNMGLYGERVGALSIVCKDADVASRVESQLKLVIRPMYSNPPIHGASIVATILKDRQMYDEWTIELKAMADRIISMRQQLFDALQARGTTGDWSHIIKQIGMFTFTGLNTEQVSFMTREHHIYMTSDGRISMAGLSSRTIPHLADAIHAAVTKAA
配列番号11:NtAAT3−Tのヌクレオチド配列
atggcaaattcctccaattctgtttttgcccatgttgttcgtgctcctgaagatcccatcttaggagtacctccctttccactctttctattttacatttccactgaatatgtttcttctgtggctcctttaataatcttccgtaaatatattattagtggatttgataagctacttctctctctctctctctctctctctctctctctctctctctctctctctctcttttattttcttattttgggttagattagaatgaacattaattaatgatcagatgattaggttaaaaatgatatcttggagatcggcataaataagttgattggaatgatcgctatagggttacctattgtatgcattggatcatggatgtgtttactaattatttaatacctctttctttttactgtgatctggcaattccttattttattcctggtgtggttgatggaagggtgtagatttgattctttaacttgctctattgagaagataatttgttcttctcaagtgtttagtaatggtttttttcctgttgtgctacttcattaaaacaggtcacagttgcttataacaaagataccagcccggtgaagttgaatttgggtgttggcgcatatcgcactgaggtctgccacttctactttgtctcgttattctttattttttattttttattataaccaaaataagttgccccttgaatggatttggtcctgctatgttttgttgaatccttggttaagtttttctttaataggctccttcacaaggatacaaaattgtagacactgatgcatacacattaatattttttttccctgatgcataatgaagtgaaaccacttgattttataagtggttgtttttttcttcaatcttgagttggatgttagtgttaagcttgaaaattatgttctactaatgcatagtccgatgacaacttgcaggaaggaaagccccttgttcttaatgtggtgagacgagctgaacaaatgctcgtcaatgacacgtaacttgccaaattagaaactagcttacagattttcttttgagatatgatcacctgatgccatgattggaatctaaggctgatatgatgcaggtctcgggtgaaggagtatctctcaattactggactagcggattttaacaaactgagtgcaaagcttatatttggatctgacaggtttggagaatttttggtgcagttgctcttgataaatgcttgaatcaaaaatataaaaaaatgctcactatccatgtcgctccagttaaacctatcttgccaaaccacttgtataaaagaaaatgagccttcaatattcttccttccatctagtttgatatttgaatgagagattgttgctaaaagggaatgctttatctctacaaagtagagtaactgaatacctgttaaaacatattcctccgtatttcatcttattatgatgccttgcatcagaagaaaattgttctagagttaactttctctcctctttgttgtactgactttctgtgtaaggtgaacgtgatatcaggaaatatgtgtcttctatcactattactccttgttaagtcatatgtaagatatcagcagatttacttatctttagatgtagtttaaatgctttttgtgctgttttgttgctgatacagccctgccattcaagagaacagggtgactactgttcagtgcttgtcgggcacaggttctttgagggttggggctgagtttctggctaagcattatcatgaagttagtattccttgctctctttccctttatatgtctaaatcaaatggacacttctataagcttctactgtttgttttgttgccagcatactatatatataccacagccaacatggggaaaccatccgaaggttttcactttagctgggctttcagtaaaatattatcgttactacgacccagcaacacgaggcctggatttccaaggtactactgtaatcaatgttcttaaagttctacagttgtaagtaagaaccgatttctctttttcatggacaagtgaacttgctcctggtcgtgtctagaaagatctatatattatgtgtagctagcacaggatctttatttatttaattttgtattctgttggtaaagatataagcatagtttatctgtggcttctcctgtatttgggtgttgcgtatcaaatttaatcatgaaccctgtaggacttttggatgatcttgctgctgcacccgctggagtaatagttcttctccatgcatgtgctcataacccaactggcgttgatccaacaaatgaccagtgggagaaaatcaggcagttgatgaggtccaaggggctgttacctttctttgacagtgcttaccaggtaaagcttatgatgggattttgaattcaagtgatacttcgttaagaatgattaccaaataatttgaagccccaaactatgtattaatgggctgctcaatggacccctactataatgaatatttttgatattgcagggttttgccactggcaacctagatgcagatgcacaatctgttcgcatgtttgtggctgatggtggtgaatgtcttgcagctcagagttatgccaaaaacatgggactgtatggggagcgtgttggtgcccttagcattgtaagtccttttgtcggttgtaattgctttccctttttaataagcaataaaattgctttccctttttaataagcaatatagcatgatatccatggctatatcatgctatttatgtctaaagatgattttttctttggaagcataattcaggttatattccctaaaaggctaaaaagaggttgttctgttggtacaatgaacacagtctctagagatattgaaagccaattttttgaagatggcttccacttagattgtaattggaaaagaaagagaaggacaaagtggaattagtaccggattgtatgtttaggaaaaagtgtcgttttttttgagttttatcagacaggtactaaaagctgactaacactacaataaaattttgtgttgtgttataggtttgcaaagatgcagatgttgcaagcagagtcgaaagccagctaaagctggttatcaggccaatgtactctaatccaccaattcatggtgcgtctattgttgctactatactcaaggacaggtttgtacaactatatacaagattctgttttgttgttagtagatgctataccttctacattttgatgtggttgctcatctaatggtgatagacaaatgtacgatgaatggacaattgagctgaaagcaatggccgacaggattattagcatgcgccaacaactctttgatgccttgcaagctcgaggtatctgatcttcatatttgttctttctagggaagcatactgtattctgtatgatgggtttgactgctactgcaataggaactttttctggaaaagtgccagggtgaaagaaccacggcaactaaatcttctgacttcattgttcagtttagtgctaatgtaagttttattctgttatgcaggtacagcaggtgattggagtcatatcatcaaacaaattggcatgtttactttcacaggattgaatactgagcaagtttcattcatgactagagagcatcacatttacatgacatctgatgggtaaggacatctgactgttgatatttttttttatttgtttagtttgttactttgggttgcttttttctcagtagaaacttaaataattggaacttagaagcccttatcattgattatttcggcttgaattctttaataaggagaatttcagacttatagcttcagttttgagaggaagcataaacaagtccagctctgtcattcatacttaaaatttacagaagaaagtgcagttctgtttttcccccctcccaaattatattgattctcaaaagaacttaccttcaatctatggcacatttagtaatctggtatcagttgaaacatctctttgttgaagttaagattttggttaaaaagatcatcatctctagtgacattttctactttccatttttagaaggaatgattttctcctttctcatttgcaggagaattagcatggcaggccttagttctcgcacaattcctcatcttgccgatgccatacatgctgctgttaccaaagcggcctaa
配列番号12:配列番号11に記載のNtAAT3−Tの推定ポリペプチド配列
MANSSNSVFAHVVRAPEDPILGVTVAYNKDTSPVKLNLGVGAYRTEEGKPLVLNVVRRAEQMLVNDTSRVKEYLSITGLADFNKLSAKLIFGSDSPAIQENRVTTVQCLSGTGSLRVGAEFLAKHYHEHTIYIPQPTWGNHPKVFTLAGLSVKYYRYYDPATRGLDFQGTTVINVLKVLQLYKHSLSVASPVFGCCVSNLIMNPVGLLDDLAAAPAGVIVLLHACAHNPTGVDPTNDQWEKIRQLMRSKGLLPFFDSAYQGFATGNLDADAQSVRMFVADGGECLAAQSYAKNMGLYGERVGALSIVCKDADVASRVESQLKLVIRPMYSNPPIHGASIVATILKDRQMYDEWTIELKAMADRIISMRQQLFDALQARGTAGDWSHIIKQIGMFTFTGLNTEQVSFMTREHHIYMTSDGRISMAGLSSRTIPHLADAIHAAVTKAA
配列番号13:NtAAT4−Sのヌクレオチド配列
atggtttccacaatgttctctctagcttctgccactccgtcagcttcattttccttgcaagataatctcaaggtaatttcatcgtcaattacattatttggaaatttgccttatcttagactattcctaatgaggtggattcatgctgttgtttgtgtttgaacagtcaaagctaaagctggggactactagccaaagtgcctttttcgggaaagacttcgtgaaggcaaaggtaggatttttgtgttgtttgtgtacatttggtgagaggtaatagctctactgctatagagaaactccctgtaggttctgtcctttagagtatagaagagaaggaaagagtttaattgggaataatggtggggatgggatgatttgcatacaattgaacatgtgtttcttgctttggtatattatgatataggatgatccaatcatgctccgtaaatcaactccagaacttattattctttcggcacttactaattataaaaatcgggttggagtcctgaaaataagtgattgcctaaccaacttacagaactaattttattatccgtatactcaaatcaaaacgacattatgccagtactggtttcttgagagggatgatattagtgtagaattatttataaagttgcagtttaacgtagggtgttttactaaccagaaaggtgtagatgattccattcagtttattagatgctaagaagtataacagtgaggcctgtgaaacttctggtagtaccaacgattggggttttatggcgtttaggaatttagacattaattggcacattttagaacgaaaaatatgacatttaacttacaacagttcttttctgaataaaatattactagtaactaatttgtttgaactttgccattgctaaaatgtggctcaagatcttcttggtacttctatttgtaatatcagagttataggggtctaattctagctcttgagtcgaaattgttattagtaaagataattctttcttgtcccccttcagtgctaacattctcatcttcacttatggtattggtttataaaaaattgtgattcagattataaagtaaaaaattatgcctcagtttgtacagcattttgggttatctgacgttcaattcaacagggttctttaatatctatttttctatcttttgtaatcattgcaacaccgagctgtttaatgtgctcaaaggctattattagtcctcccactcaccagatccttagaaaaaagcccagaagagaaaggcaaagaatacaagcccagaccgattgctcgttttataaattttgggaattgggatctcttttctcatattcttacttttttctctttctttttttccagtcaaatggccgtactactatgactgttgctgtgaacgtctctcgatttgagggaataactatggctcctcctgaccccattcttggagtttctgaagcattcaaggctgatacaaatgaactgaagcttaaccttggtgttggagcttaccgcacggaggagcttcaaccatatgtcctcaatgttgttaagaaagtaagttcttggtctcttgtttatgctcaagtagtttgtaaacttttagtcacttggccttgttcccatgggtggatacccttgtccaaggggagtcaatttattacactctgtaaataggttaattcttttttaaaatgtatgtatgtatgtatgtatgtatgtatgtatacacacacactatgttgaatcgcccctggcttcttctgtttacttctatatattttgtatccaatgggtgaaaattctggagtgactgcttgttcctaagcgttcatcattcattaactgttttaataaccttctataattttgcatctgaatgatgaggaaattgcttttctgtaggcagaaaaccttatgctagaaagaggagataacaaagaggtacttgatttactaaattcatcttttggccttgactagtgtcacttggtgccaattcttacttattttttaatctatggatatatagtatcttccaatagaaggtttggctgcattcaacaaagtcacagcagagttattgtttggagcagataacccagtgattcagcaacaaagggtaagtatttttgtttttaactcttaggaaaatatatcctggaacaaacatgtaaatttggtctctatggcctttgttgtgaacgacgttgtacctttcgtgatcaggtggctactattcaaggtctgtcaggaactgggtcattgcgtattgctgcagcactgatagagcgttacttccctggctctaaggttttaatatcatctccaacctggggtacgtagatagtgcttttggattaatttggttgaatctcatgatactgatttttacagttatgttttgcaggaaatcataagaacattttcaatgatgccagggtgccttggtctgaatatcgatattatgatcccaaaacagttggcctggattttgctgggatgatagaagatattaaggttattatcgtcctcgcatttgtaatctttgtggttgaaattgtaaagcagcagtgagcactgtctttttcctttctccacaagtcaattgatggtgcctttgtttgtggcacgtgttttgactttcagtaattgaaggagagatgcgttgttcattctagaatagcactgtatctcccaattgcattttctgtttcctgttcttcctccctatgtttgcattgatccatgtctctgctaaacatggacaatttgcgcccttggcaatgacatgtgtgttgcttgcttttcttctctttctatttcttggtaggagtgacttggttctttcaatgtgagcagtcatatttctgaaaatgaaaatcagaggaacttgggatgtggaagggattggcagaacaagtttgataatgtaatttttcttgtgaggatggaatatgcaaaaataggctgcacgcttgccttttagatctttggttcctatgtcggttgtgaatgtagatttctatttttcaacattgtctcgcaaggaaaataggattatccagtattggatgtctttcctatgtttgatatgtgtatgtgcagtcttgtttgaccgtcttgctctcttcccacgtctaaaaagagagtctgatgggaaagtttttttccttccagttcttgtgcaagtcattgacatagtttatggcattacttgtttataggctgctcctgaaggatcatttatcttgctccatggctgtgcgcacaacccaactggtattgatcccacaattgaacaatgggaaaagattgctgatgtaattcaggagaagaaccacattccattttttgatgtcgcctaccaggtaatctgtgctaaacccaattatttcatttggtgaagctgtaaaatttcaagtttcttagaagttttgatggttgtgtgtgcgtgtgaagagaatgaatgatataggaattggttttgaaatagtgaaagatctctcgtatttcatttgttcttttggtgtgaggagagtatacattgttgttttgatagatgggcaaattcgatagatgaaggtggttaagccacgtgttactttgtaatttttttttgacaccgtcatggtgtttatcaataaaatttactgatttttcagtaaagttattagaacaagataatctgaagtcatttctattcagagaattgcattgaatagctgtatactataataatcgagatgcctcatctgtctacacgctgccctacagggatttgcaagcggcagccttgacgaagatgcctcatctgtgagattgtttgctgcacgtggcatggagcttttggttgctcaatcatatagtaaaaatctgggtctgtatggagaaaggattggagctattaatgttctttgctcatccgctgatgcagcgacaaggtacagtcacccgcactagcaactacataattgtcctctgtataggaaaaatgatgcactggaaaacaatggttccatatgaaatgccaattacgagatgctgtccctttgctttgatattgtttactacaattggtatctcccatcacctgagcctatggcttgattggattttatgtgggcgaaccaatagaattatttgcttaattttctcaactaatggatgcatctctgctaactcacagggtgaaaagccagctaaaaaggcttgctcgaccaatgtactcaaatcccccaattcacggtgctagaattgttgccaatgtcgttggaattcctgagttctttgatgaatggaaacaagagatggaaatgatggcaggaaggataaagagtgtgagacagaagctatacgatagcctctccaccaaggataagagtggaaaggactggtcatacattttgaagcagattggaatgttctccttcacaggcctcaacaaagctcaggtaaatccccgtgatttaagctattgcttcatcacaatatgcttaaattcaatttgatcattcatcgcaaagcacattctgaactcagcacatattttcattaacacattctttccgtcctttctgatcaattccataagtccgatatgcaaaagatagtgcagtgagagtctcttactggagtataactagattatcgacaatgcatacatttctttccctgtacctgcacttctggtgctcatatttgatctctcttcttggccacgcagagcgagaacatgaccaacaagtggcatgtgtacatgacaaaagacgggaggatatcgttggctggattatcagctgctaaatgcgaatatcttgcagatgccataattgactcgtactacaatgtcagctaa
配列番号14:配列番号13に記載のNtAAT4−Sの推定ポリペプチド配列
MVSTMFSLASATPSASFSLQDNLKSKLKLGTTSQSAFFGKDFVKAKSNGRTTMTVAVNVSRFEGITMAPPDPILGVSEAFKADTNELKLNLGVGAYRTEELQPYVLNVVKKAENLMLERGDNKEYLPIEGLAAFNKVTAELLFGADNPVIQQQRVATIQGLSGTGSLRIAAALIERYFPGSKVLISSPTWGNHKNIFNDARVPWSEYRYYDPKTVGLDFAGMIEDIKAAPEGSFILLHGCAHNPTGIDPTIEQWEKIADVIQEKNHIPFFDVAYQGFASGSLDEDASSVRLFAARGMELLVAQSYSKNLGLYGERIGAINVLCSSADAATRVKSQLKRLARPMYSNPPIHGARIVANVVGIPEFFDEWKQEMEMMAGRIKSVRQKLYDSLSTKDKSGKDWSYILKQIGMFSFTGLNKAQSENMTNKWHVYMTKDGRISLAGLSAAKCEYLADAIIDSYYNVS
配列番号15:NtAAT4−Tのヌクレオチド配列
atggcttccacaatgttctctctagcttctgccgctccatcagcttcattttccttgcaagataatctcaaggtaatttcattgtgaattacattatttggaaatttgccctatcttagactgttcctaatgaggtggattcatgctgttgtttgtgtttgaacagtcaaagctaaagctggggactactagccaaagtgcctttttcgggaaagacttcgcgaaggcaaaggtaggatttttgtgttgtttgtgtacatttggtgagaggtaatagctctactgatatagagcaactccctgtaggttctgtcctttagagtatagaagagaagagaagagtttaattgggaataatggtggggatggaatgatttgcatacaaatgaacatgtgtttcttgcttttggtgtatgatataggatgatccaatcatgctccgtaaatcaactccagaacttattattctttcggcacttctaattataaaaatctggttggagtaatgaatataagtgattacctaaccaacttacagaattgattttattatccatatactgaaattcaaaaacggcgttttgccagtactggtttcttgagagggatgatattaatatagaattattttataaagttgcagtttaacgtagggtattttactaactagaaaggtgatagatggttccgttcagtttattagaagtataacagtgaggcctgttaaacttttgctagtatcaatgattggggttttatggcgtttaggaatttagacatcaattggcacattttagaacgaaaaacatgacatttaagttacatcagttcttttctgaataaaatagtactagtaaataacttgtttgaactttgccatttgctaaaatgtggctcaagatcttcttggtacttctatttgtaatatcagagttataggggtctaattctaccactgttttgagtcaaaatgttattagtaaagataattctttcttgtcccccttcagtgctaacattctcatcttcaattatggtattggtttataaaaaaattgtgcttcagatcactttataaagcaaaaattatgcctcagtttgtacagcattttgggttttataacattcaattcaacagggctctttaatatctatgtttctactttttgtaatctacatcgagctgtttaatgtgctcaaaggctttaattagtcctcctactcaccagatccttagaaaaaagcccagaagagaaaggcaaagacaacgagctcggacagattgctcaatttatattgcaaaaagatccaaaccctcggggagggaggagcatgaaccaaagatgatacattgatattattttctaaatttgggaattgtgatcttatcttaaatttttacttttttctctttttctttttttatagtcaaatggtcggactactatggctgtttctgtgaacgtctctcgatttgagggaataacaatggctcctcctgaccccattcttggagtttctgaagcattcaaggctgatacaaatgaactgaagcttaaccttggagttggagcttaccgcacagaagatcttcaaccctatgtcctcaatgttgttaaaaaagtaagtcctcggtctcttgtttatgctcaacgtagtttgtaaactaagagtcacttaaccttgttcccatgtgttcgtcattaaacatagtaataactttctatagttttgcatctgaatgatgaggaaattacttttctgtaggcagaaaaccttatgctagagagaggtgacaacaaagaggtacttgatatactaaattcatcttttggcctattagtgtctcttggtgccatttcttacttattttttgtccatgaatatatagtatcttccaatagaaggtttggctgcattcaacaaagtcacagcagagttattgtttggagcagataatccagtgattcagcaacaaagggtaagtattttggtttttaactcttagcaaaaaagtatcctggaacaaacttgtagattcagtttccacggattgaatggcattgtatgtttcttgatcaggtggctactattcaaggtctatcaggaactgggtcattgcgtattgctgcagcactgatagagcgttacttccctggctctaaggttttgatatcatctccaacctggggtacgtatatagtgctttggattaatttggttgaatctcataatactgatttttgcagttatgttttgcaggaaatcataagaacattttcaatgatgccagggtgccttggtctgaatatcgatattatgatcccaaaacagttggtctagattttgctgggatgata
配列番号16:配列番号15に記載のNtAAT4−Tの推定ポリペプチド配列
MASTMFSLASAAPSASFSLQDNLKSKLKLGTTSQSAFFGKDFAKAKSNGRTTMAVSVNVSRFEGITMAPPDPILGVSEAFKADTNELKLNLGVGAYRTEDLQPYVLNVVKKAENLMLERGDNKEYLPIEGLAAFNKVTAELLFGADNPVIQQQRVATIQGLSGTGSLRIAAALIERYFPGSKVLISSPTWGNHKNIFNDARVPWSEYRYYDPKTVGLDFAGMIEDIKAAPEGSFILLHGCAHNPTGIDPTIEQWEKIADVIQEKNHIPFFDVAYQGFASGSLDEDASSVRLFAARGMELLVAQSYSKNLGLYGERIGAINVLCSSADAATRVKSQLKRLARPMYSNPPIHGARIVANVVGIPEFFDEWKQEMEMMAGRIKSVRQKLYDSLSAKDKSGKDWSYILKQIGMFSFTGLNKAQSENMTNKWHVYMTKDGRISLAGLSAAKCEYLADAIIDSYYNVS
配列番号17:AAT2S/T RNAi植物を生成するために使用されるヌクレオチド配列
gctattcaagagaacagagtaacaactgtgcagtgcttgtctggcacaggctcattgagggttggagctgaatttttggctcgacattatcatcaacgcac
Claims (12)
- 植物細胞であって、
(i)配列番号1、配列番号3、配列番号5、配列番号7、配列番号9、配列番号11、配列番号13、もしくは配列番号15に対して少なくとも80%の配列同一性を有する配列を含むか、それからなるか、またはそれから本質的になるポリヌクレオチド、
(ii)(i)に記載した前記ポリヌクレオチドによりコードされるポリペプチド、
(iii)配列番号6もしくは配列番号8に対して少なくとも95%の配列同一性、配列番号2、もしくは配列番号10、もしくは配列番号12に対して少なくとも93%の配列同一性、または配列番号4、もしくは配列番号14、もしくは配列番号16に対して少なくとも94%の配列同一性を有する配列を含むか、それからなるか、またはそれから本質的になるポリペプチド、あるいは
(iv)(i)に記載した前記単離ポリヌクレオチドを含む構築物、ベクター、または発現ベクターを含み、
前記植物細胞が、前記ポリヌクレオチドまたは前記ポリペプチドの発現または活性が改変されていない対照植物細胞と比較して、前記ポリヌクレオチドまたは前記ポリペプチドの発現または活性を調節する少なくとも1つの改変を含む、植物細胞。 - 前記植物細胞が、配列番号5、配列番号7、配列番号1、もしくは配列番号3に対して少なくとも80%の配列同一性を有する配列を含むか、それからなるか、またはそれから本質的になるポリヌクレオチドを含み、好適には、前記植物細胞が、配列番号1もしくは配列番号3に対して少なくとも80%の配列同一性を有する配列を含むか、それからなるか、またはそれから本質的になるポリヌクレオチドを含むか、あるいは
前記植物細胞が、配列番号6もしくは配列番号8に対して少なくとも95%の配列同一性、または配列番号2に対して少なくとも93%の配列同一性、または配列番号4に対して少なくとも94%の配列同一性を有する配列を含むか、それからなるか、またはそれから本質的になるポリペプチドを含み、好適には、前記植物細胞が、配列番号2に対して少なくとも93%の配列同一性、または配列番号4に対して少なくとも94%の配列同一性を有する配列を含むか、それからなるか、またはそれから本質的になるポリペプチドを含む、請求項1に記載の植物細胞。 - 前記少なくとも1つの改変が、前記植物細胞のゲノムの改変、または前記構築物、ベクター、もしくは発現ベクターの改変、またはトランスジェニック改変であり、好ましくは
前記植物細胞のゲノムの前記改変、または前記構築物、ベクター、もしくは発現ベクターの前記改変が、突然変異または編集である、請求項1または2に記載の植物細胞。 - 前記改変が、前記対照植物細胞と比較して、前記ポリヌクレオチドまたは前記ポリペプチドの発現または活性を減少させ、好ましくは、
前記植物細胞が、請求項1(i)の前記ポリヌクレオチドから転写されたRNAの少なくとも19ヌクレオチドに対して少なくとも80%相補的である配列を含む干渉ポリヌクレオチドを含む、請求項1〜3のいずれか一項に記載の植物細胞。 - 前記ポリヌクレオチドまたは前記ポリペプチドの前記調節された発現または活性が、対照植物に由来する乾燥処理されたまたは乾燥された葉におけるアミノ酸のレベルと比較して、前記植物細胞に由来する乾燥処理されたまたは乾燥された葉におけるアミノ酸のレベルを調節し、好適には、前記アミノ酸がアスパラギン酸またはそれに由来する代謝産物であり、かつ/あるいは
前記植物細胞に由来する乾燥処理されたまたは乾燥された葉におけるニコチンのレベルが、対照植物細胞の乾燥処理されたまたは乾燥された葉におけるニコチンのレベルと実質的に同じであり、かつ/あるいは
前記植物細胞に由来する乾燥処理されたまたは乾燥された葉におけるアクリルアミドのレベルが、対照植物に由来する乾燥処理されたまたは乾燥された葉におけるアクリルアミドのレベルと比較して減少し、かつ/あるいは
前記植物細胞に由来する乾燥処理されたまたは乾燥された葉におけるアンモニアのレベルが、対照植物に由来する乾燥処理されたまたは乾燥された葉におけるアミノ酸のレベルと比較して減少する、請求項1〜4のいずれか一項に記載の植物細胞。 - 請求項1〜5のいずれか一項に記載の植物細胞を含む、植物またはその部分であって、好ましくは、
前記アスパラギン酸もしくはそれに由来する代謝産物および/またはアンモニアの量が、対照植物またはその部分と比較して、前記植物の少なくとも一部分において改変されている、植物またはその部分。 - 請求項6に記載の植物またはその部分に由来する植物材料、乾燥処理された植物材料、または均質化された植物材料であって、好ましくは、
前記乾燥処理された植物材料が、空気乾燥処理または日光乾燥処理または熱風送管乾燥処理された植物材料であり、好ましくは、
前記植物材料、乾燥処理された植物材料、または均質化された植物材料が、請求項6に記載の植物またはその部分に由来するバイオマス、種子、茎、花、または葉を含む、植物材料、乾燥処理された植物材料、または均質化された植物材料。 - 請求項1〜5のいずれか一項に記載の植物細胞、請求項6に記載の植物の部分、または請求項7に記載の植物材料を含む、タバコ製品。
- 請求項6に記載の植物を生成するための方法であって、
(a)配列番号1、配列番号3、配列番号5、配列番号7、配列番号9、配列番号11、配列番号13、もしくは配列番号15に対して少なくとも80%の配列同一性を有する配列を含むか、それからなるか、またはそれから本質的になるポリヌクレオチドを含むか、それからなるか、またはそれから本質的になるポリヌクレオチドを含む植物細胞を提供する工程、
(b)対照植物細胞と比較して、前記ポリヌクレオチドの発現を調節するために前記植物細胞を改変する工程、および
(c)前記植物細胞を植物中に伝播させる工程を含む、方法。 - 工程(c)が、前記植物細胞を含む挿し木または苗木から前記植物を栽培することを含み、かつ/あるいは
前記植物細胞を改変する前記工程が、ゲノム編集またはゲノム工学によって前記細胞のゲノムを改変することを含み、好ましくは、
前記ゲノム編集またはゲノム工学が、CRISPR/Cas技術、ジンクフィンガーヌクレアーゼ媒介突然変異誘発、化学的突然変異誘発または放射線突然変異誘発、相同組み換え、オリゴヌクレオチド特異的突然変異誘発、およびメガヌクレアーゼ媒介突然変異誘発から選択される、請求項16に記載の方法。 - 前記植物細胞を改変する前記工程が、配列番号1、配列番号3、配列番号5、配列番号7、配列番号9、配列番号11、配列番号13、もしくは配列番号15に対して少なくとも80%の配列同一性を有する配列を含むか、それからなるか、またはそれから本質的になるポリヌクレオチドを含み、構成的プロモーターに動作可能に連結されている構築物で前記細胞をトランスフェクトすることを含み、かつ/あるいは
前記植物細胞を改変する前記工程が、請求項1(i)のポリヌクレオチドから転写されたRNAに対して少なくとも80%相補的である配列を含む干渉ポリヌクレオチドを前記細胞へ導入することを含み、好ましくは、
前記植物細胞が、請求項1(i)のポリヌクレオチドから転写されたRNAの少なくとも19ヌクレオチドに対して少なくとも80%相補的である配列を含む干渉ポリヌクレオチドを発現する構築物でトランスフェクトされる、請求項9または請求項10に記載の方法。 - 対照植物材料と比較して変更された量のアスパラギン酸もしくはそれに由来する代謝産物、または変更された量のアンモニアを含む、乾燥処理された植物材料を製造する方法であって、
(a)請求項6に記載の植物もしくはその部分、または請求項7に記載の植物材料を提供する工程、
(b)任意に、前記植物材料をそこから収穫する工程、および
(c)前記植物材料を乾燥処理する工程を含み、好ましくは、
前記植物材料が、乾燥処理された葉、乾燥処理された茎、もしくは乾燥処理された花、またはそれらの混合物を含み、かつ/あるいは
前記乾燥処理方法が、空気乾燥処理、火力乾燥処理、燻煙乾燥処理、および熱風送管乾燥処理からなる群から選択される、方法。
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