JP6831776B2 - ２個のリガンドと１個のレセプタとからなる高親和性錯体の特定方法と該方法を実行する装置及び該方法に用いられる自己集合型ケミカルライブラリ - Google Patents
２個のリガンドと１個のレセプタとからなる高親和性錯体の特定方法と該方法を実行する装置及び該方法に用いられる自己集合型ケミカルライブラリ Download PDFInfo
- Publication number
- JP6831776B2 JP6831776B2 JP2017502621A JP2017502621A JP6831776B2 JP 6831776 B2 JP6831776 B2 JP 6831776B2 JP 2017502621 A JP2017502621 A JP 2017502621A JP 2017502621 A JP2017502621 A JP 2017502621A JP 6831776 B2 JP6831776 B2 JP 6831776B2
- Authority
- JP
- Japan
- Prior art keywords
- ligand
- rna
- receptor
- site
- complex
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 239000003446 ligand Substances 0.000 title claims description 234
- 238000000034 method Methods 0.000 title claims description 61
- 239000000126 substance Substances 0.000 title claims description 17
- 108020004414 DNA Proteins 0.000 claims description 129
- 102000053602 DNA Human genes 0.000 claims description 96
- 108091032973 (ribonucleotides)n+m Proteins 0.000 claims description 87
- 108020004682 Single-Stranded DNA Proteins 0.000 claims description 74
- 230000000295 complement effect Effects 0.000 claims description 38
- 238000009396 hybridization Methods 0.000 claims description 30
- 238000000926 separation method Methods 0.000 claims description 14
- 108090000623 proteins and genes Proteins 0.000 claims description 13
- 102000004169 proteins and genes Human genes 0.000 claims description 13
- 102000040650 (ribonucleotides)n+m Human genes 0.000 claims description 9
- 108091028043 Nucleic acid sequence Proteins 0.000 claims description 7
- 108091023037 Aptamer Proteins 0.000 claims description 3
- 238000004458 analytical method Methods 0.000 claims description 3
- 150000001720 carbohydrates Chemical class 0.000 claims description 3
- 235000014633 carbohydrates Nutrition 0.000 claims description 3
- 150000002632 lipids Chemical class 0.000 claims description 3
- 102000004196 processed proteins & peptides Human genes 0.000 claims description 3
- 108090000765 processed proteins & peptides Proteins 0.000 claims description 3
- 239000000758 substrate Substances 0.000 claims description 3
- 238000004566 IR spectroscopy Methods 0.000 claims description 2
- 229920002684 Sepharose Polymers 0.000 claims description 2
- 229920001222 biopolymer Polymers 0.000 claims description 2
- 239000000919 ceramic Substances 0.000 claims description 2
- 238000004587 chromatography analysis Methods 0.000 claims description 2
- 238000004817 gas chromatography Methods 0.000 claims description 2
- 239000011521 glass Substances 0.000 claims description 2
- 238000004128 high performance liquid chromatography Methods 0.000 claims description 2
- 239000000017 hydrogel Substances 0.000 claims description 2
- 238000004949 mass spectrometry Methods 0.000 claims description 2
- 229920001059 synthetic polymer Polymers 0.000 claims description 2
- 230000001771 impaired effect Effects 0.000 claims 1
- 230000001939 inductive effect Effects 0.000 claims 1
- 239000002773 nucleotide Substances 0.000 claims 1
- 125000003729 nucleotide group Chemical group 0.000 claims 1
- 239000000243 solution Substances 0.000 description 20
- 238000002474 experimental method Methods 0.000 description 17
- 238000001816 cooling Methods 0.000 description 12
- 230000035945 sensitivity Effects 0.000 description 10
- 239000012530 fluid Substances 0.000 description 9
- OPTASPLRGRRNAP-UHFFFAOYSA-N cytosine Chemical compound NC=1C=CNC(=O)N=1 OPTASPLRGRRNAP-UHFFFAOYSA-N 0.000 description 8
- UYTPUPDQBNUYGX-UHFFFAOYSA-N guanine Chemical compound O=C1NC(N)=NC2=C1N=CN2 UYTPUPDQBNUYGX-UHFFFAOYSA-N 0.000 description 8
- 238000010438 heat treatment Methods 0.000 description 7
- 102000003960 Ligases Human genes 0.000 description 6
- 108090000364 Ligases Proteins 0.000 description 6
- 230000015572 biosynthetic process Effects 0.000 description 6
- 238000001514 detection method Methods 0.000 description 5
- 229930024421 Adenine Natural products 0.000 description 4
- GFFGJBXGBJISGV-UHFFFAOYSA-N Adenine Chemical compound NC1=NC=NC2=C1N=CN2 GFFGJBXGBJISGV-UHFFFAOYSA-N 0.000 description 4
- ISAKRJDGNUQOIC-UHFFFAOYSA-N Uracil Chemical compound O=C1C=CNC(=O)N1 ISAKRJDGNUQOIC-UHFFFAOYSA-N 0.000 description 4
- 229960000643 adenine Drugs 0.000 description 4
- 229940104302 cytosine Drugs 0.000 description 4
- 230000000694 effects Effects 0.000 description 4
- 230000036963 noncompetitive effect Effects 0.000 description 4
- RWQNBRDOKXIBIV-UHFFFAOYSA-N thymine Chemical compound CC1=CNC(=O)NC1=O RWQNBRDOKXIBIV-UHFFFAOYSA-N 0.000 description 4
- DEQPBRIACBATHE-FXQIFTODSA-N 5-[(3as,4s,6ar)-2-oxo-1,3,3a,4,6,6a-hexahydrothieno[3,4-d]imidazol-4-yl]-2-iminopentanoic acid Chemical compound N1C(=O)N[C@@H]2[C@H](CCCC(=N)C(=O)O)SC[C@@H]21 DEQPBRIACBATHE-FXQIFTODSA-N 0.000 description 3
- 108010090804 Streptavidin Proteins 0.000 description 3
- 238000009825 accumulation Methods 0.000 description 3
- 238000006243 chemical reaction Methods 0.000 description 3
- 238000004925 denaturation Methods 0.000 description 3
- 230000036425 denaturation Effects 0.000 description 3
- 239000007788 liquid Substances 0.000 description 3
- PXFBZOLANLWPMH-UHFFFAOYSA-N 16-Epiaffinine Natural products C1C(C2=CC=CC=C2N2)=C2C(=O)CC2C(=CC)CN(C)C1C2CO PXFBZOLANLWPMH-UHFFFAOYSA-N 0.000 description 2
- 229930010555 Inosine Natural products 0.000 description 2
- UGQMRVRMYYASKQ-KQYNXXCUSA-N Inosine Chemical compound O[C@@H]1[C@H](O)[C@@H](CO)O[C@H]1N1C2=NC=NC(O)=C2N=C1 UGQMRVRMYYASKQ-KQYNXXCUSA-N 0.000 description 2
- 239000011230 binding agent Substances 0.000 description 2
- 238000013461 design Methods 0.000 description 2
- 229960003786 inosine Drugs 0.000 description 2
- 230000003993 interaction Effects 0.000 description 2
- 238000013021 overheating Methods 0.000 description 2
- 229940113082 thymine Drugs 0.000 description 2
- 238000011282 treatment Methods 0.000 description 2
- 229940035893 uracil Drugs 0.000 description 2
- 108091008102 DNA aptamers Proteins 0.000 description 1
- 108091008103 RNA aptamers Proteins 0.000 description 1
- 230000002411 adverse Effects 0.000 description 1
- 239000000872 buffer Substances 0.000 description 1
- 238000012512 characterization method Methods 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 239000012459 cleaning agent Substances 0.000 description 1
- 230000009918 complex formation Effects 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 230000008878 coupling Effects 0.000 description 1
- 238000010168 coupling process Methods 0.000 description 1
- 238000005859 coupling reaction Methods 0.000 description 1
- 239000000539 dimer Substances 0.000 description 1
- 239000007850 fluorescent dye Substances 0.000 description 1
- 239000012634 fragment Substances 0.000 description 1
- 239000003112 inhibitor Substances 0.000 description 1
- 238000002347 injection Methods 0.000 description 1
- 239000007924 injection Substances 0.000 description 1
- 238000002955 isolation Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 238000011002 quantification Methods 0.000 description 1
- 238000005215 recombination Methods 0.000 description 1
- 230000006798 recombination Effects 0.000 description 1
- 238000003757 reverse transcription PCR Methods 0.000 description 1
- 108091069025 single-strand RNA Proteins 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 230000003068 static effect Effects 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6811—Selection methods for production or design of target specific oligonucleotides or binding molecules
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L7/00—Heating or cooling apparatus; Heat insulating devices
- B01L7/52—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples
- B01L7/525—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples with physical movement of samples between temperature zones
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/5308—Immunoassay; Biospecific binding assay; Materials therefor for analytes not provided for elsewhere, e.g. nucleic acids, uric acid, worms, mites
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/06—Auxiliary integrated devices, integrated components
- B01L2300/0681—Filter
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/08—Geometry, shape and general structure
- B01L2300/0809—Geometry, shape and general structure rectangular shaped
- B01L2300/0816—Cards, e.g. flat sample carriers usually with flow in two horizontal directions
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/08—Geometry, shape and general structure
- B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
- B01L2300/088—Channel loops
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/18—Means for temperature control
- B01L2300/1805—Conductive heating, heat from thermostatted solids is conducted to receptacles, e.g. heating plates, blocks
- B01L2300/1822—Conductive heating, heat from thermostatted solids is conducted to receptacles, e.g. heating plates, blocks using Peltier elements
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/18—Means for temperature control
- B01L2300/1861—Means for temperature control using radiation
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/18—Means for temperature control
- B01L2300/1894—Cooling means; Cryo cooling
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2400/00—Moving or stopping fluids
- B01L2400/04—Moving fluids with specific forces or mechanical means
- B01L2400/0475—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure
- B01L2400/0487—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure fluid pressure, pneumatics
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- Immunology (AREA)
- Organic Chemistry (AREA)
- Biochemistry (AREA)
- Analytical Chemistry (AREA)
- Hematology (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Clinical Laboratory Science (AREA)
- Physics & Mathematics (AREA)
- Urology & Nephrology (AREA)
- Biomedical Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Genetics & Genomics (AREA)
- General Engineering & Computer Science (AREA)
- Dispersion Chemistry (AREA)
- Biophysics (AREA)
- Cell Biology (AREA)
- Medicinal Chemistry (AREA)
- Tropical Medicine & Parasitology (AREA)
- Pathology (AREA)
- Food Science & Technology (AREA)
- General Physics & Mathematics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Other Investigation Or Analysis Of Materials By Electrical Means (AREA)
- Peptides Or Proteins (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DE102014213783.7 | 2014-07-16 | ||
| DE102014213783.7A DE102014213783B3 (de) | 2014-07-16 | 2014-07-16 | Verfahren zur Identifizierung hochaffiner Komplexe aus zwei Liganden und einem Rezeptor, Vorrichtung zur Durchführung des Verfahrens und selbst-assemblierende chemische Bibliothek zur Verwendung in dem Verfahren |
| PCT/EP2015/065874 WO2016008822A2 (de) | 2014-07-16 | 2015-07-10 | Verfahren zur identifizierung hochaffiner komplexe aus zwei liganden und einem rezeptor, vorrichtung zur durchführung des verfahrens und selbst-assemblierende chemische bibliothek zur verwendung in dem verfahren |
Related Child Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2021013303A Division JP7112540B2 (ja) | 2014-07-16 | 2021-01-29 | ２個のリガンドと１個のレセプタとからなる高親和性錯体の特定方法と該方法を実行する装置及び該方法に用いられる自己集合型ケミカルライブラリ |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2017529062A JP2017529062A (ja) | 2017-10-05 |
| JP2017529062A5 JP2017529062A5 (enExample) | 2018-08-16 |
| JP6831776B2 true JP6831776B2 (ja) | 2021-02-17 |
Family
ID=53192981
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2017502621A Active JP6831776B2 (ja) | 2014-07-16 | 2015-07-10 | ２個のリガンドと１個のレセプタとからなる高親和性錯体の特定方法と該方法を実行する装置及び該方法に用いられる自己集合型ケミカルライブラリ |
| JP2021013303A Active JP7112540B2 (ja) | 2014-07-16 | 2021-01-29 | ２個のリガンドと１個のレセプタとからなる高親和性錯体の特定方法と該方法を実行する装置及び該方法に用いられる自己集合型ケミカルライブラリ |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2021013303A Active JP7112540B2 (ja) | 2014-07-16 | 2021-01-29 | ２個のリガンドと１個のレセプタとからなる高親和性錯体の特定方法と該方法を実行する装置及び該方法に用いられる自己集合型ケミカルライブラリ |
Country Status (8)
| Country | Link |
|---|---|
| US (2) | US11365439B2 (enExample) |
| EP (2) | EP3477299B1 (enExample) |
| JP (2) | JP6831776B2 (enExample) |
| CA (1) | CA2955087C (enExample) |
| DE (1) | DE102014213783B3 (enExample) |
| DK (1) | DK3169832T3 (enExample) |
| ES (2) | ES2712899T3 (enExample) |
| WO (1) | WO2016008822A2 (enExample) |
Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2017218293A1 (en) | 2016-06-16 | 2017-12-21 | Richard Edward Watts | Oligonucleotide directed and recorded combinatorial synthesis of encoded probe molecules |
| WO2018204420A1 (en) | 2017-05-02 | 2018-11-08 | Haystack Sciences Corporation | Molecules for verifying oligonucleotide directed combinatorial synthesis and methods of making and using the same |
| CN107652453B (zh) * | 2017-09-26 | 2020-05-05 | 天津工业大学 | 接枝rgd短肽温敏可注射水凝胶及其制备方法和应用 |
| EP3604525B1 (en) * | 2018-08-02 | 2021-03-10 | TU Dresden | Method for providing a dna-encoded library, dna-encoded library and method of decoding a dna-encoded library |
Family Cites Families (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5512439A (en) * | 1988-11-21 | 1996-04-30 | Dynal As | Oligonucleotide-linked magnetic particles and uses thereof |
| US7018793B1 (en) * | 1995-12-07 | 2006-03-28 | Diversa Corporation | Combinatorial screening of mixed populations of organisms |
| DE19619373A1 (de) * | 1996-05-14 | 1997-11-20 | Hoechst Ag | Neue Substanzbibliothek und damit hergestellte supramolekulare Komplexe |
| WO2002081729A2 (en) | 2001-04-06 | 2002-10-17 | California Institute Of Technology | Nucleic acid amplification utilizing microfluidic devices |
| KR100916889B1 (ko) * | 2002-03-08 | 2009-09-09 | 아이드게노쉬쉐 테흐니쉐 호흐슐레 쥬리히 | 암호화된 자가-조립 화학적 라이브러리(ｅｓａｃｈｅｌ) |
| CN101410530B (zh) * | 2003-04-18 | 2013-03-27 | 贝克顿·迪金森公司 | 免疫-扩增 |
| US7229769B2 (en) * | 2005-03-25 | 2007-06-12 | Illumina, Inc. | Compositions and methods for detecting protease activity |
| CA2664014A1 (en) * | 2006-09-18 | 2008-03-27 | Ensemble Discovery Corporation | Receptor family profiling |
| JP2009034052A (ja) * | 2007-08-02 | 2009-02-19 | Canon Inc | ハイブリダイゼーション方法および装置 |
| EP2138587A1 (en) * | 2008-06-23 | 2009-12-30 | Koninklijke Philips Electronics N.V. | Amplification of nucleic acids using temperature zones |
| CN102076870A (zh) | 2008-06-25 | 2011-05-25 | 实时基因组有限公司 | 对微阵列上的核酸进行解链曲线分析的方法和装置 |
| US8716191B2 (en) * | 2009-06-22 | 2014-05-06 | Universite De Strasbourg | Method of preparing an adduct |
| US10190986B2 (en) * | 2011-06-06 | 2019-01-29 | Abbott Laboratories | Spatially resolved ligand-receptor binding assays |
| BE1020816A5 (fr) * | 2012-07-04 | 2014-05-06 | Coris Bioconcept Sprl | Procede et dispositif pour la detection rapide de sequences nucleotidiques amplifiees. |
| CN105392897B (zh) * | 2013-03-19 | 2020-03-20 | 定向基因组学公司 | 靶序列的富集 |
-
2014
- 2014-07-16 DE DE102014213783.7A patent/DE102014213783B3/de active Active
-
2015
- 2015-07-10 JP JP2017502621A patent/JP6831776B2/ja active Active
- 2015-07-10 EP EP18207435.1A patent/EP3477299B1/de active Active
- 2015-07-10 US US15/326,172 patent/US11365439B2/en active Active
- 2015-07-10 ES ES15744501T patent/ES2712899T3/es active Active
- 2015-07-10 ES ES18207435T patent/ES3002709T3/es active Active
- 2015-07-10 WO PCT/EP2015/065874 patent/WO2016008822A2/de not_active Ceased
- 2015-07-10 CA CA2955087A patent/CA2955087C/en active Active
- 2015-07-10 EP EP15744501.6A patent/EP3169832B1/de active Active
- 2015-07-10 DK DK15744501.6T patent/DK3169832T3/en active
-
2021
- 2021-01-29 JP JP2021013303A patent/JP7112540B2/ja active Active
-
2022
- 2022-06-07 US US17/834,197 patent/US20220298552A1/en active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| EP3169832A2 (de) | 2017-05-24 |
| DE102014213783B3 (de) | 2015-06-18 |
| EP3477299C0 (de) | 2024-12-11 |
| CA2955087C (en) | 2023-03-07 |
| JP7112540B2 (ja) | 2022-08-03 |
| WO2016008822A2 (de) | 2016-01-21 |
| US11365439B2 (en) | 2022-06-21 |
| EP3477299B1 (de) | 2024-12-11 |
| JP2021092576A (ja) | 2021-06-17 |
| ES2712899T3 (es) | 2019-05-16 |
| US20170198336A1 (en) | 2017-07-13 |
| CA2955087A1 (en) | 2016-01-21 |
| DK3169832T3 (en) | 2019-03-25 |
| WO2016008822A3 (de) | 2016-05-19 |
| ES3002709T3 (en) | 2025-03-07 |
| EP3169832B1 (de) | 2018-12-05 |
| JP2017529062A (ja) | 2017-10-05 |
| US20220298552A1 (en) | 2022-09-22 |
| EP3477299A1 (de) | 2019-05-01 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| JP7112540B2 (ja) | ２個のリガンドと１個のレセプタとからなる高親和性錯体の特定方法と該方法を実行する装置及び該方法に用いられる自己集合型ケミカルライブラリ | |
| JP6525872B2 (ja) | 細胞中の複数のエピトープを同定するためのダイナミックレンジを高めること | |
| CN108779467B (zh) | 用于筛选核酸适配体的方法 | |
| US20210373000A1 (en) | Multipart reagents having increased avidity for polymerase binding | |
| US20200140944A1 (en) | Methods and systems for processing polynucleotides | |
| McGown et al. | The nucleic acid ligand. A new tool for molecular recognition | |
| JP6069224B2 (ja) | 細胞において複数のエピトープを同定する方法 | |
| Stoltenburg et al. | Capture‐SELEX: selection of DNA aptamers for aminoglycoside antibiotics | |
| JP2023099197A (ja) | 個々の細胞または細胞集団由来の核酸の分析方法 | |
| EP1483585B1 (en) | Encoded self-assembling chemical libraries (esachel) | |
| Zhang et al. | Molecularly imprinted polymers with DNA aptamer fragments as macromonomers | |
| WO2015047186A1 (en) | Methods to profile molecular complexes by using proximity bar-coding | |
| CN102321616B (zh) | 利用微流控芯片筛选蛋白质的核酸适体的方法 | |
| Nagano et al. | Accelerated Discovery of Potent Bioactive anti‐TNFα Aptamers by Microbead‐Assisted Capillary Electrophoresis (MACE)‐SELEX | |
| Le et al. | Determination of the Equilibrium Constant and Rate Constant of Protein–Oligonucleotide Complex Dissociation under the Conditions of Ideal-Filter Capillary Electrophoresis | |
| Pan et al. | Primer-free aptamer selection using a random DNA library | |
| CN102791883A (zh) | 筛选核酸配体的方法 | |
| JP2017529062A5 (enExample) | ||
| Acquah et al. | Chromatographic characterisation of aptamer-modified poly (EDMA-co-GMA) monolithic disk format for protein binding and separation | |
| Hong et al. | How does DNA ‘meet’capillary-based microsystems? | |
| Donovan et al. | Aptamer–drug conjugation for targeted tumor cell therapy | |
| Khodakov et al. | Sequence selective capture, release and analysis of DNA using a magnetic microbead-assisted toehold-mediated DNA strand displacement reaction | |
| JP2018170985A (ja) | 核酸分子およびその用途 | |
| JP2012143197A (ja) | Ｄｎａアプタマーの選択方法およびｄｎａアプタマー | |
| Meng | Custom Affinity Chromatography: Development of a Novel Platform for Rapid Creation and Validation of Affinity Media Using DNA Aptamer Based Ligands |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| A521 | Request for written amendment filed |
Free format text: JAPANESE INTERMEDIATE CODE: A523 Effective date: 20180706 |
|
| A621 | Written request for application examination |
Free format text: JAPANESE INTERMEDIATE CODE: A621 Effective date: 20180706 |
|
| A131 | Notification of reasons for refusal |
Free format text: JAPANESE INTERMEDIATE CODE: A131 Effective date: 20190917 |
|
| A521 | Request for written amendment filed |
Free format text: JAPANESE INTERMEDIATE CODE: A523 Effective date: 20191217 |
|
| A711 | Notification of change in applicant |
Free format text: JAPANESE INTERMEDIATE CODE: A711 Effective date: 20191217 |
|
| A521 | Request for written amendment filed |
Free format text: JAPANESE INTERMEDIATE CODE: A821 Effective date: 20191217 |
|
| A131 | Notification of reasons for refusal |
Free format text: JAPANESE INTERMEDIATE CODE: A131 Effective date: 20200512 |
|
| A521 | Request for written amendment filed |
Free format text: JAPANESE INTERMEDIATE CODE: A523 Effective date: 20200806 |
|
| TRDD | Decision of grant or rejection written | ||
| A01 | Written decision to grant a patent or to grant a registration (utility model) |
Free format text: JAPANESE INTERMEDIATE CODE: A01 Effective date: 20210105 |
|
| A61 | First payment of annual fees (during grant procedure) |
Free format text: JAPANESE INTERMEDIATE CODE: A61 Effective date: 20210129 |
|
| R150 | Certificate of patent or registration of utility model |
Ref document number: 6831776 Country of ref document: JP Free format text: JAPANESE INTERMEDIATE CODE: R150 |
|
| R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
| R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |