CA2955087C - Method for identifying high-affinity complexes of two ligands and a receptor using a self-assemblying chemical library of ligands - Google Patents

Method for identifying high-affinity complexes of two ligands and a receptor using a self-assemblying chemical library of ligands Download PDF

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Publication number
CA2955087C
CA2955087C CA2955087A CA2955087A CA2955087C CA 2955087 C CA2955087 C CA 2955087C CA 2955087 A CA2955087 A CA 2955087A CA 2955087 A CA2955087 A CA 2955087A CA 2955087 C CA2955087 C CA 2955087C
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CA
Canada
Prior art keywords
ligands
rna
bases
strand dna
complementary
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
CA2955087A
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English (en)
French (fr)
Other versions
CA2955087A1 (en
Inventor
Francesco Reddavide
Helena DE ANDRADE
Weilin LIN
Yixin Zhang
Elisa MELE
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Dynabind GmbH
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Dynabind GmbH
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Filing date
Publication date
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Publication of CA2955087A1 publication Critical patent/CA2955087A1/en
Application granted granted Critical
Publication of CA2955087C publication Critical patent/CA2955087C/en
Active legal-status Critical Current
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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6811—Selection methods for production or design of target specific oligonucleotides or binding molecules
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L7/00—Heating or cooling apparatus; Heat insulating devices
    • B01L7/52—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples
    • B01L7/525—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples with physical movement of samples between temperature zones
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/5308—Immunoassay; Biospecific binding assay; Materials therefor for analytes not provided for elsewhere, e.g. nucleic acids, uric acid, worms, mites
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/06—Auxiliary integrated devices, integrated components
    • B01L2300/0681—Filter
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0809—Geometry, shape and general structure rectangular shaped
    • B01L2300/0816—Cards, e.g. flat sample carriers usually with flow in two horizontal directions
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
    • B01L2300/088—Channel loops
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/18—Means for temperature control
    • B01L2300/1805—Conductive heating, heat from thermostatted solids is conducted to receptacles, e.g. heating plates, blocks
    • B01L2300/1822—Conductive heating, heat from thermostatted solids is conducted to receptacles, e.g. heating plates, blocks using Peltier elements
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/18—Means for temperature control
    • B01L2300/1861—Means for temperature control using radiation
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/18—Means for temperature control
    • B01L2300/1894—Cooling means; Cryo cooling
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0475—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure
    • B01L2400/0487—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure fluid pressure, pneumatics

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Organic Chemistry (AREA)
  • Biochemistry (AREA)
  • Analytical Chemistry (AREA)
  • Hematology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Clinical Laboratory Science (AREA)
  • Physics & Mathematics (AREA)
  • Biomedical Technology (AREA)
  • Urology & Nephrology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • General Engineering & Computer Science (AREA)
  • Dispersion Chemistry (AREA)
  • Biophysics (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Other Investigation Or Analysis Of Materials By Electrical Means (AREA)
  • Peptides Or Proteins (AREA)
CA2955087A 2014-07-16 2015-07-10 Method for identifying high-affinity complexes of two ligands and a receptor using a self-assemblying chemical library of ligands Active CA2955087C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
DE102014213783.7A DE102014213783B3 (de) 2014-07-16 2014-07-16 Verfahren zur Identifizierung hochaffiner Komplexe aus zwei Liganden und einem Rezeptor, Vorrichtung zur Durchführung des Verfahrens und selbst-assemblierende chemische Bibliothek zur Verwendung in dem Verfahren
DE102014213783.7 2014-07-16
PCT/EP2015/065874 WO2016008822A2 (de) 2014-07-16 2015-07-10 Verfahren zur identifizierung hochaffiner komplexe aus zwei liganden und einem rezeptor, vorrichtung zur durchführung des verfahrens und selbst-assemblierende chemische bibliothek zur verwendung in dem verfahren

Publications (2)

Publication Number Publication Date
CA2955087A1 CA2955087A1 (en) 2016-01-21
CA2955087C true CA2955087C (en) 2023-03-07

Family

ID=53192981

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2955087A Active CA2955087C (en) 2014-07-16 2015-07-10 Method for identifying high-affinity complexes of two ligands and a receptor using a self-assemblying chemical library of ligands

Country Status (8)

Country Link
US (2) US11365439B2 (enExample)
EP (2) EP3169832B1 (enExample)
JP (2) JP6831776B2 (enExample)
CA (1) CA2955087C (enExample)
DE (1) DE102014213783B3 (enExample)
DK (1) DK3169832T3 (enExample)
ES (2) ES2712899T3 (enExample)
WO (1) WO2016008822A2 (enExample)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP7283727B2 (ja) 2016-06-16 2023-05-30 ヘイスタック サイエンシィズ コーポレーション コードプローブ分子のコンビナトリアル合成が指令されかつ記録されたオリゴヌクレオチド
EP3619340A4 (en) 2017-05-02 2021-01-20 Haystack Sciences Corporation OLIGONUCLEOTID-DRIVEN COMBINATORY SYNTHESIS VERIFICATION MOLECULES AND ASSOCIATED MANUFACTURING AND USE PROCESSES
CN107652453B (zh) * 2017-09-26 2020-05-05 天津工业大学 接枝rgd短肽温敏可注射水凝胶及其制备方法和应用
EP3604525B1 (en) * 2018-08-02 2021-03-10 TU Dresden Method for providing a dna-encoded library, dna-encoded library and method of decoding a dna-encoded library

Family Cites Families (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5512439A (en) * 1988-11-21 1996-04-30 Dynal As Oligonucleotide-linked magnetic particles and uses thereof
US7018793B1 (en) * 1995-12-07 2006-03-28 Diversa Corporation Combinatorial screening of mixed populations of organisms
DE19619373A1 (de) * 1996-05-14 1997-11-20 Hoechst Ag Neue Substanzbibliothek und damit hergestellte supramolekulare Komplexe
WO2002081729A2 (en) 2001-04-06 2002-10-17 California Institute Of Technology Nucleic acid amplification utilizing microfluidic devices
DE60231436D1 (de) * 2002-03-08 2009-04-16 Eidgenoess Tech Hochschule Kodierte, selbst-assemblierende chemische bibliotheken (esachel)
EP1615948B1 (en) * 2003-04-18 2015-04-01 Becton Dickinson and Company Immuno-amplification
US7229769B2 (en) * 2005-03-25 2007-06-12 Illumina, Inc. Compositions and methods for detecting protease activity
EP2064348B1 (en) * 2006-09-18 2012-02-08 Ensemble Therapeutics Corporation Receptor family profiling
JP2009034052A (ja) * 2007-08-02 2009-02-19 Canon Inc ハイブリダイゼーション方法および装置
EP2138587A1 (en) * 2008-06-23 2009-12-30 Koninklijke Philips Electronics N.V. Amplification of nucleic acids using temperature zones
US9103785B2 (en) * 2008-06-25 2015-08-11 Emergence Genomics, Llc Method and apparatus for melting curve analysis of nucleic acids in microarray format
DK2446033T3 (da) * 2009-06-22 2014-01-27 Univ Strasbourg Fremgangsmåde til fremstilling af et addukt
US10190986B2 (en) * 2011-06-06 2019-01-29 Abbott Laboratories Spatially resolved ligand-receptor binding assays
BE1020816A5 (fr) 2012-07-04 2014-05-06 Coris Bioconcept Sprl Procede et dispositif pour la detection rapide de sequences nucleotidiques amplifiees.
US9567632B2 (en) * 2013-03-19 2017-02-14 New England Biolabs, Inc. Enrichment of target sequences

Also Published As

Publication number Publication date
JP7112540B2 (ja) 2022-08-03
US20220298552A1 (en) 2022-09-22
WO2016008822A2 (de) 2016-01-21
WO2016008822A3 (de) 2016-05-19
JP6831776B2 (ja) 2021-02-17
EP3169832A2 (de) 2017-05-24
US11365439B2 (en) 2022-06-21
ES3002709T3 (en) 2025-03-07
JP2021092576A (ja) 2021-06-17
EP3477299B1 (de) 2024-12-11
EP3477299A1 (de) 2019-05-01
CA2955087A1 (en) 2016-01-21
DE102014213783B3 (de) 2015-06-18
JP2017529062A (ja) 2017-10-05
US20170198336A1 (en) 2017-07-13
ES2712899T3 (es) 2019-05-16
EP3477299C0 (de) 2024-12-11
EP3169832B1 (de) 2018-12-05
DK3169832T3 (en) 2019-03-25

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