JP6704251B2 - Ｆａｄ２性能座および標的化切断を誘導することができる対応する標的部位特異的結合タンパク質 - Google Patents

Ｆａｄ２性能座および標的化切断を誘導することができる対応する標的部位特異的結合タンパク質 Download PDF

Info

Publication number
JP6704251B2
JP6704251B2 JP2015531202A JP2015531202A JP6704251B2 JP 6704251 B2 JP6704251 B2 JP 6704251B2 JP 2015531202 A JP2015531202 A JP 2015531202A JP 2015531202 A JP2015531202 A JP 2015531202A JP 6704251 B2 JP6704251 B2 JP 6704251B2
Authority
JP
Japan
Prior art keywords
fad2
gene
seq
nucleic acid
zfp
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
JP2015531202A
Other languages
English (en)
Japanese (ja)
Other versions
JP2015527082A (ja
JP2015527082A5 (enExample
Inventor
マイケル エインリー，ウィリアム
マイケル エインリー，ウィリアム
アール． ウェブ，スティーブン
アール． ウェブ，スティーブン
サミュエル，ポン
ワイ． ガスチン，ディミトリー
ワイ． ガスチン，ディミトリー
シー． ミラー，ジェフリー
シー． ミラー，ジェフリー
ツァン，レイ
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Sangamo Therapeutics Inc
Original Assignee
Sangamo Therapeutics Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Sangamo Therapeutics Inc filed Critical Sangamo Therapeutics Inc
Publication of JP2015527082A publication Critical patent/JP2015527082A/ja
Publication of JP2015527082A5 publication Critical patent/JP2015527082A5/ja
Application granted granted Critical
Publication of JP6704251B2 publication Critical patent/JP6704251B2/ja
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H5/00—Angiosperms, i.e. flowering plants, characterised by their plant parts; Angiosperms characterised otherwise than by their botanic taxonomy
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
    • A01H1/06—Processes for producing mutations, e.g. treatment with chemicals or with radiation
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
    • C07K14/4701—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals not used
    • C07K14/4702—Regulators; Modulating activity
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/66—General methods for inserting a gene into a vector to form a recombinant vector using cleavage and ligation; Use of non-functional linkers or adaptors, e.g. linkers containing the sequence for a restriction endonuclease
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/822—Reducing position variability, e.g. by the use of scaffold attachment region/matrix attachment region (SAR/MAR); Use of SAR/MAR to regulate gene expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8247—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving modified lipid metabolism, e.g. seed oil composition
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/04—Plant cells or tissues
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/10—Cells modified by introduction of foreign genetic material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y301/00—Hydrolases acting on ester bonds (3.1)
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H5/00—Angiosperms, i.e. flowering plants, characterised by their plant parts; Angiosperms characterised otherwise than by their botanic taxonomy
    • A01H5/10—Seeds
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H6/00—Angiosperms, i.e. flowering plants, characterised by their botanic taxonomy
    • A01H6/54—Leguminosae or Fabaceae, e.g. soybean, alfalfa or peanut
    • A01H6/542—Glycine max [soybean]
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/80—Fusion polypeptide containing a DNA binding domain, e.g. Lacl or Tet-repressor
    • C07K2319/81—Fusion polypeptide containing a DNA binding domain, e.g. Lacl or Tet-repressor containing a Zn-finger domain for DNA binding
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
    • Y02A40/146—Genetically Modified [GMO] plants, e.g. transgenic plants

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Mycology (AREA)
  • Nutrition Science (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Botany (AREA)
  • Developmental Biology & Embryology (AREA)
  • Environmental Sciences (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Toxicology (AREA)
  • Physiology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Peptides Or Proteins (AREA)
JP2015531202A 2012-09-07 2013-09-05 Ｆａｄ２性能座および標的化切断を誘導することができる対応する標的部位特異的結合タンパク質 Active JP6704251B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201261697886P 2012-09-07 2012-09-07
US61/697,886 2012-09-07
PCT/US2013/058299 WO2014039702A2 (en) 2012-09-07 2013-09-05 Fad2 performance loci and corresponding target site specific binding proteins capable of inducing targeted breaks

Related Child Applications (1)

Application Number Title Priority Date Filing Date
JP2020016485A Division JP2020096611A (ja) 2012-09-07 2020-02-03 Ｆａｄ２性能座および標的化切断を誘導することができる対応する標的部位特異的結合タンパク質

Publications (3)

Publication Number Publication Date
JP2015527082A JP2015527082A (ja) 2015-09-17
JP2015527082A5 JP2015527082A5 (enExample) 2016-09-23
JP6704251B2 true JP6704251B2 (ja) 2020-06-03

Family

ID=50237761

Family Applications (4)

Application Number Title Priority Date Filing Date
JP2015531202A Active JP6704251B2 (ja) 2012-09-07 2013-09-05 Ｆａｄ２性能座および標的化切断を誘導することができる対応する標的部位特異的結合タンパク質
JP2015531201A Expired - Fee Related JP6792943B2 (ja) 2012-09-07 2013-09-05 Ｆａｄ２性能座および標的化切断を誘導することができる対応する標的部位特異的結合タンパク質
JP2020016485A Withdrawn JP2020096611A (ja) 2012-09-07 2020-02-03 Ｆａｄ２性能座および標的化切断を誘導することができる対応する標的部位特異的結合タンパク質
JP2020025358A Withdrawn JP2020110150A (ja) 2012-09-07 2020-02-18 Ｆａｄ２性能座および標的化切断を誘導することができる対応する標的部位特異的結合タンパク質

Family Applications After (3)

Application Number Title Priority Date Filing Date
JP2015531201A Expired - Fee Related JP6792943B2 (ja) 2012-09-07 2013-09-05 Ｆａｄ２性能座および標的化切断を誘導することができる対応する標的部位特異的結合タンパク質
JP2020016485A Withdrawn JP2020096611A (ja) 2012-09-07 2020-02-03 Ｆａｄ２性能座および標的化切断を誘導することができる対応する標的部位特異的結合タンパク質
JP2020025358A Withdrawn JP2020110150A (ja) 2012-09-07 2020-02-18 Ｆａｄ２性能座および標的化切断を誘導することができる対応する標的部位特異的結合タンパク質

Country Status (20)

Country Link
US (5) US9963711B2 (enExample)
EP (4) EP2893023B1 (enExample)
JP (4) JP6704251B2 (enExample)
KR (2) KR102147005B1 (enExample)
CN (4) CN104780756B (enExample)
AR (2) AR092480A1 (enExample)
AU (2) AU2013312455B2 (enExample)
BR (1) BR112015004980B1 (enExample)
CA (2) CA2884084C (enExample)
CL (2) CL2015000561A1 (enExample)
HK (2) HK1213736A1 (enExample)
IL (2) IL237537B (enExample)
MX (2) MX352081B (enExample)
NZ (1) NZ705742A (enExample)
RU (2) RU2656159C2 (enExample)
TW (1) TWI667348B (enExample)
UA (1) UA118090C2 (enExample)
UY (3) UY35020A (enExample)
WO (2) WO2014039692A2 (enExample)
ZA (2) ZA201501397B (enExample)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2020096611A (ja) * 2012-09-07 2020-06-25 ダウ アグロサイエンシィズ エルエルシー Ｆａｄ２性能座および標的化切断を誘導することができる対応する標的部位特異的結合タンパク質

Families Citing this family (70)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2013066438A2 (en) 2011-07-22 2013-05-10 President And Fellows Of Harvard College Evaluation and improvement of nuclease cleavage specificity
UA116097C2 (uk) 2011-12-11 2018-02-12 Зе Стейт Оф Ізраел, Міністрі Оф Агрікалче Енд Руерал Девелопмент, Агрікалчерал Рісьоч Організейшн, (А.Р.О.), Волкані Сентре Спосіб модуляції провідності устячка рослини
GB201122458D0 (en) 2011-12-30 2012-02-08 Univ Wageningen Modified cascade ribonucleoproteins and uses thereof
FI3597749T3 (fi) 2012-05-25 2023-10-09 Univ California Menetelmiä ja koostumuksia rna-ohjattua kohde-dna-modifikaatiota varten ja rna-ohjattua transkription modulaatiota varten
US20150225734A1 (en) 2012-06-19 2015-08-13 Regents Of The University Of Minnesota Gene targeting in plants using dna viruses
CA2884162C (en) 2012-09-07 2020-12-29 Dow Agrosciences Llc Fad3 performance loci and corresponding target site specific binding proteins capable of inducing targeted breaks
UA119135C2 (uk) 2012-09-07 2019-05-10 ДАУ АГРОСАЙЄНСІЗ ЕлЕлСі Спосіб отримання трансгенної рослини
KR101656236B1 (ko) * 2012-10-23 2016-09-12 주식회사 툴젠 표적 DNA에 특이적인 가이드 RNA 및 Cas 단백질을 암호화하는 핵산 또는 Cas 단백질을 포함하는, 표적 DNA를 절단하기 위한 조성물 및 이의 용도
MX367031B (es) 2012-11-01 2019-08-02 Cellectis Plantas para la produccion de proteinas terapeuticas.
PT3363902T (pt) 2012-12-06 2019-12-19 Sigma Aldrich Co Llc Modificação e regulação de genoma baseadas em crispr
US20140178561A1 (en) 2012-12-21 2014-06-26 Cellectis Potatoes with reduced cold-induced sweetening
AU2014235794A1 (en) 2013-03-14 2015-10-22 Caribou Biosciences, Inc. Compositions and methods of nucleic acid-targeting nucleic acids
US10113162B2 (en) 2013-03-15 2018-10-30 Cellectis Modifying soybean oil composition through targeted knockout of the FAD2-1A/1B genes
EP2966983B1 (en) * 2013-03-15 2021-07-28 Cellectis Modifying soybean oil composition through targeted knockout of the fad2-1a/1b genes
US20150044192A1 (en) 2013-08-09 2015-02-12 President And Fellows Of Harvard College Methods for identifying a target site of a cas9 nuclease
US9388430B2 (en) 2013-09-06 2016-07-12 President And Fellows Of Harvard College Cas9-recombinase fusion proteins and uses thereof
US9737604B2 (en) 2013-09-06 2017-08-22 President And Fellows Of Harvard College Use of cationic lipids to deliver CAS9
US9340799B2 (en) 2013-09-06 2016-05-17 President And Fellows Of Harvard College MRNA-sensing switchable gRNAs
KR20230054509A (ko) 2013-11-07 2023-04-24 에디타스 메디신, 인코포레이티드 지배적인 gRNA를 이용하는 CRISPR-관련 방법 및 조성물
US11053481B2 (en) 2013-12-12 2021-07-06 President And Fellows Of Harvard College Fusions of Cas9 domains and nucleic acid-editing domains
CA2952906A1 (en) 2014-06-20 2015-12-23 Cellectis Potatoes with reduced granule-bound starch synthase
AU2015298571B2 (en) 2014-07-30 2020-09-03 President And Fellows Of Harvard College Cas9 proteins including ligand-dependent inteins
US9970030B2 (en) 2014-08-27 2018-05-15 Caribou Biosciences, Inc. Methods for increasing CAS9-mediated engineering efficiency
CN107250148B (zh) 2014-12-03 2021-04-16 安捷伦科技有限公司 具有化学修饰的指导rna
WO2016112351A1 (en) * 2015-01-09 2016-07-14 Bio-Rad Laboratories, Inc. Detection of genome editing
ES2884838T3 (es) 2015-04-06 2021-12-13 Univ Leland Stanford Junior ARN guía químicamente modificados para la regulación génica mediada por CRISPR/CAS
WO2016174119A1 (en) * 2015-04-28 2016-11-03 Bayer Cropscience Nv Brassica plants with modified seed oil composition
US10837024B2 (en) 2015-09-17 2020-11-17 Cellectis Modifying messenger RNA stability in plant transformations
US12043852B2 (en) 2015-10-23 2024-07-23 President And Fellows Of Harvard College Evolved Cas9 proteins for gene editing
EP4646936A3 (en) 2016-02-02 2025-11-19 Cellectis Modifying soybean oil composition through targeted knockout of the fad3a/b/c genes
US10767175B2 (en) 2016-06-08 2020-09-08 Agilent Technologies, Inc. High specificity genome editing using chemically modified guide RNAs
IL263595B2 (en) * 2016-06-20 2023-11-01 Keygene Nv A method for targeted modification of DNA in plant cells
KR20250103795A (ko) 2016-08-03 2025-07-07 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 아데노신 핵염기 편집제 및 그의 용도
CN109804066A (zh) 2016-08-09 2019-05-24 哈佛大学的校长及成员们 可编程cas9-重组酶融合蛋白及其用途
US11542509B2 (en) 2016-08-24 2023-01-03 President And Fellows Of Harvard College Incorporation of unnatural amino acids into proteins using base editing
WO2018045321A1 (en) * 2016-09-02 2018-03-08 North Carolina State University Methods and compositions for modification of plastid genomes
AU2017342543B2 (en) 2016-10-14 2024-06-27 President And Fellows Of Harvard College AAV delivery of nucleobase editors
CN109689693B (zh) * 2016-11-03 2022-06-28 深圳华大生命科学研究院 提高基因编辑效率的方法和系统
UY37482A (es) 2016-11-16 2018-05-31 Cellectis Métodos para alterar el contenido de aminoácidos en plantas mediante mutaciones de desplazamiento de marco
US20200208166A1 (en) * 2016-12-22 2020-07-02 Toolgen Incorporated Oleic Acid-Enriched Plant Body Having Genetically Modified FAD2 And Production Method Thereof
WO2018119359A1 (en) 2016-12-23 2018-06-28 President And Fellows Of Harvard College Editing of ccr5 receptor gene to protect against hiv infection
EP3592381A1 (en) 2017-03-09 2020-01-15 President and Fellows of Harvard College Cancer vaccine
EP3592853A1 (en) 2017-03-09 2020-01-15 President and Fellows of Harvard College Suppression of pain by gene editing
KR20190127797A (ko) 2017-03-10 2019-11-13 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 시토신에서 구아닌으로의 염기 편집제
CA3057192A1 (en) 2017-03-23 2018-09-27 President And Fellows Of Harvard College Nucleobase editors comprising nucleic acid programmable dna binding proteins
EP3615668B1 (en) 2017-04-25 2024-02-28 Cellectis Alfalfa with reduced lignin composition
WO2018209320A1 (en) 2017-05-12 2018-11-15 President And Fellows Of Harvard College Aptazyme-embedded guide rnas for use with crispr-cas9 in genome editing and transcriptional activation
CN107217070A (zh) * 2017-05-22 2017-09-29 广东省农业科学院作物研究所 一种基于TALENs基因编辑花生育种方法
CN111801345A (zh) 2017-07-28 2020-10-20 哈佛大学的校长及成员们 使用噬菌体辅助连续进化(pace)的进化碱基编辑器的方法和组合物
WO2019139645A2 (en) 2017-08-30 2019-07-18 President And Fellows Of Harvard College High efficiency base editors comprising gam
CN107502621B (zh) * 2017-09-22 2021-04-13 福建农林大学 一种快速检测体内dna末端连接的方法
CA3082251A1 (en) 2017-10-16 2019-04-25 The Broad Institute, Inc. Uses of adenosine base editors
EP3724214A4 (en) 2017-12-15 2021-09-01 The Broad Institute Inc. SYSTEMS AND METHODS FOR PREDICTING REPAIR RESULTS IN GENETIC ENGINEERING
US11390876B2 (en) 2018-03-09 2022-07-19 Pioneer Hi-Bred International, Inc. Compositions and methods for modification of fatty acids in soybean
US20210010013A1 (en) 2018-04-04 2021-01-14 Cibus Us Llc Fad2 genes and mutations
WO2019226953A1 (en) 2018-05-23 2019-11-28 The Broad Institute, Inc. Base editors and uses thereof
EP3829292A4 (en) * 2018-06-01 2022-06-08 Pioneer Hi-Bred International, Inc. SYSTEMS AND METHODS FOR EDITING THE BRASSICA GENOME
CA3109325A1 (en) 2018-08-17 2020-02-20 Amsilk Gmbh Use of a structural polypeptide for plant coating
CN109182373B (zh) * 2018-09-18 2020-10-09 武汉市农业科学院 一种利用基因编辑技术获得高油酸油菜的方法
US12281338B2 (en) 2018-10-29 2025-04-22 The Broad Institute, Inc. Nucleobase editors comprising GeoCas9 and uses thereof
EP3874049A1 (en) * 2018-10-31 2021-09-08 Pioneer Hi-Bred International Inc. <smallcaps/>? ? ?ochrobactrum? ? ? ? ?compositions and methods for-mediated gene editing
US20220007607A1 (en) * 2018-11-05 2022-01-13 University Of Tennessee Research Foundation High throughput protoplast isolation and transformation of plant cells from a novel leaf-based cell culture-derived system
US12351837B2 (en) 2019-01-23 2025-07-08 The Broad Institute, Inc. Supernegatively charged proteins and uses thereof
DE112020001306T5 (de) 2019-03-19 2022-01-27 Massachusetts Institute Of Technology Verfahren und zusammensetzungen zur editierung von nukleotidsequenzen
CN110004173B (zh) * 2019-04-08 2023-03-14 天津吉诺沃生物科技有限公司 一种获得非转基因耐储存鲜食枸杞的方法
US12473543B2 (en) 2019-04-17 2025-11-18 The Broad Institute, Inc. Adenine base editors with reduced off-target effects
US12435330B2 (en) 2019-10-10 2025-10-07 The Broad Institute, Inc. Methods and compositions for prime editing RNA
JP2023525304A (ja) 2020-05-08 2023-06-15 ザ ブロード インスティテュート，インコーポレーテッド 標的二本鎖ヌクレオチド配列の両鎖同時編集のための方法および組成物
US11884915B2 (en) 2021-09-10 2024-01-30 Agilent Technologies, Inc. Guide RNAs with chemical modification for prime editing
EP4482855A1 (en) * 2022-02-22 2025-01-01 Peter Biotherapeutics, Inc. Technologies for genetic modification

Family Cites Families (171)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US789538A (en) 1904-11-11 1905-05-09 Colin E Ham Dumb-bell.
US1173508A (en) 1913-10-27 1916-02-29 Oscar F Heartwell Jr Ironing-board.
US4178330A (en) 1977-08-29 1979-12-11 Velsicol Chemical Corporation Haloaryl halobenzenesulfonate flame retardants
US4179475A (en) 1978-05-11 1979-12-18 Phillips Petroleum Company Olefin feed in HF alkylation of isoparaffin with olefin
US4727028A (en) 1981-06-22 1988-02-23 Eli Lilly And Company Recombinant DNA cloning vectors and the eukaryotic and prokaryotic transformants thereof
US4693977A (en) 1982-08-23 1987-09-15 Queen's University At Kingston Enzyme immobilization for producing cephalosporin antibiotics
US4536475A (en) 1982-10-05 1985-08-20 Phytogen Plant vector
US4535060A (en) 1983-01-05 1985-08-13 Calgene, Inc. Inhibition resistant 5-enolpyruvyl-3-phosphoshikimate synthetase, production and use
US5352605A (en) 1983-01-17 1994-10-04 Monsanto Company Chimeric genes for transforming plant cells using viral promoters
NL8300698A (nl) 1983-02-24 1984-09-17 Univ Leiden Werkwijze voor het inbouwen van vreemd dna in het genoom van tweezaadlobbige planten; agrobacterium tumefaciens bacterien en werkwijze voor het produceren daarvan; planten en plantecellen met gewijzigde genetische eigenschappen; werkwijze voor het bereiden van chemische en/of farmaceutische produkten.
NZ207765A (en) 1983-04-15 1987-03-06 Lubrizol Genetics Inc Plant expression of transferred dna(t-dna)from plasmids associated with agrobacterium sp
US5428147A (en) 1983-04-15 1995-06-27 Mycogen Plant Science, Inc. Octopine T-DNA promoters
US5447858A (en) 1984-04-13 1995-09-05 Mycogen Plant Sciences, Inc. Heat shock promoter and gene
US5420034A (en) 1986-07-31 1995-05-30 Calgene, Inc. Seed-specific transcriptional regulation
US5753475A (en) 1985-01-17 1998-05-19 Calgene, Inc. Methods and compositions for regulated transcription and expression of heterologous genes
US4943674A (en) 1987-05-26 1990-07-24 Calgene, Inc. Fruit specific transcriptional factors
US4886937A (en) 1985-05-20 1989-12-12 North Carolina State University Method for transforming pine
US4940835A (en) 1985-10-29 1990-07-10 Monsanto Company Glyphosate-resistant plants
US4810648A (en) 1986-01-08 1989-03-07 Rhone Poulenc Agrochimie Haloarylnitrile degrading gene, its use, and cells containing the gene
ES2018274T5 (es) 1986-03-11 1996-12-16 Plant Genetic Systems Nv Celulas vegetales resistentes a los inhibidores de glutamina sintetasa, preparadas por ingenieria genetica.
US4975374A (en) 1986-03-18 1990-12-04 The General Hospital Corporation Expression of wild type and mutant glutamine synthetase in foreign hosts
US5015580A (en) 1987-07-29 1991-05-14 Agracetus Particle-mediated transformation of soybean plants and lines
ES2039474T3 (es) 1986-12-05 1993-10-01 Ciba-Geigy Ag Procedimineto mejorado para la transformacion de protoplastos vegetales.
US5359142A (en) 1987-01-13 1994-10-25 Monsanto Company Method for enhanced expression of a protein
US5322938A (en) 1987-01-13 1994-06-21 Monsanto Company DNA sequence for enhancing the efficiency of transcription
EP0333033A1 (en) 1988-03-09 1989-09-20 Meiji Seika Kaisha Ltd. Glutamine synthesis gene and glutamine synthetase
US5416011A (en) 1988-07-22 1995-05-16 Monsanto Company Method for soybean transformation and regeneration
US5302523A (en) 1989-06-21 1994-04-12 Zeneca Limited Transformation of plant cells
US5501967A (en) 1989-07-26 1996-03-26 Mogen International, N.V./Rijksuniversiteit Te Leiden Process for the site-directed integration of DNA into the genome of plants
US7705215B1 (en) 1990-04-17 2010-04-27 Dekalb Genetics Corporation Methods and compositions for the production of stably transformed, fertile monocot plants and cells thereof
US5550318A (en) 1990-04-17 1996-08-27 Dekalb Genetics Corporation Methods and compositions for the production of stably transformed, fertile monocot plants and cells thereof
US6051753A (en) 1989-09-07 2000-04-18 Calgene, Inc. Figwort mosaic virus promoter and uses
ATE196318T1 (de) 1989-10-31 2000-09-15 Monsanto Co Promotor für transgene pflanzen
US5641876A (en) 1990-01-05 1997-06-24 Cornell Research Foundation, Inc. Rice actin gene and promoter
US5484956A (en) 1990-01-22 1996-01-16 Dekalb Genetics Corporation Fertile transgenic Zea mays plant comprising heterologous DNA encoding Bacillus thuringiensis endotoxin
HU220773B1 (hu) 1990-01-22 2002-05-28 Dekalb Genetics Corporation Eljárás termő transzgenikus kukoricanövények előállítására
US6403865B1 (en) 1990-08-24 2002-06-11 Syngenta Investment Corp. Method of producing transgenic maize using direct transformation of commercially important genotypes
US5633435A (en) 1990-08-31 1997-05-27 Monsanto Company Glyphosate-tolerant 5-enolpyruvylshikimate-3-phosphate synthases
US5266317A (en) 1990-10-04 1993-11-30 University Of Georgia Research Foundation, Inc. Insect-specific paralytic neurotoxin genes for use in biological insect control: methods and compositions
US5384253A (en) 1990-12-28 1995-01-24 Dekalb Genetics Corporation Genetic transformation of maize cells by electroporation of cells pretreated with pectin degrading enzymes
GB9104617D0 (en) 1991-03-05 1991-04-17 Nickerson Int Seed Pest control
GB9115909D0 (en) 1991-07-23 1991-09-04 Nickerson Int Seed Recombinant dna
DK39692D0 (da) 1992-03-25 1992-03-25 Danisco Biologisk materiale
US5487994A (en) 1992-04-03 1996-01-30 The Johns Hopkins University Insertion and deletion mutants of FokI restriction endonuclease
US5436150A (en) 1992-04-03 1995-07-25 The Johns Hopkins University Functional domains in flavobacterium okeanokoities (foki) restriction endonuclease
US5356802A (en) 1992-04-03 1994-10-18 The Johns Hopkins University Functional domains in flavobacterium okeanokoites (FokI) restriction endonuclease
DE69334225D1 (de) 1992-07-07 2008-07-31 Japan Tobacco Inc Verfahren zur transformation einer monokotyledon pflanze
EP0652965A1 (en) 1992-07-27 1995-05-17 Pioneer Hi-Bred International, Inc. An improved method of agrobacterium-mediated transformation of cultured soybean cells
US5607914A (en) 1993-01-13 1997-03-04 Pioneer Hi-Bred International, Inc. Synthetic antimicrobial peptides
US6118047A (en) 1993-08-25 2000-09-12 Dekalb Genetic Corporation Anthranilate synthase gene and method of use thereof for conferring tryptophan overproduction
US5362865A (en) 1993-09-02 1994-11-08 Monsanto Company Enhanced expression in plants using non-translated leader sequences
US5580852A (en) 1993-12-17 1996-12-03 Pioneer Hi-Bred International, Inc. Derivatives of tachyplesin having inhibitory activity towards plant pathogenic fungi
AU704601B2 (en) 1994-01-18 1999-04-29 Scripps Research Institute, The Zinc finger protein derivatives and methods therefor
US6242568B1 (en) 1994-01-18 2001-06-05 The Scripps Research Institute Zinc finger protein derivatives and methods therefor
US6140466A (en) 1994-01-18 2000-10-31 The Scripps Research Institute Zinc finger protein derivatives and methods therefor
EP0781331B1 (en) 1994-08-20 2008-09-03 Gendaq Limited Improvements in or relating to binding proteins for recognition of dna
GB9824544D0 (en) 1998-11-09 1999-01-06 Medical Res Council Screening system
US5789538A (en) 1995-02-03 1998-08-04 Massachusetts Institute Of Technology Zinc finger proteins with high affinity new DNA binding specificities
US5994627A (en) 1995-03-31 1999-11-30 Common Wealth Scientific And Industrial Research Organisation Genetic sequences conferring nematode resistance in plants and uses therefor
US5639463A (en) 1995-05-23 1997-06-17 The Mennen Company Clear cosmetic stick composition
US5693512A (en) 1996-03-01 1997-12-02 The Ohio State Research Foundation Method for transforming plant tissue by sonication
US5850019A (en) 1996-08-06 1998-12-15 University Of Kentucky Research Foundation Promoter (FLt) for the full-length transcript of peanut chlorotic streak caulimovirus (PCLSV) and expression of chimeric genes in plants
US5925523A (en) 1996-08-23 1999-07-20 President & Fellows Of Harvard College Intraction trap assay, reagents and uses thereof
AU4208797A (en) 1996-09-05 1998-03-26 Unilever Plc Salt-inducible promoter derivable from a lactic acid bacterium, and its use in a lactic acid bacterium for production of a desired protein
WO1998027878A1 (en) 1996-12-20 1998-07-02 Fundação E.J. Zerbini A prosthesis for thoracostomy and method for its implantation
BR9807488A (pt) 1997-01-20 2000-03-21 Plant Genetic Systems Nv Promotores de planta induzidos por agentes patogênicos.
US5981840A (en) 1997-01-24 1999-11-09 Pioneer Hi-Bred International, Inc. Methods for agrobacterium-mediated transformation
GB9703369D0 (en) 1997-02-18 1997-04-09 Lindqvist Bjorn H Process
GB2338237B (en) 1997-02-18 2001-02-28 Actinova Ltd In vitro peptide or protein expression library
US5922564A (en) 1997-02-24 1999-07-13 Performance Plants, Inc. Phosphate-deficiency inducible promoter
US5925521A (en) 1997-03-31 1999-07-20 Incyte Pharmaceuticals, Inc. Human serine carboxypeptidase
GB9710807D0 (en) 1997-05-23 1997-07-23 Medical Res Council Nucleic acid binding proteins
GB9710809D0 (en) 1997-05-23 1997-07-23 Medical Res Council Nucleic acid binding proteins
EP0987936A4 (en) 1997-06-12 2001-03-14 Cargill Inc FATTY ACID DESATURASES AND MUTED SEQUENCES THEREOF
US6087166A (en) 1997-07-03 2000-07-11 Basf Aktiengesellschaft Transcriptional activators with graded transactivation potential
US6410248B1 (en) 1998-01-30 2002-06-25 Massachusetts Institute Of Technology General strategy for selecting high-affinity zinc finger proteins for diverse DNA target sites
WO1999042587A1 (en) 1998-02-20 1999-08-26 Zeneca Limited Pollen specific promoter
AU754376B2 (en) 1998-02-26 2002-11-14 Pioneer Hi-Bred International, Inc. Family of maize PR-1 genes and promoters
DE69920879T2 (de) 1998-02-26 2005-10-13 Pioneer Hi-Bred International, Inc. Konstitutive maispromotoren
WO1999045132A1 (en) 1998-03-02 1999-09-10 Massachusetts Institute Of Technology Poly zinc finger proteins with improved linkers
US6635806B1 (en) 1998-05-14 2003-10-21 Dekalb Genetics Corporation Methods and compositions for expression of transgenes in plants
US6307123B1 (en) 1998-05-18 2001-10-23 Dekalb Genetics Corporation Methods and compositions for transgene identification
US6140815A (en) 1998-06-17 2000-10-31 Dover Instrument Corporation High stability spin stand platform
JP2000083680A (ja) 1998-07-16 2000-03-28 Nippon Paper Industries Co Ltd 光誘導型プロモ―タ―の制御下に置かれた不定芽再分化遺伝子を選抜マ―カ―遺伝子とする植物への遺伝子導入方法及びこれに用いる植物への遺伝子導入用ベクタ―
US6140081A (en) 1998-10-16 2000-10-31 The Scripps Research Institute Zinc finger binding domains for GNN
US6599692B1 (en) 1999-09-14 2003-07-29 Sangamo Bioscience, Inc. Functional genomics using zinc finger proteins
US7070934B2 (en) 1999-01-12 2006-07-04 Sangamo Biosciences, Inc. Ligand-controlled regulation of endogenous gene expression
US6453242B1 (en) 1999-01-12 2002-09-17 Sangamo Biosciences, Inc. Selection of sites for targeting by zinc finger proteins and methods of designing zinc finger proteins to bind to preselected sites
US6534261B1 (en) 1999-01-12 2003-03-18 Sangamo Biosciences, Inc. Regulation of endogenous gene expression in cells using zinc finger proteins
AU2848800A (en) 1999-01-14 2000-08-01 Monsanto Technology Llc Soybean transformation method
US7030215B2 (en) 1999-03-24 2006-04-18 Sangamo Biosciences, Inc. Position dependent recognition of GNN nucleotide triplets by zinc fingers
US6794136B1 (en) 2000-11-20 2004-09-21 Sangamo Biosciences, Inc. Iterative optimization in the design of binding proteins
US6232526B1 (en) 1999-05-14 2001-05-15 Dekalb Genetics Corp. Maize A3 promoter and methods for use thereof
US6207879B1 (en) 1999-05-14 2001-03-27 Dekalb Genetics Corporation Maize RS81 promoter and methods for use thereof
US6429357B1 (en) 1999-05-14 2002-08-06 Dekalb Genetics Corp. Rice actin 2 promoter and intron and methods for use thereof
US6194636B1 (en) 1999-05-14 2001-02-27 Dekalb Genetics Corp. Maize RS324 promoter and methods for use thereof
US6677503B1 (en) 1999-06-23 2004-01-13 Pioneer Hi-Bred International, Inc. Sunflower anti-pathogene proteins and genes and their uses
AU776576B2 (en) 1999-12-06 2004-09-16 Sangamo Biosciences, Inc. Methods of using randomized libraries of zinc finger proteins for the identification of gene function
CA2390819A1 (en) 2000-01-21 2001-07-26 Pioneer Hi-Bred International, Inc. Novel root-preferred promoter elements and methods of use
JP5047437B2 (ja) 2000-02-08 2012-10-10 サンガモ バイオサイエンシーズ， インコーポレイテッド 薬物の発見のための細胞
US20020061512A1 (en) 2000-02-18 2002-05-23 Kim Jin-Soo Zinc finger domains and methods of identifying same
US6388170B1 (en) 2000-04-07 2002-05-14 University Of Kentucky Research Foundation Bidirectional promoters and methods related thereto
WO2001088197A2 (en) 2000-05-16 2001-11-22 Massachusetts Institute Of Technology Methods and compositions for interaction trap assays
JP2002060786A (ja) 2000-08-23 2002-02-26 Kao Corp 硬質表面用殺菌防汚剤
WO2005012515A2 (en) 2003-04-29 2005-02-10 Pioneer Hi-Bred International, Inc. Novel glyphosate-n-acetyltransferase (gat) genes
US6586251B2 (en) * 2000-10-31 2003-07-01 Regeneron Pharmaceuticals, Inc. Methods of modifying eukaryotic cells
US7067317B2 (en) 2000-12-07 2006-06-27 Sangamo Biosciences, Inc. Regulation of angiogenesis with zinc finger proteins
US7022826B2 (en) 2001-02-26 2006-04-04 The Regents Of The University Of California Non-oligomerizing fluorescent proteins
GB0108491D0 (en) 2001-04-04 2001-05-23 Gendaq Ltd Engineering zinc fingers
JP2005500061A (ja) 2001-08-20 2005-01-06 ザ スクリップス リサーチ インスティテュート Ｃｎｎについての亜鉛フィンガー結合ドメイン
AU2003235589A1 (en) * 2002-01-14 2003-07-30 Brookhaven Science Associates, Llc Modified fatty acid hydroxylase protein and genes
US7262054B2 (en) 2002-01-22 2007-08-28 Sangamo Biosciences, Inc. Zinc finger proteins for DNA binding and gene regulation in plants
WO2003087341A2 (en) 2002-01-23 2003-10-23 The University Of Utah Research Foundation Targeted chromosomal mutagenesis using zinc finger nucleases
US20030232410A1 (en) 2002-03-21 2003-12-18 Monika Liljedahl Methods and compositions for using zinc finger endonucleases to enhance homologous recombination
EP1484959A4 (en) 2002-03-21 2005-08-31 Monsanto Technology Llc NUCLEIC ACID CONSTRUCTS AND METHOD FOR PRODUCING MODIFIED SEED OIL COMPOSITIONS
US7361635B2 (en) 2002-08-29 2008-04-22 Sangamo Biosciences, Inc. Simultaneous modulation of multiple genes
JP2006502748A (ja) 2002-09-05 2006-01-26 カリフォルニア インスティテュート オブ テクノロジー 遺伝子ターゲッティングを誘発するキメラヌクレアーゼの使用方法
US20060246440A1 (en) * 2002-10-23 2006-11-02 Joung J K Methods for producing zinc finger proteins that bind to extended dna target sequences
WO2004070035A2 (en) * 2003-02-03 2004-08-19 Sungene Gmbh & Co. Kgaa Method for increasing efficiency of homologous recombination in plants
AU2004210965C1 (en) 2003-02-11 2014-10-16 Corteva Agriscience Llc Altered FAD2 and FAD3 genes in brassica and the molecular marker-assisted detection thereof
US7888121B2 (en) 2003-08-08 2011-02-15 Sangamo Biosciences, Inc. Methods and compositions for targeted cleavage and recombination
US8409861B2 (en) 2003-08-08 2013-04-02 Sangamo Biosciences, Inc. Targeted deletion of cellular DNA sequences
US8404458B2 (en) 2003-09-16 2013-03-26 The Rockefeller University Histone modifications as binary switches controlling gene expression
US7407776B2 (en) 2003-09-19 2008-08-05 Sangamo Biosciences, Inc. Engineered zinc finger proteins for regulation of gene expression
KR100537955B1 (ko) 2003-10-29 2005-12-20 학교법인고려중앙학원 꽃가루 특이적 유전자 발현 프로모터
US7972854B2 (en) 2004-02-05 2011-07-05 Sangamo Biosciences, Inc. Methods and compositions for targeted cleavage and recombination
CA2562193A1 (en) 2004-04-08 2005-10-27 Sangamo Biosciences, Inc. Treatment of neuropathic pain with zinc finger proteins
ES2315859T3 (es) 2004-04-08 2009-04-01 Sangamo Biosciences, Inc. Metodos y composiciones para tratar afecciones neuropaticas y neurodegenerativas.
SI2308976T1 (sl) 2004-04-30 2013-05-31 Dow Agrosciences Llc Novi geni z rezistenco na herbicide
EP1844153A2 (en) * 2005-01-28 2007-10-17 Bayer BioScience N.V. Brassica plant comprising a mutant fatty acid desaturase
EP1877583A2 (en) 2005-05-05 2008-01-16 Arizona Board of Regents on behalf of the Unversity of Arizona Sequence enabled reassembly (seer) - a novel method for visualizing specific dna sequences
WO2007014275A2 (en) 2005-07-26 2007-02-01 Sangamo Biosciences, Inc. Targeted integration and expression of exogenous nucleic acid sequences
EP2484202B1 (en) 2005-10-28 2017-06-07 Dow AgroSciences LLC Novel herbicide resistance genes
EP1806398A1 (en) 2006-01-04 2007-07-11 Monsanto S.A.S. Fad-2 mutants and high oleic plants
EP2213731B1 (en) 2006-05-25 2013-12-04 Sangamo BioSciences, Inc. Variant foki cleavage half-domains
EP1862551A1 (en) 2006-05-29 2007-12-05 Institut National De La Recherche Agronomique (Inra) Genetic markers for high oleic acid content in plants
WO2008021207A2 (en) 2006-08-11 2008-02-21 Dow Agrosciences Llc Zinc finger nuclease-mediated homologous recombination
EP2092068B1 (en) 2006-12-14 2014-10-08 Dow AgroSciences LLC Optimized non-canonical zinc finger proteins
EP1944375A1 (en) 2007-01-11 2008-07-16 Monsanto S.A.S. FAD2 mutants and high oleic acid plants
CA2692453C (en) 2007-07-12 2018-01-09 Trevor Collingwood Methods and compositions for inactivating alpha 1,6 fucosyltransferase (fut8) gene expression
ES2585157T3 (es) 2007-09-27 2016-10-04 Dow Agrosciences Llc Proteínas de dedo de cinc de diseño que se dirigen a los genes de 5-enolpiruvil shikimato-3-fosfato sintasa
EP2188384B1 (en) 2007-09-27 2015-07-15 Sangamo BioSciences, Inc. Rapid in vivo identification of biologically active nucleases
AU2008317354B2 (en) 2007-10-25 2014-04-10 Ospedale San Raffaele S.R.L. Methods and compositions for targeted integration
US9418219B2 (en) 2008-04-11 2016-08-16 Microsoft Technology Licensing, Llc Inter-process message security
WO2009131632A1 (en) 2008-04-14 2009-10-29 Sangamo Biosciences, Inc. Linear donor constructs for targeted integration
JP2011519558A (ja) * 2008-04-28 2011-07-14 プレシジョン バイオサイエンシズ，インク． 合理的に設計されたｄｎａ結合タンパク質とエフェクタードメインとの融合分子
KR20160015400A (ko) 2008-08-22 2016-02-12 상가모 바이오사이언스 인코포레이티드 표적화된 단일가닥 분할 및 표적화된 통합을 위한 방법 및 조성물
SI2370575T1 (en) 2008-12-17 2018-07-31 Dow Agrosciences, Llc Targeted integration in the Zp15 locus
US20110239315A1 (en) 2009-01-12 2011-09-29 Ulla Bonas Modular dna-binding domains and methods of use
EP2206723A1 (en) 2009-01-12 2010-07-14 Bonas, Ulla Modular DNA-binding domains
US9474299B2 (en) * 2009-06-22 2016-10-25 Saga University Mutation that increases the oleic acid content in soybean oil and responsible gene thereof
US9035129B2 (en) * 2009-07-08 2015-05-19 The Curators Of The University Of Missouri Method to develop high oleic acid soybeans using conventional soybean breeding techniques
KR20170125406A (ko) 2009-08-11 2017-11-14 상가모 테라퓨틱스, 인코포레이티드 표적화 변형에 대한 동형접합성 유기체
US10340778B2 (en) 2009-10-19 2019-07-02 Qm Power, Inc. Parallel magnetic circuit motor
RU2712833C2 (ru) * 2009-10-22 2020-01-31 ДАУ АГРОСАЙЕНСИЗ ЭлЭлСи Конструируемые белки с цинковыми пальцами, направленные на гены растений, вовлеченные в биосинтез жирных кислот
CA2783351C (en) 2009-12-10 2021-09-07 Regents Of The University Of Minnesota Tal effector-mediated dna modification
HUE033500T2 (en) 2010-01-22 2017-12-28 Dow Agrosciences Llc Engineered landing pads for gene targeting in plants
EP2526112B1 (en) 2010-01-22 2018-10-17 Dow AgroSciences LLC Targeted genomic alteration
ES2751916T3 (es) 2010-02-08 2020-04-02 Sangamo Therapeutics Inc Semidominios de escisión genomanipulados
WO2011100058A1 (en) 2010-02-09 2011-08-18 Sangamo Biosciences, Inc. Targeted genomic modification with partially single-stranded donor molecules
US8658355B2 (en) 2010-05-17 2014-02-25 The Uab Research Foundation General mass spectrometry assay using continuously eluting co-fractionating reporters of mass spectrometry detection efficiency
CA2798988C (en) 2010-05-17 2020-03-10 Sangamo Biosciences, Inc. Tal-effector (tale) dna-binding polypeptides and uses thereof
SG10201504197SA (en) 2010-05-28 2015-06-29 Solazyme Inc Food Compositions Comprising Tailored Oils
CN107119060A (zh) 2012-02-01 2017-09-01 陶氏益农公司 草甘膦抗性植物和相关方法
BR112014027813A2 (pt) 2012-05-07 2017-08-08 Dow Agrosciences Llc métodos e composições para integração de transgenes direcionada mediada por nuclease
US8897552B2 (en) * 2012-08-01 2014-11-25 Microsoft Corporation Setting an operating-system color using a photograph
PL3447958T3 (pl) * 2012-08-03 2020-11-16 Telefonaktiebolaget Lm Ericsson (Publ) Projekt przestrzeni wyszukiwania EPDCCH
UA118090C2 (uk) * 2012-09-07 2018-11-26 ДАУ АГРОСАЙЄНСІЗ ЕлЕлСі Спосіб інтегрування послідовності нуклеїнової кислоти, що представляє інтерес, у ген fad2 у клітині сої та специфічний для локусу fad2 білок, що зв'язується, здатний індукувати спрямований розрив
EP2966983B1 (en) * 2013-03-15 2021-07-28 Cellectis Modifying soybean oil composition through targeted knockout of the fad2-1a/1b genes
US9417070B1 (en) 2013-04-01 2016-08-16 Nextgen Aerosciences, Inc. Systems and methods for continuous replanning of vehicle trajectories
JP5937635B2 (ja) 2014-03-28 2016-06-22 ファナック株式会社 電磁接触器の溶着検出機能を有するモータ駆動装置

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2020096611A (ja) * 2012-09-07 2020-06-25 ダウ アグロサイエンシィズ エルエルシー Ｆａｄ２性能座および標的化切断を誘導することができる対応する標的部位特異的結合タンパク質

Also Published As

Publication number Publication date
IL237537A0 (en) 2015-04-30
WO2014039692A3 (en) 2015-07-16
US9493779B2 (en) 2016-11-15
JP2015527082A (ja) 2015-09-17
US9963711B2 (en) 2018-05-08
US10844389B2 (en) 2020-11-24
EP2892321A4 (en) 2016-03-02
US20140090116A1 (en) 2014-03-27
CA2883846A1 (en) 2014-03-13
AU2013312465A1 (en) 2015-03-12
EP2892321A2 (en) 2015-07-15
US10287595B2 (en) 2019-05-14
HK1213736A1 (zh) 2016-07-15
UY35021A (es) 2014-03-31
NZ705742A (en) 2018-11-30
RU2015112584A (ru) 2016-10-27
CN104780756A (zh) 2015-07-15
CA2884084A1 (en) 2014-03-13
ZA201501396B (en) 2016-06-29
CL2015000563A1 (es) 2015-10-02
UY35020A (es) 2014-03-31
MX352081B (es) 2017-11-08
AU2013312455A1 (en) 2015-03-12
UY39946A (es) 2022-10-31
UA118090C2 (uk) 2018-11-26
AU2013312465B2 (en) 2018-08-09
JP2020096611A (ja) 2020-06-25
ZA201501397B (en) 2016-06-29
CN105025702A (zh) 2015-11-04
TWI667348B (zh) 2019-08-01
AR092481A1 (es) 2015-04-22
HK1212153A1 (en) 2016-06-10
JP2020110150A (ja) 2020-07-27
EP2893023A4 (en) 2016-09-21
JP2015529464A (ja) 2015-10-08
RU2656158C2 (ru) 2018-05-31
RU2656159C2 (ru) 2018-05-31
AU2013312455B2 (en) 2018-12-13
MX2015002990A (es) 2015-09-28
KR20150043539A (ko) 2015-04-22
AU2013312465A2 (en) 2015-03-12
US10577616B2 (en) 2020-03-03
US20180223297A1 (en) 2018-08-09
EP2893023A2 (en) 2015-07-15
CN108610408A (zh) 2018-10-02
CN108610408B (zh) 2023-04-07
WO2014039702A2 (en) 2014-03-13
KR102147005B1 (ko) 2020-08-21
BR112015004980A2 (pt) 2017-11-21
AU2013312455A2 (en) 2018-12-13
KR102147006B1 (ko) 2020-08-21
JP6792943B2 (ja) 2020-12-02
EP3431600B1 (en) 2024-02-14
IL237536A0 (en) 2015-04-30
EP3404099B1 (en) 2024-01-10
WO2014039702A3 (en) 2014-07-24
BR112015004948A2 (pt) 2017-11-21
IL237537B (en) 2020-05-31
IL237536B (en) 2019-02-28
CA2884084C (en) 2021-10-26
CN104780756B (zh) 2018-10-02
CA2883846C (en) 2021-10-19
EP3404099A1 (en) 2018-11-21
EP2893023B1 (en) 2018-08-15
MX369701B (es) 2019-11-19
KR20150043538A (ko) 2015-04-22
RU2015112583A (ru) 2016-10-27
US20170044559A1 (en) 2017-02-16
WO2014039692A2 (en) 2014-03-13
TW201414838A (zh) 2014-04-16
US20140090112A1 (en) 2014-03-27
AR092480A1 (es) 2015-04-22
US20190316141A1 (en) 2019-10-17
EP3431600A1 (en) 2019-01-23
EP2892321B1 (en) 2018-10-24
CL2015000561A1 (es) 2015-10-02
BR112015004980B1 (pt) 2023-04-11
CN110684806A (zh) 2020-01-14

Similar Documents

Publication Publication Date Title
JP6704251B2 (ja) Ｆａｄ２性能座および標的化切断を誘導することができる対応する標的部位特異的結合タンパク質
JP6775953B2 (ja) Ｆａｄ３性能座および標的化切断を誘導可能である対応する標的部位特異的結合タンパク質
BR112015004948B1 (pt) Método para modificação de um gene fad2 em uma célula de soja, usos de célula, semente ou planta de soja e nuclease de dedo de zinco sítio- específica

Legal Events

Date Code Title Description
A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20160802

A621 Written request for application examination

Free format text: JAPANESE INTERMEDIATE CODE: A621

Effective date: 20160802

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20170523

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20170822

A02 Decision of refusal

Free format text: JAPANESE INTERMEDIATE CODE: A02

Effective date: 20180109

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20180508

A911 Transfer to examiner for re-examination before appeal (zenchi)

Free format text: JAPANESE INTERMEDIATE CODE: A911

Effective date: 20180704

A912 Re-examination (zenchi) completed and case transferred to appeal board

Free format text: JAPANESE INTERMEDIATE CODE: A912

Effective date: 20180831

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20200203

A61 First payment of annual fees (during grant procedure)

Free format text: JAPANESE INTERMEDIATE CODE: A61

Effective date: 20200512

R150 Certificate of patent or registration of utility model

Ref document number: 6704251

Country of ref document: JP

Free format text: JAPANESE INTERMEDIATE CODE: R150

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250