JP6623406B2 - 結合剤 - Google Patents
結合剤 Download PDFInfo
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- JP6623406B2 JP6623406B2 JP2015158726A JP2015158726A JP6623406B2 JP 6623406 B2 JP6623406 B2 JP 6623406B2 JP 2015158726 A JP2015158726 A JP 2015158726A JP 2015158726 A JP2015158726 A JP 2015158726A JP 6623406 B2 JP6623406 B2 JP 6623406B2
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- metal
- binding
- oass
- amino acid
- acid sequence
- Prior art date
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- 239000011230 binding agent Substances 0.000 title claims description 44
- 229910052751 metal Inorganic materials 0.000 claims description 128
- 239000002184 metal Substances 0.000 claims description 128
- 108090000765 processed proteins & peptides Proteins 0.000 claims description 70
- 108090000623 proteins and genes Proteins 0.000 claims description 61
- KDLHZDBZIXYQEI-UHFFFAOYSA-N Palladium Chemical compound [Pd] KDLHZDBZIXYQEI-UHFFFAOYSA-N 0.000 claims description 47
- 102000004169 proteins and genes Human genes 0.000 claims description 43
- 102000014914 Carrier Proteins Human genes 0.000 claims description 28
- 108091008324 binding proteins Proteins 0.000 claims description 28
- 102000004190 Enzymes Human genes 0.000 claims description 13
- 108090000790 Enzymes Proteins 0.000 claims description 13
- 210000004899 c-terminal region Anatomy 0.000 claims description 12
- 229910052763 palladium Inorganic materials 0.000 claims description 12
- 238000005259 measurement Methods 0.000 claims description 11
- 238000000034 method Methods 0.000 claims description 11
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 7
- 108090000854 Oxidoreductases Proteins 0.000 claims description 6
- 102000004316 Oxidoreductases Human genes 0.000 claims description 6
- 239000008103 glucose Substances 0.000 claims description 6
- 239000003153 chemical reaction reagent Substances 0.000 claims description 4
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- 125000002791 glucosyl group Chemical group C1([C@H](O)[C@@H](O)[C@H](O)[C@H](O1)CO)* 0.000 claims description 2
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- 125000003275 alpha amino acid group Chemical group 0.000 claims 1
- 150000001413 amino acids Chemical class 0.000 description 101
- 229910052700 potassium Inorganic materials 0.000 description 50
- 108010050375 Glucose 1-Dehydrogenase Proteins 0.000 description 24
- 101710138316 O-acetylserine sulfhydrylase Proteins 0.000 description 21
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- 239000013598 vector Substances 0.000 description 19
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- 229910052720 vanadium Inorganic materials 0.000 description 10
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- 239000007788 liquid Substances 0.000 description 7
- BASFCYQUMIYNBI-UHFFFAOYSA-N platinum Chemical group [Pt] BASFCYQUMIYNBI-UHFFFAOYSA-N 0.000 description 7
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 6
- 238000010586 diagram Methods 0.000 description 6
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- 230000003100 immobilizing effect Effects 0.000 description 4
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- AVKUERGKIZMTKX-NJBDSQKTSA-N ampicillin Chemical compound C1([C@@H](N)C(=O)N[C@H]2[C@H]3SC([C@@H](N3C2=O)C(O)=O)(C)C)=CC=CC=C1 AVKUERGKIZMTKX-NJBDSQKTSA-N 0.000 description 2
- 229960000723 ampicillin Drugs 0.000 description 2
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- HVYWMOMLDIMFJA-DPAQBDIFSA-N cholesterol Chemical compound C1C=C2C[C@@H](O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H]([C@H](C)CCCC(C)C)[C@@]1(C)CC2 HVYWMOMLDIMFJA-DPAQBDIFSA-N 0.000 description 2
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- 230000002779 inactivation Effects 0.000 description 2
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 description 2
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- 239000000256 polyoxyethylene sorbitan monolaurate Substances 0.000 description 2
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- YBADLXQNJCMBKR-UHFFFAOYSA-N (4-nitrophenyl)acetic acid Chemical compound OC(=O)CC1=CC=C([N+]([O-])=O)C=C1 YBADLXQNJCMBKR-UHFFFAOYSA-N 0.000 description 1
- CCBICDLNWJRFPO-UHFFFAOYSA-N 2,6-dichloroindophenol Chemical compound C1=CC(O)=CC=C1N=C1C=C(Cl)C(=O)C(Cl)=C1 CCBICDLNWJRFPO-UHFFFAOYSA-N 0.000 description 1
- 108010021809 Alcohol dehydrogenase Proteins 0.000 description 1
- 108010025188 Alcohol oxidase Proteins 0.000 description 1
- 239000004475 Arginine Substances 0.000 description 1
- 241000235349 Ascomycota Species 0.000 description 1
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- 235000014469 Bacillus subtilis Nutrition 0.000 description 1
- 241000221198 Basidiomycota Species 0.000 description 1
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 1
- 108010089254 Cholesterol oxidase Proteins 0.000 description 1
- 101710088194 Dehydrogenase Proteins 0.000 description 1
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 1
- 241000233866 Fungi Species 0.000 description 1
- 108010035289 Glucose Dehydrogenases Proteins 0.000 description 1
- 102000003886 Glycoproteins Human genes 0.000 description 1
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- 241000238631 Hexapoda Species 0.000 description 1
- 108700023483 L-lactate dehydrogenases Proteins 0.000 description 1
- 108010073450 Lactate 2-monooxygenase Proteins 0.000 description 1
- 239000004472 Lysine Substances 0.000 description 1
- KDXKERNSBIXSRK-UHFFFAOYSA-N Lysine Natural products NCCCCC(N)C(O)=O KDXKERNSBIXSRK-UHFFFAOYSA-N 0.000 description 1
- 108010058846 Ovalbumin Proteins 0.000 description 1
- 108010067902 Peptide Library Proteins 0.000 description 1
- 101800004192 Peptide P1 Proteins 0.000 description 1
- PCNDJXKNXGMECE-UHFFFAOYSA-N Phenazine Natural products C1=CC=CC2=NC3=CC=CC=C3N=C21 PCNDJXKNXGMECE-UHFFFAOYSA-N 0.000 description 1
- KJTLSVCANCCWHF-UHFFFAOYSA-N Ruthenium Chemical compound [Ru] KJTLSVCANCCWHF-UHFFFAOYSA-N 0.000 description 1
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 1
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- 150000001341 alkaline earth metal compounds Chemical class 0.000 description 1
- 150000003863 ammonium salts Chemical class 0.000 description 1
- 238000005349 anion exchange Methods 0.000 description 1
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- 239000012472 biological sample Substances 0.000 description 1
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- 235000012000 cholesterol Nutrition 0.000 description 1
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- 239000005515 coenzyme Substances 0.000 description 1
- 238000004040 coloring Methods 0.000 description 1
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- 239000000470 constituent Substances 0.000 description 1
- 239000003431 cross linking reagent Substances 0.000 description 1
- LOKCTEFSRHRXRJ-UHFFFAOYSA-I dipotassium trisodium dihydrogen phosphate hydrogen phosphate dichloride Chemical compound P(=O)(O)(O)[O-].[K+].P(=O)(O)([O-])[O-].[Na+].[Na+].[Cl-].[K+].[Cl-].[Na+] LOKCTEFSRHRXRJ-UHFFFAOYSA-I 0.000 description 1
- 235000019441 ethanol Nutrition 0.000 description 1
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- VWWQXMAJTJZDQX-UYBVJOGSSA-N flavin adenine dinucleotide Chemical compound C1=NC2=C(N)N=CN=C2N1[C@@H]([C@H](O)[C@@H]1O)O[C@@H]1CO[P@](O)(=O)O[P@@](O)(=O)OC[C@@H](O)[C@@H](O)[C@@H](O)CN1C2=NC(=O)NC(=O)C2=NC2=C1C=C(C)C(C)=C2 VWWQXMAJTJZDQX-UYBVJOGSSA-N 0.000 description 1
- 235000019162 flavin adenine dinucleotide Nutrition 0.000 description 1
- 239000011714 flavin adenine dinucleotide Substances 0.000 description 1
- 229940093632 flavin-adenine dinucleotide Drugs 0.000 description 1
- 229910052731 fluorine Inorganic materials 0.000 description 1
- 235000001727 glucose Nutrition 0.000 description 1
- 150000003278 haem Chemical class 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 229910052739 hydrogen Inorganic materials 0.000 description 1
- 229910052740 iodine Inorganic materials 0.000 description 1
- 150000002500 ions Chemical class 0.000 description 1
- 229910052741 iridium Inorganic materials 0.000 description 1
- GKOZUEZYRPOHIO-UHFFFAOYSA-N iridium atom Chemical compound [Ir] GKOZUEZYRPOHIO-UHFFFAOYSA-N 0.000 description 1
- 235000014655 lactic acid Nutrition 0.000 description 1
- 239000004310 lactic acid Substances 0.000 description 1
- 210000004962 mammalian cell Anatomy 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- JZMJDSHXVKJFKW-UHFFFAOYSA-M methyl sulfate(1-) Chemical compound COS([O-])(=O)=O JZMJDSHXVKJFKW-UHFFFAOYSA-M 0.000 description 1
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- 150000002908 osmium compounds Chemical class 0.000 description 1
- 229940092253 ovalbumin Drugs 0.000 description 1
- 238000007254 oxidation reaction Methods 0.000 description 1
- AZQWKYJCGOJGHM-UHFFFAOYSA-N para-benzoquinone Natural products O=C1C=CC(=O)C=C1 AZQWKYJCGOJGHM-UHFFFAOYSA-N 0.000 description 1
- 238000002823 phage display Methods 0.000 description 1
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- 229910052698 phosphorus Inorganic materials 0.000 description 1
- 239000013612 plasmid Substances 0.000 description 1
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- 239000010453 quartz Substances 0.000 description 1
- 229910052703 rhodium Inorganic materials 0.000 description 1
- 239000010948 rhodium Substances 0.000 description 1
- MHOVAHRLVXNVSD-UHFFFAOYSA-N rhodium atom Chemical compound [Rh] MHOVAHRLVXNVSD-UHFFFAOYSA-N 0.000 description 1
- 229910052707 ruthenium Inorganic materials 0.000 description 1
- 238000007650 screen-printing Methods 0.000 description 1
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N silicon dioxide Inorganic materials O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 108010085346 steroid delta-isomerase Proteins 0.000 description 1
- 150000005846 sugar alcohols Chemical class 0.000 description 1
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- 150000003467 sulfuric acid derivatives Chemical class 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
- 210000001138 tear Anatomy 0.000 description 1
- 238000006257 total synthesis reaction Methods 0.000 description 1
- 229910052721 tungsten Inorganic materials 0.000 description 1
- 210000002700 urine Anatomy 0.000 description 1
Images
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- Investigating Or Analysing Biological Materials (AREA)
- Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
- Enzymes And Modification Thereof (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
Priority Applications (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP2015158726A JP6623406B2 (ja) | 2015-08-11 | 2015-08-11 | 結合剤 |
PCT/JP2016/073537 WO2017026505A1 (ja) | 2015-08-11 | 2016-08-10 | 結合剤 |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP2015158726A JP6623406B2 (ja) | 2015-08-11 | 2015-08-11 | 結合剤 |
Publications (3)
Publication Number | Publication Date |
---|---|
JP2017037014A JP2017037014A (ja) | 2017-02-16 |
JP2017037014A5 JP2017037014A5 (enrdf_load_stackoverflow) | 2018-10-04 |
JP6623406B2 true JP6623406B2 (ja) | 2019-12-25 |
Family
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Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
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JP2015158726A Active JP6623406B2 (ja) | 2015-08-11 | 2015-08-11 | 結合剤 |
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JP (1) | JP6623406B2 (enrdf_load_stackoverflow) |
Families Citing this family (3)
Publication number | Priority date | Publication date | Assignee | Title |
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KR101970327B1 (ko) * | 2017-04-13 | 2019-04-18 | 고려대학교 산학협력단 | 검출 신호의 자가 증폭 원리를 이용한 정확, 신속, 편리한 단일 단계 질병 진단 방법 |
EP4563699A1 (en) | 2022-07-26 | 2025-06-04 | Panasonic Intellectual Property Management Co., Ltd. | Enzyme electrode, enzymatic reaction device, enzymatic reaction method, modified protein, and production method of modified protein |
EP4563698A1 (en) | 2022-07-26 | 2025-06-04 | Panasonic Intellectual Property Management Co., Ltd. | Enzyme electrode, reduction device, reduction method, modified protein, and production method for modified protein |
Family Cites Families (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20060246426A1 (en) * | 2003-09-26 | 2006-11-02 | Biohesion, Inc. | Recombinant fusion proteins with high affinity binding to gold and applications thereof |
WO2011062263A1 (ja) * | 2009-11-20 | 2011-05-26 | 財団法人東京都医学研究機構 | 医薬組成物 |
WO2013143026A1 (en) * | 2012-03-31 | 2013-10-03 | Abmart (Shanghai) Co., Ltd | Peptide and antibody libraries and uses thereof |
JP2014205626A (ja) * | 2013-04-11 | 2014-10-30 | 学校法人東京薬科大学 | 白金結合剤、及びその利用 |
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2015
- 2015-08-11 JP JP2015158726A patent/JP6623406B2/ja active Active
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