JP6590366B2 - 顕微鏡装置、オートフォーカス装置、及び、オートフォーカス方法 - Google Patents
顕微鏡装置、オートフォーカス装置、及び、オートフォーカス方法 Download PDFInfo
- Publication number
- JP6590366B2 JP6590366B2 JP2015188906A JP2015188906A JP6590366B2 JP 6590366 B2 JP6590366 B2 JP 6590366B2 JP 2015188906 A JP2015188906 A JP 2015188906A JP 2015188906 A JP2015188906 A JP 2015188906A JP 6590366 B2 JP6590366 B2 JP 6590366B2
- Authority
- JP
- Japan
- Prior art keywords
- optical axis
- autofocus
- objective lens
- light
- microscope apparatus
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 238000000034 method Methods 0.000 title claims description 19
- 230000003287 optical effect Effects 0.000 claims description 148
- 238000005286 illumination Methods 0.000 claims description 66
- 238000001514 detection method Methods 0.000 claims description 6
- 230000001678 irradiating effect Effects 0.000 claims description 2
- 230000002411 adverse Effects 0.000 description 9
- 238000012545 processing Methods 0.000 description 8
- 230000000694 effects Effects 0.000 description 7
- 210000001747 pupil Anatomy 0.000 description 7
- 238000010586 diagram Methods 0.000 description 6
- 238000006073 displacement reaction Methods 0.000 description 3
- 230000010287 polarization Effects 0.000 description 3
- 238000011156 evaluation Methods 0.000 description 2
- 238000003384 imaging method Methods 0.000 description 2
- 230000003321 amplification Effects 0.000 description 1
- 230000000903 blocking effect Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 230000004907 flux Effects 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 238000003199 nucleic acid amplification method Methods 0.000 description 1
- 230000004044 response Effects 0.000 description 1
- 230000001629 suppression Effects 0.000 description 1
Classifications
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- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/24—Base structure
- G02B21/241—Devices for focusing
- G02B21/245—Devices for focusing using auxiliary sources, detectors
-
- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/0004—Microscopes specially adapted for specific applications
- G02B21/0088—Inverse microscopes
-
- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B5/00—Optical elements other than lenses
- G02B5/005—Diaphragms
-
- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B7/00—Mountings, adjusting means, or light-tight connections, for optical elements
- G02B7/28—Systems for automatic generation of focusing signals
- G02B7/287—Systems for automatic generation of focusing signals including a sight line detecting device
-
- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/02—Objectives
-
- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/06—Means for illuminating specimens
- G02B21/08—Condensers
- G02B21/082—Condensers for incident illumination only
-
- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B27/00—Optical systems or apparatus not provided for by any of the groups G02B1/00 - G02B26/00, G02B30/00
- G02B27/10—Beam splitting or combining systems
- G02B27/14—Beam splitting or combining systems operating by reflection only
- G02B27/141—Beam splitting or combining systems operating by reflection only using dichroic mirrors
-
- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B27/00—Optical systems or apparatus not provided for by any of the groups G02B1/00 - G02B26/00, G02B30/00
- G02B27/28—Optical systems or apparatus not provided for by any of the groups G02B1/00 - G02B26/00, G02B30/00 for polarising
- G02B27/283—Optical systems or apparatus not provided for by any of the groups G02B1/00 - G02B26/00, G02B30/00 for polarising used for beam splitting or combining
Landscapes
- Physics & Mathematics (AREA)
- General Physics & Mathematics (AREA)
- Optics & Photonics (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Automatic Focus Adjustment (AREA)
- Microscoopes, Condenser (AREA)
Priority Applications (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2015188906A JP6590366B2 (ja) | 2015-09-25 | 2015-09-25 | 顕微鏡装置、オートフォーカス装置、及び、オートフォーカス方法 |
| US15/270,712 US10634895B2 (en) | 2015-09-25 | 2016-09-20 | Microscope apparatus, automatic focusing device, and automatic focusing method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2015188906A JP6590366B2 (ja) | 2015-09-25 | 2015-09-25 | 顕微鏡装置、オートフォーカス装置、及び、オートフォーカス方法 |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2017062438A JP2017062438A (ja) | 2017-03-30 |
| JP2017062438A5 JP2017062438A5 (enExample) | 2018-10-18 |
| JP6590366B2 true JP6590366B2 (ja) | 2019-10-16 |
Family
ID=58408959
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2015188906A Active JP6590366B2 (ja) | 2015-09-25 | 2015-09-25 | 顕微鏡装置、オートフォーカス装置、及び、オートフォーカス方法 |
Country Status (2)
| Country | Link |
|---|---|
| US (1) | US10634895B2 (enExample) |
| JP (1) | JP6590366B2 (enExample) |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP3514602A1 (de) | 2018-01-23 | 2019-07-24 | Universitatea Stefan cel Mare Suceava - Romania | Mikroskopobjektiv mit automatischer fokussierung |
| US11086118B2 (en) * | 2019-04-29 | 2021-08-10 | Molecular Devices, Llc | Self-calibrating and directional focusing systems and methods for infinity corrected microscopes |
| DE102019113975B4 (de) * | 2019-05-24 | 2023-10-19 | Abberior Instruments Gmbh | Verfahren und Vorrichtung zum Überwachen des Fokuszustands eines Mikroskops sowie Mikroskop |
Family Cites Families (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH07134242A (ja) | 1993-11-10 | 1995-05-23 | Olympus Optical Co Ltd | 焦点検出装置 |
| JPH08334317A (ja) | 1995-06-09 | 1996-12-17 | Olympus Optical Co Ltd | 測定顕微鏡 |
| JP5070696B2 (ja) | 2005-11-29 | 2012-11-14 | 株式会社ニコン | オートフォーカス装置とこれを有する顕微鏡 |
| JP5035600B2 (ja) | 2007-01-31 | 2012-09-26 | 横河電機株式会社 | 生物顕微鏡 |
| EP3067729B1 (en) * | 2007-09-03 | 2020-09-23 | Nikon Corporation | Automatic focusing device and microscope |
| US20120097835A1 (en) * | 2009-05-19 | 2012-04-26 | BioNano Geneomics, Inc. | Devices and methods for dynamic determination of sample position and orientation and dynamic repositioning |
| DE102011000835C5 (de) * | 2011-02-21 | 2019-08-22 | Leica Microsystems Cms Gmbh | Abtastmikroskop und Verfahren zur lichtmikroskopischen Abbildung eines Objektes |
| JP2012212018A (ja) | 2011-03-31 | 2012-11-01 | Nikon Corp | 焦点維持装置及び顕微鏡装置 |
| JP2013003333A (ja) * | 2011-06-16 | 2013-01-07 | Olympus Corp | 顕微鏡装置 |
| JP2016038408A (ja) * | 2014-08-05 | 2016-03-22 | オリンパス株式会社 | オートフォーカス装置、及び、標本観察装置 |
-
2015
- 2015-09-25 JP JP2015188906A patent/JP6590366B2/ja active Active
-
2016
- 2016-09-20 US US15/270,712 patent/US10634895B2/en active Active
Also Published As
| Publication number | Publication date |
|---|---|
| US10634895B2 (en) | 2020-04-28 |
| JP2017062438A (ja) | 2017-03-30 |
| US20170090175A1 (en) | 2017-03-30 |
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