JP6478444B2 - 改変された耐熱性ｄｎａポリメラーゼ - Google Patents

改変された耐熱性ｄｎａポリメラーゼ Download PDF

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Publication number
JP6478444B2
JP6478444B2 JP2013072515A JP2013072515A JP6478444B2 JP 6478444 B2 JP6478444 B2 JP 6478444B2 JP 2013072515 A JP2013072515 A JP 2013072515A JP 2013072515 A JP2013072515 A JP 2013072515A JP 6478444 B2 JP6478444 B2 JP 6478444B2
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Prior art keywords
dna polymerase
seq
thermococcus
modified
pcr
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Japanese (ja)
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JP2014079235A (ja
Inventor
哲大 小林
哲大 小林
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Toyobo Co Ltd
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Toyobo Co Ltd
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Priority to JP2013072515A priority Critical patent/JP6478444B2/ja
Priority to PCT/JP2013/076217 priority patent/WO2014051031A1/ja
Priority to US14/422,448 priority patent/US9963686B2/en
Priority to EP13841851.2A priority patent/EP2902488B1/en
Publication of JP2014079235A publication Critical patent/JP2014079235A/ja
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/07—Nucleotidyltransferases (2.7.7)
    • C12Y207/07007—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
JP2013072515A 2012-09-28 2013-03-29 改変された耐熱性ｄｎａポリメラーゼ Active JP6478444B2 (ja)

Priority Applications (4)

Application Number Priority Date Filing Date Title
JP2013072515A JP6478444B2 (ja) 2012-09-28 2013-03-29 改変された耐熱性ｄｎａポリメラーゼ
PCT/JP2013/076217 WO2014051031A1 (ja) 2012-09-28 2013-09-27 改変型ｄｎａポリメラーゼ
US14/422,448 US9963686B2 (en) 2012-09-28 2013-09-27 Modified DNA polymerase
EP13841851.2A EP2902488B1 (en) 2012-09-28 2013-09-27 Modified dna polymerase

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
JP2012217474 2012-09-28
JP2012217475 2012-09-28
JP2012217475 2012-09-28
JP2012217474 2012-09-28
JP2013072515A JP6478444B2 (ja) 2012-09-28 2013-03-29 改変された耐熱性ｄｎａポリメラーゼ

Publications (2)

Publication Number Publication Date
JP2014079235A JP2014079235A (ja) 2014-05-08
JP6478444B2 true JP6478444B2 (ja) 2019-03-06

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JP2013072515A Active JP6478444B2 (ja) 2012-09-28 2013-03-29 改変された耐熱性ｄｎａポリメラーゼ
JP2013072517A Active JP6478446B2 (ja) 2012-09-28 2013-03-29 改変された耐熱性ｄｎａポリメラーゼ
JP2013072516A Active JP6478445B2 (ja) 2012-09-28 2013-03-29 改変された耐熱性ｄｎａポリメラーゼ
JP2017093925A Active JP6536621B2 (ja) 2012-09-28 2017-05-10 改変された耐熱性ｄｎａポリメラーゼ
JP2017093924A Active JP6579152B2 (ja) 2012-09-28 2017-05-10 改変された耐熱性ｄｎａポリメラーゼ
JP2018000610A Active JP6579204B2 (ja) 2012-09-28 2018-01-05 改変された耐熱性ｄｎａポリメラーゼ
JP2019126133A Active JP6904382B2 (ja) 2012-09-28 2019-07-05 改変された耐熱性ｄｎａポリメラーゼ
JP2021038397A Active JP7044185B2 (ja) 2012-09-28 2021-03-10 改変された耐熱性ｄｎａポリメラーゼ

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JP2013072517A Active JP6478446B2 (ja) 2012-09-28 2013-03-29 改変された耐熱性ｄｎａポリメラーゼ
JP2013072516A Active JP6478445B2 (ja) 2012-09-28 2013-03-29 改変された耐熱性ｄｎａポリメラーゼ
JP2017093925A Active JP6536621B2 (ja) 2012-09-28 2017-05-10 改変された耐熱性ｄｎａポリメラーゼ
JP2017093924A Active JP6579152B2 (ja) 2012-09-28 2017-05-10 改変された耐熱性ｄｎａポリメラーゼ
JP2018000610A Active JP6579204B2 (ja) 2012-09-28 2018-01-05 改変された耐熱性ｄｎａポリメラーゼ
JP2019126133A Active JP6904382B2 (ja) 2012-09-28 2019-07-05 改変された耐熱性ｄｎａポリメラーゼ
JP2021038397A Active JP7044185B2 (ja) 2012-09-28 2021-03-10 改変された耐熱性ｄｎａポリメラーゼ

Country Status (4)

Country Link
US (1) US9963686B2 (enExample)
EP (1) EP2902488B1 (enExample)
JP (8) JP6478444B2 (enExample)
WO (1) WO2014051031A1 (enExample)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2015019951A1 (ja) * 2013-08-06 2015-02-12 東洋紡株式会社 核酸増幅法
JP6428997B2 (ja) * 2013-08-06 2018-11-28 東洋紡株式会社 核酸増幅法
JP2017093392A (ja) * 2015-11-27 2017-06-01 東洋紡株式会社 バイサルファイト処理された核酸増幅用ｄｎａポリメラーゼ
EP3402880B1 (en) 2016-01-15 2024-01-03 Thermo Fisher Scientific Baltics UAB Thermophilic dna polymerase mutants
US10273530B2 (en) 2016-01-15 2019-04-30 Thermo Fisher Scientific Baltics Uab Antibodies that bind thermophilic DNA polymerases
JP6933907B2 (ja) * 2017-02-24 2021-09-08 シスメックス株式会社 核酸増幅方法
EP3645710A1 (en) 2017-06-26 2020-05-06 Thermo Fisher Scientific Baltics Uab Thermophilic dna polymerase mutants
KR20210098844A (ko) * 2018-12-05 2021-08-11 일루미나, 인코포레이티드 중합효소, 조성물, 및 사용 방법
US11591629B2 (en) * 2019-12-23 2023-02-28 Cheng-Yao Chen Method and kit for template-independent nucleic acid synthesis

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DE69725076T2 (de) 1996-07-29 2004-04-15 Toyo Boseki K.K. Modifizierte thermostabile DNA Polymerase und eine DNA Polymerasezusammensetzung zur Amplifikation von Nukleinsäuren
JP3487394B2 (ja) * 1996-07-29 2004-01-19 東洋紡績株式会社 改変された耐熱性ｄｎａポリメラーゼおよびその用途
DE69806003T2 (de) * 1997-03-12 2003-01-30 Pe Corp. (Ny), Foster City Dna polymerasen, die verbesserte fähigkeiten zum einbau markierter nukleotide besitzen
ATE321867T1 (de) 1999-10-29 2006-04-15 Stratagene California Zusammensetzungen und methoden zur verwendung von dna polymerasen
EP1154017B1 (en) 2000-05-11 2010-01-20 Toyo Boseki Kabushiki Kaisha Modified thermostable dna polymerase from pyrococcus kodakarensis
JP3891330B2 (ja) * 2000-05-11 2007-03-14 東洋紡績株式会社 改変された耐熱性ｄｎａポリメラーゼ
DE60231901D1 (de) * 2001-11-14 2009-05-20 Toyo Boseki Dna-synthesepromotoren, mit dna-polymerase assoziierte faktoren und nutzung davon
GB0208768D0 (en) * 2002-04-17 2002-05-29 Univ Newcastle DNA polymerases
US20040081965A1 (en) 2002-10-25 2004-04-29 Stratagene DNA polymerases with reduced base analog detection activity
US8283148B2 (en) * 2002-10-25 2012-10-09 Agilent Technologies, Inc. DNA polymerase compositions for quantitative PCR and methods thereof
JP2006507012A (ja) * 2002-10-25 2006-03-02 ストラタジーン カリフォルニア 減少した塩基類似体検出活性を有するｄｎａポリメラーゼ
US7960157B2 (en) * 2002-12-20 2011-06-14 Agilent Technologies, Inc. DNA polymerase blends and uses thereof
DE602004025801D1 (de) 2003-03-25 2010-04-15 Stratagene California Dna-polymerasefusionen und deren verwendungen
GB0321306D0 (en) * 2003-09-11 2003-10-15 Solexa Ltd Modified polymerases for improved incorporation of nucleotide analogues
KR20090132645A (ko) * 2004-06-04 2009-12-30 다카라 바이오 가부시키가이샤 Ｄｎａ 폴리메라아제 활성을 갖는 폴리펩티드
KR100844358B1 (ko) * 2006-10-02 2008-07-07 한국해양연구원 돌연변이 ｄｎａ 중합효소들 및 그의 유전자들
JP2010035533A (ja) * 2008-08-08 2010-02-18 Canon Inc メチル化シトシン検出方法
EP2707481B1 (en) 2011-05-11 2016-07-13 New England Biolabs, Inc. Dna polymerase variants with reduced exonuclease activity and uses thereof
JP5461497B2 (ja) * 2011-09-28 2014-04-02 本田技研工業株式会社 油圧クラッチの初期制御値学習装置および車両
JP5727340B2 (ja) * 2011-09-28 2015-06-03 積水化学工業株式会社 たて管更新方法
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JP7007695B2 (ja) * 2016-07-04 2022-01-25 株式会社トキワ 塗布体付き化粧料容器

Also Published As

Publication number Publication date
EP2902488B1 (en) 2018-12-19
JP2014079236A (ja) 2014-05-08
JP2017131252A (ja) 2017-08-03
JP6536621B2 (ja) 2019-07-03
EP2902488A1 (en) 2015-08-05
JP2014079235A (ja) 2014-05-08
WO2014051031A1 (ja) 2014-04-03
JP6478446B2 (ja) 2019-03-06
US9963686B2 (en) 2018-05-08
JP2018046876A (ja) 2018-03-29
JP6579204B2 (ja) 2019-09-25
JP2019187444A (ja) 2019-10-31
JP7044185B2 (ja) 2022-03-30
US20150218534A1 (en) 2015-08-06
JP6579152B2 (ja) 2019-09-25
JP6478445B2 (ja) 2019-03-06
JP2014079237A (ja) 2014-05-08
EP2902488A4 (en) 2016-05-11
JP6904382B2 (ja) 2021-07-14
JP2017131253A (ja) 2017-08-03
JP2021097699A (ja) 2021-07-01

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