JP6437250B2 - 前処理方法及びそれに用いられる核酸抽出キット - Google Patents
前処理方法及びそれに用いられる核酸抽出キット Download PDFInfo
- Publication number
- JP6437250B2 JP6437250B2 JP2014179111A JP2014179111A JP6437250B2 JP 6437250 B2 JP6437250 B2 JP 6437250B2 JP 2014179111 A JP2014179111 A JP 2014179111A JP 2014179111 A JP2014179111 A JP 2014179111A JP 6437250 B2 JP6437250 B2 JP 6437250B2
- Authority
- JP
- Japan
- Prior art keywords
- nucleic acid
- silica particles
- acid amplification
- complex
- filter medium
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
- C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
- C12N15/1017—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by filtration, e.g. using filters, frits, membranes
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Biomedical Technology (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Analytical Chemistry (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Plant Pathology (AREA)
- Crystallography & Structural Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Immunology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Sampling And Sample Adjustment (AREA)
Priority Applications (14)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2014179111A JP6437250B2 (ja) | 2014-09-03 | 2014-09-03 | 前処理方法及びそれに用いられる核酸抽出キット |
| US15/505,329 US10316350B2 (en) | 2014-09-03 | 2015-09-02 | Pretreatment method and nucleic-acid-extracting kit usable for the same |
| ES15837848T ES2734068T3 (es) | 2014-09-03 | 2015-09-02 | Método de pretratamiento y kit de extracción de ácido nucleico utilizado en el citado método |
| PCT/JP2015/074927 WO2016035812A1 (ja) | 2014-09-03 | 2015-09-02 | 前処理方法及びそれに用いられる核酸抽出キット |
| KR1020177004470A KR101897177B1 (ko) | 2014-09-03 | 2015-09-02 | 전처리 방법 및 그것에 사용되는 핵산 추출 키트 |
| AU2015312884A AU2015312884B2 (en) | 2014-09-03 | 2015-09-02 | Pretreatment method and nucleic acid extraction kit used in said method |
| CA2957776A CA2957776C (en) | 2014-09-03 | 2015-09-02 | Pretreatment method and nucleic-acid-extracting kit usable for the same |
| MX2017002770A MX379186B (es) | 2014-09-03 | 2015-09-02 | Metodo de tratamiento previo y equipo de extraccion de acido nucleico utilizable para el mismo. |
| DK15837848.9T DK3190184T3 (da) | 2014-09-03 | 2015-09-02 | Forbehandlingsfremgangsmåde og nukleinsyreekstraktionskit, der anvendes i fremgangsmåden |
| MYPI2017700659A MY177446A (en) | 2014-09-03 | 2015-09-02 | Pretreatment method and nucleic acid extraction kit used in said method |
| PT15837848T PT3190184T (pt) | 2014-09-03 | 2015-09-02 | Método de pré-tratamento e kit de extração de ácido nucleico utilizado no referido método |
| EP15837848.9A EP3190184B1 (en) | 2014-09-03 | 2015-09-02 | Pretreatment method and nucleic acid extraction kit used in said method |
| BR112017004241A BR112017004241B8 (pt) | 2014-09-03 | 2015-09-02 | método de pré-tratamento e kit para extração de ácido nucléico usável para o mesmo |
| PH12017500258A PH12017500258B1 (en) | 2014-09-03 | 2017-02-10 | Pretreatment method and nucleic-acid-extracting kit usable for the same |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2014179111A JP6437250B2 (ja) | 2014-09-03 | 2014-09-03 | 前処理方法及びそれに用いられる核酸抽出キット |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2016052267A JP2016052267A (ja) | 2016-04-14 |
| JP2016052267A5 JP2016052267A5 (OSRAM) | 2017-09-28 |
| JP6437250B2 true JP6437250B2 (ja) | 2018-12-12 |
Family
ID=55439872
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2014179111A Active JP6437250B2 (ja) | 2014-09-03 | 2014-09-03 | 前処理方法及びそれに用いられる核酸抽出キット |
Country Status (14)
| Country | Link |
|---|---|
| US (1) | US10316350B2 (OSRAM) |
| EP (1) | EP3190184B1 (OSRAM) |
| JP (1) | JP6437250B2 (OSRAM) |
| KR (1) | KR101897177B1 (OSRAM) |
| AU (1) | AU2015312884B2 (OSRAM) |
| BR (1) | BR112017004241B8 (OSRAM) |
| CA (1) | CA2957776C (OSRAM) |
| DK (1) | DK3190184T3 (OSRAM) |
| ES (1) | ES2734068T3 (OSRAM) |
| MX (1) | MX379186B (OSRAM) |
| MY (1) | MY177446A (OSRAM) |
| PH (1) | PH12017500258B1 (OSRAM) |
| PT (1) | PT3190184T (OSRAM) |
| WO (1) | WO2016035812A1 (OSRAM) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP3733846A4 (en) * | 2017-12-27 | 2021-10-27 | Toray Industries, Inc. | METHOD FOR RECOVERY OF NUCLEIC ACID |
| JP7588815B2 (ja) | 2020-08-17 | 2024-11-25 | 株式会社ミズホメディー | Ｒｎａろ過器及びｒｎａろ過方法 |
Family Cites Families (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH01192494A (ja) * | 1988-01-27 | 1989-08-02 | Mitsubishi Electric Corp | レーザービームによる抜き金型の製造方法 |
| NL8900725A (nl) | 1989-03-23 | 1990-10-16 | Az Univ Amsterdam | Werkwijze en combinatie van middelen voor het isoleren van nucleinezuur. |
| GB9425138D0 (en) * | 1994-12-12 | 1995-02-08 | Dynal As | Isolation of nucleic acid |
| JPH10155481A (ja) | 1996-06-18 | 1998-06-16 | Rikagaku Kenkyusho | Ｄｎａの回収方法 |
| DE69719409T2 (de) | 1996-06-18 | 2004-01-15 | Rikagaku Kenkyusho | Verfahren zur Reinigung von DNS |
| JP4304348B2 (ja) | 1997-09-22 | 2009-07-29 | 独立行政法人理化学研究所 | Ｄｎａの単離方法 |
| JP3812696B2 (ja) * | 1997-11-17 | 2006-08-23 | 東洋紡績株式会社 | リボ核酸の抽出方法 |
| ATE374837T1 (de) * | 2002-01-08 | 2007-10-15 | Hoffmann La Roche | Verwendung eines silikamaterials bei der amplifikation |
| JP2004201607A (ja) | 2002-12-26 | 2004-07-22 | Asahi Kasei Corp | 核酸吸着固相体上でのｌａｍｐ反応 |
| EP1510577A1 (en) | 2003-08-29 | 2005-03-02 | Qiagen GmbH | Method for magnetic bead isolation of nucleic acids |
| JP4831725B2 (ja) * | 2004-02-13 | 2011-12-07 | 栄研化学株式会社 | 簡易的核酸抽出法 |
| US20060257907A1 (en) | 2005-04-19 | 2006-11-16 | The Regents Of The University Of California | Packed bed for nucleic acid capture and amplification |
| US20110091873A1 (en) | 2009-10-21 | 2011-04-21 | Microfluidic Systems, Inc. | Integrated sample preparation and amplification for nucleic acid detection from biological samples |
| JP6024266B2 (ja) * | 2012-08-01 | 2016-11-16 | セイコーエプソン株式会社 | Ｄｎａの抽出方法 |
-
2014
- 2014-09-03 JP JP2014179111A patent/JP6437250B2/ja active Active
-
2015
- 2015-09-02 ES ES15837848T patent/ES2734068T3/es active Active
- 2015-09-02 MY MYPI2017700659A patent/MY177446A/en unknown
- 2015-09-02 KR KR1020177004470A patent/KR101897177B1/ko active Active
- 2015-09-02 BR BR112017004241A patent/BR112017004241B8/pt active IP Right Grant
- 2015-09-02 PT PT15837848T patent/PT3190184T/pt unknown
- 2015-09-02 MX MX2017002770A patent/MX379186B/es unknown
- 2015-09-02 AU AU2015312884A patent/AU2015312884B2/en active Active
- 2015-09-02 DK DK15837848.9T patent/DK3190184T3/da active
- 2015-09-02 CA CA2957776A patent/CA2957776C/en active Active
- 2015-09-02 EP EP15837848.9A patent/EP3190184B1/en active Active
- 2015-09-02 US US15/505,329 patent/US10316350B2/en active Active
- 2015-09-02 WO PCT/JP2015/074927 patent/WO2016035812A1/ja not_active Ceased
-
2017
- 2017-02-10 PH PH12017500258A patent/PH12017500258B1/en unknown
Also Published As
| Publication number | Publication date |
|---|---|
| WO2016035812A1 (ja) | 2016-03-10 |
| KR20170028443A (ko) | 2017-03-13 |
| ES2734068T3 (es) | 2019-12-04 |
| BR112017004241A2 (pt) | 2017-12-12 |
| CA2957776C (en) | 2021-01-05 |
| MY177446A (en) | 2020-09-15 |
| EP3190184A1 (en) | 2017-07-12 |
| US20170335370A1 (en) | 2017-11-23 |
| AU2015312884A1 (en) | 2017-03-02 |
| BR112017004241B8 (pt) | 2021-07-27 |
| PH12017500258A1 (en) | 2017-07-03 |
| PT3190184T (pt) | 2019-06-27 |
| BR112017004241B1 (pt) | 2020-12-01 |
| EP3190184B1 (en) | 2019-04-10 |
| CA2957776A1 (en) | 2016-03-10 |
| PH12017500258B1 (en) | 2017-07-03 |
| EP3190184A4 (en) | 2018-02-14 |
| AU2015312884B2 (en) | 2018-06-28 |
| KR101897177B1 (ko) | 2018-09-10 |
| MX379186B (es) | 2025-03-10 |
| MX2017002770A (es) | 2017-05-30 |
| US10316350B2 (en) | 2019-06-11 |
| DK3190184T3 (da) | 2019-06-24 |
| JP2016052267A (ja) | 2016-04-14 |
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