JP6420297B2 - 流体中の物体の分析および選別 - Google Patents
流体中の物体の分析および選別 Download PDFInfo
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Description
本発明の実施形態の目的は、流れる媒体中の物体の高速で効率的な選別を提供することである。
・前記流体の流れを複数のマイクロ流体チャネルに導入するステップ。マイクロ流体チャネルは、撮像領域を含む。
・前記撮像領域のいずれかを通過する際、物体のホログラフィック回折像を記録するステップ。
・前記撮像領域のいずれかを通過する際、前記物体の各々について得られるホログラフィック回折像をリアルタイムで特性評価を行うステップ。前記特性評価は、少なくとも1つの予め定めた物体タイプの痕跡を考慮する。
・各物体を出口に誘導(steer)するステップ。前記出口は、前記物体の前記特性評価の関数として複数の出口から選択される。
一滴の血液は、酸素を運ぶ赤血球、感染と戦って免疫システムの基本要素を形成する白血球(leukocytes)およびその亜型、血小板、血漿の複雑な混合物を含む。各細胞集団の総数を計数する血液計数は、多くの形態の病気の存在を示すために使用でき、従って、医療分野で最も一般的に実施される血液検査の1つである。看護師がサンプルを収集し、凝固を停止する抗凝固剤(EDTA,時にはクエン酸)を含む検査チューブに血液を吸引する。そして、サンプルは研究室に搬送される。FACS、フィコール勾配、磁気ビーズベースの分離、手動計数などの方法が、典型的には研究室において使用され、種々の亜型の血液細胞を計数または分離している。
・バイオリアクタを連続的にサンプリングするバイパス流での連続的監視。光学システムがバイオリアクタの外部に置くことができる。
・バイオリアクタ壁の一部であるマイクロ流体消耗品での連続的監視。例えば、バイオリアクタ自体が、消耗品材料で製作されることを意味する場合。
・既存のインライン方法に類似したバイオリアクタ内部での連続的監視。
・いくつかの場所でバイオリアクタ内部での連続的監視。理由として、ホログラフィック撮像器がかなりコンパクトであり、多重監視が大型リアクタ容器内で使用できる。その結果、より完全な測定が行われ、結果が、混合などのプロセスパラメータにあまり依存しない。
・マイクロ流体バイオリアクタの場合、撮像システムは、バイオリアクタの流体ハンドリングシステムの一部として実装できる。
・マイクロ流体システム内に形成できる結晶、例えば、タンパク質結晶の選別。
・ナノ粒子、例えば、多分散系の粒子サンプルの選別。
・ナノ粒子分析試料の一部であるナノ粒子の選別。
Claims (4)
- 流れる媒体中に浸漬された物体を選別するためのデバイス(1)であって、
流れる媒体を照射するための少なくとも部分的にコヒーレントな光源(8)、該光源(8)からの光が通過するピンホール(32,42,64)、および複数のホログラフィック回折像を提供するための複数の撮像素子(2,37,45,202)を含むホログラフィック撮像ユニットと、
複数のマイクロ流体チャネル(3)を含む流体ハンドリングユニットとを備え、
マイクロ流体チャネル(3)は、流れる媒体中に浸漬された移動物体を撮像するために、対応する撮像素子(2,37,45,202)に沿って流れる媒体を案内するための撮像領域(4)を含み、
マイクロ流体チャネル(3)は、複数の出口(6)のうち選択した1つに、流れる媒体中の各物体を制御可能に方向付けるために、撮像領域の下流に配置されたマイクロ流体スイッチ(5)をさらに含み、
デバイス(1)は、撮像領域(4)のいずれかを通過する際、物体の各々について得られるホログラフィック回折像のリアルタイム特性評価に適合した処理ユニット(7)をさらに備え、
該特性評価は、少なくとも1つの所定の物体タイプの痕跡を考慮するものであり、
該処理ユニット(7)は、該特性評価に応答して、撮像領域(4)の下流にあるマイクロ流体スイッチ(5)を制御するように構成され、
ホログラフィック撮像ユニットは、複数の有効画素アレイに分割されたCMOSまたはCCDのイメージセンサを含み、
各有効画素アレイは、それぞれのマイクロ流体チャネル(3)の撮像領域(4)に関連しており、
物体と撮像素子(2,37,45,202)との間の距離Z1、および物体とピンホール(32,42,64)との間の距離Z2は、Z1>Z2を満たす、デバイス(1)。 - イメージセンサは、複数のホログラフィック回折像を同時にかつ互いに独立して出力するように、複数の有効画素アレイからの信号を並列的に読み出すように構成された、請求項1記載のデバイス(1)。
- ピンホール(32,42,64)は、物体から40μm〜400μmの範囲に設置され、撮像素子(2,37,45,202)から1mm〜10mmの範囲に設置される、請求項1記載のデバイス(1)。
- 複数のマイクロ流体チャネルの各々が、撮像領域(4)とマイクロ流体スイッチ(5)との間に配置され、リアルタイム特性評価を実施しながら、撮像領域(4)からマイクロ流体スイッチ(5)への物体の各々の転移を遅延させるための蛇行セグメントをさらに備える、請求項1〜3のいずれかに記載のデバイス(1)。
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US20170061619A1 (en) | 2017-03-02 |
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US9495742B2 (en) | 2016-11-15 |
CN104136907B (zh) | 2017-03-08 |
US20140376816A1 (en) | 2014-12-25 |
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