SG11202011180UA - Automated detection and characterization of micro-objects in microfluidic devices - Google Patents

Automated detection and characterization of micro-objects in microfluidic devices

Info

Publication number
SG11202011180UA
SG11202011180UA SG11202011180UA SG11202011180UA SG11202011180UA SG 11202011180U A SG11202011180U A SG 11202011180UA SG 11202011180U A SG11202011180U A SG 11202011180UA SG 11202011180U A SG11202011180U A SG 11202011180UA SG 11202011180U A SG11202011180U A SG 11202011180UA
Authority
SG
Singapore
Prior art keywords
characterization
micro
objects
microfluidic devices
automated detection
Prior art date
Application number
SG11202011180UA
Inventor
John A Tenney
Thomas M Vetterli
Hansohl E Kim
Original Assignee
Berkeley Lights Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Berkeley Lights Inc filed Critical Berkeley Lights Inc
Publication of SG11202011180UA publication Critical patent/SG11202011180UA/en

Links

Classifications

    • G—PHYSICS
    • G06—COMPUTING; CALCULATING OR COUNTING
    • G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T7/00—Image analysis
    • G06T7/10—Segmentation; Edge detection
    • G06T7/11—Region-based segmentation
    • G—PHYSICS
    • G06—COMPUTING; CALCULATING OR COUNTING
    • G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T7/00—Image analysis
    • G06T7/0002—Inspection of images, e.g. flaw detection
    • G06T7/0012—Biomedical image inspection
    • G—PHYSICS
    • G06—COMPUTING; CALCULATING OR COUNTING
    • G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T7/00—Image analysis
    • G06T7/10—Segmentation; Edge detection
    • G06T7/174—Segmentation; Edge detection involving the use of two or more images
    • G—PHYSICS
    • G06—COMPUTING; CALCULATING OR COUNTING
    • G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T7/00—Image analysis
    • G06T7/30—Determination of transform parameters for the alignment of images, i.e. image registration
    • G—PHYSICS
    • G06—COMPUTING; CALCULATING OR COUNTING
    • G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T2207/00—Indexing scheme for image analysis or image enhancement
    • G06T2207/10—Image acquisition modality
    • G06T2207/10056—Microscopic image
    • G—PHYSICS
    • G06—COMPUTING; CALCULATING OR COUNTING
    • G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T2207/00—Indexing scheme for image analysis or image enhancement
    • G06T2207/10—Image acquisition modality
    • G06T2207/10064—Fluorescence image
    • G—PHYSICS
    • G06—COMPUTING; CALCULATING OR COUNTING
    • G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T2207/00—Indexing scheme for image analysis or image enhancement
    • G06T2207/10—Image acquisition modality
    • G06T2207/10141—Special mode during image acquisition
    • G06T2207/10152—Varying illumination
    • G—PHYSICS
    • G06—COMPUTING; CALCULATING OR COUNTING
    • G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T2207/00—Indexing scheme for image analysis or image enhancement
    • G06T2207/20—Special algorithmic details
    • G06T2207/20081—Training; Learning
    • G—PHYSICS
    • G06—COMPUTING; CALCULATING OR COUNTING
    • G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T2207/00—Indexing scheme for image analysis or image enhancement
    • G06T2207/20—Special algorithmic details
    • G06T2207/20084—Artificial neural networks [ANN]
    • G—PHYSICS
    • G06—COMPUTING; CALCULATING OR COUNTING
    • G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T2207/00—Indexing scheme for image analysis or image enhancement
    • G06T2207/20—Special algorithmic details
    • G06T2207/20212—Image combination
    • G06T2207/20224—Image subtraction
    • G—PHYSICS
    • G06—COMPUTING; CALCULATING OR COUNTING
    • G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T2207/00—Indexing scheme for image analysis or image enhancement
    • G06T2207/30—Subject of image; Context of image processing
    • G06T2207/30004—Biomedical image processing
    • G06T2207/30024—Cell structures in vitro; Tissue sections in vitro
    • G—PHYSICS
    • G06—COMPUTING; CALCULATING OR COUNTING
    • G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T2207/00—Indexing scheme for image analysis or image enhancement
    • G06T2207/30—Subject of image; Context of image processing
    • G06T2207/30242—Counting objects in image

Landscapes

  • Engineering & Computer Science (AREA)
  • Computer Vision & Pattern Recognition (AREA)
  • Physics & Mathematics (AREA)
  • General Physics & Mathematics (AREA)
  • Theoretical Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Medical Informatics (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Radiology & Medical Imaging (AREA)
  • Quality & Reliability (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Physical Or Chemical Processes And Apparatus (AREA)
SG11202011180UA 2018-05-31 2019-05-31 Automated detection and characterization of micro-objects in microfluidic devices SG11202011180UA (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201862678791P 2018-05-31 2018-05-31
PCT/US2019/035046 WO2019232473A2 (en) 2018-05-31 2019-05-31 Automated detection and characterization of micro-objects in microfluidic devices

Publications (1)

Publication Number Publication Date
SG11202011180UA true SG11202011180UA (en) 2020-12-30

Family

ID=68698993

Family Applications (1)

Application Number Title Priority Date Filing Date
SG11202011180UA SG11202011180UA (en) 2018-05-31 2019-05-31 Automated detection and characterization of micro-objects in microfluidic devices

Country Status (10)

Country Link
US (1) US20210209752A1 (en)
EP (1) EP3803341A4 (en)
JP (1) JP2021525359A (en)
KR (1) KR20210015947A (en)
CN (1) CN112204380A (en)
AU (1) AU2019276567A1 (en)
CA (1) CA3100701A1 (en)
IL (1) IL279058A (en)
SG (1) SG11202011180UA (en)
WO (1) WO2019232473A2 (en)

Families Citing this family (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US10675625B2 (en) 2016-04-15 2020-06-09 Berkeley Lights, Inc Light sequencing and patterns for dielectrophoretic transport
IL267008B2 (en) 2016-12-01 2024-06-01 Berkeley Lights Inc Apparatuses, Systems and Methods for Imaging Micro-Objects
EP3549099A4 (en) 2016-12-01 2020-07-29 Berkeley Lights, Inc. Automated detection and repositioning of micro-objects in microfluidic devices
JP7356351B2 (en) 2016-12-12 2023-10-04 エクセラ・バイオサイエンシーズ・インコーポレイテッド Methods and systems for screening using microcapillary arrays
WO2019161562A1 (en) * 2018-02-26 2019-08-29 Intel Corporation Object detection with image background subtracted
WO2020021603A1 (en) * 2018-07-23 2020-01-30 株式会社島津製作所 Microfluidic device observation device
KR20220097404A (en) 2019-10-10 2022-07-07 1859, 인크. Methods and systems for microfluidic screening
US11335636B2 (en) * 2019-10-29 2022-05-17 Hefei Reliance Memory Limited Gradual breakdown memory cell having multiple different dielectrics
IL292993A (en) * 2019-11-17 2022-07-01 Berkeley Lights Inc Systems and methods for analyses of biological samples
JP2023519489A (en) 2020-02-21 2023-05-11 サージビジョン・ゲゼルシャフト・ミット・ベシュレンクテル・ハフツング Supporting medical procedures using luminescence images processed with restricted information regions identified in corresponding auxiliary images
US11602132B2 (en) 2020-10-06 2023-03-14 Sixgill, LLC System and method of counting livestock
CN112907665B (en) * 2021-02-07 2022-06-10 吉林大学 Micro-fluidic blood type detection card micro-cavity reaction tank precise positioning method based on RGB color space
CN114241245B (en) * 2021-12-23 2024-05-31 西南大学 Image classification system based on residual capsule neural network
CN114627369B (en) * 2022-01-19 2024-03-22 上海土蜂科技有限公司 Environment monitoring system, method and computer device thereof
WO2024095445A1 (en) * 2022-11-04 2024-05-10 日本電気株式会社 Information processing system, information processing device, information processing method, and storage medium
WO2024111263A1 (en) * 2022-11-24 2024-05-30 ソニーグループ株式会社 Biological sample analysis device, biological sample analysis system, and method for verifying status of biological sample analysis device
CN116758070B (en) * 2023-08-17 2023-12-12 深圳安侣医学科技有限公司 Method and system for 3-dimensional analysis of cells and obtaining 3-dimensional morphological characteristics of cells

Family Cites Families (34)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6294063B1 (en) 1999-02-12 2001-09-25 Board Of Regents, The University Of Texas System Method and apparatus for programmable fluidic processing
US6942776B2 (en) 1999-05-18 2005-09-13 Silicon Biosystems S.R.L. Method and apparatus for the manipulation of particles by means of dielectrophoresis
DK1065378T3 (en) 1999-06-28 2002-07-29 California Inst Of Techn Elastomeric micropump and micro valve systems
JP2002335400A (en) * 2001-05-07 2002-11-22 Sharp Corp Image processing unit, image processing method and recording medium
US6958132B2 (en) 2002-05-31 2005-10-25 The Regents Of The University Of California Systems and methods for optical actuation of microfluidics based on opto-electrowetting
WO2005100541A2 (en) 2004-04-12 2005-10-27 The Regents Of The University Of California Optoelectronic tweezers for microparticle and cell manipulation
US8685344B2 (en) 2007-01-22 2014-04-01 Advanced Liquid Logic, Inc. Surface assisted fluid loading and droplet dispensing
WO2008119066A1 (en) 2007-03-28 2008-10-02 The Regents Of The University Of California Single-sided lateral-field and phototransistor-based optoelectronic tweezers
EP2158574B1 (en) * 2007-06-21 2018-12-12 Koninklijke Philips N.V. Model-based differential diagnosis of dementia and interactive setting of level of significance
ITTO20070771A1 (en) * 2007-10-29 2009-04-30 Silicon Biosystems Spa METHOD AND APPARATUS FOR IDENTIFICATION AND HANDLING OF PARTICLES
JP5525454B2 (en) * 2008-01-28 2014-06-18 フラム・グループ・アイピー・エルエルシー High position thread ground shield
FR2935802B1 (en) * 2008-09-05 2012-12-28 Horiba Abx Sas METHOD AND DEVICE FOR CLASSIFYING, VISUALIZING AND EXPLORING BIOLOGICAL DATA
US9025850B2 (en) * 2010-06-25 2015-05-05 Cireca Theranostics, Llc Method for analyzing biological specimens by spectral imaging
US9533306B2 (en) 2010-08-02 2017-01-03 The Regents Of The University Of California Single sided continuous optoelectrowetting (SCEOW) device for droplet manipulation with light patterns
US20120225475A1 (en) * 2010-11-16 2012-09-06 1087 Systems, Inc. Cytometry system with quantum cascade laser source, acoustic detector, and micro-fluidic cell handling system configured for inspection of individual cells
EP2683810A4 (en) * 2011-03-07 2014-12-31 Univ Toronto Method and system for cell detection and analysis
US9227200B2 (en) 2011-06-03 2016-01-05 The Regents Of The University Of California Microfluidic devices with flexible optically transparent electrodes
EP2602608B1 (en) * 2011-12-07 2016-09-14 Imec Analysis and sorting of biological cells in flow
JP6366580B2 (en) * 2012-06-22 2018-08-01 エイチティージー モレキュラー ダイアグノスティクス， インコーポレイテッド Molecular malignancy in melanocytic lesions
US9857333B2 (en) 2012-10-31 2018-01-02 Berkeley Lights, Inc. Pens for biological micro-objects
US9403172B2 (en) 2012-11-08 2016-08-02 Berkeley Lights, Inc. Circuit based optoelectronic tweezers
US9889445B2 (en) 2013-10-22 2018-02-13 Berkeley Lights, Inc. Micro-fluidic devices for assaying biological activity
EP3760703A1 (en) * 2013-10-22 2021-01-06 Berkeley Lights, Inc. Microfluidic devices having isolation pens and methods of testing biological micro-objects with same
US20150306598A1 (en) 2014-04-25 2015-10-29 Berkeley Lights, Inc. DEP Force Control And Electrowetting Control In Different Sections Of The Same Microfluidic Apparatus
US20150306599A1 (en) 2014-04-25 2015-10-29 Berkeley Lights, Inc. Providing DEP Manipulation Devices And Controllable Electrowetting Devices In The Same Microfluidic Apparatus
EP3134739B1 (en) 2014-04-25 2019-07-10 Berkeley Lights, Inc. Providing dep manipulation devices and controllable electrowetting devices in the same microfluidic apparatus
CN107206377B (en) * 2014-12-09 2020-02-11 伯克利之光生命科技公司 Automated detection of positive assay regions in microfluidic devices
EP3230717B1 (en) * 2014-12-09 2021-08-18 Berkeley Lights, Inc. Automated detection and identification of micro-objects in microfluidic devices
AU2016222894A1 (en) * 2015-02-23 2017-08-31 Cellanyx Diagnostics, Llc Cell imaging and analysis to differentiate clinically relevant sub-populations of cells
WO2016172623A1 (en) * 2015-04-22 2016-10-27 Berkeley Lights, Inc. Manipulation of cell nuclei in a micro-fluidic device
TWI756199B (en) * 2016-01-15 2022-03-01 美商伯克利之光生命科技公司 Methods of producing patient-specific anti-cancer therapeutics and methods of treatment therefor
EP3922716A1 (en) * 2016-03-17 2021-12-15 Berkeley Lights, Inc. Selection and cloning of t lymphocytes in a microfluidic device
US20180135011A1 (en) * 2017-03-13 2018-05-17 Berkeley Lights, Inc. Selection and cloning of t lymphocytes in a microfluidic device
CN115639134A (en) * 2016-06-10 2023-01-24 加利福尼亚大学董事会 Image-based cell sorting system and method

Also Published As

Publication number Publication date
US20210209752A1 (en) 2021-07-08
IL279058A (en) 2021-01-31
CA3100701A1 (en) 2019-12-05
KR20210015947A (en) 2021-02-10
AU2019276567A1 (en) 2021-01-28
EP3803341A2 (en) 2021-04-14
EP3803341A4 (en) 2022-03-23
JP2021525359A (en) 2021-09-24
WO2019232473A3 (en) 2020-01-16
WO2019232473A2 (en) 2019-12-05
CN112204380A (en) 2021-01-08

Similar Documents

Publication Publication Date Title
IL279058A (en) Automated detection and characterization of micro-objects in microfluidic devices
IL267009A (en) Automated detection and repositioning of micro-objects in microfluidic devices
HK1244874A1 (en) Automated detection and repositioning of micro-objects in microfluidic devices
HK1244465A1 (en) Automated detection of assay-positive areas in microfluidic devices
HK1251660A1 (en) Microfluidic droplet detection and sorting
HK1251658A1 (en) Droplet microfluidic device and methods of sensing the result of an assay therein
SG11201914032VA (en) Single-channel chemiluminescent micro-fluidic chip and detection method thereof
EP3548889A4 (en) Automated point-of-care devices for complex sample processing and methods of use thereof
HK1251659A1 (en) Droplet microfluidic device and methods of sensing the result of an assay therein
SG11201607270PA (en) A multi-index detection microfluidic chip and methods of use
SG11201609288YA (en) Fully-automatic immunofluorescence quantitative analysis device and detection method
HK1258244A1 (en) Fluidic analysis and separation
EP3544509A4 (en) Automated detection and identification of phantoms
EP3252476A4 (en) Liquid surface inspection device, automated analysis device, and processing device
EP3485973A4 (en) Microfluidic reagent card and detection method and application thereof
SG10201912318VA (en) Analyte detection methods and apparatus using dielectrophoresis and electroosmosis
SG11201800647SA (en) Biological condition determination device and biological condition determination method
EP3410107A4 (en) Analysis device and separation device
EP3528030A4 (en) Sample observation device and sample observation method
IL252403A0 (en) Amplification and detection of nucleic acids in a biological sample
EP3275549A4 (en) Microfluidic device and sample analysis device comprising same
HK1247987A1 (en) Fluidic bridge device and sample processing methods
EP3644046A4 (en) Specimen detection device and specimen detection method
SG11202005577PA (en) Automated liquid handling and testing systems and methods
IL266270A (en) Protein screening and detection method