JP6407248B2 - 細胞外核酸の安定化および単離 - Google Patents

細胞外核酸の安定化および単離 Download PDF

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Publication number
JP6407248B2
JP6407248B2 JP2016503570A JP2016503570A JP6407248B2 JP 6407248 B2 JP6407248 B2 JP 6407248B2 JP 2016503570 A JP2016503570 A JP 2016503570A JP 2016503570 A JP2016503570 A JP 2016503570A JP 6407248 B2 JP6407248 B2 JP 6407248B2
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sample
cell
nucleic acid
stabilization
oxyethylene
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JP2016520293A5 (enExample
JP2016520293A (ja
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ラルフ ヴュリヒ,
ラルフ ヴュリヒ,
トルステン フォス,
トルステン フォス,
ダニエル グレルツ,
ダニエル グレルツ,
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Qiagen GmbH
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
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  • Genetics & Genomics (AREA)
  • Analytical Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • General Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biomedical Technology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Sampling And Sample Adjustment (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Peptides Or Proteins (AREA)
JP2016503570A 2013-03-18 2014-03-18 細胞外核酸の安定化および単離 Active JP6407248B2 (ja)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
EP13159834 2013-03-18
EP13159834.4 2013-03-18
EP13180086 2013-08-12
EP13180086.4 2013-08-12
PCT/EP2014/000724 WO2014146780A1 (en) 2013-03-18 2014-03-18 Stabilization and isolation of extracellular nucleic acids

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JP2016520293A JP2016520293A (ja) 2016-07-14
JP2016520293A5 JP2016520293A5 (enExample) 2017-02-16
JP6407248B2 true JP6407248B2 (ja) 2018-10-17

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JP2016503570A Active JP6407248B2 (ja) 2013-03-18 2014-03-18 細胞外核酸の安定化および単離
JP2016503572A Pending JP2016518827A (ja) 2013-03-18 2014-03-18 細胞外核酸の安定化および単離

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EP (2) EP2976425B1 (enExample)
JP (2) JP6407248B2 (enExample)
CN (2) CN105164258B (enExample)
WO (2) WO2014146782A1 (enExample)

Families Citing this family (25)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US11021733B2 (en) 2011-09-26 2021-06-01 Qiagen Gmbh Stabilization and isolation of extracellular nucleic acids
CN103827303B (zh) 2011-09-26 2021-10-22 普瑞阿那利提克斯有限公司 细胞外核酸的稳定化和分离
ES2971646T3 (es) 2011-09-26 2024-06-06 Preanalytix Gmbh Estabilización y aislamiento de ácidos nucleicos extracelulares
US20150225712A1 (en) * 2012-08-21 2015-08-13 Qiagen Gmbh Method for isolating nucleic acids from a formaldehyde releaser stabilized sample
CN104755628B (zh) 2012-09-25 2019-03-01 凯杰有限公司 生物样品的稳定化
CA2886389A1 (en) 2012-09-28 2014-04-03 Cepheid Methods for dna and rna extraction from fixed paraffin-embedded tissue samples
US11525155B2 (en) 2013-03-18 2022-12-13 Qiagen Gmbh Stabilisation of biological samples
US20160281133A1 (en) 2013-03-18 2016-09-29 Qiagen Gmbh Stabilization and isolation of extracellular nucleic acids
JP6664332B2 (ja) 2014-03-18 2020-03-13 キアゲン ゲーエムベーハー 細胞外核酸の安定化および単離
AU2016298048B2 (en) * 2015-07-24 2022-08-04 Cepheid Compositions and methods for DNA and RNA extraction from tissue samples
EP3377645B1 (en) 2015-11-20 2023-10-04 Qiagen GmbH Method of preparing sterilized compositions for stabilization of extracellular nucleic acids
CN109311930B (zh) 2016-03-31 2023-02-24 伯克利之光生命科技公司 核酸稳定试剂、试剂盒及其使用方法
CN109153992B (zh) 2016-05-27 2022-11-29 诺根生物科技公司 生物样本中游离核酸的保存
CN109890977A (zh) * 2016-08-17 2019-06-14 加利福尼亚大学董事会 通过基于生物流体的无细胞dna(cfdna)测定评价器官损伤状态的基于免疫探针的新方法
EP3358020B1 (en) 2017-01-05 2021-05-19 Biodesix, Inc. Diagnostic test system for specific, sensitive and reproducible detection of circulating nucleic acids in whole blood
CN110997933B (zh) * 2017-08-02 2024-01-09 萨斯特德特股份有限两合公司 用于稳定无细胞核酸和细胞的方法和组合物
JP7548904B2 (ja) * 2019-01-04 2024-09-10 キアゲン ゲーエムベーハー 尿安定化
US20220349014A1 (en) 2019-09-24 2022-11-03 Qiagen Gmbh Multimodal analysis of stabilized cell-containing bodily fluid samples
US20230203586A1 (en) * 2020-05-29 2023-06-29 The Rockefeller University Method and system for rna isolation from self-collected and small volume samples
JP7635480B2 (ja) * 2020-11-27 2025-02-26 大王製紙株式会社 Rfidタグ、採血管、及びアンテナ
EP4263383A4 (en) 2020-12-17 2024-10-23 Nephrosant, Inc. KITS FOR STABILIZATION OF URINE SAMPLES
US20250235405A1 (en) 2021-11-19 2025-07-24 Branca Bunus Limited A composition comprising a therapeutically active agent packaged within a drug delivery vehicle
JP7586593B2 (ja) * 2022-09-29 2024-11-19 積水メディカル株式会社 血液保存用組成物及び血液採取容器
EP4356731A1 (en) 2022-10-19 2024-04-24 Olga Alekseevna Gra Medium and device for collecting, storing and transporting biological samples, as well as their use for the stabilization of extracellular nucleic acids
EP4477751A1 (en) 2023-06-16 2024-12-18 QIAGEN GmbH Body fluid stabilization and processing

Family Cites Families (94)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3389133A (en) * 1966-08-10 1968-06-18 Schwarz Biores Inc Process for isolating and purifying soluble ribonucleic acid
US3903179A (en) 1973-10-15 1975-09-02 Gulf Research Development Co Method for preparing nitrocyclopropane from 3-chloro-1-nitropropane
JPS6083581A (ja) * 1983-10-13 1985-05-11 Hideaki Yamada シユ−ドモナス属細菌の培養法
US4938961A (en) 1989-04-28 1990-07-03 Geoffrey Collins Organ preservation solution containing pokyethylene gycol and method of performing cardioplegia
US5459073A (en) 1991-05-08 1995-10-17 Streck Laboratories, Inc. Method and composition for preserving antigens and process for utilizing cytological material produced by same
US5460797A (en) 1991-05-08 1995-10-24 Streck Laboratories, Inc. Method for fixing tissues and cells for analysis using oxazolidine compounds
GB9215002D0 (en) * 1992-07-15 1992-08-26 Univ Singapore Method of fixing biological tissue
WO1995021849A1 (de) 1994-02-11 1995-08-17 Qiagen Gmbh Verfahren zur trennung von doppelstrang/einzelstrangnukleinsäurestrukturen
US5705628A (en) 1994-09-20 1998-01-06 Whitehead Institute For Biomedical Research DNA purification and isolation using magnetic particles
WO1996021849A2 (en) 1994-12-29 1996-07-18 Brookfield Engineering Laboratories, Inc. Support system with radially rigid wire suspension
US6673364B1 (en) 1995-06-07 2004-01-06 The University Of British Columbia Liposome having an exchangeable component
US20050158699A1 (en) 1995-06-07 2005-07-21 Kadkade Prakash G. Cryopreservation of plant cells
JPH11513255A (ja) * 1995-10-06 1999-11-16 イー・アイ・デユポン・ドウ・ヌムール・アンド・カンパニー 立体特異的ニトリルヒドラターゼおよびアミダーゼ酵素をコードする核酸断片、ならびにキラルアミドおよび酸の生産に有用なそれら酵素を発現する組換え微生物
ATE284891T1 (de) 1996-02-14 2005-01-15 Biomerieux Bv Isolierung von einzelsträngigen nukleinsäuren
EP0929694A4 (en) 1996-03-15 2002-05-02 Penn State Res Found DETECTION OF TUMOR-RELATED EXTRACELLULAR NUCLEIC ACID USING PLASMA OR BLOOD SERUM USING NUCLEIC ACID AMPLIFICATION TESTS
PT938320E (pt) 1996-03-26 2010-09-22 Michael S Kopreski Método que permite a utilização de arn extracelular extraído de plasma ou de soro para detectar, monitorizar ou avaliar o cancro
AU5532498A (en) 1996-12-27 1998-07-31 Sherwood Medical Company Anticoagulant composition
WO1998041651A1 (en) 1997-03-18 1998-09-24 Hsc Research & Development Limited Partnership Method for preparing chromatin
DE19726362A1 (de) 1997-06-21 1998-12-24 Schaeffler Waelzlager Ohg Vorrichtung zum Verändern der Steuerzeiten von Gaswechselventilen einer Brennkraftmaschine
US6723564B2 (en) * 1998-05-07 2004-04-20 Sequenom, Inc. IR MALDI mass spectrometry of nucleic acids using liquid matrices
WO1999058664A1 (en) 1998-05-14 1999-11-18 Whitehead Institute For Biomedical Research Solid phase technique for selectively isolating nucleic acids
DE19836559A1 (de) 1998-08-12 2000-03-23 Antigen Gmbh Gefäß zur Entnahme von Blut
JP2000187033A (ja) * 1998-12-21 2000-07-04 Wako Pure Chem Ind Ltd 細胞染色液
US6407107B1 (en) * 1999-05-28 2002-06-18 The Board Of Trustees Of The Unversity Of Arkansas Derivatives of butyric acid and uses thereof
US6379930B1 (en) 1999-07-30 2002-04-30 Applied Gene Technologies, Inc. Stabilization of nucleic acid amplification cocktails
US6492103B1 (en) 2000-01-31 2002-12-10 Organ Recovery Systems, Inc. System for organ and tissue preservation and hypothermic blood substitution
DE10006662A1 (de) 2000-02-15 2001-08-23 Antigen Produktions Gmbh Gefäß zur Nukleinsäureanalytik
JP2003527210A (ja) 2000-03-23 2003-09-16 クリアラント・インコーポレイテッド 生体材料を滅菌するための方法
AU2001257117A1 (en) * 2000-04-18 2001-10-30 Tularik, Inc. Sitosterolemia susceptibility gene (ssg): compositions and methods of use
DE10031236A1 (de) 2000-06-27 2002-01-10 Qiagen Gmbh Verwendung von Carbonsäuren und anderen Additiven in Kombination mit kationischen Verbindungen zur Stabilisierung von Nukleinsäuren in biologischen Materialien
US6602718B1 (en) 2000-11-08 2003-08-05 Becton, Dickinson And Company Method and device for collecting and stabilizing a biological sample
ATE401574T1 (de) * 2001-08-23 2008-08-15 Immunivest Corp Analyse zirkulierender tumorzellen, fragmente und trümmer
US20030235588A1 (en) * 2002-02-15 2003-12-25 Richon Victoria M. Method of treating TRX mediated diseases
US6977162B2 (en) 2002-03-01 2005-12-20 Ravgen, Inc. Rapid analysis of variations in a genome
AU2002367863A1 (en) 2002-04-10 2003-10-27 Clearant, Inc. Methods for sterilizing biological materials
AU2003230270A1 (en) * 2002-05-07 2003-11-11 Becton, Dickinson And Company Collection assembly
US7442506B2 (en) 2002-05-08 2008-10-28 Ravgen, Inc. Methods for detection of genetic disorders
US20040054160A1 (en) 2002-09-16 2004-03-18 Santona Pal Nucleic-acid ink compositions for arraying onto a solid support
US20060212020A1 (en) 2002-10-10 2006-09-21 Lynne Rainen Sample collection system with caspase inhibitor
US20040167165A1 (en) * 2003-01-16 2004-08-26 Geetha Shankar Methods of treating conditions associated with an Edg-7 receptor
US7601491B2 (en) * 2003-02-06 2009-10-13 Becton, Dickinson And Company Pretreatment method for extraction of nucleic acid from biological samples and kits therefor
US7250270B2 (en) 2003-06-13 2007-07-31 Ambion, Inc. Methods and compositions for preparing tissue samples for RNA extraction
WO2005040355A2 (en) * 2003-10-24 2005-05-06 Exelixis, Inc. Tao kinase modulators and methods of use
US7270799B2 (en) 2004-01-15 2007-09-18 Nst Neurosurvival Technologies Ltd. Perturbed membrane-binding compounds and methods of using the same
WO2005081867A2 (en) 2004-02-20 2005-09-09 The Regents Of The University Of C Alifornia Salivary mrna profiling, biomarkers, and related methods and kits of parts
JP2007525224A (ja) * 2004-02-28 2007-09-06 チャン−ニン ジェイ. ワン 核酸複合体
US20080176209A1 (en) 2004-04-08 2008-07-24 Biomatrica, Inc. Integration of sample storage and sample management for life science
EP1758937B1 (en) 2004-05-24 2009-09-02 Genvault Corporation Stable protein storage and stable nucleic acid storage in recoverable form
US20100190688A1 (en) 2004-07-12 2010-07-29 Bin Chao Tetrapeptide analogs
US7086126B2 (en) * 2004-07-30 2006-08-08 Speaks, Inc. Elastic band with snap-buckles for holding garment waistband
FR2883003B1 (fr) 2005-03-14 2008-04-11 Aldivia Sa Nouveaux antioxydants a base d'especes d'anacardiacees, plus particulierement de sclerocarya birrea, leurs procedes d'obtention et leurs applications
DE102005015005A1 (de) 2005-04-01 2006-10-05 Qiagen Gmbh Verfahren zur Behandlung einer Biomoleküle enthaltenden Probe
AU2011253662A1 (en) 2005-11-30 2011-12-22 Massachusetts Institute Of Technology Pathogen-detecting cell preservation systems
EP1804045B1 (de) 2005-12-30 2014-03-12 QIAGEN GmbH Ein Verfahren und ein Kit zur Behandlung einer biologischen Probe
JP2009526754A (ja) 2006-01-04 2009-07-23 ドゥ−コープ テクノロジーズ リミテッド 医薬剤のインビボ取り込みを強化するための組成物および方法
US9745554B2 (en) 2006-01-20 2017-08-29 Reneuron, Inc. Method of improving cell proliferation of pancreatic progenitor cells in a pancreatic cell culture
US10925977B2 (en) * 2006-10-05 2021-02-23 Ceil>Point, LLC Efficient synthesis of chelators for nuclear imaging and radiotherapy: compositions and applications
GB0626021D0 (en) 2006-12-29 2007-02-07 Insense Ltd The stabilisation of proteins
US7828887B2 (en) 2007-04-23 2010-11-09 Hewlett-Packard Development Company, L.P. Dye-based ink formulations
JP5311760B2 (ja) 2007-05-25 2013-10-09 雪印メグミルク株式会社 美白剤
DE102007025277A1 (de) 2007-05-31 2008-12-04 Qiagen Gmbh Verfahren zur Stabilisierung einer biologischen Probe
EP2027922A1 (de) 2007-08-02 2009-02-25 Qiagen GmbH Verfahren und Vorrichtung für das Fixieren/Stabilisieren einer Probe
US20100285468A1 (en) 2007-09-24 2010-11-11 Allelogic Biosciences Corporation Detection and/or quantification of nucleic acids
EP2259798B1 (en) 2008-03-05 2013-12-11 Baxter International Inc. Surface-modified particles and methods for targeted drug delivery
WO2010065420A2 (en) 2008-12-03 2010-06-10 Integrated Nano-Technologies, Inc. Universal biological sample processing
US11634747B2 (en) * 2009-01-21 2023-04-25 Streck Llc Preservation of fetal nucleic acids in maternal plasma
US8304187B2 (en) 2009-02-18 2012-11-06 Streck, Inc. Preservation of cell-free RNA in blood samples
US20100280233A1 (en) 2009-05-04 2010-11-04 Connolly Dennis M Method for sample preparation
US20150056604A1 (en) 2009-06-15 2015-02-26 Biovec Transfusion, Llc Compositions and methods for preserving platelet function
US20110027771A1 (en) 2009-07-31 2011-02-03 Artemis Health, Inc. Methods and compositions for cell stabilization
US8039613B2 (en) 2009-08-28 2011-10-18 Promega Corporation Methods of purifying a nucleic acid and formulation and kit for use in performing such methods
US8222397B2 (en) * 2009-08-28 2012-07-17 Promega Corporation Methods of optimal purification of nucleic acids and kit for use in performing such methods
MX2012004499A (es) 2009-10-29 2012-05-29 Ascendis Pharma As Esterilizacion de hidrogeles biodegradables.
EP3719140A1 (en) 2009-11-06 2020-10-07 The Board Of Trustees Of The Leland Stanford Junior University Non-invasive diagnosis of graft rejection in organ transplant patients
CA2780536C (en) 2009-11-09 2018-01-02 Streck, Inc. Stabilization of rna in and extracting from intact cells within a blood sample
JP5773347B2 (ja) 2009-11-27 2015-09-02 国立大学法人 千葉大学 血液細胞の凍結保存剤
US8093427B2 (en) * 2010-06-09 2012-01-10 National Tsing Hua University Construction and screening of solution-phase derived library of fenbufen and ethacrynic acid
WO2011157678A1 (en) 2010-06-14 2011-12-22 Qiagen Gmbh Method for determination of target cells or tissue for extraction of biomolecules from fixed biological samples
FR2964567B1 (fr) 2010-09-14 2013-03-15 Lvmh Rech Extrait d'orchidee
US9403792B2 (en) 2011-05-03 2016-08-02 Agios Pharmaceuticals, Inc Methods of using pyruvate kinase activators
US20140243216A1 (en) 2011-09-26 2014-08-28 Qiagen Gmbh Methods for separating nucleic acids by size
CN113388605A (zh) 2011-09-26 2021-09-14 凯杰有限公司 用于分离细胞外核酸的快速方法
CN103827303B (zh) 2011-09-26 2021-10-22 普瑞阿那利提克斯有限公司 细胞外核酸的稳定化和分离
ES2971646T3 (es) 2011-09-26 2024-06-06 Preanalytix Gmbh Estabilización y aislamiento de ácidos nucleicos extracelulares
CN104066849B (zh) 2011-10-11 2017-04-19 凯杰有限公司 样品处理方法和样品处理盒
CN113786417A (zh) 2011-11-14 2021-12-14 安斯泰来再生医药协会 人rpe细胞的药物制剂及其用途
US9546358B2 (en) * 2012-06-04 2017-01-17 New England Biolabs, Inc. Compositions and methods for reducing background DNA amplification
CN104755628B (zh) 2012-09-25 2019-03-01 凯杰有限公司 生物样品的稳定化
WO2014055936A1 (en) 2012-10-04 2014-04-10 Integenx Inc. Preservation of biological materials in non-aqueous fluid media
GB201303666D0 (en) 2013-03-01 2013-04-17 Goldsborough Andrew S Sample fixation and stabilisation
US11525155B2 (en) 2013-03-18 2022-12-13 Qiagen Gmbh Stabilisation of biological samples
US20160281133A1 (en) 2013-03-18 2016-09-29 Qiagen Gmbh Stabilization and isolation of extracellular nucleic acids
JP6664332B2 (ja) 2014-03-18 2020-03-13 キアゲン ゲーエムベーハー 細胞外核酸の安定化および単離
EP3698628A1 (en) 2014-08-07 2020-08-26 The General Hospital Corporation Stabilization of whole blood samples

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Publication number Publication date
WO2014146782A1 (en) 2014-09-25
EP2976425B1 (en) 2019-11-06
WO2014146780A1 (en) 2014-09-25
CN105164258A (zh) 2015-12-16
CN105164258B (zh) 2021-05-18
US20160281132A1 (en) 2016-09-29
US20160281133A1 (en) 2016-09-29
JP2016520293A (ja) 2016-07-14
JP2016518827A (ja) 2016-06-30
EP2976425A1 (en) 2016-01-27
EP2976426A1 (en) 2016-01-27
CN105121643A (zh) 2015-12-02
US10144952B2 (en) 2018-12-04

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