JP6349462B2 - 可溶性インテイン融合タンパク質、及び生体分子を精製する方法 - Google Patents

可溶性インテイン融合タンパク質、及び生体分子を精製する方法 Download PDF

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JP6349462B2
JP6349462B2 JP2017521137A JP2017521137A JP6349462B2 JP 6349462 B2 JP6349462 B2 JP 6349462B2 JP 2017521137 A JP2017521137 A JP 2017521137A JP 2017521137 A JP2017521137 A JP 2017521137A JP 6349462 B2 JP6349462 B2 JP 6349462B2
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intein
fusion protein
polypeptide
solid support
seq
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JP2017533701A (ja
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ジルマン，マーティン
オーランド，ジョー
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メルク パテント ゲーエムベーハー
メルク パテント ゲーエムベーハー
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/38—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving specific interaction not covered by one or more of groups B01D15/265 and B01D15/30 - B01D15/36, e.g. affinity, ligand exchange or chiral chromatography
    • B01D15/3804—Affinity chromatography
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/22—Affinity chromatography or related techniques based upon selective absorption processes
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
    • C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
    • C12N11/08—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a synthetic polymer
    • C12N11/082—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a synthetic polymer obtained by reactions only involving carbon-to-carbon unsaturated bonds
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
    • C12N11/14—Enzymes or microbial cells immobilised on or in an inorganic carrier
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/93—Ligases (6)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/90—Fusion polypeptide containing a motif for post-translational modification
    • C07K2319/92—Fusion polypeptide containing a motif for post-translational modification containing an intein ("protein splicing")domain

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Immunology (AREA)
  • Inorganic Chemistry (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
JP2017521137A 2014-11-03 2015-10-23 可溶性インテイン融合タンパク質、及び生体分子を精製する方法 Active JP6349462B2 (ja)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US201462074494P 2014-11-03 2014-11-03
US62/074,494 2014-11-03
US201562209010P 2015-08-24 2015-08-24
US62/209,010 2015-08-24
PCT/US2015/057125 WO2016073228A1 (en) 2014-11-03 2015-10-23 Soluble intein fusion proteins and methods for purifying biomolecules

Related Child Applications (1)

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JP2018107188A Division JP7058560B2 (ja) 2014-11-03 2018-06-04 可溶性インテイン融合タンパク質、及び生体分子を精製する方法

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JP2017533701A JP2017533701A (ja) 2017-11-16
JP6349462B2 true JP6349462B2 (ja) 2018-06-27

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JP2017521137A Active JP6349462B2 (ja) 2014-11-03 2015-10-23 可溶性インテイン融合タンパク質、及び生体分子を精製する方法
JP2018107188A Active JP7058560B2 (ja) 2014-11-03 2018-06-04 可溶性インテイン融合タンパク質、及び生体分子を精製する方法
JP2020136598A Active JP7054722B2 (ja) 2014-11-03 2020-08-13 可溶性インテイン融合タンパク質、及び生体分子を精製する方法

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JP2020136598A Active JP7054722B2 (ja) 2014-11-03 2020-08-13 可溶性インテイン融合タンパク質、及び生体分子を精製する方法

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US (3) US10308679B2 (enExample)
EP (2) EP3215614B1 (enExample)
JP (3) JP6349462B2 (enExample)
KR (3) KR101996774B1 (enExample)
CN (1) CN107406514B (enExample)
CA (1) CA2964119C (enExample)
ES (1) ES2742199T3 (enExample)
PL (1) PL3215614T3 (enExample)
SG (2) SG11201701965VA (enExample)
SI (1) SI3215614T1 (enExample)
WO (1) WO2016073228A1 (enExample)

Families Citing this family (12)

* Cited by examiner, † Cited by third party
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SI3215614T1 (sl) 2014-11-03 2019-11-29 Merck Patent Gmbh Topni fuzijski proteini inteina in postopki za čiščenje biomolekul
KR102000490B1 (ko) * 2018-01-12 2019-10-01 전남대학교산학협력단 가용성이 개선된 살모넬라균 편모 유래 플라젤린 단백질 발현 형질전환체, 그 제조방법 및 용도
WO2020053239A1 (en) * 2018-09-11 2020-03-19 Nanotag Biotechnologies Gmbh Epitope tags recognized by specific binders
CN113195521B (zh) 2018-12-19 2023-05-12 清华大学 Mtu ΔI-CM内含肽变体和其应用
KR102129377B1 (ko) * 2019-02-21 2020-07-02 성균관대학교산학협력단 특정 특성이 발현된 단백질을 암호화하는 플라스미드를 선별하는 유도 플라스미드 디스플레이 시스템
CN110201419B (zh) * 2019-05-09 2021-01-19 西安交通大学 一种以聚乙烯醇微球为载体的细胞膜色谱柱及其制备方法
GB201917046D0 (en) 2019-11-22 2020-01-08 Ge Healthcare Bioprocess R&D Ab Improved protein production
US12503488B2 (en) 2020-03-25 2025-12-23 Merck Patent Gmbh Reuse of intein-bound resins for protein purification
US20230391821A1 (en) * 2020-09-28 2023-12-07 Merck Patent Gmbh Methods for target release from intein complexes
WO2022238319A1 (en) 2021-05-12 2022-11-17 Cytiva Bioprocess R&D Ab Improved protein purification
CN116041451B (zh) * 2022-08-15 2023-08-15 广州市乾相生物科技有限公司 一种内含肽变体及其在生物法制备蓝铜胜肽中的应用
CN116731126B (zh) * 2023-01-30 2024-02-23 态创生物科技(广州)有限公司 内含肽ChiATP、内含肽ChiATP-二肽-2融合蛋白及二肽-2的表达方法

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JP3952347B2 (ja) * 1999-04-16 2007-08-01 株式会社リコー 搬送装置
FR2803913B1 (fr) 2000-01-13 2002-08-16 Pasteur Sanofi Diagnostics Procede d'immobilisation de reactif(s) affin(s) sur phase solide hydrophobe
US6562576B2 (en) 2001-01-04 2003-05-13 Myriad Genetics, Inc. Yeast two-hybrid system and use thereof
US8362217B2 (en) 2006-12-21 2013-01-29 Emd Millipore Corporation Purification of proteins
AU2008209404B2 (en) 2007-01-22 2012-08-16 Genentech, Inc. Polyelectrolyte precipitation and purification of antibodies
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EA021205B1 (ru) 2009-03-10 2015-04-30 ДСМ АйПи АССЕТС Б.В. Способ повышения выхода полипептидов
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Publication number Publication date
US20220267750A1 (en) 2022-08-25
US20190241609A1 (en) 2019-08-08
SG11201701965VA (en) 2017-05-30
EP3543335A3 (en) 2019-11-27
PL3215614T3 (pl) 2019-12-31
KR20180095125A (ko) 2018-08-24
KR102105352B1 (ko) 2020-04-29
JP2020180162A (ja) 2020-11-05
KR20170067901A (ko) 2017-06-16
JP2017533701A (ja) 2017-11-16
JP7058560B2 (ja) 2022-04-22
EP3215614B1 (en) 2019-05-29
KR101996774B1 (ko) 2019-07-04
SI3215614T1 (sl) 2019-11-29
CA2964119A1 (en) 2016-05-12
US11326154B2 (en) 2022-05-10
CN107406514A (zh) 2017-11-28
KR20190077620A (ko) 2019-07-03
KR101891455B1 (ko) 2018-08-27
JP7054722B2 (ja) 2022-04-14
EP3543335A2 (en) 2019-09-25
EP3215614A1 (en) 2017-09-13
US20170291919A1 (en) 2017-10-12
US11926854B2 (en) 2024-03-12
WO2016073228A1 (en) 2016-05-12
CN107406514B (zh) 2021-11-30
CA2964119C (en) 2022-10-18
ES2742199T3 (es) 2020-02-13
US10308679B2 (en) 2019-06-04
JP2018141017A (ja) 2018-09-13
SG10201801320UA (en) 2018-04-27

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