JP6347949B2 - 改良型細胞培養培地 - Google Patents

改良型細胞培養培地 Download PDF

Info

Publication number
JP6347949B2
JP6347949B2 JP2013506612A JP2013506612A JP6347949B2 JP 6347949 B2 JP6347949 B2 JP 6347949B2 JP 2013506612 A JP2013506612 A JP 2013506612A JP 2013506612 A JP2013506612 A JP 2013506612A JP 6347949 B2 JP6347949 B2 JP 6347949B2
Authority
JP
Japan
Prior art keywords
cells
medium
cell culture
culture
cell
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
JP2013506612A
Other languages
English (en)
Japanese (ja)
Other versions
JP2013524825A5 (enExample
JP2013524825A (ja
Inventor
ライスト,クリスチャン
マイズナー,ペトラ
シュミト,ヨルグ
Original Assignee
ノバルティス アーゲー
ノバルティス アーゲー
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=44074260&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=JP6347949(B2) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by ノバルティス アーゲー, ノバルティス アーゲー filed Critical ノバルティス アーゲー
Publication of JP2013524825A publication Critical patent/JP2013524825A/ja
Publication of JP2013524825A5 publication Critical patent/JP2013524825A5/ja
Application granted granted Critical
Publication of JP6347949B2 publication Critical patent/JP6347949B2/ja
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Images

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0018Culture media for cell or tissue culture
    • C12N5/0031Serum-free culture media
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0018Culture media for cell or tissue culture
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0018Culture media for cell or tissue culture
    • C12N5/005Protein-free medium
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K1/00General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K16/00Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00Specific components of cell culture medium
    • C12N2500/05Inorganic components
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00Specific components of cell culture medium
    • C12N2500/05Inorganic components
    • C12N2500/10Metals; Metal chelators
    • C12N2500/12Light metals, i.e. alkali, alkaline earth, Be, Al, Mg

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • Cell Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
JP2013506612A 2010-04-26 2011-04-25 改良型細胞培養培地 Active JP6347949B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US32783610P 2010-04-26 2010-04-26
US61/327,836 2010-04-26
PCT/EP2011/056508 WO2011134920A1 (en) 2010-04-26 2011-04-25 Improved cell culture medium

Related Child Applications (1)

Application Number Title Priority Date Filing Date
JP2016152747A Division JP6603628B2 (ja) 2010-04-26 2016-08-03 改良型細胞培養培地

Publications (3)

Publication Number Publication Date
JP2013524825A JP2013524825A (ja) 2013-06-20
JP2013524825A5 JP2013524825A5 (enExample) 2014-06-05
JP6347949B2 true JP6347949B2 (ja) 2018-06-27

Family

ID=44074260

Family Applications (3)

Application Number Title Priority Date Filing Date
JP2013506612A Active JP6347949B2 (ja) 2010-04-26 2011-04-25 改良型細胞培養培地
JP2016152747A Active JP6603628B2 (ja) 2010-04-26 2016-08-03 改良型細胞培養培地
JP2019142124A Active JP7034991B2 (ja) 2010-04-26 2019-08-01 改良型細胞培養培地

Family Applications After (2)

Application Number Title Priority Date Filing Date
JP2016152747A Active JP6603628B2 (ja) 2010-04-26 2016-08-03 改良型細胞培養培地
JP2019142124A Active JP7034991B2 (ja) 2010-04-26 2019-08-01 改良型細胞培養培地

Country Status (17)

Country Link
US (2) USRE48864E1 (enExample)
EP (1) EP2563904B1 (enExample)
JP (3) JP6347949B2 (enExample)
KR (1) KR101828623B1 (enExample)
CN (1) CN102858952B (enExample)
AU (1) AU2011246503B2 (enExample)
BR (1) BR112012027282B1 (enExample)
CA (1) CA2797356C (enExample)
DK (1) DK2563904T3 (enExample)
ES (1) ES2533074T3 (enExample)
IL (1) IL222452B (enExample)
MX (1) MX2012012526A (enExample)
PL (1) PL2563904T3 (enExample)
PT (1) PT2563904E (enExample)
RU (1) RU2644651C2 (enExample)
SG (1) SG185038A1 (enExample)
WO (1) WO2011134920A1 (enExample)

Families Citing this family (45)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2500413A1 (en) 2006-09-13 2012-09-19 Abbott Laboratories Cell culture improvements
US8911964B2 (en) 2006-09-13 2014-12-16 Abbvie Inc. Fed-batch method of making human anti-TNF-alpha antibody
US8895709B2 (en) 2008-10-20 2014-11-25 Abbvie Inc. Isolation and purification of antibodies using protein A affinity chromatography
NZ592097A (en) 2008-10-20 2013-01-25 Abbott Lab Viral inactivation during purification of il-12 and il-18 antibodies
ES2755907T3 (es) 2010-04-26 2020-04-24 Novartis Ag Medio de cultivo celular mejorado
BR112012027282B1 (pt) 2010-04-26 2020-09-29 Novartis Ag Meio de cultura de células aperfeiçoado e processo para a produção de um polipeptídeo recombinante
JP5824527B2 (ja) * 2010-12-07 2015-11-25 エフ.ホフマン−ラ ロシュ アーゲーF. Hoffmann−La Roche Aktiengesellschaft 供給物混合装置およびその使用
EP2702077A2 (en) 2011-04-27 2014-03-05 AbbVie Inc. Methods for controlling the galactosylation profile of recombinantly-expressed proteins
US10493151B2 (en) 2011-10-18 2019-12-03 Coherus Biosciences, Inc. Etanercept formulations stabilized with sodium chloride
KR20140091706A (ko) 2011-10-18 2014-07-22 코히러스 바이오사이언시즈, 인코포레이티드 염화나트륨으로 안정화된 에타너셉트 제형
US9505833B2 (en) 2012-04-20 2016-11-29 Abbvie Inc. Human antibodies that bind human TNF-alpha and methods of preparing the same
US9067990B2 (en) 2013-03-14 2015-06-30 Abbvie, Inc. Protein purification using displacement chromatography
WO2013158273A1 (en) 2012-04-20 2013-10-24 Abbvie Inc. Methods to modulate c-terminal lysine variant distribution
WO2013158279A1 (en) 2012-04-20 2013-10-24 Abbvie Inc. Protein purification methods to reduce acidic species
US20130281355A1 (en) 2012-04-24 2013-10-24 Genentech, Inc. Cell culture compositions and methods for polypeptide production
WO2013176754A1 (en) 2012-05-24 2013-11-28 Abbvie Inc. Novel purification of antibodies using hydrophobic interaction chromatography
EP2869817A4 (en) 2012-07-09 2016-04-06 Coherus Biosciences Inc STABLE AQUEOUS FORMULATIONS OF ETANERCEPT
US9512214B2 (en) 2012-09-02 2016-12-06 Abbvie, Inc. Methods to control protein heterogeneity
SG11201504249XA (en) 2012-09-02 2015-07-30 Abbvie Inc Methods to control protein heterogeneity
HUE036524T2 (hu) 2012-09-11 2018-07-30 Coherus Biosciences Inc Nagy tisztaságú és kiváló kitermelésû helyesen hajtogatott etanercept
US9017687B1 (en) 2013-10-18 2015-04-28 Abbvie, Inc. Low acidic species compositions and methods for producing and using the same using displacement chromatography
US8921526B2 (en) 2013-03-14 2014-12-30 Abbvie, Inc. Mutated anti-TNFα antibodies and methods of their use
CA2899308C (en) 2013-03-14 2017-04-18 Abbvie Inc. Low acidic species adalimumab compositions and uses thereof
AU2014310555B2 (en) 2013-08-20 2018-01-18 Lek Pharmaceuticals D.D. Cell culture medium and process for controlling alpha-amidation and/or C-terminal amino acid cleavage of polypeptides
US9598667B2 (en) 2013-10-04 2017-03-21 Abbvie Inc. Use of metal ions for modulation of protein glycosylation profiles of recombinant proteins
SG10201800772TA (en) * 2013-10-11 2018-03-28 Regeneron Pharma Metabolically optimized cell culture
US11390663B2 (en) 2013-10-11 2022-07-19 Regeneron Pharmaceuticals, Inc. Metabolically optimized cell culture
US8946395B1 (en) 2013-10-18 2015-02-03 Abbvie Inc. Purification of proteins using hydrophobic interaction chromatography
US9085618B2 (en) 2013-10-18 2015-07-21 Abbvie, Inc. Low acidic species compositions and methods for producing and using the same
US9181337B2 (en) 2013-10-18 2015-11-10 Abbvie, Inc. Modulated lysine variant species compositions and methods for producing and using the same
WO2015073884A2 (en) 2013-11-15 2015-05-21 Abbvie, Inc. Glycoengineered binding protein compositions
EP3683316A1 (en) 2014-01-30 2020-07-22 Coherus Biosciences, Inc. Perfusion media
MX368664B (es) 2014-04-29 2019-10-10 Novartis Ag Células novedosas de vertebrados y métodos para la expresión recombinante de un polipéptido de interés.
EP2975050B1 (en) 2014-07-18 2019-05-29 Sandoz Ag Quantification of misfolded TNFR2:Fc
RU2759945C2 (ru) * 2015-12-17 2021-11-19 Лайф Текнолоджиз Корпорейшн Гранулы, используемые в клеточной культуре, и способы их получения
CA3019753C (en) * 2016-04-05 2021-10-26 Pfizer Inc. Cell culture process
JP6943972B2 (ja) * 2017-03-31 2021-10-06 ベーリンガー インゲルハイム インターナショナル ゲゼルシャフト ミット ベシュレンクテル ハフツング 灌流培地
SG11201908891YA (en) * 2017-03-31 2019-10-30 Boehringer Ingelheim Int Perfusion medium
AU2020261411B2 (en) 2019-04-26 2025-10-02 Allogene Therapeutics, Inc. Methods of manufacturing allogeneic car T cells
US12297451B1 (en) 2019-10-25 2025-05-13 Regeneron Pharmaceuticals, Inc. Cell culture medium
EP4056678A4 (en) * 2019-11-05 2023-12-06 Ajinomoto Co., Inc. Method for producing protein
IL297191A (en) * 2020-04-15 2022-12-01 Genentech Inc A method for treating the medium before inoculation
JP2023537760A (ja) * 2020-08-14 2023-09-05 ブリストル-マイヤーズ スクイブ カンパニー タンパク質の製造工程
UY39484A (es) 2020-11-02 2022-06-30 Novartis Ag Inhibidores de interleucina-17
CN114606200B (zh) * 2022-02-10 2025-07-18 上海迈邦生物科技有限公司 Sp2/0细胞培养基及生产重组犬细小病毒单克隆抗体的方法

Family Cites Families (33)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5672502A (en) 1985-06-28 1997-09-30 Celltech Therapeutics Limited Animal cell culture
EP0283942B1 (en) 1987-03-24 1992-05-20 W.R. Grace & Co.-Conn. Basal nutrient medium for cell culture
US6048728A (en) * 1988-09-23 2000-04-11 Chiron Corporation Cell culture medium for enhanced cell growth, culture longevity, and product expression
US5135866A (en) 1989-03-03 1992-08-04 W. R. Grace & Co.-Conn. Very low protein nutrient medium for cell culture
CA2001550A1 (en) 1989-03-03 1990-09-03 Aaron H. Heifetz Very low protein nutrient medium for cell culture
US5122469A (en) 1990-10-03 1992-06-16 Genentech, Inc. Method for culturing Chinese hamster ovary cells to improve production of recombinant proteins
GB9022545D0 (en) 1990-10-17 1990-11-28 Wellcome Found Culture medium
JPH0789908B2 (ja) 1991-02-28 1995-10-04 倉敷紡績株式会社 動物細胞培養用無血清培地
GB9118664D0 (en) * 1991-08-30 1991-10-16 Celltech Ltd Cell culture
US5856179A (en) 1994-03-10 1999-01-05 Genentech, Inc. Polypeptide production in animal cell culture
US5830761A (en) 1995-06-07 1998-11-03 Genetics Institute, Inc. Medium and methods for culturing mammalian cho cells
AU4330597A (en) 1996-08-30 1998-03-19 Life Technologies, Inc. Serum-free mammalian cell culture medium, and uses thereof
US5804420A (en) 1997-04-18 1998-09-08 Bayer Corporation Preparation of recombinant Factor VIII in a protein free medium
US6406909B1 (en) 1998-07-10 2002-06-18 Chugai Seiyaku Kabushiki Kaisha Serum-free medium for culturing animal cells
US7294481B1 (en) 1999-01-05 2007-11-13 Immunex Corporation Method for producing recombinant proteins
EP1757700A3 (en) 1999-04-26 2009-03-11 Genentech, Inc. Cell culture process for glycoproteins
WO2000073431A2 (en) 1999-05-27 2000-12-07 Pharmacia & Upjohn Company Methods and compositions for measuring ion channel conductance
AT409379B (de) * 1999-06-02 2002-07-25 Baxter Ag Medium zur protein- und serumfreien kultivierung von zellen
DK1242578T3 (da) 1999-12-28 2004-07-26 Iso Tis N V Celledyrkningsmedium indeholdende vækstfaktorer og L-glutamin
ATE420945T1 (de) 2001-02-15 2009-01-15 Centocor Inc Chemisch definiertes medium für kultivierte säugerzellen
WO2002101019A2 (en) 2001-06-13 2002-12-19 Genentech, Inc. Methods of culturing animal cells and polypeptide production in animal cells
JP4510938B2 (ja) 2002-07-15 2010-07-28 イミュネックス・コーポレーション 哺乳動物細胞により産生されるタンパク質のシアル酸付加を調節する方法および培地
KR20050088136A (ko) 2002-12-23 2005-09-01 브리스톨-마이어스 스큅 컴퍼니 단백질 생산을 위한 포유류 세포 배양 방법
GB0304799D0 (en) * 2003-03-03 2003-04-09 Glaxosmithkline Biolog Sa Novel method
DE10338536A1 (de) 2003-08-19 2005-04-07 Bircher Reglomat Ag Verfahren zum Betreiben eines Radarsensors
US7335491B2 (en) 2004-08-27 2008-02-26 Wyeth Research Ireland Limited Production of anti-abeta
US7294484B2 (en) * 2004-08-27 2007-11-13 Wyeth Research Ireland Limited Production of polypeptides
EP1707634A1 (en) 2005-03-29 2006-10-04 Octapharma AG Method for isolation of recombinantly produced proteins
WO2006113629A1 (en) 2005-04-15 2006-10-26 The Trustees Of Columbia University In The City Of New York Chemically defined culture media for expansion and differentiation of epidermal cells and uses thereof for in vitro growth of hair follicles
US20070231895A1 (en) 2005-11-02 2007-10-04 Lee Gene W Methods for adapting mammalian cells
US20070190057A1 (en) 2006-01-23 2007-08-16 Jian Wu Methods for modulating mannose content of recombinant proteins
PL2078071T3 (pl) * 2006-11-08 2015-07-31 Wyeth Llc Racjonalnie opracowane pożywki do hodowli komórek
BR112012027282B1 (pt) 2010-04-26 2020-09-29 Novartis Ag Meio de cultura de células aperfeiçoado e processo para a produção de um polipeptídeo recombinante

Also Published As

Publication number Publication date
KR20130058699A (ko) 2013-06-04
JP6603628B2 (ja) 2019-11-06
USRE48864E1 (en) 2021-12-28
BR112012027282B1 (pt) 2020-09-29
EP2563904B1 (en) 2015-01-21
JP7034991B2 (ja) 2022-03-14
US20130122543A1 (en) 2013-05-16
BR112012027282A2 (pt) 2015-09-22
ES2533074T3 (es) 2015-04-07
JP2019216728A (ja) 2019-12-26
RU2644651C2 (ru) 2018-02-13
PL2563904T3 (pl) 2015-06-30
EP2563904A1 (en) 2013-03-06
IL222452B (en) 2019-03-31
AU2011246503B2 (en) 2013-09-26
RU2018101201A3 (enExample) 2021-05-27
MX2012012526A (es) 2012-11-23
KR101828623B1 (ko) 2018-02-12
CA2797356A1 (en) 2011-11-03
PT2563904E (pt) 2015-05-12
CA2797356C (en) 2020-12-29
US9428727B2 (en) 2016-08-30
CN102858952A (zh) 2013-01-02
JP2013524825A (ja) 2013-06-20
AU2011246503A1 (en) 2012-11-22
WO2011134920A1 (en) 2011-11-03
RU2012150346A (ru) 2014-06-10
JP2017012180A (ja) 2017-01-19
CN102858952B (zh) 2015-10-14
SG185038A1 (en) 2012-11-29
RU2018101201A (ru) 2019-02-21
DK2563904T3 (en) 2015-04-07
IL222452A0 (en) 2012-12-31

Similar Documents

Publication Publication Date Title
JP6603628B2 (ja) 改良型細胞培養培地
KR101828624B1 (ko) 개선된 세포 배양 방법
KR101362805B1 (ko) 개선된 세포 배양 배지
KR101848945B1 (ko) 개선된 세포 배양 배지
RU2798069C2 (ru) Улучшенная среда для культивирования клеток

Legal Events

Date Code Title Description
A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20140415

A621 Written request for application examination

Free format text: JAPANESE INTERMEDIATE CODE: A621

Effective date: 20140415

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20150630

A601 Written request for extension of time

Free format text: JAPANESE INTERMEDIATE CODE: A601

Effective date: 20150915

A601 Written request for extension of time

Free format text: JAPANESE INTERMEDIATE CODE: A601

Effective date: 20151102

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20151125

A02 Decision of refusal

Free format text: JAPANESE INTERMEDIATE CODE: A02

Effective date: 20160405

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20160803

A911 Transfer to examiner for re-examination before appeal (zenchi)

Free format text: JAPANESE INTERMEDIATE CODE: A911

Effective date: 20160921

A912 Re-examination (zenchi) completed and case transferred to appeal board

Free format text: JAPANESE INTERMEDIATE CODE: A912

Effective date: 20161118

A601 Written request for extension of time

Free format text: JAPANESE INTERMEDIATE CODE: A601

Effective date: 20171128

A601 Written request for extension of time

Free format text: JAPANESE INTERMEDIATE CODE: A601

Effective date: 20180125

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20180228

A61 First payment of annual fees (during grant procedure)

Free format text: JAPANESE INTERMEDIATE CODE: A61

Effective date: 20180530

R150 Certificate of patent or registration of utility model

Ref document number: 6347949

Country of ref document: JP

Free format text: JAPANESE INTERMEDIATE CODE: R150

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250