JP2013524825A5 - - Google Patents
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- Publication number
- JP2013524825A5 JP2013524825A5 JP2013506612A JP2013506612A JP2013524825A5 JP 2013524825 A5 JP2013524825 A5 JP 2013524825A5 JP 2013506612 A JP2013506612 A JP 2013506612A JP 2013506612 A JP2013506612 A JP 2013506612A JP 2013524825 A5 JP2013524825 A5 JP 2013524825A5
- Authority
- JP
- Japan
- Prior art keywords
- cell culture
- culture medium
- concentration
- cells
- molar ratio
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 239000006143 cell culture medium Substances 0.000 claims 8
- 238000000034 method Methods 0.000 claims 8
- 229920001184 polypeptide Polymers 0.000 claims 4
- 102000004196 processed proteins & peptides Human genes 0.000 claims 4
- 108090000765 processed proteins & peptides Proteins 0.000 claims 4
- 210000004962 mammalian cell Anatomy 0.000 claims 3
- 229910001414 potassium ion Inorganic materials 0.000 claims 3
- 229910001415 sodium ion Inorganic materials 0.000 claims 3
- WCUXLLCKKVVCTQ-UHFFFAOYSA-M Potassium chloride Chemical compound [Cl-].[K+] WCUXLLCKKVVCTQ-UHFFFAOYSA-M 0.000 claims 2
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims 2
- 150000001413 amino acids Chemical class 0.000 claims 2
- 210000004027 cell Anatomy 0.000 claims 2
- 239000002609 medium Substances 0.000 claims 2
- 102000008394 Immunoglobulin Fragments Human genes 0.000 claims 1
- 108010021625 Immunoglobulin Fragments Proteins 0.000 claims 1
- NPYPAHLBTDXSSS-UHFFFAOYSA-N Potassium ion Chemical compound [K+] NPYPAHLBTDXSSS-UHFFFAOYSA-N 0.000 claims 1
- FKNQFGJONOIPTF-UHFFFAOYSA-N Sodium cation Chemical compound [Na+] FKNQFGJONOIPTF-UHFFFAOYSA-N 0.000 claims 1
- 238000010923 batch production Methods 0.000 claims 1
- 210000004978 chinese hamster ovary cell Anatomy 0.000 claims 1
- 238000012258 culturing Methods 0.000 claims 1
- 150000002500 ions Chemical class 0.000 claims 1
- 238000004519 manufacturing process Methods 0.000 claims 1
- 239000001103 potassium chloride Substances 0.000 claims 1
- 235000011164 potassium chloride Nutrition 0.000 claims 1
- 239000011780 sodium chloride Substances 0.000 claims 1
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US32783610P | 2010-04-26 | 2010-04-26 | |
| US61/327,836 | 2010-04-26 | ||
| PCT/EP2011/056508 WO2011134920A1 (en) | 2010-04-26 | 2011-04-25 | Improved cell culture medium |
Related Child Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2016152747A Division JP6603628B2 (ja) | 2010-04-26 | 2016-08-03 | 改良型細胞培養培地 |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2013524825A JP2013524825A (ja) | 2013-06-20 |
| JP2013524825A5 true JP2013524825A5 (enExample) | 2014-06-05 |
| JP6347949B2 JP6347949B2 (ja) | 2018-06-27 |
Family
ID=44074260
Family Applications (3)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2013506612A Active JP6347949B2 (ja) | 2010-04-26 | 2011-04-25 | 改良型細胞培養培地 |
| JP2016152747A Active JP6603628B2 (ja) | 2010-04-26 | 2016-08-03 | 改良型細胞培養培地 |
| JP2019142124A Active JP7034991B2 (ja) | 2010-04-26 | 2019-08-01 | 改良型細胞培養培地 |
Family Applications After (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2016152747A Active JP6603628B2 (ja) | 2010-04-26 | 2016-08-03 | 改良型細胞培養培地 |
| JP2019142124A Active JP7034991B2 (ja) | 2010-04-26 | 2019-08-01 | 改良型細胞培養培地 |
Country Status (17)
| Country | Link |
|---|---|
| US (2) | USRE48864E1 (enExample) |
| EP (1) | EP2563904B1 (enExample) |
| JP (3) | JP6347949B2 (enExample) |
| KR (1) | KR101828623B1 (enExample) |
| CN (1) | CN102858952B (enExample) |
| AU (1) | AU2011246503B2 (enExample) |
| BR (1) | BR112012027282B1 (enExample) |
| CA (1) | CA2797356C (enExample) |
| DK (1) | DK2563904T3 (enExample) |
| ES (1) | ES2533074T3 (enExample) |
| IL (1) | IL222452B (enExample) |
| MX (1) | MX2012012526A (enExample) |
| PL (1) | PL2563904T3 (enExample) |
| PT (1) | PT2563904E (enExample) |
| RU (1) | RU2644651C2 (enExample) |
| SG (1) | SG185038A1 (enExample) |
| WO (1) | WO2011134920A1 (enExample) |
Families Citing this family (45)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP2500413A1 (en) | 2006-09-13 | 2012-09-19 | Abbott Laboratories | Cell culture improvements |
| US8911964B2 (en) | 2006-09-13 | 2014-12-16 | Abbvie Inc. | Fed-batch method of making human anti-TNF-alpha antibody |
| US8895709B2 (en) | 2008-10-20 | 2014-11-25 | Abbvie Inc. | Isolation and purification of antibodies using protein A affinity chromatography |
| NZ592097A (en) | 2008-10-20 | 2013-01-25 | Abbott Lab | Viral inactivation during purification of il-12 and il-18 antibodies |
| ES2755907T3 (es) | 2010-04-26 | 2020-04-24 | Novartis Ag | Medio de cultivo celular mejorado |
| BR112012027282B1 (pt) | 2010-04-26 | 2020-09-29 | Novartis Ag | Meio de cultura de células aperfeiçoado e processo para a produção de um polipeptídeo recombinante |
| JP5824527B2 (ja) * | 2010-12-07 | 2015-11-25 | エフ．ホフマン−ラ ロシュ アーゲーＦ． Ｈｏｆｆｍａｎｎ−Ｌａ Ｒｏｃｈｅ Ａｋｔｉｅｎｇｅｓｅｌｌｓｃｈａｆｔ | 供給物混合装置およびその使用 |
| EP2702077A2 (en) | 2011-04-27 | 2014-03-05 | AbbVie Inc. | Methods for controlling the galactosylation profile of recombinantly-expressed proteins |
| US10493151B2 (en) | 2011-10-18 | 2019-12-03 | Coherus Biosciences, Inc. | Etanercept formulations stabilized with sodium chloride |
| KR20140091706A (ko) | 2011-10-18 | 2014-07-22 | 코히러스 바이오사이언시즈, 인코포레이티드 | 염화나트륨으로 안정화된 에타너셉트 제형 |
| US9505833B2 (en) | 2012-04-20 | 2016-11-29 | Abbvie Inc. | Human antibodies that bind human TNF-alpha and methods of preparing the same |
| US9067990B2 (en) | 2013-03-14 | 2015-06-30 | Abbvie, Inc. | Protein purification using displacement chromatography |
| WO2013158273A1 (en) | 2012-04-20 | 2013-10-24 | Abbvie Inc. | Methods to modulate c-terminal lysine variant distribution |
| WO2013158279A1 (en) | 2012-04-20 | 2013-10-24 | Abbvie Inc. | Protein purification methods to reduce acidic species |
| US20130281355A1 (en) | 2012-04-24 | 2013-10-24 | Genentech, Inc. | Cell culture compositions and methods for polypeptide production |
| WO2013176754A1 (en) | 2012-05-24 | 2013-11-28 | Abbvie Inc. | Novel purification of antibodies using hydrophobic interaction chromatography |
| EP2869817A4 (en) | 2012-07-09 | 2016-04-06 | Coherus Biosciences Inc | STABLE AQUEOUS FORMULATIONS OF ETANERCEPT |
| US9512214B2 (en) | 2012-09-02 | 2016-12-06 | Abbvie, Inc. | Methods to control protein heterogeneity |
| SG11201504249XA (en) | 2012-09-02 | 2015-07-30 | Abbvie Inc | Methods to control protein heterogeneity |
| HUE036524T2 (hu) | 2012-09-11 | 2018-07-30 | Coherus Biosciences Inc | Nagy tisztaságú és kiváló kitermelésû helyesen hajtogatott etanercept |
| US9017687B1 (en) | 2013-10-18 | 2015-04-28 | Abbvie, Inc. | Low acidic species compositions and methods for producing and using the same using displacement chromatography |
| US8921526B2 (en) | 2013-03-14 | 2014-12-30 | Abbvie, Inc. | Mutated anti-TNFα antibodies and methods of their use |
| CA2899308C (en) | 2013-03-14 | 2017-04-18 | Abbvie Inc. | Low acidic species adalimumab compositions and uses thereof |
| AU2014310555B2 (en) | 2013-08-20 | 2018-01-18 | Lek Pharmaceuticals D.D. | Cell culture medium and process for controlling alpha-amidation and/or C-terminal amino acid cleavage of polypeptides |
| US9598667B2 (en) | 2013-10-04 | 2017-03-21 | Abbvie Inc. | Use of metal ions for modulation of protein glycosylation profiles of recombinant proteins |
| SG10201800772TA (en) * | 2013-10-11 | 2018-03-28 | Regeneron Pharma | Metabolically optimized cell culture |
| US11390663B2 (en) | 2013-10-11 | 2022-07-19 | Regeneron Pharmaceuticals, Inc. | Metabolically optimized cell culture |
| US8946395B1 (en) | 2013-10-18 | 2015-02-03 | Abbvie Inc. | Purification of proteins using hydrophobic interaction chromatography |
| US9085618B2 (en) | 2013-10-18 | 2015-07-21 | Abbvie, Inc. | Low acidic species compositions and methods for producing and using the same |
| US9181337B2 (en) | 2013-10-18 | 2015-11-10 | Abbvie, Inc. | Modulated lysine variant species compositions and methods for producing and using the same |
| WO2015073884A2 (en) | 2013-11-15 | 2015-05-21 | Abbvie, Inc. | Glycoengineered binding protein compositions |
| EP3683316A1 (en) | 2014-01-30 | 2020-07-22 | Coherus Biosciences, Inc. | Perfusion media |
| MX368664B (es) | 2014-04-29 | 2019-10-10 | Novartis Ag | Células novedosas de vertebrados y métodos para la expresión recombinante de un polipéptido de interés. |
| EP2975050B1 (en) | 2014-07-18 | 2019-05-29 | Sandoz Ag | Quantification of misfolded TNFR2:Fc |
| RU2759945C2 (ru) * | 2015-12-17 | 2021-11-19 | Лайф Текнолоджиз Корпорейшн | Гранулы, используемые в клеточной культуре, и способы их получения |
| CA3019753C (en) * | 2016-04-05 | 2021-10-26 | Pfizer Inc. | Cell culture process |
| JP6943972B2 (ja) * | 2017-03-31 | 2021-10-06 | ベーリンガー インゲルハイム インターナショナル ゲゼルシャフト ミット ベシュレンクテル ハフツング | 灌流培地 |
| SG11201908891YA (en) * | 2017-03-31 | 2019-10-30 | Boehringer Ingelheim Int | Perfusion medium |
| AU2020261411B2 (en) | 2019-04-26 | 2025-10-02 | Allogene Therapeutics, Inc. | Methods of manufacturing allogeneic car T cells |
| US12297451B1 (en) | 2019-10-25 | 2025-05-13 | Regeneron Pharmaceuticals, Inc. | Cell culture medium |
| EP4056678A4 (en) * | 2019-11-05 | 2023-12-06 | Ajinomoto Co., Inc. | Method for producing protein |
| IL297191A (en) * | 2020-04-15 | 2022-12-01 | Genentech Inc | A method for treating the medium before inoculation |
| JP2023537760A (ja) * | 2020-08-14 | 2023-09-05 | ブリストル－マイヤーズ スクイブ カンパニー | タンパク質の製造工程 |
| UY39484A (es) | 2020-11-02 | 2022-06-30 | Novartis Ag | Inhibidores de interleucina-17 |
| CN114606200B (zh) * | 2022-02-10 | 2025-07-18 | 上海迈邦生物科技有限公司 | Sp2/0细胞培养基及生产重组犬细小病毒单克隆抗体的方法 |
Family Cites Families (33)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5672502A (en) | 1985-06-28 | 1997-09-30 | Celltech Therapeutics Limited | Animal cell culture |
| EP0283942B1 (en) | 1987-03-24 | 1992-05-20 | W.R. Grace & Co.-Conn. | Basal nutrient medium for cell culture |
| US6048728A (en) * | 1988-09-23 | 2000-04-11 | Chiron Corporation | Cell culture medium for enhanced cell growth, culture longevity, and product expression |
| US5135866A (en) | 1989-03-03 | 1992-08-04 | W. R. Grace & Co.-Conn. | Very low protein nutrient medium for cell culture |
| CA2001550A1 (en) | 1989-03-03 | 1990-09-03 | Aaron H. Heifetz | Very low protein nutrient medium for cell culture |
| US5122469A (en) | 1990-10-03 | 1992-06-16 | Genentech, Inc. | Method for culturing Chinese hamster ovary cells to improve production of recombinant proteins |
| GB9022545D0 (en) | 1990-10-17 | 1990-11-28 | Wellcome Found | Culture medium |
| JPH0789908B2 (ja) | 1991-02-28 | 1995-10-04 | 倉敷紡績株式会社 | 動物細胞培養用無血清培地 |
| GB9118664D0 (en) * | 1991-08-30 | 1991-10-16 | Celltech Ltd | Cell culture |
| US5856179A (en) | 1994-03-10 | 1999-01-05 | Genentech, Inc. | Polypeptide production in animal cell culture |
| US5830761A (en) | 1995-06-07 | 1998-11-03 | Genetics Institute, Inc. | Medium and methods for culturing mammalian cho cells |
| AU4330597A (en) | 1996-08-30 | 1998-03-19 | Life Technologies, Inc. | Serum-free mammalian cell culture medium, and uses thereof |
| US5804420A (en) | 1997-04-18 | 1998-09-08 | Bayer Corporation | Preparation of recombinant Factor VIII in a protein free medium |
| US6406909B1 (en) | 1998-07-10 | 2002-06-18 | Chugai Seiyaku Kabushiki Kaisha | Serum-free medium for culturing animal cells |
| US7294481B1 (en) | 1999-01-05 | 2007-11-13 | Immunex Corporation | Method for producing recombinant proteins |
| EP1757700A3 (en) | 1999-04-26 | 2009-03-11 | Genentech, Inc. | Cell culture process for glycoproteins |
| WO2000073431A2 (en) | 1999-05-27 | 2000-12-07 | Pharmacia & Upjohn Company | Methods and compositions for measuring ion channel conductance |
| AT409379B (de) * | 1999-06-02 | 2002-07-25 | Baxter Ag | Medium zur protein- und serumfreien kultivierung von zellen |
| DK1242578T3 (da) | 1999-12-28 | 2004-07-26 | Iso Tis N V | Celledyrkningsmedium indeholdende vækstfaktorer og L-glutamin |
| ATE420945T1 (de) | 2001-02-15 | 2009-01-15 | Centocor Inc | Chemisch definiertes medium für kultivierte säugerzellen |
| WO2002101019A2 (en) | 2001-06-13 | 2002-12-19 | Genentech, Inc. | Methods of culturing animal cells and polypeptide production in animal cells |
| JP4510938B2 (ja) | 2002-07-15 | 2010-07-28 | イミュネックス・コーポレーション | 哺乳動物細胞により産生されるタンパク質のシアル酸付加を調節する方法および培地 |
| KR20050088136A (ko) | 2002-12-23 | 2005-09-01 | 브리스톨-마이어스 스큅 컴퍼니 | 단백질 생산을 위한 포유류 세포 배양 방법 |
| GB0304799D0 (en) * | 2003-03-03 | 2003-04-09 | Glaxosmithkline Biolog Sa | Novel method |
| DE10338536A1 (de) | 2003-08-19 | 2005-04-07 | Bircher Reglomat Ag | Verfahren zum Betreiben eines Radarsensors |
| US7335491B2 (en) | 2004-08-27 | 2008-02-26 | Wyeth Research Ireland Limited | Production of anti-abeta |
| US7294484B2 (en) * | 2004-08-27 | 2007-11-13 | Wyeth Research Ireland Limited | Production of polypeptides |
| EP1707634A1 (en) | 2005-03-29 | 2006-10-04 | Octapharma AG | Method for isolation of recombinantly produced proteins |
| WO2006113629A1 (en) | 2005-04-15 | 2006-10-26 | The Trustees Of Columbia University In The City Of New York | Chemically defined culture media for expansion and differentiation of epidermal cells and uses thereof for in vitro growth of hair follicles |
| US20070231895A1 (en) | 2005-11-02 | 2007-10-04 | Lee Gene W | Methods for adapting mammalian cells |
| US20070190057A1 (en) | 2006-01-23 | 2007-08-16 | Jian Wu | Methods for modulating mannose content of recombinant proteins |
| PL2078071T3 (pl) * | 2006-11-08 | 2015-07-31 | Wyeth Llc | Racjonalnie opracowane pożywki do hodowli komórek |
| BR112012027282B1 (pt) | 2010-04-26 | 2020-09-29 | Novartis Ag | Meio de cultura de células aperfeiçoado e processo para a produção de um polipeptídeo recombinante |
-
2011
- 2011-04-25 BR BR112012027282-9A patent/BR112012027282B1/pt active IP Right Grant
- 2011-04-25 AU AU2011246503A patent/AU2011246503B2/en active Active
- 2011-04-25 CA CA2797356A patent/CA2797356C/en active Active
- 2011-04-25 KR KR1020127030709A patent/KR101828623B1/ko active Active
- 2011-04-25 DK DK11717240T patent/DK2563904T3/en active
- 2011-04-25 MX MX2012012526A patent/MX2012012526A/es active IP Right Grant
- 2011-04-25 EP EP11717240.3A patent/EP2563904B1/en not_active Revoked
- 2011-04-25 US US16/114,731 patent/USRE48864E1/en active Active
- 2011-04-25 CN CN201180021024.XA patent/CN102858952B/zh active Active
- 2011-04-25 US US13/643,998 patent/US9428727B2/en not_active Ceased
- 2011-04-25 PT PT117172403T patent/PT2563904E/pt unknown
- 2011-04-25 RU RU2012150346A patent/RU2644651C2/ru active
- 2011-04-25 WO PCT/EP2011/056508 patent/WO2011134920A1/en not_active Ceased
- 2011-04-25 SG SG2012079174A patent/SG185038A1/en unknown
- 2011-04-25 PL PL11717240T patent/PL2563904T3/pl unknown
- 2011-04-25 ES ES11717240.3T patent/ES2533074T3/es active Active
- 2011-04-25 JP JP2013506612A patent/JP6347949B2/ja active Active
-
2012
- 2012-10-15 IL IL222452A patent/IL222452B/en active IP Right Grant
-
2016
- 2016-08-03 JP JP2016152747A patent/JP6603628B2/ja active Active
-
2019
- 2019-08-01 JP JP2019142124A patent/JP7034991B2/ja active Active
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