JP2013524825A5 - - Google Patents
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- Publication number
- JP2013524825A5 JP2013524825A5 JP2013506612A JP2013506612A JP2013524825A5 JP 2013524825 A5 JP2013524825 A5 JP 2013524825A5 JP 2013506612 A JP2013506612 A JP 2013506612A JP 2013506612 A JP2013506612 A JP 2013506612A JP 2013524825 A5 JP2013524825 A5 JP 2013524825A5
- Authority
- JP
- Japan
- Prior art keywords
- cell culture
- culture medium
- concentration
- cells
- molar ratio
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 239000006143 cell culture medium Substances 0.000 claims 8
- 238000000034 method Methods 0.000 claims 8
- 229920001184 polypeptide Polymers 0.000 claims 4
- 102000004196 processed proteins & peptides Human genes 0.000 claims 4
- 108090000765 processed proteins & peptides Proteins 0.000 claims 4
- 210000004962 mammalian cell Anatomy 0.000 claims 3
- 229910001414 potassium ion Inorganic materials 0.000 claims 3
- 229910001415 sodium ion Inorganic materials 0.000 claims 3
- WCUXLLCKKVVCTQ-UHFFFAOYSA-M Potassium chloride Chemical compound [Cl-].[K+] WCUXLLCKKVVCTQ-UHFFFAOYSA-M 0.000 claims 2
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims 2
- 150000001413 amino acids Chemical class 0.000 claims 2
- 210000004027 cell Anatomy 0.000 claims 2
- 239000002609 medium Substances 0.000 claims 2
- 102000008394 Immunoglobulin Fragments Human genes 0.000 claims 1
- 108010021625 Immunoglobulin Fragments Proteins 0.000 claims 1
- NPYPAHLBTDXSSS-UHFFFAOYSA-N Potassium ion Chemical compound [K+] NPYPAHLBTDXSSS-UHFFFAOYSA-N 0.000 claims 1
- FKNQFGJONOIPTF-UHFFFAOYSA-N Sodium cation Chemical compound [Na+] FKNQFGJONOIPTF-UHFFFAOYSA-N 0.000 claims 1
- 238000010923 batch production Methods 0.000 claims 1
- 210000004978 chinese hamster ovary cell Anatomy 0.000 claims 1
- 238000012258 culturing Methods 0.000 claims 1
- 150000002500 ions Chemical class 0.000 claims 1
- 238000004519 manufacturing process Methods 0.000 claims 1
- 239000001103 potassium chloride Substances 0.000 claims 1
- 235000011164 potassium chloride Nutrition 0.000 claims 1
- 239000011780 sodium chloride Substances 0.000 claims 1
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US32783610P | 2010-04-26 | 2010-04-26 | |
| US61/327,836 | 2010-04-26 | ||
| PCT/EP2011/056508 WO2011134920A1 (en) | 2010-04-26 | 2011-04-25 | Improved cell culture medium |
Related Child Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2016152747A Division JP6603628B2 (ja) | 2010-04-26 | 2016-08-03 | 改良型細胞培養培地 |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2013524825A JP2013524825A (ja) | 2013-06-20 |
| JP2013524825A5 true JP2013524825A5 (enExample) | 2014-06-05 |
| JP6347949B2 JP6347949B2 (ja) | 2018-06-27 |
Family
ID=44074260
Family Applications (3)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2013506612A Active JP6347949B2 (ja) | 2010-04-26 | 2011-04-25 | 改良型細胞培養培地 |
| JP2016152747A Active JP6603628B2 (ja) | 2010-04-26 | 2016-08-03 | 改良型細胞培養培地 |
| JP2019142124A Active JP7034991B2 (ja) | 2010-04-26 | 2019-08-01 | 改良型細胞培養培地 |
Family Applications After (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2016152747A Active JP6603628B2 (ja) | 2010-04-26 | 2016-08-03 | 改良型細胞培養培地 |
| JP2019142124A Active JP7034991B2 (ja) | 2010-04-26 | 2019-08-01 | 改良型細胞培養培地 |
Country Status (17)
| Country | Link |
|---|---|
| US (2) | USRE48864E1 (enExample) |
| EP (1) | EP2563904B1 (enExample) |
| JP (3) | JP6347949B2 (enExample) |
| KR (1) | KR101828623B1 (enExample) |
| CN (1) | CN102858952B (enExample) |
| AU (1) | AU2011246503B2 (enExample) |
| BR (1) | BR112012027282B1 (enExample) |
| CA (1) | CA2797356C (enExample) |
| DK (1) | DK2563904T3 (enExample) |
| ES (1) | ES2533074T3 (enExample) |
| IL (1) | IL222452B (enExample) |
| MX (1) | MX2012012526A (enExample) |
| PL (1) | PL2563904T3 (enExample) |
| PT (1) | PT2563904E (enExample) |
| RU (1) | RU2644651C2 (enExample) |
| SG (1) | SG185038A1 (enExample) |
| WO (1) | WO2011134920A1 (enExample) |
Families Citing this family (48)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US8911964B2 (en) | 2006-09-13 | 2014-12-16 | Abbvie Inc. | Fed-batch method of making human anti-TNF-alpha antibody |
| TW201516149A (zh) | 2006-09-13 | 2015-05-01 | Abbvie Inc | 細胞培養改良 |
| CA2932207A1 (en) | 2008-10-20 | 2010-12-09 | Abbvie Inc. | Isolation and purification of antibodies using protein a affinity chromatography |
| CN104974251A (zh) | 2008-10-20 | 2015-10-14 | Abbvie公司 | 在抗体纯化过程中的病毒灭活 |
| CN102858953B (zh) | 2010-04-26 | 2015-09-09 | 诺瓦提斯公司 | 改进的细胞培养基 |
| AU2011246503B2 (en) | 2010-04-26 | 2013-09-26 | Novartis Ag | Improved cell culture medium |
| BR112013009934A2 (pt) * | 2010-12-07 | 2016-07-05 | Hoffmann La Roche | dispositivo para adicionar pelo menos duas soluções de alimentação, aparelho de cultivo celular, uso de um dispositivo e método para obter um polipeptídeo |
| WO2012149197A2 (en) | 2011-04-27 | 2012-11-01 | Abbott Laboratories | Methods for controlling the galactosylation profile of recombinantly-expressed proteins |
| AU2012326170B2 (en) | 2011-10-18 | 2016-12-22 | Coherus Biosciences, Inc. | Etanercept formulations stabilized with metal ions |
| US10485869B2 (en) | 2011-10-18 | 2019-11-26 | Coherus Biosciences, Inc. | Etanercept formulations stabilized with meglumine |
| US9150645B2 (en) | 2012-04-20 | 2015-10-06 | Abbvie, Inc. | Cell culture methods to reduce acidic species |
| US9181572B2 (en) | 2012-04-20 | 2015-11-10 | Abbvie, Inc. | Methods to modulate lysine variant distribution |
| US9067990B2 (en) | 2013-03-14 | 2015-06-30 | Abbvie, Inc. | Protein purification using displacement chromatography |
| US20130281355A1 (en) * | 2012-04-24 | 2013-10-24 | Genentech, Inc. | Cell culture compositions and methods for polypeptide production |
| US9249182B2 (en) | 2012-05-24 | 2016-02-02 | Abbvie, Inc. | Purification of antibodies using hydrophobic interaction chromatography |
| WO2014011629A1 (en) | 2012-07-09 | 2014-01-16 | Coherus Biosciences, Inc. | Stable aqueous formulations of etanercept |
| HK1211981A1 (en) | 2012-09-02 | 2016-06-03 | Abbvie Inc. | Methods to control protein heterogeneity |
| US9512214B2 (en) | 2012-09-02 | 2016-12-06 | Abbvie, Inc. | Methods to control protein heterogeneity |
| KR102250937B1 (ko) | 2012-09-11 | 2021-05-12 | 코히러스 바이오사이언시즈, 인코포레이티드 | 고순도 및 탁월한 수율의 정확하게 폴딩된 에타너셉트 |
| SG11201507230PA (en) | 2013-03-12 | 2015-10-29 | Abbvie Inc | Human antibodies that bind human tnf-alpha and methods of preparing the same |
| WO2014159579A1 (en) | 2013-03-14 | 2014-10-02 | Abbvie Inc. | MUTATED ANTI-TNFα ANTIBODIES AND METHODS OF THEIR USE |
| BR112015017307A2 (pt) | 2013-03-14 | 2017-11-21 | Abbvie Inc | composições de espécies com baixa acidez e métodos para produção e uso das mesmas |
| US9017687B1 (en) | 2013-10-18 | 2015-04-28 | Abbvie, Inc. | Low acidic species compositions and methods for producing and using the same using displacement chromatography |
| EP3036254A1 (en) | 2013-08-20 | 2016-06-29 | LEK Pharmaceuticals d.d. | Cell culture medium and process for controlling -amidation and/or c-terminal amino acid cleavage of polypeptides |
| US9598667B2 (en) | 2013-10-04 | 2017-03-21 | Abbvie Inc. | Use of metal ions for modulation of protein glycosylation profiles of recombinant proteins |
| US11390663B2 (en) | 2013-10-11 | 2022-07-19 | Regeneron Pharmaceuticals, Inc. | Metabolically optimized cell culture |
| PL3812453T3 (pl) * | 2013-10-11 | 2026-07-13 | Regeneron Pharmaceuticals, Inc. | Hodowla komórkowa zoptymalizowana metabolicznie |
| US8946395B1 (en) | 2013-10-18 | 2015-02-03 | Abbvie Inc. | Purification of proteins using hydrophobic interaction chromatography |
| US9085618B2 (en) | 2013-10-18 | 2015-07-21 | Abbvie, Inc. | Low acidic species compositions and methods for producing and using the same |
| US9181337B2 (en) | 2013-10-18 | 2015-11-10 | Abbvie, Inc. | Modulated lysine variant species compositions and methods for producing and using the same |
| US20150139988A1 (en) | 2013-11-15 | 2015-05-21 | Abbvie, Inc. | Glycoengineered binding protein compositions |
| US10047141B2 (en) * | 2014-01-30 | 2018-08-14 | Coherus Biosciences, Inc. | Method of manufacturing a protein by perfusion in media with a low amino acid concentration |
| SG11201608358VA (en) | 2014-04-29 | 2016-11-29 | Novartis Ag | Novel vertebrate cells and methods for recombinantly expressing a polypeptide of interest |
| EP2975050B1 (en) | 2014-07-18 | 2019-05-29 | Sandoz Ag | Quantification of misfolded TNFR2:Fc |
| US10822584B2 (en) * | 2015-12-17 | 2020-11-03 | Life Technologies Corporation | Pellets used in cell culture and methods of making thereof |
| EP3440190B1 (en) * | 2016-04-05 | 2024-08-28 | Pfizer Inc. | Cell culture process |
| CN110462054B (zh) * | 2017-03-31 | 2023-11-10 | 勃林格殷格翰国际公司 | 灌注培养基 |
| WO2018178069A1 (en) | 2017-03-31 | 2018-10-04 | Boehringer Ingelheim International Gmbh | Perfusion medium |
| EP3959305A1 (en) | 2019-04-26 | 2022-03-02 | Allogene Therapeutics, Inc. | Methods of manufacturing allogeneic car t cells |
| US12297451B1 (en) | 2019-10-25 | 2025-05-13 | Regeneron Pharmaceuticals, Inc. | Cell culture medium |
| WO2021090888A1 (ja) * | 2019-11-05 | 2021-05-14 | 味の素株式会社 | タンパク質の製造方法 |
| AU2021257609A1 (en) * | 2020-04-15 | 2022-11-10 | F. Hoffmann-La Roche Ag | Method for medium treatment before inoculation |
| EP4196597A2 (en) * | 2020-08-14 | 2023-06-21 | Bristol-Myers Squibb Company | Manufacturing process for protein |
| UY39484A (es) | 2020-11-02 | 2022-06-30 | Novartis Ag | Inhibidores de interleucina-17 |
| CN114606200B (zh) * | 2022-02-10 | 2025-07-18 | 上海迈邦生物科技有限公司 | Sp2/0细胞培养基及生产重组犬细小病毒单克隆抗体的方法 |
| WO2024218715A1 (en) | 2023-04-19 | 2024-10-24 | Btl Healthcare Technologies A.S. | Culture media based on protein hydrolysate and a process for preparing thereof |
| US12668774B2 (en) | 2023-10-12 | 2026-06-30 | Btl Healthcare Technologies A.S. | Product, system and method of cell cultivation |
| WO2026009803A1 (ja) * | 2024-07-05 | 2026-01-08 | 国立大学法人九州大学 | ヘパリン様物質を製造する方法 |
Family Cites Families (33)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5672502A (en) | 1985-06-28 | 1997-09-30 | Celltech Therapeutics Limited | Animal cell culture |
| DE3871206D1 (de) | 1987-03-24 | 1992-06-25 | Grace W R & Co | Basisnaehrmedium fuer eine zellkultur. |
| US6048728A (en) * | 1988-09-23 | 2000-04-11 | Chiron Corporation | Cell culture medium for enhanced cell growth, culture longevity, and product expression |
| CA2001550A1 (en) | 1989-03-03 | 1990-09-03 | Aaron H. Heifetz | Very low protein nutrient medium for cell culture |
| US5135866A (en) | 1989-03-03 | 1992-08-04 | W. R. Grace & Co.-Conn. | Very low protein nutrient medium for cell culture |
| US5122469A (en) | 1990-10-03 | 1992-06-16 | Genentech, Inc. | Method for culturing Chinese hamster ovary cells to improve production of recombinant proteins |
| GB9022545D0 (en) | 1990-10-17 | 1990-11-28 | Wellcome Found | Culture medium |
| JPH0789908B2 (ja) | 1991-02-28 | 1995-10-04 | 倉敷紡績株式会社 | 動物細胞培養用無血清培地 |
| GB9118664D0 (en) * | 1991-08-30 | 1991-10-16 | Celltech Ltd | Cell culture |
| US5856179A (en) | 1994-03-10 | 1999-01-05 | Genentech, Inc. | Polypeptide production in animal cell culture |
| US5830761A (en) | 1995-06-07 | 1998-11-03 | Genetics Institute, Inc. | Medium and methods for culturing mammalian cho cells |
| EP2221361A3 (en) * | 1996-08-30 | 2011-02-09 | Life Technologies Corporation | Method for producing a polypeptide in vitro in mammalian cells in a protein-free and serum-free culture medium |
| US5804420A (en) | 1997-04-18 | 1998-09-08 | Bayer Corporation | Preparation of recombinant Factor VIII in a protein free medium |
| US6406909B1 (en) | 1998-07-10 | 2002-06-18 | Chugai Seiyaku Kabushiki Kaisha | Serum-free medium for culturing animal cells |
| US7294481B1 (en) | 1999-01-05 | 2007-11-13 | Immunex Corporation | Method for producing recombinant proteins |
| EP1757700A3 (en) | 1999-04-26 | 2009-03-11 | Genentech, Inc. | Cell culture process for glycoproteins |
| WO2000073431A2 (en) | 1999-05-27 | 2000-12-07 | Pharmacia & Upjohn Company | Methods and compositions for measuring ion channel conductance |
| AT409379B (de) * | 1999-06-02 | 2002-07-25 | Baxter Ag | Medium zur protein- und serumfreien kultivierung von zellen |
| DE60009956T2 (de) | 1999-12-28 | 2005-04-28 | Iso Tis N.V. | Wachstumsfaktoren und l-glutamin enthaltendes zellkulturmedium |
| DE60230858D1 (de) | 2001-02-15 | 2009-03-05 | Centocor Inc | Chemisch definiertes medium für kultivierte säugerzellen |
| US20030087372A1 (en) | 2001-06-13 | 2003-05-08 | Genentech, Inc. | Methods of culturing animal cells and polypeptide production in animal cells |
| CA2492267C (en) | 2002-07-15 | 2013-09-10 | Immunex Corporation | Methods and media for controlling sialylation of proteins produced by mammalian cells |
| EP1575998A4 (en) | 2002-12-23 | 2007-07-25 | Bristol Myers Squibb Co | PROCESS FOR CULTIVATING MAMMALIAN CELLS FOR PROTEIN PRODUCTION |
| GB0304799D0 (en) | 2003-03-03 | 2003-04-09 | Glaxosmithkline Biolog Sa | Novel method |
| DE10338536A1 (de) | 2003-08-19 | 2005-04-07 | Bircher Reglomat Ag | Verfahren zum Betreiben eines Radarsensors |
| US7294484B2 (en) * | 2004-08-27 | 2007-11-13 | Wyeth Research Ireland Limited | Production of polypeptides |
| US7335491B2 (en) | 2004-08-27 | 2008-02-26 | Wyeth Research Ireland Limited | Production of anti-abeta |
| EP1707634A1 (en) | 2005-03-29 | 2006-10-04 | Octapharma AG | Method for isolation of recombinantly produced proteins |
| EP1937798A4 (en) | 2005-04-15 | 2009-05-13 | Univ Columbia | CHEMICALLY DEFINED CULTURAL MEDIA FOR THE EXPANSION AND DIFFERENTIATION OF EPIDERMIC CELLS AND USES THEREOF FOR THE IN VITRO FARMING OF HAIRFOLLICLES |
| US20070231895A1 (en) | 2005-11-02 | 2007-10-04 | Lee Gene W | Methods for adapting mammalian cells |
| US20070190057A1 (en) | 2006-01-23 | 2007-08-16 | Jian Wu | Methods for modulating mannose content of recombinant proteins |
| MX2009004974A (es) * | 2006-11-08 | 2009-05-20 | Wyeth Corp | Medios diseñado racionalmente para cultivo celular. |
| AU2011246503B2 (en) * | 2010-04-26 | 2013-09-26 | Novartis Ag | Improved cell culture medium |
-
2011
- 2011-04-25 AU AU2011246503A patent/AU2011246503B2/en active Active
- 2011-04-25 SG SG2012079174A patent/SG185038A1/en unknown
- 2011-04-25 US US16/114,731 patent/USRE48864E1/en active Active
- 2011-04-25 CA CA2797356A patent/CA2797356C/en active Active
- 2011-04-25 US US13/643,998 patent/US9428727B2/en not_active Ceased
- 2011-04-25 PL PL11717240T patent/PL2563904T3/pl unknown
- 2011-04-25 JP JP2013506612A patent/JP6347949B2/ja active Active
- 2011-04-25 CN CN201180021024.XA patent/CN102858952B/zh active Active
- 2011-04-25 PT PT117172403T patent/PT2563904E/pt unknown
- 2011-04-25 WO PCT/EP2011/056508 patent/WO2011134920A1/en not_active Ceased
- 2011-04-25 RU RU2012150346A patent/RU2644651C2/ru active
- 2011-04-25 EP EP11717240.3A patent/EP2563904B1/en not_active Revoked
- 2011-04-25 MX MX2012012526A patent/MX2012012526A/es active IP Right Grant
- 2011-04-25 KR KR1020127030709A patent/KR101828623B1/ko active Active
- 2011-04-25 BR BR112012027282-9A patent/BR112012027282B1/pt active IP Right Grant
- 2011-04-25 DK DK11717240T patent/DK2563904T3/en active
- 2011-04-25 ES ES11717240.3T patent/ES2533074T3/es active Active
-
2012
- 2012-10-15 IL IL222452A patent/IL222452B/en active IP Right Grant
-
2016
- 2016-08-03 JP JP2016152747A patent/JP6603628B2/ja active Active
-
2019
- 2019-08-01 JP JP2019142124A patent/JP7034991B2/ja active Active
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