JP2013524825A5 - - Google Patents
Download PDFInfo
- Publication number
- JP2013524825A5 JP2013524825A5 JP2013506612A JP2013506612A JP2013524825A5 JP 2013524825 A5 JP2013524825 A5 JP 2013524825A5 JP 2013506612 A JP2013506612 A JP 2013506612A JP 2013506612 A JP2013506612 A JP 2013506612A JP 2013524825 A5 JP2013524825 A5 JP 2013524825A5
- Authority
- JP
- Japan
- Prior art keywords
- cell culture
- culture medium
- concentration
- cells
- molar ratio
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 239000006143 cell culture medium Substances 0.000 claims 8
- 238000000034 method Methods 0.000 claims 8
- 229920001184 polypeptide Polymers 0.000 claims 4
- 102000004196 processed proteins & peptides Human genes 0.000 claims 4
- 108090000765 processed proteins & peptides Proteins 0.000 claims 4
- 210000004962 mammalian cell Anatomy 0.000 claims 3
- 229910001414 potassium ion Inorganic materials 0.000 claims 3
- 229910001415 sodium ion Inorganic materials 0.000 claims 3
- WCUXLLCKKVVCTQ-UHFFFAOYSA-M Potassium chloride Chemical compound [Cl-].[K+] WCUXLLCKKVVCTQ-UHFFFAOYSA-M 0.000 claims 2
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims 2
- 150000001413 amino acids Chemical class 0.000 claims 2
- 210000004027 cell Anatomy 0.000 claims 2
- 239000002609 medium Substances 0.000 claims 2
- 102000008394 Immunoglobulin Fragments Human genes 0.000 claims 1
- 108010021625 Immunoglobulin Fragments Proteins 0.000 claims 1
- NPYPAHLBTDXSSS-UHFFFAOYSA-N Potassium ion Chemical compound [K+] NPYPAHLBTDXSSS-UHFFFAOYSA-N 0.000 claims 1
- FKNQFGJONOIPTF-UHFFFAOYSA-N Sodium cation Chemical compound [Na+] FKNQFGJONOIPTF-UHFFFAOYSA-N 0.000 claims 1
- 238000010923 batch production Methods 0.000 claims 1
- 210000004978 chinese hamster ovary cell Anatomy 0.000 claims 1
- 238000012258 culturing Methods 0.000 claims 1
- 150000002500 ions Chemical class 0.000 claims 1
- 238000004519 manufacturing process Methods 0.000 claims 1
- 239000001103 potassium chloride Substances 0.000 claims 1
- 235000011164 potassium chloride Nutrition 0.000 claims 1
- 239000011780 sodium chloride Substances 0.000 claims 1
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US32783610P | 2010-04-26 | 2010-04-26 | |
| US61/327,836 | 2010-04-26 | ||
| PCT/EP2011/056508 WO2011134920A1 (en) | 2010-04-26 | 2011-04-25 | Improved cell culture medium |
Related Child Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2016152747A Division JP6603628B2 (ja) | 2010-04-26 | 2016-08-03 | 改良型細胞培養培地 |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2013524825A JP2013524825A (ja) | 2013-06-20 |
| JP2013524825A5 true JP2013524825A5 (enExample) | 2014-06-05 |
| JP6347949B2 JP6347949B2 (ja) | 2018-06-27 |
Family
ID=44074260
Family Applications (3)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2013506612A Active JP6347949B2 (ja) | 2010-04-26 | 2011-04-25 | 改良型細胞培養培地 |
| JP2016152747A Active JP6603628B2 (ja) | 2010-04-26 | 2016-08-03 | 改良型細胞培養培地 |
| JP2019142124A Active JP7034991B2 (ja) | 2010-04-26 | 2019-08-01 | 改良型細胞培養培地 |
Family Applications After (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2016152747A Active JP6603628B2 (ja) | 2010-04-26 | 2016-08-03 | 改良型細胞培養培地 |
| JP2019142124A Active JP7034991B2 (ja) | 2010-04-26 | 2019-08-01 | 改良型細胞培養培地 |
Country Status (17)
| Country | Link |
|---|---|
| US (2) | USRE48864E1 (enExample) |
| EP (1) | EP2563904B1 (enExample) |
| JP (3) | JP6347949B2 (enExample) |
| KR (1) | KR101828623B1 (enExample) |
| CN (1) | CN102858952B (enExample) |
| AU (1) | AU2011246503B2 (enExample) |
| BR (1) | BR112012027282B1 (enExample) |
| CA (1) | CA2797356C (enExample) |
| DK (1) | DK2563904T3 (enExample) |
| ES (1) | ES2533074T3 (enExample) |
| IL (1) | IL222452B (enExample) |
| MX (1) | MX2012012526A (enExample) |
| PL (1) | PL2563904T3 (enExample) |
| PT (1) | PT2563904E (enExample) |
| RU (1) | RU2644651C2 (enExample) |
| SG (1) | SG185038A1 (enExample) |
| WO (1) | WO2011134920A1 (enExample) |
Families Citing this family (46)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| MX353340B (es) | 2006-09-13 | 2018-01-09 | Abbvie Inc | Mejoras de cultivos celulares. |
| US8911964B2 (en) | 2006-09-13 | 2014-12-16 | Abbvie Inc. | Fed-batch method of making human anti-TNF-alpha antibody |
| TW201024318A (en) | 2008-10-20 | 2010-07-01 | Abbott Lab | Isolation and purification of antibodies using protein A affinity chromatography |
| SG195577A1 (en) | 2008-10-20 | 2013-12-30 | Abbott Lab | Viral inactivation during purification of antibodies |
| PT2563904E (pt) * | 2010-04-26 | 2015-05-12 | Novartis Ag | Meio de cultura de células aperfeiçoado |
| BR112012027434B1 (pt) | 2010-04-26 | 2020-09-29 | Novartis Ag | Processo para produção de um polipeptídeo recombinante compreendendo uma fase de produção |
| CN103221534B (zh) * | 2010-12-07 | 2016-05-18 | 弗·哈夫曼-拉罗切有限公司 | 进料混合装置及其用途 |
| US9062106B2 (en) | 2011-04-27 | 2015-06-23 | Abbvie Inc. | Methods for controlling the galactosylation profile of recombinantly-expressed proteins |
| SG11201401562RA (en) | 2011-10-18 | 2014-09-26 | Coherus Biosciences Inc | Etanercept formulations stabilized with sodium chloride |
| US10493151B2 (en) | 2011-10-18 | 2019-12-03 | Coherus Biosciences, Inc. | Etanercept formulations stabilized with sodium chloride |
| US9067990B2 (en) | 2013-03-14 | 2015-06-30 | Abbvie, Inc. | Protein purification using displacement chromatography |
| WO2013158273A1 (en) | 2012-04-20 | 2013-10-24 | Abbvie Inc. | Methods to modulate c-terminal lysine variant distribution |
| WO2013158279A1 (en) | 2012-04-20 | 2013-10-24 | Abbvie Inc. | Protein purification methods to reduce acidic species |
| US20130281355A1 (en) * | 2012-04-24 | 2013-10-24 | Genentech, Inc. | Cell culture compositions and methods for polypeptide production |
| US9249182B2 (en) | 2012-05-24 | 2016-02-02 | Abbvie, Inc. | Purification of antibodies using hydrophobic interaction chromatography |
| JP6271536B2 (ja) | 2012-07-09 | 2018-01-31 | コヒラス・バイオサイエンシズ・インコーポレイテッド | 肉眼不可視粒子の著しい低減を示すエタネルセプト製剤 |
| US9512214B2 (en) | 2012-09-02 | 2016-12-06 | Abbvie, Inc. | Methods to control protein heterogeneity |
| KR20150043523A (ko) | 2012-09-02 | 2015-04-22 | 애브비 인코포레이티드 | 단백질 불균일성의 제어 방법 |
| AU2013315750B9 (en) | 2012-09-11 | 2018-11-15 | Coherus Biosciences, Inc. | Correctly folded etanercept in high purity and excellent yield |
| SG11201507230PA (en) | 2013-03-12 | 2015-10-29 | Abbvie Inc | Human antibodies that bind human tnf-alpha and methods of preparing the same |
| US9017687B1 (en) | 2013-10-18 | 2015-04-28 | Abbvie, Inc. | Low acidic species compositions and methods for producing and using the same using displacement chromatography |
| KR20150129033A (ko) | 2013-03-14 | 2015-11-18 | 애브비 인코포레이티드 | 저 산성 종 조성물 및 이의 제조 및 사용 방법 |
| US8921526B2 (en) | 2013-03-14 | 2014-12-30 | Abbvie, Inc. | Mutated anti-TNFα antibodies and methods of their use |
| US10196601B2 (en) | 2013-08-20 | 2019-02-05 | Lek Pharmaceuticals D.D. | Cell culture medium and process for controlling α-amidation and/or C-terminal amino acid cleavage of polypeptides |
| EP3052640A2 (en) | 2013-10-04 | 2016-08-10 | AbbVie Inc. | Use of metal ions for modulation of protein glycosylation profiles of recombinant proteins |
| DK3351620T3 (da) * | 2013-10-11 | 2020-10-26 | Regeneron Pharma | Metabolsk optimeret cellekultur |
| US11390663B2 (en) | 2013-10-11 | 2022-07-19 | Regeneron Pharmaceuticals, Inc. | Metabolically optimized cell culture |
| US8946395B1 (en) | 2013-10-18 | 2015-02-03 | Abbvie Inc. | Purification of proteins using hydrophobic interaction chromatography |
| US9181337B2 (en) | 2013-10-18 | 2015-11-10 | Abbvie, Inc. | Modulated lysine variant species compositions and methods for producing and using the same |
| US9085618B2 (en) | 2013-10-18 | 2015-07-21 | Abbvie, Inc. | Low acidic species compositions and methods for producing and using the same |
| WO2015073884A2 (en) | 2013-11-15 | 2015-05-21 | Abbvie, Inc. | Glycoengineered binding protein compositions |
| WO2015116820A1 (en) | 2014-01-30 | 2015-08-06 | Coherus Biosciences, Inc. | Perfusion media |
| EP4332231A3 (en) | 2014-04-29 | 2024-05-29 | Novartis AG | Novel vertebrate cells and methods for recombinantly expressing a polypeptide of interest |
| ES2733298T3 (es) | 2014-07-18 | 2019-11-28 | Sandoz Ag | Cuantificación de TNFR2:Fc plegado erróneamente |
| CN108495926A (zh) * | 2015-12-17 | 2018-09-04 | 生命技术公司 | 用于细胞培养的颗粒及其制备方法 |
| CA3019753C (en) * | 2016-04-05 | 2021-10-26 | Pfizer Inc. | Cell culture process |
| KR102604992B1 (ko) * | 2017-03-31 | 2023-11-24 | 베링거 인겔하임 인터내셔날 게엠베하 | 관류 배지 |
| SG11201909087PA (en) * | 2017-03-31 | 2019-10-30 | Boehringer Ingelheim Int | Perfusion medium |
| BR112021021349A2 (pt) | 2019-04-26 | 2022-02-01 | Allogene Therapeutics Inc | Métodos de fabricação de células car t alogênicas |
| US12297451B1 (en) | 2019-10-25 | 2025-05-13 | Regeneron Pharmaceuticals, Inc. | Cell culture medium |
| KR20220097421A (ko) * | 2019-11-05 | 2022-07-07 | 아지노모토 가부시키가이샤 | 단백질의 제조 방법 |
| KR20230002642A (ko) * | 2020-04-15 | 2023-01-05 | 제넨테크, 인크. | 접종 전 배지를 처리하는 방법 |
| US20230313259A1 (en) * | 2020-08-14 | 2023-10-05 | Bristol-Myers Squibb Company | Manufacturing process for protein |
| UY39484A (es) | 2020-11-02 | 2022-06-30 | Novartis Ag | Inhibidores de interleucina-17 |
| CN114606200B (zh) * | 2022-02-10 | 2025-07-18 | 上海迈邦生物科技有限公司 | Sp2/0细胞培养基及生产重组犬细小病毒单克隆抗体的方法 |
| WO2026009803A1 (ja) * | 2024-07-05 | 2026-01-08 | 国立大学法人九州大学 | ヘパリン様物質を製造する方法 |
Family Cites Families (33)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5672502A (en) | 1985-06-28 | 1997-09-30 | Celltech Therapeutics Limited | Animal cell culture |
| DE3871206D1 (de) | 1987-03-24 | 1992-06-25 | Grace W R & Co | Basisnaehrmedium fuer eine zellkultur. |
| US6048728A (en) * | 1988-09-23 | 2000-04-11 | Chiron Corporation | Cell culture medium for enhanced cell growth, culture longevity, and product expression |
| US5135866A (en) | 1989-03-03 | 1992-08-04 | W. R. Grace & Co.-Conn. | Very low protein nutrient medium for cell culture |
| CA2001550A1 (en) | 1989-03-03 | 1990-09-03 | Aaron H. Heifetz | Very low protein nutrient medium for cell culture |
| US5122469A (en) | 1990-10-03 | 1992-06-16 | Genentech, Inc. | Method for culturing Chinese hamster ovary cells to improve production of recombinant proteins |
| GB9022545D0 (en) | 1990-10-17 | 1990-11-28 | Wellcome Found | Culture medium |
| JPH0789908B2 (ja) | 1991-02-28 | 1995-10-04 | 倉敷紡績株式会社 | 動物細胞培養用無血清培地 |
| GB9118664D0 (en) | 1991-08-30 | 1991-10-16 | Celltech Ltd | Cell culture |
| US5856179A (en) | 1994-03-10 | 1999-01-05 | Genentech, Inc. | Polypeptide production in animal cell culture |
| US5830761A (en) | 1995-06-07 | 1998-11-03 | Genetics Institute, Inc. | Medium and methods for culturing mammalian cho cells |
| EP2243827B2 (en) * | 1996-08-30 | 2017-11-22 | Life Technologies Corporation | Serum-free mammalian cell culture medium, and uses thereof |
| US5804420A (en) | 1997-04-18 | 1998-09-08 | Bayer Corporation | Preparation of recombinant Factor VIII in a protein free medium |
| US6406909B1 (en) | 1998-07-10 | 2002-06-18 | Chugai Seiyaku Kabushiki Kaisha | Serum-free medium for culturing animal cells |
| US7294481B1 (en) | 1999-01-05 | 2007-11-13 | Immunex Corporation | Method for producing recombinant proteins |
| EP1757700A3 (en) | 1999-04-26 | 2009-03-11 | Genentech, Inc. | Cell culture process for glycoproteins |
| AU4980200A (en) | 1999-05-27 | 2000-12-18 | Pharmacia & Upjohn Company | Methods and compositions for measuring ion channel conductance |
| AT409379B (de) * | 1999-06-02 | 2002-07-25 | Baxter Ag | Medium zur protein- und serumfreien kultivierung von zellen |
| WO2001048147A1 (en) | 1999-12-28 | 2001-07-05 | Iso Tis N.V. | Cell culture medium containing growth factors and l-glutamine |
| CA2438148A1 (en) | 2001-02-15 | 2002-08-29 | Centocor, Inc. | Chemically defined medium for cultured mammalian cells |
| WO2002101019A2 (en) | 2001-06-13 | 2002-12-19 | Genentech, Inc. | Methods of culturing animal cells and polypeptide production in animal cells |
| WO2004008100A2 (en) | 2002-07-15 | 2004-01-22 | Immunex Corporation | Methods and media for controlling sialylation of proteins produced by mammalian cells |
| AU2003300276B2 (en) | 2002-12-23 | 2010-07-01 | Bristol-Myers Squibb Company | Mammalian cell culture processes for protein production |
| GB0304799D0 (en) * | 2003-03-03 | 2003-04-09 | Glaxosmithkline Biolog Sa | Novel method |
| DE10338536A1 (de) | 2003-08-19 | 2005-04-07 | Bircher Reglomat Ag | Verfahren zum Betreiben eines Radarsensors |
| TWI374935B (en) | 2004-08-27 | 2012-10-21 | Pfizer Ireland Pharmaceuticals | Production of α-abeta |
| TWI384069B (zh) * | 2004-08-27 | 2013-02-01 | Pfizer Ireland Pharmaceuticals | 多胜肽之製法 |
| EP1707634A1 (en) | 2005-03-29 | 2006-10-04 | Octapharma AG | Method for isolation of recombinantly produced proteins |
| EP1937798A4 (en) | 2005-04-15 | 2009-05-13 | Univ Columbia | CHEMICALLY DEFINED CULTURAL MEDIA FOR THE EXPANSION AND DIFFERENTIATION OF EPIDERMIC CELLS AND USES THEREOF FOR THE IN VITRO FARMING OF HAIRFOLLICLES |
| US20070231895A1 (en) | 2005-11-02 | 2007-10-04 | Lee Gene W | Methods for adapting mammalian cells |
| US20070190057A1 (en) * | 2006-01-23 | 2007-08-16 | Jian Wu | Methods for modulating mannose content of recombinant proteins |
| CA2668771C (en) * | 2006-11-08 | 2016-03-15 | Wyeth | Rationally designed media for cell culture |
| PT2563904E (pt) * | 2010-04-26 | 2015-05-12 | Novartis Ag | Meio de cultura de células aperfeiçoado |
-
2011
- 2011-04-25 PT PT117172403T patent/PT2563904E/pt unknown
- 2011-04-25 BR BR112012027282-9A patent/BR112012027282B1/pt active IP Right Grant
- 2011-04-25 ES ES11717240.3T patent/ES2533074T3/es active Active
- 2011-04-25 US US16/114,731 patent/USRE48864E1/en active Active
- 2011-04-25 US US13/643,998 patent/US9428727B2/en not_active Ceased
- 2011-04-25 EP EP11717240.3A patent/EP2563904B1/en not_active Revoked
- 2011-04-25 AU AU2011246503A patent/AU2011246503B2/en active Active
- 2011-04-25 CN CN201180021024.XA patent/CN102858952B/zh active Active
- 2011-04-25 JP JP2013506612A patent/JP6347949B2/ja active Active
- 2011-04-25 DK DK11717240T patent/DK2563904T3/en active
- 2011-04-25 CA CA2797356A patent/CA2797356C/en active Active
- 2011-04-25 WO PCT/EP2011/056508 patent/WO2011134920A1/en not_active Ceased
- 2011-04-25 RU RU2012150346A patent/RU2644651C2/ru active
- 2011-04-25 SG SG2012079174A patent/SG185038A1/en unknown
- 2011-04-25 MX MX2012012526A patent/MX2012012526A/es active IP Right Grant
- 2011-04-25 KR KR1020127030709A patent/KR101828623B1/ko active Active
- 2011-04-25 PL PL11717240T patent/PL2563904T3/pl unknown
-
2012
- 2012-10-15 IL IL222452A patent/IL222452B/en active IP Right Grant
-
2016
- 2016-08-03 JP JP2016152747A patent/JP6603628B2/ja active Active
-
2019
- 2019-08-01 JP JP2019142124A patent/JP7034991B2/ja active Active
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CA2797356A1 (en) | Improved cell culture medium | |
| AR124871A2 (es) | Métodos de fabricación para el control del contenido de lisina c-terminal, galactosa y ácido siálico en proteínas recombinantes | |
| RU2013152982A (ru) | Способ снижения гетерогенности антител и способ получения соответствующих антител | |
| BR112021025359A2 (pt) | Composições de proteína anti-vegf e métodos para a produção das mesmas | |
| SG10201901417UA (en) | Production of proteins in glutamine-free cell culture media | |
| DK2152872T3 (da) | Fremgangsmåde til fremstilling af et rekombinant polyklonalt protein | |
| IL310186B1 (en) | Plasma kallikrein binding proteins | |
| RU2012150283A (ru) | Усовершенствованный способ культивирования клеток | |
| JP2017520242A5 (enExample) | ||
| RU2008152449A (ru) | Получение гликопротеинов | |
| WO2008154014A3 (en) | A method for culturing mammalian cells to improve recombinant protein production | |
| EP4148121A3 (en) | Cell-controlled perfusion in continuous culture | |
| MX2012003654A (es) | Metodos de produccion de glucoproteinas en cultivos de celulas de mamiferos usando glucocorticoides. | |
| IN2013MU02285A (enExample) | ||
| TR201900213T4 (tr) | Bir sürekli hücre kültüründe bir polipeptit veya ilişkin virüsün üretim metodu. | |
| MX2014007907A (es) | Encristalado multiple con difusion variable por cristales liquidos y metodo de fabricacion del mismo. | |
| TW200516148A (en) | Method for recloning production cells | |
| SG10201810830UA (en) | Cell culture compositions and methods for polypeptide production | |
| EA201590550A1 (ru) | Композиции и способы получения гликопротеинов | |
| FI3237432T3 (fi) | Proteiinin valmistus | |
| JP2016513478A5 (enExample) | ||
| EA201492185A1 (ru) | Способ получения рекомбинантной идуронат-2-сульфатазы | |
| BR112016028538A2 (pt) | processo de produção de proteína glicosilada, processos de produção de proteína de fusão e anticorpo monoclonal e fragmento do mesmo, processo de produção de proteína de fusão tnfr-fc, proteína de fusão ou anticorpo monoclonal e fragmento do mesmo | |
| BR112013025203A2 (pt) | "cepas variantes de fundos filamentosos tendo modificação genética para maior produção de proteína seb1 e crescimento alterado e seu método de 5 produção". | |
| AR097805A1 (es) | Células de cambio de temperatura para la expresión de alto rendimiento de polipéptidos en levadura y otros transformados |