BR112016010340A2 - novos métodos para se produzir tert ativa - Google Patents
novos métodos para se produzir tert ativaInfo
- Publication number
- BR112016010340A2 BR112016010340A2 BR112016010340A BR112016010340A BR112016010340A2 BR 112016010340 A2 BR112016010340 A2 BR 112016010340A2 BR 112016010340 A BR112016010340 A BR 112016010340A BR 112016010340 A BR112016010340 A BR 112016010340A BR 112016010340 A2 BR112016010340 A2 BR 112016010340A2
- Authority
- BR
- Brazil
- Prior art keywords
- tert
- new methods
- producing active
- active tert
- active
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
- C12N9/1241—Nucleotidyltransferases (2.7.7)
- C12N9/1276—RNA-directed DNA polymerase (2.7.7.49), i.e. reverse transcriptase or telomerase
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
- A61K38/16—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- A61K38/43—Enzymes; Proenzymes; Derivatives thereof
- A61K38/45—Transferases (2)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
- A61K39/0005—Vertebrate antigens
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P35/00—Antineoplastic agents
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
- C07K14/24—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Enterobacteriaceae (F), e.g. Citrobacter, Serratia, Proteus, Providencia, Morganella, Yersinia
- C07K14/245—Escherichia (G)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
- C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/48—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving transferase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
- C12Y207/07—Nucleotidyltransferases (2.7.7)
- C12Y207/07049—RNA-directed DNA polymerase (2.7.7.49), i.e. telomerase or reverse-transcriptase
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/20—Fusion polypeptide containing a tag with affinity for a non-protein ligand
- C07K2319/24—Fusion polypeptide containing a tag with affinity for a non-protein ligand containing a MBP (maltose binding protein)-tag
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/50—Fusion polypeptide containing protease site
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Medicinal Chemistry (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Biomedical Technology (AREA)
- Immunology (AREA)
- Pharmacology & Pharmacy (AREA)
- Veterinary Medicine (AREA)
- Animal Behavior & Ethology (AREA)
- Public Health (AREA)
- Gastroenterology & Hepatology (AREA)
- Biophysics (AREA)
- Epidemiology (AREA)
- Mycology (AREA)
- Physics & Mathematics (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Plant Pathology (AREA)
- Analytical Chemistry (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Enzymes And Modification Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Peptides Or Proteins (AREA)
- Medicinal Preparation (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
Abstract
a presente invenção se refere a um método para se produzir, reprodutivelmente, grandes quantidades de transcriptase reversa de telomerase (tert) solúvel e ativa ao expressar esta enzima em células de levedura. um rendimento elevado de tert ativa recombinante foi obtido através do controle de parâmetros específicos do processo de purificação. a proteína tert assim obtida mostra atividade com tr transcrita in vitro, abrindo o caminho para o rastreamento com elevado rendimento químico de conjuntos de inibidores de telomerase e aplicações terapêuticas.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
EP20130306526 EP2871235A1 (en) | 2013-11-07 | 2013-11-07 | New methods to produce active hTERT |
PCT/EP2014/074098 WO2015067781A1 (en) | 2013-11-07 | 2014-11-07 | New methods to produce active tert |
Publications (1)
Publication Number | Publication Date |
---|---|
BR112016010340A2 true BR112016010340A2 (pt) | 2018-03-27 |
Family
ID=49679457
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
BR112016010340A BR112016010340A2 (pt) | 2013-11-07 | 2014-11-07 | novos métodos para se produzir tert ativa |
Country Status (10)
Country | Link |
---|---|
US (1) | US20160251636A1 (pt) |
EP (2) | EP2871235A1 (pt) |
JP (1) | JP2016537026A (pt) |
KR (1) | KR20160086865A (pt) |
CN (1) | CN105874063A (pt) |
AU (1) | AU2014345501A1 (pt) |
BR (1) | BR112016010340A2 (pt) |
CA (1) | CA2932212A1 (pt) |
IL (1) | IL245524A0 (pt) |
WO (1) | WO2015067781A1 (pt) |
Families Citing this family (11)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN108289909A (zh) * | 2015-10-19 | 2018-07-17 | 巴尔的摩马里兰大学 | 用于产生工程改造的人原代血液树突细胞系的方法 |
JP7114481B2 (ja) * | 2016-04-07 | 2022-08-08 | ジェムバックス アンド カエル カンパニー,リミティド | テロメラーゼ活性の増加及びテロメアの延長の効能を有するペプチド、及びこれを含む組成物 |
EP3518955A4 (en) * | 2016-09-30 | 2020-10-07 | The Wistar Institute Of Anatomy And Biology | TERT IMMUNOGENIC COMPOSITIONS AND METHODS OF TREATMENT USING THEM |
US20220065857A1 (en) * | 2018-12-12 | 2022-03-03 | The Johns Hopkins University | Hiv serosignatures for cross-sectional incidence estimation |
CN110205337A (zh) * | 2019-06-24 | 2019-09-06 | 王跃驹 | 植物作为宿主表达人源端粒酶的应用 |
CN112921002B (zh) * | 2021-01-15 | 2023-03-31 | 中山大学 | 一种以人内源性tert蛋白为靶点筛选端粒酶调控剂的细胞模型及其制备方法和应用 |
KR102681811B1 (ko) * | 2021-02-18 | 2024-07-05 | 건국대학교 글로컬산학협력단 | Her2에 결합하는 단일 가닥 가변 영역 항체 절편 플랫폼 |
CN113583141B (zh) * | 2021-08-04 | 2023-03-03 | 江西农业大学 | 猪流行性腹泻病毒Nsp9蛋白、含该Nsp9蛋白的融合蛋白及其制备方法和应用 |
CN114107252B (zh) * | 2021-12-02 | 2023-12-29 | 翌圣生物科技(上海)股份有限公司 | CL7蛋白质、高活性重组TET酶CL7-NgTET1、其原核表达载体及应用 |
CN116286562B (zh) * | 2021-12-10 | 2024-09-10 | 虹摹生物科技(上海)有限公司 | 一种基因工程菌及其制备方法和应用 |
CN117362452B (zh) * | 2023-12-06 | 2024-03-08 | 北京质肽生物医药科技有限公司 | 一种表达包含全能核酸酶的融合蛋白的工程菌或其衍生产物在核酸降解中的应用 |
Family Cites Families (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
SG94355A1 (en) * | 1996-10-01 | 2003-02-18 | Geron Corp | Human telomerase catalytic subunit |
US7585622B1 (en) * | 1996-10-01 | 2009-09-08 | Geron Corporation | Increasing the proliferative capacity of cells using telomerase reverse transcriptase |
AU1606999A (en) * | 1997-11-26 | 1999-06-15 | Albert Einstein College Of Medicine Of Yeshiva University | Mouse telomerase reverse transcriptase |
KR20070009269A (ko) * | 2005-07-15 | 2007-01-18 | 한국생명공학연구원 | 재조합단백질 생산용 단백질융합인자 라이브러리 및이로부터 획득된 단백질융합인자 |
US8206949B2 (en) * | 2005-11-15 | 2012-06-26 | Glycofi, Inc. | Production of glycoproteins with reduced O-glycosylation |
EP1994942A1 (en) * | 2007-05-25 | 2008-11-26 | INSERM (Institut National de la Santé et de la Recherche Médicale) | Pharmaceutical compositions comprising telomerase, and uses thereof |
EP2573122A3 (en) * | 2008-12-04 | 2013-07-10 | Korea Research Institute of Bioscience and Biotechnology | Screening of abundantly secreted proteins and their use as fusion partners for the production of recombinant proteins |
-
2013
- 2013-11-07 EP EP20130306526 patent/EP2871235A1/en not_active Withdrawn
-
2014
- 2014-11-07 AU AU2014345501A patent/AU2014345501A1/en not_active Abandoned
- 2014-11-07 WO PCT/EP2014/074098 patent/WO2015067781A1/en active Application Filing
- 2014-11-07 US US15/034,008 patent/US20160251636A1/en not_active Abandoned
- 2014-11-07 BR BR112016010340A patent/BR112016010340A2/pt not_active Application Discontinuation
- 2014-11-07 KR KR1020167015129A patent/KR20160086865A/ko not_active Application Discontinuation
- 2014-11-07 CA CA2932212A patent/CA2932212A1/en not_active Abandoned
- 2014-11-07 JP JP2016552689A patent/JP2016537026A/ja active Pending
- 2014-11-07 EP EP14796747.5A patent/EP3066198A1/en not_active Withdrawn
- 2014-11-07 CN CN201480072260.8A patent/CN105874063A/zh active Pending
-
2016
- 2016-05-08 IL IL245524A patent/IL245524A0/en unknown
Also Published As
Publication number | Publication date |
---|---|
CN105874063A (zh) | 2016-08-17 |
WO2015067781A1 (en) | 2015-05-14 |
IL245524A0 (en) | 2016-06-30 |
EP2871235A1 (en) | 2015-05-13 |
EP3066198A1 (en) | 2016-09-14 |
US20160251636A1 (en) | 2016-09-01 |
CA2932212A1 (en) | 2015-05-14 |
JP2016537026A (ja) | 2016-12-01 |
AU2014345501A1 (en) | 2016-05-26 |
KR20160086865A (ko) | 2016-07-20 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
B11A | Dismissal acc. art.33 of ipl - examination not requested within 36 months of filing | ||
B11Y | Definitive dismissal - extension of time limit for request of examination expired [chapter 11.1.1 patent gazette] |