BR112016010340A2 - novos métodos para se produzir tert ativa - Google Patents

novos métodos para se produzir tert ativa

Info

Publication number
BR112016010340A2
BR112016010340A2 BR112016010340A BR112016010340A BR112016010340A2 BR 112016010340 A2 BR112016010340 A2 BR 112016010340A2 BR 112016010340 A BR112016010340 A BR 112016010340A BR 112016010340 A BR112016010340 A BR 112016010340A BR 112016010340 A2 BR112016010340 A2 BR 112016010340A2
Authority
BR
Brazil
Prior art keywords
tert
new methods
producing active
active tert
active
Prior art date
Application number
BR112016010340A
Other languages
English (en)
Inventor
Segal-Bendirdjian Evelyne
Kellermann Guillaume
Lahuna Olivier
Bombard Sophie
Original Assignee
1 Centre National De La Recherche Scientifique Cnrs 2 Institut National De La Sante Et De La Recherc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 1 Centre National De La Recherche Scientifique Cnrs 2 Institut National De La Sante Et De La Recherc filed Critical 1 Centre National De La Recherche Scientifique Cnrs 2 Institut National De La Sante Et De La Recherc
Publication of BR112016010340A2 publication Critical patent/BR112016010340A2/pt

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1276—RNA-directed DNA polymerase (2.7.7.49), i.e. reverse transcriptase or telomerase
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • A61K38/16—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • A61K38/43—Enzymes; Proenzymes; Derivatives thereof
    • A61K38/45—Transferases (2)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K39/0005—Vertebrate antigens
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P35/00—Antineoplastic agents
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/24—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Enterobacteriaceae (F), e.g. Citrobacter, Serratia, Proteus, Providencia, Morganella, Yersinia
    • C07K14/245—Escherichia (G)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/48—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving transferase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/07—Nucleotidyltransferases (2.7.7)
    • C12Y207/07049—RNA-directed DNA polymerase (2.7.7.49), i.e. telomerase or reverse-transcriptase
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/20—Fusion polypeptide containing a tag with affinity for a non-protein ligand
    • C07K2319/24—Fusion polypeptide containing a tag with affinity for a non-protein ligand containing a MBP (maltose binding protein)-tag
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/50—Fusion polypeptide containing protease site

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biomedical Technology (AREA)
  • Immunology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Veterinary Medicine (AREA)
  • Epidemiology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Mycology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Plant Pathology (AREA)
  • Analytical Chemistry (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Medicinal Preparation (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Peptides Or Proteins (AREA)

Abstract

a presente invenção se refere a um método para se produzir, reprodutivelmente, grandes quantidades de transcriptase reversa de telomerase (tert) solúvel e ativa ao expressar esta enzima em células de levedura. um rendimento elevado de tert ativa recombinante foi obtido através do controle de parâmetros específicos do processo de purificação. a proteína tert assim obtida mostra atividade com tr transcrita in vitro, abrindo o caminho para o rastreamento com elevado rendimento químico de conjuntos de inibidores de telomerase e aplicações terapêuticas.
BR112016010340A 2013-11-07 2014-11-07 novos métodos para se produzir tert ativa BR112016010340A2 (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP20130306526 EP2871235A1 (en) 2013-11-07 2013-11-07 New methods to produce active hTERT
PCT/EP2014/074098 WO2015067781A1 (en) 2013-11-07 2014-11-07 New methods to produce active tert

Publications (1)

Publication Number Publication Date
BR112016010340A2 true BR112016010340A2 (pt) 2018-03-27

Family

ID=49679457

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112016010340A BR112016010340A2 (pt) 2013-11-07 2014-11-07 novos métodos para se produzir tert ativa

Country Status (10)

Country Link
US (1) US20160251636A1 (pt)
EP (2) EP2871235A1 (pt)
JP (1) JP2016537026A (pt)
KR (1) KR20160086865A (pt)
CN (1) CN105874063A (pt)
AU (1) AU2014345501A1 (pt)
BR (1) BR112016010340A2 (pt)
CA (1) CA2932212A1 (pt)
IL (1) IL245524A0 (pt)
WO (1) WO2015067781A1 (pt)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2018531022A (ja) * 2015-10-19 2018-10-25 ユニバーシティ オブ メリーランド ボルチモア 改変ヒト初代血液樹状細胞株を生成するための方法
WO2017176087A1 (ko) * 2016-04-07 2017-10-12 주식회사 젬백스앤카엘 텔로머라제 활성 증가 및 텔로미어 연장 효능을 가지는 펩티드 및 이를 포함하는 조성물
CN110573173A (zh) * 2016-09-30 2019-12-13 宾夕法尼亚大学理事会 Tert免疫原性组合物及使用其的治疗方法
US20220065857A1 (en) * 2018-12-12 2022-03-03 The Johns Hopkins University Hiv serosignatures for cross-sectional incidence estimation
CN110205337A (zh) * 2019-06-24 2019-09-06 王跃驹 植物作为宿主表达人源端粒酶的应用
CN112921002B (zh) * 2021-01-15 2023-03-31 中山大学 一种以人内源性tert蛋白为靶点筛选端粒酶调控剂的细胞模型及其制备方法和应用
CN113583141B (zh) * 2021-08-04 2023-03-03 江西农业大学 猪流行性腹泻病毒Nsp9蛋白、含该Nsp9蛋白的融合蛋白及其制备方法和应用
CN114107252B (zh) * 2021-12-02 2023-12-29 翌圣生物科技(上海)股份有限公司 CL7蛋白质、高活性重组TET酶CL7-NgTET1、其原核表达载体及应用
CN116286562A (zh) * 2021-12-10 2023-06-23 虹摹生物科技(上海)有限公司 一种基因工程菌及其制备方法和应用
CN117362452B (zh) * 2023-12-06 2024-03-08 北京质肽生物医药科技有限公司 一种表达包含全能核酸酶的融合蛋白的工程菌或其衍生产物在核酸降解中的应用

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2001523947A (ja) 1996-10-01 2001-11-27 ジェロン コーポレイション テロメラーゼ逆転写酵素
US7585622B1 (en) * 1996-10-01 2009-09-08 Geron Corporation Increasing the proliferative capacity of cells using telomerase reverse transcriptase
AU1606999A (en) * 1997-11-26 1999-06-15 Albert Einstein College Of Medicine Of Yeshiva University Mouse telomerase reverse transcriptase
KR20070009269A (ko) * 2005-07-15 2007-01-18 한국생명공학연구원 재조합단백질 생산용 단백질융합인자 라이브러리 및이로부터 획득된 단백질융합인자
CN101365783B (zh) * 2005-11-15 2013-07-17 格利科菲公司 具有降低的o-糖基化的糖蛋白的产生
EP1994942A1 (en) * 2007-05-25 2008-11-26 INSERM (Institut National de la Santé et de la Recherche Médicale) Pharmaceutical compositions comprising telomerase, and uses thereof
KR101387693B1 (ko) * 2008-12-04 2014-04-21 한국생명공학연구원 고분비성 단백질의 스크리닝 및 재조합 단백질의 생산을 위한 융합 파트너로서 그의 용도

Also Published As

Publication number Publication date
EP3066198A1 (en) 2016-09-14
WO2015067781A1 (en) 2015-05-14
CN105874063A (zh) 2016-08-17
IL245524A0 (en) 2016-06-30
EP2871235A1 (en) 2015-05-13
AU2014345501A1 (en) 2016-05-26
US20160251636A1 (en) 2016-09-01
JP2016537026A (ja) 2016-12-01
KR20160086865A (ko) 2016-07-20
CA2932212A1 (en) 2015-05-14

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B11A Dismissal acc. art.33 of ipl - examination not requested within 36 months of filing
B11Y Definitive dismissal - extension of time limit for request of examination expired [chapter 11.1.1 patent gazette]