CN102858952B - 改进的细胞培养基 - Google Patents

改进的细胞培养基 Download PDF

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Publication number
CN102858952B
CN102858952B CN201180021024.XA CN201180021024A CN102858952B CN 102858952 B CN102858952 B CN 102858952B CN 201180021024 A CN201180021024 A CN 201180021024A CN 102858952 B CN102858952 B CN 102858952B
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cell culture
medium
cells
culture medium
concentration
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Chinese (zh)
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CN102858952A (zh
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C·莱斯特
P·迈斯纳
J·施密特
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Novartis AG
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Novartis AG
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0018—Culture media for cell or tissue culture
    • C12N5/0031—Serum-free culture media
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0018—Culture media for cell or tissue culture
    • C12N5/005—Protein-free medium
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0018—Culture media for cell or tissue culture
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/05—Inorganic components
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/05—Inorganic components
    • C12N2500/10—Metals; Metal chelators
    • C12N2500/12—Light metals, i.e. alkali, alkaline earth, Be, Al, Mg

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • Cell Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
CN201180021024.XA 2010-04-26 2011-04-25 改进的细胞培养基 Active CN102858952B (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US32783610P 2010-04-26 2010-04-26
US61/327,836 2010-04-26
PCT/EP2011/056508 WO2011134920A1 (en) 2010-04-26 2011-04-25 Improved cell culture medium

Publications (2)

Publication Number Publication Date
CN102858952A CN102858952A (zh) 2013-01-02
CN102858952B true CN102858952B (zh) 2015-10-14

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CN201180021024.XA Active CN102858952B (zh) 2010-04-26 2011-04-25 改进的细胞培养基

Country Status (17)

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US (2) USRE48864E1 (enExample)
EP (1) EP2563904B1 (enExample)
JP (3) JP6347949B2 (enExample)
KR (1) KR101828623B1 (enExample)
CN (1) CN102858952B (enExample)
AU (1) AU2011246503B2 (enExample)
BR (1) BR112012027282B1 (enExample)
CA (1) CA2797356C (enExample)
DK (1) DK2563904T3 (enExample)
ES (1) ES2533074T3 (enExample)
IL (1) IL222452B (enExample)
MX (1) MX2012012526A (enExample)
PL (1) PL2563904T3 (enExample)
PT (1) PT2563904E (enExample)
RU (1) RU2644651C2 (enExample)
SG (1) SG185038A1 (enExample)
WO (1) WO2011134920A1 (enExample)

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US9249182B2 (en) 2012-05-24 2016-02-02 Abbvie, Inc. Purification of antibodies using hydrophobic interaction chromatography
JP6271536B2 (ja) 2012-07-09 2018-01-31 コヒラス・バイオサイエンシズ・インコーポレイテッド 肉眼不可視粒子の著しい低減を示すエタネルセプト製剤
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KR20150043523A (ko) 2012-09-02 2015-04-22 애브비 인코포레이티드 단백질 불균일성의 제어 방법
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US9017687B1 (en) 2013-10-18 2015-04-28 Abbvie, Inc. Low acidic species compositions and methods for producing and using the same using displacement chromatography
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EP3052640A2 (en) 2013-10-04 2016-08-10 AbbVie Inc. Use of metal ions for modulation of protein glycosylation profiles of recombinant proteins
DK3351620T3 (da) * 2013-10-11 2020-10-26 Regeneron Pharma Metabolsk optimeret cellekultur
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US9181337B2 (en) 2013-10-18 2015-11-10 Abbvie, Inc. Modulated lysine variant species compositions and methods for producing and using the same
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EP4332231A3 (en) 2014-04-29 2024-05-29 Novartis AG Novel vertebrate cells and methods for recombinantly expressing a polypeptide of interest
ES2733298T3 (es) 2014-07-18 2019-11-28 Sandoz Ag Cuantificación de TNFR2:Fc plegado erróneamente
CN108495926A (zh) * 2015-12-17 2018-09-04 生命技术公司 用于细胞培养的颗粒及其制备方法
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BR112021021349A2 (pt) 2019-04-26 2022-02-01 Allogene Therapeutics Inc Métodos de fabricação de células car t alogênicas
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KR20230002642A (ko) * 2020-04-15 2023-01-05 제넨테크, 인크. 접종 전 배지를 처리하는 방법
US20230313259A1 (en) * 2020-08-14 2023-10-05 Bristol-Myers Squibb Company Manufacturing process for protein
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CN114606200B (zh) * 2022-02-10 2025-07-18 上海迈邦生物科技有限公司 Sp2/0细胞培养基及生产重组犬细小病毒单克隆抗体的方法
WO2026009803A1 (ja) * 2024-07-05 2026-01-08 国立大学法人九州大学 ヘパリン様物質を製造する方法

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Also Published As

Publication number Publication date
CA2797356C (en) 2020-12-29
PT2563904E (pt) 2015-05-12
ES2533074T3 (es) 2015-04-07
CN102858952A (zh) 2013-01-02
KR101828623B1 (ko) 2018-02-12
AU2011246503B2 (en) 2013-09-26
US9428727B2 (en) 2016-08-30
US20130122543A1 (en) 2013-05-16
IL222452B (en) 2019-03-31
SG185038A1 (en) 2012-11-29
EP2563904B1 (en) 2015-01-21
BR112012027282A2 (pt) 2015-09-22
PL2563904T3 (pl) 2015-06-30
DK2563904T3 (en) 2015-04-07
RU2012150346A (ru) 2014-06-10
AU2011246503A1 (en) 2012-11-22
CA2797356A1 (en) 2011-11-03
RU2644651C2 (ru) 2018-02-13
USRE48864E1 (en) 2021-12-28
EP2563904A1 (en) 2013-03-06
JP2017012180A (ja) 2017-01-19
RU2018101201A3 (enExample) 2021-05-27
JP2013524825A (ja) 2013-06-20
JP7034991B2 (ja) 2022-03-14
RU2018101201A (ru) 2019-02-21
BR112012027282B1 (pt) 2020-09-29
JP6603628B2 (ja) 2019-11-06
MX2012012526A (es) 2012-11-23
KR20130058699A (ko) 2013-06-04
JP2019216728A (ja) 2019-12-26
JP6347949B2 (ja) 2018-06-27
WO2011134920A1 (en) 2011-11-03
IL222452A0 (en) 2012-12-31

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