JP6234003B2 - Vegf産生促進剤 - Google Patents
Vegf産生促進剤 Download PDFInfo
- Publication number
- JP6234003B2 JP6234003B2 JP2012005150A JP2012005150A JP6234003B2 JP 6234003 B2 JP6234003 B2 JP 6234003B2 JP 2012005150 A JP2012005150 A JP 2012005150A JP 2012005150 A JP2012005150 A JP 2012005150A JP 6234003 B2 JP6234003 B2 JP 6234003B2
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- JP
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- Prior art keywords
- vegf
- hair
- aloe
- vegf production
- extract
- Prior art date
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Description
項1.アロエエキスを有効成分とするVEGF産生促進剤。
項2.前記アロエエキスが、アロエ植物体の水、エタノール及び1,3−ブチレングリコールよりなる群から選ばれる少なくとも1種の抽出溶媒で抽出されたものである、前記項1に記載のVEGF産生促進剤。
項3.前記アロエエキスが、キダチアロエ及び/又はアロエベラから抽出されたものである、前記項1又は2のいずれかに記載のVEGF産生促進剤。
項4.アロエエキスを有効成分とするVEGF遺伝子発現量増加剤。
アロエエキスを調製するためのアロエは、特に限定されないが、医薬部外品に使用が認められているという理由から、キダチアロエ(学名:Aloe arborescens)、アロエベラ(学名:Aloe vera)が好ましい。
本発明のVEGF(血管内皮増殖因子、vascular endothelial growth factor)産生促進剤は、前記アロエエキスを有効成分として含有するものである。
アロエエキスのVEGF産生促進及びVEGF遺伝子発現量増加は、ヒト頭髪毛乳頭細胞(HFDPC)を使用して、評価することができる。
以下の手順で、毛乳頭細胞によって培養液中に分泌されたVEGFをELISAにより測定する。
以下の手順で、毛乳頭細胞からtotal RNAを抽出し、VEGFの遺伝子発現解析を行う。
アロエ(キダチアロエ、アロエベラ)(乾燥物の植物体として10g)を細かく刻み、その粉砕物に、10倍量の水、エタノール又は1,3−ブチレングリコール(100mL)を加えて、2日間静置し、エキス(抽出液)を得た。
VEGF産生量及びVEGF遺伝子発現量を、ヒト頭髪毛乳頭細胞(HFDPC)(東洋紡績株式会社製)を使用して、評価した。
以下の手順で、毛乳頭細胞によって培養液中に分泌されたVEGFをELISAにより測定した。
以下の手順で、毛乳頭細胞からtotal RNAを抽出し、VEGFの遺伝子発現解析を行った。
更に、以下の手順で、5-α-リダクターゼの活性測定を行った。
毛乳頭細胞が産生したVEGF量、VEGFの遺伝子発現量、FGF-7の遺伝子発現量及び5-α-リダクターゼ活性抑制程度について、有意差検定を行い、下記の基準に従って評価した。
○:有意に効果有り(p<0.05)
△:効果有り(cut off値以上、ポジティブコントロールの10%以上の効果有り)
−:効果なし
N.D.:未測定又は評価不能
試験結果を下記表3に示した。
Claims (3)
- アロエエキスを有効成分とする、毛乳頭細胞のVEGF産生促進剤。
- 前記アロエエキスが、アロエ植物体の水、エタノール及び1,3−ブチレングリコールよりなる群から選ばれる少なくとも1種による抽出物である、請求項1に記載のVEGF産生促進剤。
- 前記アロエ植物体が、キダチアロエ及び/又はアロエベラである、請求項1又は2のいずれかに記載のVEGF産生促進剤。
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