JP6201054B2 - モノアセチルジアシルグリセロール化合物を有効成分として含有する血液癌または癌転移抑制用組成物 - Google Patents
モノアセチルジアシルグリセロール化合物を有効成分として含有する血液癌または癌転移抑制用組成物 Download PDFInfo
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- JP6201054B2 JP6201054B2 JP2016536021A JP2016536021A JP6201054B2 JP 6201054 B2 JP6201054 B2 JP 6201054B2 JP 2016536021 A JP2016536021 A JP 2016536021A JP 2016536021 A JP2016536021 A JP 2016536021A JP 6201054 B2 JP6201054 B2 JP 6201054B2
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Description
人間細胞株U937、A549、Jurkat、HEK293と家ネズミ細胞株RBL-2H3(American Type Culture Collection、ATCC、Rockville、MD)の培養は37℃, 5% CO2の湿化された条件下で遂行した。U937、Jurkat、K562と A549細胞株は10%ウシ胎仔血清(Fetal Calf Serum、FCS、HyClone、Logan、UT)、2 mM L-グルタメート、100 μg/mlペニシリン、100 μg/ml ストレプトマイシン(Life Technologies)を含むRPMI1640(Life Technologies、Karlsruhe、Germany)培地、HEK293細胞株はDMEM培地、RBL-2H3 細胞株はMEM培地で培養した。
EC-18とIL-4とIFN-γを処理した細胞に冷たいSDS-溶解バッファー[(50 mM HEPES、150 mM NaCl、0.2 mM EDTA、0.5% NP-40、0.1% SDS、1mM Na3VO4、10mM NaF、and complete Protein Inhibitor Cocktail(Roche)]を使って30分間氷に溶解した。溶解された細胞溶液を高速回転機で30分間13,000 rpmで回転して不溶性部分を沈殿させて水溶性溶液を分離した。分離した細胞水様液を定量後10〜12% のぐらい濃度のSDS-PAGEを利用して電気泳動した。Gel上で分離された細胞蛋白質をPVDFメンブレン(Millipore、Billerica、MA、 USA)で100Vから2時間転写させた。
細胞内で燐酸化されたSTAT-1、STAT-6を確認するためpoly rabbit anti-(STAT1, STAT6), -phosphor(STAT1, STAT6)(Cell signaling Technology, USA)(1:1000)を1次抗体に利用して室温で60分間反応させた。2次抗体ではhorseradish peroxidase peroxidase-conjugated goat anti-rabbit IgG(Santa Cruz Biotechnology, USA)(1:3000)で室温で60分間反応させた。細胞蛋白質の同一量の測定はpoly rabbit anti-(STAT1, STAT6)で確認した。免疫反応が終った メンブレンはECL試薬(Millipore, Billerica, MA, USA)で反応させて、X-rayフイルムに露出させてSTATの燐酸化程度をフイルム上に現れたバンドで確認した。
STAT-6が結合するSTAT-6Elementを含めているp4xSTAT6-Luc2Pベクターを利用してHEK293細胞はA549細胞に導入させた後EC-18を前処理してIL-4を処理してSTAT-6の活性抑制を分析した。また、STAT-1が結合するInterferon Stimulated Response Element(ISRE)を含めているpGL4.45[luc2P/ISRE/Hygro]ベクター(Promega)を利用してSTAT-1の活性を分析した。
実験例 3-1: 逆転写ポリメラーゼ連鎖反応(RT-PCR)
RBL-2H3細胞にEC-18を前処理した後、IgEに対したantigenで細胞活性を誘導させてその結果で発現されるサイトカインIL-4のmRNA水準の変化をRT-PCR(Reverse Transcriptase Polymerase Chain Reaction)を遂行した。
細胞は0.01、0.1、1.0及び10μg/mlのEC-18をそれぞれ1時間前処理してAntigenを一緒に処理した後、3時間培養した。 続いて、細胞を溶解させた後にRNAを分離した。 RNAに対してpoly A+プライマーと共に逆転写酵素(reverse transcriptase)を使用してcDNAを製造した。 IL-4についてデザインされたプライマーをPCR増幅に使用した(表1)。GAPDHを内部標準として使用した。
EC-18を0.1 pg/ml乃至10μg/mlの濃度になるように処理して1時間反応させて準備して抗原を処理して37℃で18時間培養した後、細胞を除去して上層液を修得した。 RBL-2H3細胞の細胞培養液に存在する家ネズミIL-4の定量は商業的に購入可能なモノクローナル抗体(mAb)(BD Biosciences)を使用して製造者が指示したプロトコルに従って酵素結合免疫吸収分析(enzyme-linked immunosorbent assay、ELISA)を遂行した。
人体由来の骨髄癌細胞株であるRPMI 8226細胞が移植された雄のヌード・マウスに試験物質であるEC-18を経口投与した後腫瘍の成長抑制効果を評価した。 群構成は陰性対照群、500 mg/kgの容量の試験物質投与群、500 mg/kgの容量の試験物質及び80 mg/kgの容量の陽性対照物質ゲムシタビン(Gemcitabine)併用の投与群、500 mg/kgの容量の試験物質及び120 mg/kgの容量の陽性対照物質併用の投与群、80及び120 mg/kgの容量の陽性対照群の総6この群で構成し、各群当10匹ずつ実験した。 陰性対照群は賦形剤であるolive oilを、試験物質投与群は試験物質(EC-18)を1回/日、4週間、総28回員内に強制投与し、陽性対照群は陽性対照物質であるゲムシタビン(Gemcitabine)を2回/週、4週間、総8回腹腔に強制投与した。 観察期間の間、毎日1回一般症状を観察し、動物の体重及び腫瘍の体積(Tumor volume、mm3)は2回/週測定した。 観察期間終了後腫瘍を摘出して腫瘍の重量(Tumor weight、g)を測定し、それぞれ図6及び7に示した。図6及び7に示したとおり、500 mg/kgの容量の試験物質投与群は腫瘍の体積測定結果、陰性対照群と比較して統計学的に有意に抑制され、腫瘍の重量も陰性対照群と比較して統計学的に有意に小さく測定され、腫瘍の成長を抑制した。 また、血液癌モデルハツカネズミでEC-18と陽性対照群であるgemcitabineに投与した後、腫瘍の大きさ変化を示す写真を図8に図示した。 図8に図示されたとおり、EC-18を投与した場合にも、陽性対照群と類似に腫瘍の大きさが減少されることを確認できる。
Claims (12)
- 前記R1およびR2はそれぞれパルミトイル(palmitoyl)、オレオイル(oleoyl)、リノレオイル(linoleoyl)、リノレノイル(linolenoyl)、ステアロイル(stearoyl)、ミリストイル(myristoyl)、アラキドノイル(arachidonoyl)で構成される群から選択されることを特徴とする請求項1に記載の組成物。
- 前記R1およびR2の組み合わせ(R1/R2)はオレオイル/パルミトイル、パルミトイル/オレオイル、パルミトイル/リノレオイル、パルミトイル/リノレノイル、パルミトイル/アラキドノイル、パルミトイル/ステアロイル、パルミトイル/パルミトイル、オレオイル/ステアロイル、リノレオイル/パルミトイル、リノレオイル/ステアロイル、ステアロイル/リノレオイル、ステアロイル/オレオイル、ミリストイル/リノレオイル、ミリストイル/オレオイルで構成される群から選択されることを特徴とする請求項1または2に記載の組成物。
- 前記モノアセチルジアシルグリセロール化合物は鹿茸から分離したことを特徴とする請求項1〜4のいずれか1項に記載の組成物。
- 前記モノアセチルジアシルグリセロール化合物は、IL−4の分泌を抑制することを特徴とする、請求項1〜5のいずれか1項に記載の組成物。
- 前記モノアセチルジアシルグリセロール化合物は、STAT−6の活性を抑制することを特徴とする、請求項1〜6のいずれか1項に記載の組成物。
- 前記R1およびR2はそれぞれパルミトイル(palmitoyl)、オレオイル(oleoyl)、リノレオイル(linoleoyl)、リノレノイル(linolenoyl)、ステアロイル(stearoyl)、ミリストイル(myristoyl)、アラキドノイル(arachidonoyl)で構成される群から選択されることを特徴とする、請求項9に記載の組成物。
- 前記R1およびR2の組み合わせ(R1/R2)はオレオイル/パルミトイル、パルミトイル/オレオイル、パルミトイル/リノレオイル、パルミトイル/リノレノイル、パルミトイル/アラキドノイル、パルミトイル/ステアロイル、パルミトイル/パルミトイル、オレオイル/ステアロイル、リノレオイル/パルミトイル、リノレオイル/ステアロイル、ステアロイル/リノレオイル、ステアロイル/オレオイル、ミリストイル/リノレオイル、ミリストイル/オレオイルで構成される群から選択されることを特徴とする、請求項9または10に記載の組成物。
- 請求項1乃至8のうちいずれか一項に記載の組成物を非人間個体に投与する段階を含むことを特徴とする、血液癌の予防または治療方法であって、該血液癌は、リンパ腫、急性白血病、慢性白血病、および多発性骨髄腫からなる群より選択される1種である、方法。
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