JP6161541B2 - 細菌中の機能的タンパク質発現を向上させるための方法および物質 - Google Patents

細菌中の機能的タンパク質発現を向上させるための方法および物質 Download PDF

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JP6161541B2
JP6161541B2 JP2013552685A JP2013552685A JP6161541B2 JP 6161541 B2 JP6161541 B2 JP 6161541B2 JP 2013552685 A JP2013552685 A JP 2013552685A JP 2013552685 A JP2013552685 A JP 2013552685A JP 6161541 B2 JP6161541 B2 JP 6161541B2
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antibody
fab
fkpa
host cell
functional
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JP2014505482A (ja
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ラファエル デービッド レビー
ラファエル デービッド レビー
チュン−リョン チャン
チュン−リョン チャン
キランジト カウル アフルワリア
キランジト カウル アフルワリア
トシヒコ タケウチ
トシヒコ タケウチ
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ゾーマ テクノロジー リミテッド
ゾーマ テクノロジー リミテッド
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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/24—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Enterobacteriaceae (F), e.g. Citrobacter, Serratia, Proteus, Providencia, Morganella, Yersinia
    • C07K14/245—Escherichia (G)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/90—Isomerases (5.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y502/00—Cis-trans-isomerases (5.2)
    • C12Y502/01—Cis-trans-Isomerases (5.2.1)
    • C12Y502/01008—Peptidylprolyl isomerase (5.2.1.8), i.e. cyclophilin
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/35—Fusion polypeptide containing a fusion for enhanced stability/folding during expression, e.g. fusions with chaperones or thioredoxin

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Virology (AREA)
  • Immunology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
JP2013552685A 2011-02-03 2012-02-03 細菌中の機能的タンパク質発現を向上させるための方法および物質 Active JP6161541B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201161439232P 2011-02-03 2011-02-03
US61/439,232 2011-02-03
PCT/US2012/023801 WO2012106615A1 (en) 2011-02-03 2012-02-03 Methods and materials for enhancing functional protein expression in bacteria

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JP2014505482A JP2014505482A (ja) 2014-03-06
JP6161541B2 true JP6161541B2 (ja) 2017-07-12

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JP2013552685A Active JP6161541B2 (ja) 2011-02-03 2012-02-03 細菌中の機能的タンパク質発現を向上させるための方法および物質

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US (1) US9732143B2 (zh)
EP (1) EP2670847B1 (zh)
JP (1) JP6161541B2 (zh)
CN (1) CN103492562B (zh)
AU (1) AU2012212047A1 (zh)
CA (1) CA2826142A1 (zh)
DK (1) DK2670847T3 (zh)
WO (1) WO2012106615A1 (zh)

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US9688735B2 (en) * 2010-08-20 2017-06-27 Wyeth Llc Designer osteogenic proteins
US9301971B2 (en) 2013-03-08 2016-04-05 Novartis Ag Peptides and compositions for treatment of joint damage
UY35368A (es) 2013-03-08 2014-10-31 Irm Llc Péptidos y composiciones para el tratamiento de daño articular
US9951114B2 (en) * 2013-06-04 2018-04-24 Virginia Commonwealth University Recombinant cancer therapeutic cytokine
US11072647B2 (en) * 2013-12-19 2021-07-27 Onsejo Nacional DE Investigation Cientiticay Tecnica TGF-receptor II isoform, fusion peptide, methods of treatment and methods in vitro
MX2017000116A (es) 2014-06-30 2017-05-30 Altor Bioscience Corp Moleculas basadas en il-15 y metodos para su uso.
GB201412290D0 (en) 2014-07-10 2014-08-27 Cambridge Entpr Ltd Novel use
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ES2819256T3 (es) 2014-11-05 2021-04-15 Genentech Inc Métodos para producir proteínas bicatenarias en bacterias
AU2015342961B2 (en) 2014-11-05 2021-08-12 Genentech, Inc. Methods of producing two chain proteins in bacteria
ITMI20150558A1 (it) * 2015-04-16 2016-10-16 Univ Politecnica Delle Marche Irisina per la cura e la prevenzione dell'osteoporosi
US11571462B2 (en) * 2015-06-03 2023-02-07 The Medical College Of Wisconsin, Inc. Engineered CCL20 locked dimer polypeptide
WO2016196429A1 (en) * 2015-06-03 2016-12-08 The Medical College Of Wisconsin, Inc. An engineered ccl20 locked dimer polypeptide
WO2017058923A1 (en) * 2015-09-28 2017-04-06 East Carolina University Aluminum based adjuvants for tolerogenic vaccination
EP3393494A1 (en) * 2015-12-22 2018-10-31 Novartis Ag Methods of treating or ameliorating metabolic disorders using growth differentiation factor 15 (gdf-15)
EP3439686A4 (en) * 2016-04-06 2019-10-09 Acceleron Pharma Inc. BMPRII POLYPEPTIDES AND USES THEREOF
RS62011B1 (sr) * 2016-07-15 2021-07-30 Acceleron Pharma Inc Kompozicije koje sadrže polipeptide actriia za upotrebu u lečenju plućne hipertenzije
US11285191B2 (en) * 2016-07-26 2022-03-29 The Board Of Supervisors Of Louisiana State University And Agricultural And Mechanical College Immunostimulatory compositions and uses therefor
IL247369B (en) * 2016-08-18 2018-08-30 B G Negev Tech And Applications Ltd Polypeptides derived from CSF-m and their uses
WO2018187209A1 (en) * 2017-04-03 2018-10-11 Acceleron Pharma Inc. Compositions and methods for treating spinal muscular atrophy
AR114294A1 (es) * 2017-11-30 2020-08-19 Grifols Diagnostic Solutions Inc Inmunoensayos y proteínas de diseño para la monitorización de tratamientos con anticuerpos contra los inhibidores de puntos de control inmunológicos pd-1 y pd-l1
TWI724392B (zh) * 2018-04-06 2021-04-11 美商美國禮來大藥廠 生長分化因子15促效劑化合物及其使用方法
EP3553081A1 (en) * 2018-04-12 2019-10-16 Bayer Aktiengesellschaft Atrial natriuretic peptide engrafted antibodies
MX2020011529A (es) * 2018-05-01 2021-01-29 Ambrx Inc Un metodo para optimizar la expresion de anticuerpos.
TW202027794A (zh) 2018-10-03 2020-08-01 瑞士商諾華公司 血管生成素樣３多肽之持續遞送
CN112638935A (zh) * 2019-01-27 2021-04-09 中国医药大学 一种类风湿性关节炎自体抗体结合的肽及其应用
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US11851490B2 (en) * 2019-08-15 2023-12-26 Northwestern University Methods and compositions for treating, inhibiting, and/or preventing heterotopic ossification
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DK2670847T3 (en) 2017-01-16
JP2014505482A (ja) 2014-03-06
CA2826142A1 (en) 2012-08-09
EP2670847A1 (en) 2013-12-11
CN103492562B (zh) 2017-04-05
CN103492562A (zh) 2014-01-01
US20140154743A1 (en) 2014-06-05
AU2012212047A1 (en) 2013-08-22
WO2012106615A1 (en) 2012-08-09
EP2670847B1 (en) 2016-10-05
US9732143B2 (en) 2017-08-15

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