JP6116490B2 - 分析対象の細胞を含む試料流体の調製に用いる使い捨てカートリッジ - Google Patents
分析対象の細胞を含む試料流体の調製に用いる使い捨てカートリッジ Download PDFInfo
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- JP6116490B2 JP6116490B2 JP2013557222A JP2013557222A JP6116490B2 JP 6116490 B2 JP6116490 B2 JP 6116490B2 JP 2013557222 A JP2013557222 A JP 2013557222A JP 2013557222 A JP2013557222 A JP 2013557222A JP 6116490 B2 JP6116490 B2 JP 6116490B2
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- Apparatus Associated With Microorganisms And Enzymes (AREA)
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- Sampling And Sample Adjustment (AREA)
Description
1つ以上の並列な調製部を含み、各調製部は、
壊れやすい封止材に挟まれて密封され、直列接続された1つ以上のチャンバを含み、各チャンバは、入力流体を受け取り、前記流体に作用する手順を実施して出力流体を発生させ、前記出力流体を放出するように構成されており、
前記1つ以上のチャンバのうちの第1のチャンバが、第1の開口と結合された圧迫可能なチャンバであり、
前記1つ以上のチャンバのうちの最後のチャンバが、第2の開口と結合されており、
前記第1のチャンバの入力流体は、前記試料流体であり、
これら1つ以上の調製部は、第2の開口を介して搬送可能な各出力流体の分析を実施するコンパートメントと結合可能である。
搬送具により試料流体を前記第1のチャンバに投入する前に、前記第1の開口を通る、前記第1のチャンバからの流れを阻止するように構成されている第1の壊れやすい封止材と、
試料流体の投入後に流体がその空間を通って流れるのを防ぐように構成されている第2の封止材と、を含む。
少なくとも1つの分析部を含み、各分析部は、
前記派生物のうちの1つ以上を取得することと、前記派生物を、分析が可能となるように提示することと、を行うように構成された中空部材を含む。
前記チャンバと結合された、断面積の小さいチャネルを備え、この断面積の小さいチャネルは、チャンバ内の前記試料流体の派生物の流れを減速させるように構成されている。
少なくとも2つの接続されたチャンバの系列を含み、前記少なくとも2つのチャンバは第1のチャンバ及び最後のチャンバを含み、前記系列内の各チャンバは、壊れやすい封止材に挟まれて密封されており、入力が前記試料流体の第1の派生物であり、出力が前記試料流体の第2の派生物である手順を実施するように構成されており、
前記系列内の各チャンバは、連続する手順を実施するように構成されており、
第1のチャンバによって取得可能な第1の派生物は、試料流体であり、前記第1のチャンバ以外の全てのチャンバのそれぞれによって取得可能な第1の派生物は、系列内の各先行チャンバのそれぞれの第2の派生物である。
第1の派生物を取得する前にそれぞれのチャンバを封止するように構成された先行封止材と、
それぞれのチャンバから第2の派生物を放出する為に破れるように構成された後続封止材と、を含む。
Claims (18)
- 生体液分析器内で使用するためのカートリッジであって、当該カートリッジは、
試薬材料を含んでいる少なくとも1つのリザーバであって、当該少なくとも1つのリザーバは、弾性材料から作られた圧迫可能部分を含んでおり、前記圧迫可能部分は、外力を受けるように構成される、少なくとも1つのリザーバと、
第1の封止材を含んでいる第1のチャネルであって、前記第1のチャネルは、前記第1の封止材を介して前記少なくとも1つのリザーバ内に前記生体液を受けるために構成される、第1のチャネルと、
第2の封止材を含んでいる第2のチャネルであって、前記第2の封止材は、破壊可能であり、前記少なくとも1つのリザーバの前記圧迫可能部分にかけられる、閾値を超える圧力に応じて、前記少なくとも1つのリザーバから出力された流体を解放するために構成され、前記少なくとも1つのリザーバの前記圧迫可能部分及び前記第2の封止材は、前記第2の封止材の前記閾値を超える圧力より低い圧力での、前記少なくとも1つのリザーバの前記圧迫可能部分の断続的な圧迫及び解放が、前記生体液との前記試薬材料の混合を来たすように構成される、第2のチャネルと、を備える、
カートリッジ。 - 前記試薬材料は、流体の形態の少なくとも1つの高分子ポリマーを含む、請求項1に記載のカートリッジ。
- 前記生体液が、血液である、請求項1に記載のカートリッジ。
- 前記少なくとも1つのリザーバは、前記出力された流体の分析をするために、分析コンパートメントに接続可能である、請求項1に記載のカートリッジ。
- 前記出力された流体は、前記第2のチャネルを介して前記分析コンパートメントに運ばれる、請求項4に記載のカートリッジ。
- 前記第1の封止材は、前記生体液の搬送具を受けて、前記第1のチャネルからの前記試薬又は前記生体液の漏れ無しで前記搬送具を保つように構成される、請求項1に記載のカートリッジ。
- 前記第2の封止材は、再封止可能な封止材である、請求項1に記載のカートリッジ。
- 血液分析器内で使用するための使い捨てカートリッジであって、
血液を含む流体を受けるための入口と、
第1の試薬コンパートメントと、
前記第1の試薬コンパートメント内に前もって収容され、封止された第1の試薬と、
第2の試薬コンパートメントと、
前記第2の試薬コンパートメント内に前もって収容され、封止された第2の試薬と、
前記第1の試薬コンパートメントと前記第2の試薬コンパートメントとを相互に接続するチャネルであって、当該チャネルと、前記第1の試薬コンパートメントと、前記第2の試薬コンパートメントとが、流体流路を画定する、チャネルと、
前記流体流路に含まれている少なくとも1つの封止材であって、前記カートリッジが血液分析器に搭載された後、前記流体流路に与えられた圧力が所定の閾値を超えたことに応じて破れる、封止材と、を備え、
前記カートリッジは、前記第1の試薬コンパートメントに関連づけられた圧迫可能部分の、前記血液分析器による、断続的な圧迫及び解放を介して、前記第1の試薬が前記流体と混合することを可能とするように構成されており、前記カートリッジは、前記血液分析器が前記少なくとも1つの封止材を破り、前記流体及び前記第1の試薬の混合物の少なくとも一部を前記第2の試薬と更に混合させることを可能とするように構成され、前記第1の試薬コンパートメントの前記圧迫可能部分及び前記第2の試薬コンパートメントの圧迫可能部分の断続的な圧迫及び解放が、前記流体、前記第1の試薬、及び前記第2の試薬の流れよる、前記流体、前記第1の試薬、及び前記第2の試薬の混合を引き起こし、当該流れは、前記チャネルを介して前記第1の試薬コンパートメントと前記第2の試薬コンパートメントとの間を行きつ戻りつし、
前記カートリッジは、第2の封止材を含んでいる第2のチャネルであって、前記第2の封止材は、破壊可能であり、前記第2の試薬コンパートメントの前記圧迫可能部分にかけられる、閾値を超える圧力に応じて、前記第2の試薬コンパートメントから出力された前記流体を解放するために構成され、前記第2の試薬コンパートメントの前記圧迫可能部分及び前記第2の封止材は、前記第2の封止材の前記閾値を超える圧力より低い圧力での、前記第1の試薬コンパートメント及び前記第2の試薬コンパートメントの前記圧迫可能部分の断続的な圧迫及び解放が、少なくとも前記流体との前記第2の試薬の混合を来たすように構成される、第2のチャネル、を備える、
使い捨てカートリッジ。 - 前記使い捨てカートリッジは、第3の試薬コンパートメントと、前記第3の試薬コンパートメント内に前もって収容され、封止された第3の試薬と、をさらに含む、請求項8に記載の使い捨てカートリッジ。
- 前記使い捨てカートリッジは、第3の試薬コンパートメントに関連づけられた封止材であって、前記カートリッジが前記血液分析器に搭載された後、前記第3の試薬コンパートメントに与えられた圧力が所定の閾値を超えたことに応じて破れる、封止材と、をさらに含む、請求項8に記載の使い捨てカートリッジ。
- 前記少なくとも1つの封止材は、複数の封止材を含み、前記カートリッジは前記血液分析器に当該複数の封止材のうちの各々を異なるタイミングで破れさせる、請求項8に記載の使い捨てカートリッジ。
- 前記第1の試薬コンパートメント及び前記第2の試薬コンパートメントのうちの少なくとも1つは、出力された流体の分析をするために、分析コンパートメントに接続可能である、請求項8に記載の使い捨てカートリッジ。
- 前記少なくとも1つの封止材は、前記封止材に損傷がないときには、前記第1の試薬又は前記第2の試薬が前記入口を介して流れることを防ぐように構成される封止材を含む、請求項8に記載の使い捨てカートリッジ。
- 前記第1の試薬が前記入口を介して流れるのを防ぐように構成される前記封止材は、再封止可能な封止材である、請求項13に記載の使い捨てカートリッジ。
- 前記少なくとも1つの封止材は、前記封止材に損傷がないときには、前記第1の試薬又は前記第2の試薬が前記チャネルを介して流れることを防ぐように構成される封止材を含む、請求項8に記載の使い捨てカートリッジ。
- 前記第1の試薬コンパートメント及び前記第2の試薬コンパートメントのうちの少なくとも1つは、開口を介して分析コンパートメントに出力された流体を運ぶように構成される、請求項8に記載の使い捨てカートリッジ。
- 前記少なくとも1つの封止材は、前記封止材に損傷がないときには、前記第1の試薬又は前記第2の試薬が前記開口を介して流れることを防ぐように構成される封止材を含む、請求項16に記載の使い捨てカートリッジ。
- 前記第1の試薬及び前記第2の試薬のうちの少なくとも1つは、流体の形態の少なくとも1つの高分子ポリマーを含む、請求項8に記載の使い捨てカートリッジ。
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CN103608110B (zh) | 2017-06-06 |
US20200240879A1 (en) | 2020-07-30 |
US9625357B2 (en) | 2017-04-18 |
CN107367406B (zh) | 2020-03-06 |
WO2012120506A3 (en) | 2013-05-16 |
US10060836B2 (en) | 2018-08-28 |
EP3950136A1 (en) | 2022-02-09 |
WO2012120506A2 (en) | 2012-09-13 |
CN103608110A (zh) | 2014-02-26 |
CN107367406A (zh) | 2017-11-21 |
JP2017049256A (ja) | 2017-03-09 |
JP6310980B2 (ja) | 2018-04-11 |
US20180328821A1 (en) | 2018-11-15 |
JP2014511494A (ja) | 2014-05-15 |
US10983033B2 (en) | 2021-04-20 |
US20140033809A1 (en) | 2014-02-06 |
EP2683483B1 (en) | 2021-08-18 |
KR20140025380A (ko) | 2014-03-04 |
EP2683483A2 (en) | 2014-01-15 |
RU2013145080A (ru) | 2015-04-20 |
US20170146434A1 (en) | 2017-05-25 |
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