JP5887268B2 - ボツリヌス神経毒素を得るためのプロセスおよびシステム - Google Patents

ボツリヌス神経毒素を得るためのプロセスおよびシステム Download PDF

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JP5887268B2
JP5887268B2 JP2012520707A JP2012520707A JP5887268B2 JP 5887268 B2 JP5887268 B2 JP 5887268B2 JP 2012520707 A JP2012520707 A JP 2012520707A JP 2012520707 A JP2012520707 A JP 2012520707A JP 5887268 B2 JP5887268 B2 JP 5887268B2
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medium
botulinum
botulinum neurotoxin
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fermentation
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JP2012532932A (ja
JP2012532932A5 (enExample
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ジェニファー・エル・トン
ヘマント・エイ・ペイテル
ロナルド・シー・ベイツ
ワジディー・エム・アーマド
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Allergan Inc
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Allergan Inc
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/48Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00Medicinal preparations containing peptides
    • A61K38/16Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01DSEPARATION
    • B01D15/00Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08Selective adsorption, e.g. chromatography
    • B01D15/26Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/36Selective adsorption, e.g. chromatography characterised by the separation mechanism involving ionic interaction, e.g. ion-exchange, ion-pair, ion-suppression or ion-exclusion
    • B01D15/361Ion-exchange
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01DSEPARATION
    • B01D15/00Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08Selective adsorption, e.g. chromatography
    • B01D15/26Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/36Selective adsorption, e.g. chromatography characterised by the separation mechanism involving ionic interaction, e.g. ion-exchange, ion-pair, ion-suppression or ion-exclusion
    • B01D15/361Ion-exchange
    • B01D15/362Cation-exchange
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01DSEPARATION
    • B01D15/00Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08Selective adsorption, e.g. chromatography
    • B01D15/26Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/36Selective adsorption, e.g. chromatography characterised by the separation mechanism involving ionic interaction, e.g. ion-exchange, ion-pair, ion-suppression or ion-exclusion
    • B01D15/361Ion-exchange
    • B01D15/363Anion-exchange
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K1/00General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K1/00General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14Extraction; Separation; Purification
    • C07K1/16Extraction; Separation; Purification by chromatography
    • C07K1/18Ion-exchange chromatography
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K14/00Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/33Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Clostridium (G)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20Bacteria; Culture media therefor
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00Preparation of peptides or proteins
    • C12P21/02Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12RINDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00Microorganisms ; Processes using microorganisms
    • C12R2001/01Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/145Clostridium
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y304/00Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/24Metalloendopeptidases (3.4.24)
    • C12Y304/24069Bontoxilysin (3.4.24.69), i.e. botulinum neurotoxin
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A50/00TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
    • Y02A50/30Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Biotechnology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biophysics (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Biomedical Technology (AREA)
  • Virology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Epidemiology (AREA)
  • Immunology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Animal Behavior & Ethology (AREA)
  • Veterinary Medicine (AREA)
  • Public Health (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
JP2012520707A 2009-07-13 2010-07-12 ボツリヌス神経毒素を得るためのプロセスおよびシステム Active JP5887268B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US12/502,181 US8129139B2 (en) 2009-07-13 2009-07-13 Process for obtaining botulinum neurotoxin
US12/502,181 2009-07-13
PCT/US2010/041745 WO2011008713A1 (en) 2009-07-13 2010-07-12 Process and system for obtaining botulinum neurotoxin

Related Child Applications (1)

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JP2015214835A Division JP6356651B2 (ja) 2009-07-13 2015-10-30 ボツリヌス神経毒素を得るためのプロセスおよびシステム

Publications (3)

Publication Number Publication Date
JP2012532932A JP2012532932A (ja) 2012-12-20
JP2012532932A5 JP2012532932A5 (enExample) 2013-08-29
JP5887268B2 true JP5887268B2 (ja) 2016-03-16

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JP2012520707A Active JP5887268B2 (ja) 2009-07-13 2010-07-12 ボツリヌス神経毒素を得るためのプロセスおよびシステム
JP2015214835A Active JP6356651B2 (ja) 2009-07-13 2015-10-30 ボツリヌス神経毒素を得るためのプロセスおよびシステム
JP2016219708A Active JP6704329B2 (ja) 2009-07-13 2016-11-10 ボツリヌス神経毒素を得るためのプロセスおよびシステム
JP2020001854A Withdrawn JP2020078306A (ja) 2009-07-13 2020-01-09 ボツリヌス神経毒素を得るためのプロセスおよびシステム
JP2021142559A Pending JP2021193103A (ja) 2009-07-13 2021-09-01 ボツリヌス神経毒素を得るためのプロセスおよびシステム
JP2024098461A Pending JP2024111147A (ja) 2009-07-13 2024-06-19 ボツリヌス神経毒素を得るためのプロセスおよびシステム

Family Applications After (5)

Application Number Title Priority Date Filing Date
JP2015214835A Active JP6356651B2 (ja) 2009-07-13 2015-10-30 ボツリヌス神経毒素を得るためのプロセスおよびシステム
JP2016219708A Active JP6704329B2 (ja) 2009-07-13 2016-11-10 ボツリヌス神経毒素を得るためのプロセスおよびシステム
JP2020001854A Withdrawn JP2020078306A (ja) 2009-07-13 2020-01-09 ボツリヌス神経毒素を得るためのプロセスおよびシステム
JP2021142559A Pending JP2021193103A (ja) 2009-07-13 2021-09-01 ボツリヌス神経毒素を得るためのプロセスおよびシステム
JP2024098461A Pending JP2024111147A (ja) 2009-07-13 2024-06-19 ボツリヌス神経毒素を得るためのプロセスおよびシステム

Country Status (18)

Country Link
US (19) US8129139B2 (enExample)
EP (5) EP3252070B1 (enExample)
JP (6) JP5887268B2 (enExample)
KR (5) KR101916139B1 (enExample)
CN (2) CN102482332B (enExample)
BR (1) BR112012000784A2 (enExample)
CA (1) CA2767760C (enExample)
CY (2) CY1119519T1 (enExample)
DK (2) DK2454275T3 (enExample)
ES (2) ES2643554T3 (enExample)
HU (2) HUE033888T2 (enExample)
IN (1) IN2012DN00456A (enExample)
MX (4) MX2012000615A (enExample)
PL (2) PL3252070T3 (enExample)
PT (2) PT2454275T (enExample)
RU (1) RU2561459C2 (enExample)
SI (2) SI3252070T1 (enExample)
WO (1) WO2011008713A1 (enExample)

Cited By (1)

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JP2017070291A (ja) * 2009-07-13 2017-04-13 アラーガン、インコーポレイテッドAllergan,Incorporated ボツリヌス神経毒素を得るためのプロセスおよびシステム

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