JP5837420B2 - 固体又は半固体培地上の微生物のキャラクタリゼーション方法 - Google Patents
固体又は半固体培地上の微生物のキャラクタリゼーション方法 Download PDFInfo
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Description
本願は、2008年12月16日に出願した米国仮特許出願第61/122,925号「Methods for Characterization of Microorganisms on Solid or Semi Solid Media」の恩典を主張する。この米国仮特許出願の開示内容を本明細書に援用する。
これらのシステムでは16〜24時間かけて平板培養培地上で培養したコロニーが使用され、その後、これらのコロニーから標準懸濁液を作成し、実際のキャラクタリゼーション試験のために、更に4〜24時間かけて培養を完了することを要する。
(a)固体又は半固体培地上の1以上のコロニーを解析して、前記コロニー内の微生物の自家蛍光(IF)測定値特性を生成するステップと、
(b)前記自家蛍光(IF)測定値に基づいて前記コロニー内の前記微生物のキャラクタリゼーション及び/又は同定を行うステップとを含むことを特徴とする。
(a)1以上の微生物コロニーを含むことが既知である又は含む可能性がある培地を走査して前記培地上のコロニーを検出するステップと、
(b)前記ステップ(a)で検出した1以上のコロニーを解析して、前記コロニー内の微生物の自家蛍光(IF)測定値特性を生成するステップと、
(c)前記自家蛍光(IF)測定値に基づいて前記コロニー内の前記微生物の検出、キャラクタリゼーション、及び/又は同定を行うステップとを含むことを特徴とする。
(a)前記固体又は半固体培地上の試料に存在する微生物を培養して少なくとも1つのコロニーを生成するステップと、
(b)倍地上の1以上のコロニーを解析して、微生物の自家蛍光(IF)測定値特性を生成するステップと、
(c)生成された測定値に基づいて前記コロニー内の微生物をキャラクタリゼーション及び/又は同定するステップとを含むことを特徴とする。
(a)微生物を含むことが既知である又は含む可能性がある試料を取得するステップと、
(b)前記固体又は半固体培地上の試料に存在する微生物を培養するステップと、
(c)前記固体又は半固体培地をポイント−バイ−ポイント走査して自家蛍光(IF)測定値を生成して、前記培地上に存在するあらゆるコロニーを検出するステップであり、生成された前記測定値によって検出された1つ以上のコロニーの存在によって前記試料内に微生物が存在することが示唆されることを特徴とするステップと、を含むことを特徴とする。
本明細書で使用する「a」、「an」又は「the」は1つ又は2つ以上のものを意味する可能性がある。例えば「細胞(a cell)」という場合は単一の細胞を意味することも複数の細胞を意味することもある。
例えば、集束光をシステマチック若しくは所定のパターンで、又はランダムに表面上で移動させて、固体又は半固体培地を「走査」して、微生物コロニーを検出し、見つけ出し、さもなければ感知することができる。本実施形態によれば、光源は約0.5mm未満、約0.2mm未満、又は0.1mm未満の直径のビーム直径を有することが典型的である。他の実施形態では、ビーム直径は約5μm〜約500μm、約10μm〜約100μm、又は約20μm〜約80μmである。
(a)培地上の1以上のコロニーを解析して、コロニー内の微生物の自家蛍光(IF)測定値特性を生成するステップと、
(b)生成された測定値に基づいてコロニー内の微生物のキャラクタリゼーション及び/又は同定を行うステップとを含むことを特徴とする。
(a)1以上の微生物コロニーを含むことが既知である又は含む可能性がある培地を走査して培地上のコロニーを検出するステップと、
(b)ステップ(a)で検出した1以上のコロニーを解析して、コロニー内の微生物の自家蛍光(IF)測定値特性を生成するステップと、
(c)生成された測定値に基づいてコロニー内の微生物の検出、キャラクタリゼーション、及び/又は同定を行うステップとを含むことを特徴とする。
(a)固体又は半固体培地上の試料に存在する微生物を培養して少なくとも1つのコロニーを生成するステップと、
(b)倍地上の1以上のコロニーを解析して、微生物の自家蛍光(IF)測定値特性を生成するステップと、
(c)生成された測定値に基づいてコロニー内の微生物をキャラクタリゼーション及び/又は同定するステップとを含むことを特徴とする。
(a)微生物を含むことが既知である又は含む可能性がある試料を取得するステップと、
(b)固体又は半固体培地上の試料に存在する微生物を培養するステップと、
(c)培地を走査して自家蛍光(IF)測定値を生成して、培地上に存在するあらゆるコロニーを検出するステップであり、生成された測定値によって検出された1つ以上のコロニーの存在によって試料内に微生物が存在することが示唆されることを特徴とするステップと、を含むことを特徴とする。
フィルターを液体培地に曝した(例えば、浸した)材料(例えば、吸収パッド)の上に載置する。幾つかの実施形態では、試料(例えば、体積の大きい試料)をフィルターを通過させ、試料内に存在するあらゆる微生物を収集する。そして、フィルターを増殖培地の上面に載置して、適切な条件下で培養して微生物を増殖させることができる。適切なフィルター膜は、当業者によく知られており、微生物を収集することに適しており、且つ/又は、微生物の増殖をサポートすることができる、あらゆる膜を含む。膜材料は、例えば、セルロース、セルロース混合エステル、ニトロセルロース、ポリビニルクロライド、ナイロン、ポリテトラフルオロエチレン、ポリスルフォン、ポリエステルスルフォン、ポリカーボネートブラック、ブラック混合エステル(black mixed ester)、及びこれらのあらゆる組み合わせを含むが、これらに限定されない。フィルターは、液体をろ過するのに適し、且つ/又は、微生物の収集に適した細孔の大きさを有し、その大きさは例えば、酵母に対しては約1〜約25μmであり、細菌に対しては約0.05μm〜約2μm、例えば、0.2μm〜約1μmである。
プレート及び膜上のコロニーからのスペクトルの取得
無し=ヒツジ血液観点(SBA)
ポール=ポール社製グリッド付きメトリセルブラックポリエーテルスルホンメンブレン(SBA上)
WPC=ワットマン社製トラックエッチポリカーボネートブラックメンブレン(SBA上)
WME=ワットマン社製ブラック混合エステルメンブレン(SBA上)
顕微鏡によるマイクロコロニーの走査
寒天プレート上の微生物コロニーの分類
ノイズの少ないコロニーの分類の改良
Claims (9)
- 固体又は半固体培地上の微生物を検出及び同定する方法であって、
(a)1以上の微生物コロニーを含むことが既知である又は含む可能性がある固体又は半固体培地を走査して前記培地上のあらゆるコロニーを検出するステップと、
(b)前記ステップ(a)で検出した1以上のコロニーを、1000μmより小さい直径を有する励起ビームによって、直接、解析して、前記コロニー内の微生物の自家蛍光(IF)測定値特性を生成するステップと、
(c)前記自家蛍光(IF)測定値に基づき、励起発光マトリクス(EEM)を判定するステップを含み、該EEMは少なくとも2つの異なる波長ペアを含み、
(d)前記EEMを既知の微生物のEEMのデータベースと分類モデリング分析によって比較するステップを含み、
(e)前記分類モデリング分析に基づいて前記コロニー内の前記微生物の同定を行うステップとを含み、該同定は、科、属、種、及び/又は株を決定することを意味することを特徴とする方法。 - 請求項1に記載の方法であり、前記走査は、前記固体又は半固体培地の表面のポイント−バイ−ポイント走査であることを特徴とする、方法。
- 請求項1に記載の方法であり、前記コロニーは直径50μm未満のマイクロコロニーであることを特徴とする、方法。
- 請求項1に記載の方法であり、解析する前記ステップは非侵襲的であることを特徴とする、方法。
- 請求項1に記載の方法であり、前記培地又は試料に対して微生物を種に同定するための同定作用物質を添加するステップを更に含み、コロニー又は該コロニーから回収した微生物内の前記同定作用物質の有無に部分的に基づいて同定を行い、
前記同定作用物質は、親和性リガンドであることを特徴とする、方法。 - 請求項1に記載の方法であり、前記固体又は半固体培地は1種以上の前記微生物の成長に有益な栄養素及び1種以上の添加物を含有し、前記1種以上の添加物は前記固体又は半固体培地上の前記微生物コロニーの自家蛍光測定値を強化することを特徴とする、方法。
- 請求項6に記載の方法であり、前記1種以上の添加物は、タンパク質加水分解物、アミノ酸、肉及び野菜抽出物、炭水化物源、緩衝剤、蘇生剤、増殖因子、酵素補助因子、無機塩類、補助金属、還元化合物、キレート剤、感光剤、消光剤、還元剤、酸化剤、洗剤、界面活性剤、殺菌剤、選択剤、代謝阻害剤を含む群から選択されることを特徴とする、方法。
- 請求項1に記載の方法であり、前記コロニーの解析は、エピ蛍光の測定ステップを含むことを特徴とする、方法。
- 請求項1に記載の方法であり、前記コロニーの解析は、反射光を測定するステップを含むことを特徴とする、方法。
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Families Citing this family (31)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US8512975B2 (en) * | 2008-07-24 | 2013-08-20 | Biomerieux, Inc. | Method for detection and characterization of a microorganism in a sample using time dependent spectroscopic measurements |
FR2951548B1 (fr) * | 2009-10-15 | 2011-11-11 | Biomerieux Sa | Procede de caracterisation d'au moins un microorganisme par spectrometrie de masse |
EP2795293A4 (en) * | 2011-12-19 | 2016-01-20 | Opticul Diagnostics Ltd | SPECTROSCOPIC DEVICE AND METHOD FOR IDENTIFYING MICROORGANISMS IN CULTURES |
EP2648133A1 (fr) * | 2012-04-04 | 2013-10-09 | Biomerieux | Identification de microorganismes par spectrometrie et classification structurée |
CN105378060B (zh) | 2013-03-13 | 2019-12-10 | 经纬生物科技有限公司 | 非复制型转导颗粒和基于转导颗粒的报告系统 |
US9481903B2 (en) | 2013-03-13 | 2016-11-01 | Roche Molecular Systems, Inc. | Systems and methods for detection of cells using engineered transduction particles |
US20160075988A1 (en) | 2013-05-06 | 2016-03-17 | 3M Innovative Properties Company | Culture device and methods for enumerating mold colonies |
JP2015031517A (ja) * | 2013-07-31 | 2015-02-16 | 浜松ホトニクス株式会社 | 植物体の病原菌感染診断方法及び病原菌感染診断装置 |
US9540675B2 (en) | 2013-10-29 | 2017-01-10 | GeneWeave Biosciences, Inc. | Reagent cartridge and methods for detection of cells |
FR3030748B1 (fr) * | 2014-12-17 | 2017-01-27 | Commissariat Energie Atomique | Systeme d'observation d'objets |
EP3265941A4 (en) * | 2015-03-06 | 2018-08-15 | Pocared Diagnostics Ltd. | Reagent-free identification of bacteria containing resistance genes using a rapid intrinsic fluorescence method |
KR101907194B1 (ko) * | 2015-03-24 | 2018-10-16 | 주식회사 기술과창조 | 복합 미생물 종균을 이용하여 제조된 사료 첨가제 및 그 제조 방법 |
US10351893B2 (en) | 2015-10-05 | 2019-07-16 | GeneWeave Biosciences, Inc. | Reagent cartridge for detection of cells |
ES2716170T3 (es) * | 2016-06-16 | 2019-06-10 | Biomerieux Sa | Método y sistema de identificación del tipo de Gram de una bacteria |
US10738338B2 (en) | 2016-10-18 | 2020-08-11 | The Research Foundation for the State University | Method and composition for biocatalytic protein-oligonucleotide conjugation and protein-oligonucleotide conjugate |
JP6422616B1 (ja) * | 2016-12-22 | 2018-11-14 | 国立大学法人 筑波大学 | データ作成方法及びデータ使用方法 |
US11867892B2 (en) | 2017-07-06 | 2024-01-09 | The Johns Hopkins University | Miniature microscope for multi-contrast optical imaging in animals |
JP7313332B2 (ja) | 2017-07-27 | 2023-07-24 | ビオメリュー・インコーポレイテッド | 隔離チューブ |
WO2019070975A1 (en) * | 2017-10-05 | 2019-04-11 | Becton Dickinson And Company | ENVIRONMENT FOR DEVELOPING APPLICATIONS FOR EVALUATION PROCESSING OF BIOLOGICAL SAMPLES |
FR3075825B1 (fr) | 2017-12-21 | 2022-01-21 | Biomerieux Sa | Procede et systeme d'identificationdu type de gram d'une bacterie |
FR3075824B1 (fr) * | 2017-12-21 | 2022-01-14 | Biomerieux Sa | Procede d'identification d'une levure ou d'une bacterie |
FR3082523B1 (fr) * | 2018-06-18 | 2022-11-18 | Maat Pharma | Procede de detection des bacteries selon leur signal gram dans un echantillon complexe |
CN108872573B (zh) * | 2018-07-04 | 2021-04-30 | 中国水产科学研究院东海水产研究所 | 基于纳米磁珠-荧光激团的单增李斯特菌快速检测方法 |
WO2021092110A1 (en) * | 2019-11-05 | 2021-05-14 | Nuclease Probe Technologies, Inc. | Microbial detection platform |
EP4065958A1 (en) * | 2019-11-29 | 2022-10-05 | Intubio ApS | A method and a system for analysing a fluid sample for a biological activity |
FR3103900B1 (fr) * | 2019-11-29 | 2024-07-19 | Univ Du Mans | Méthode d'identification rapide de microorganismes par analyse de matrices excitation-émission |
DE102020105123B3 (de) * | 2020-02-27 | 2021-07-01 | Bruker Daltonik Gmbh | Verfahren zum spektrometrischen Charakterisieren von Mikroorganismen |
WO2021258223A1 (en) * | 2020-06-25 | 2021-12-30 | Qvella Corporation | Systems and methods for classification of microbial cells grown in microcolonies |
PL245363B1 (pl) * | 2021-01-13 | 2024-07-08 | Ml System Spolka Akcyjna | Urządzenie i sposób do nieinwazyjnego wykrywania i identyfikacji mikroorganizmów w próbkach z materiałów stałych, ciekłych i w próbkach gazowych |
CN113493815A (zh) * | 2021-03-18 | 2021-10-12 | 甘肃国信润达分析测试中心 | 一种水质中微生物的测定方法 |
CN118392845B (zh) * | 2024-06-25 | 2024-10-01 | 中国科学院过程工程研究所 | 一种用于对空气浮游微生物进行监测鉴别的复合功能多孔金属膜及检测系统、以及空气浮游微生物监测鉴别方法 |
Family Cites Families (38)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN85100424B (zh) | 1985-04-01 | 1986-10-29 | 上海医疗器械研究所 | 恶性肿瘤固有荧光诊断仪 |
US4847198A (en) | 1987-10-07 | 1989-07-11 | The Board Of Governors For Higher Education, State Of Rhode Island And Providence Plantations | Detection and indentification of bacteria by means of ultra-violet excited resonance Raman spectra |
DE3903777A1 (de) | 1989-02-09 | 1990-08-16 | Bruker Analytische Messtechnik | Verfahren zur schnellen detektion von mikroorganismen in proben und vorrichtung zur durchfuehrung des verfahrens |
US5112745A (en) | 1989-09-29 | 1992-05-12 | Space Medical Systems, Inc. | Rapid identification of microbial organisms and determination of antibiotic sensitivity by infrared spectroscopy |
US6497872B1 (en) * | 1991-07-08 | 2002-12-24 | Neurospheres Holdings Ltd. | Neural transplantation using proliferated multipotent neural stem cells and their progeny |
US5474910A (en) | 1993-10-15 | 1995-12-12 | Alfano; Robert R. | Method and device for detecting biological molecules and/or microorganisms within a desired area or space |
US5599717A (en) | 1994-09-02 | 1997-02-04 | Martin Marietta Energy Systems, Inc. | Advanced synchronous luminescence system |
DE69417900T2 (de) | 1994-11-17 | 1999-11-11 | Chemunex, Maisons-Alfort | Vorrichtung und Verfahren zum schnellen und hochempfindlichen Erkennen und Zählen von Mikroorganismen mittels Fluoreszenz |
JPH08145796A (ja) * | 1994-11-25 | 1996-06-07 | Hitachi Ltd | 三次元スペクトル表示装置 |
US5912115A (en) | 1997-12-12 | 1999-06-15 | Akzo Nobel, N.V. | Evacuated sensor device for detecting microorganisms in blood samples, and method thereof |
US6571118B1 (en) | 1998-05-04 | 2003-05-27 | Board Of Regents, The University Of Texas System | Combined fluorescence and reflectance spectroscopy |
US6153400A (en) | 1999-03-12 | 2000-11-28 | Akzo Nobel N.V. | Device and method for microbial antibiotic susceptibility testing |
US6251624B1 (en) | 1999-03-12 | 2001-06-26 | Akzo Nobel N.V. | Apparatus and method for detecting, quantifying and characterizing microorganisms |
US20020086289A1 (en) | 1999-06-15 | 2002-07-04 | Don Straus | Genomic profiling: a rapid method for testing a complex biological sample for the presence of many types of organisms |
EP1290428A1 (en) | 2000-06-02 | 2003-03-12 | Medicometrics APS | Method and system for classifying a biological sample |
AU2001288762A1 (en) | 2000-09-08 | 2002-03-22 | Large Scale Proteomics Corporation | Detection and characterization of microorganisms |
EP1428018B1 (en) * | 2001-09-06 | 2010-06-09 | Straus Holdings Inc. | Rapid and sensitive detection of molecules |
US6780602B2 (en) | 2001-11-01 | 2004-08-24 | Microbiosystems, Limited Partnership | Taxonomic identification of pathogenic microorganisms and their toxic proteins |
AU2002346615B2 (en) | 2001-12-03 | 2008-04-17 | Seroptix, Inc. | A method for identifying markers |
US7428045B2 (en) | 2002-01-10 | 2008-09-23 | Chemimage Corporation | Raman spectral analysis of pathogens |
EP1463926A4 (en) * | 2002-01-10 | 2010-09-08 | Chemimage Corp | METHOD FOR DETECTING PATHOGENIC MICROORGANISMS |
US6750006B2 (en) * | 2002-01-22 | 2004-06-15 | Microbiosystems, Limited Partnership | Method for detecting the presence of microbes and determining their physiological status |
US7186990B2 (en) * | 2002-01-22 | 2007-03-06 | Microbiosystems, Limited Partnership | Method and apparatus for detecting and imaging the presence of biological materials |
EP1623212A1 (en) | 2003-05-12 | 2006-02-08 | Erasmus University Medical Center Rotterdam | Automated characterization and classification of microorganisms |
US20060257929A1 (en) | 2003-11-12 | 2006-11-16 | Microbiosystems, Limited Partnership | Method for the rapid taxonomic identification of pathogenic microorganisms and their toxic proteins |
US7429464B2 (en) | 2003-12-09 | 2008-09-30 | Biomerieux, Inc. | Methods for detecting bacterial pathogens |
US7824883B2 (en) | 2003-12-31 | 2010-11-02 | Powers Linda S | Method and apparatus for detecting the presence of microbes with frequency modulated multi-wavelength intrinsic fluorescence |
US7518710B2 (en) | 2005-07-14 | 2009-04-14 | Battelle Memorial Institute | Optical devices for biological and chemical detection |
US20070037135A1 (en) * | 2005-08-08 | 2007-02-15 | Barnes Russell H | System and method for the identification and quantification of a biological sample suspended in a liquid |
US20070111225A1 (en) * | 2005-08-10 | 2007-05-17 | California Institute Of Technology | System and method for monitoring an analyte |
NZ542230A (en) | 2005-09-05 | 2008-05-30 | Veritide Ltd | System for spore detection |
KR101418295B1 (ko) | 2006-06-27 | 2014-07-10 | 아즈빌 바이오비질런트, 인크. | 크기/형광의 동시적 측정에 의한 병원체 검출 |
WO2009011565A1 (en) | 2007-07-17 | 2009-01-22 | Erasmus University Medical Center Rotterdam | Method for typing and identification of micro-organisms |
US8280471B2 (en) | 2007-12-12 | 2012-10-02 | Kimberly-Clark Worldwide, Inc. | Fiber optic based detection of autofluorescent bacterial pathogens |
JP5608159B2 (ja) | 2008-03-26 | 2014-10-15 | スリーエム イノベイティブ プロパティズ カンパニー | 生物学的増殖培地のスペクトル解析 |
TR201901658T4 (tr) | 2008-05-20 | 2019-02-21 | Univ Health Network | Floresan bazli görüntüleme ve i̇zleme i̇çi̇n ci̇haz ve metot |
CN101498666A (zh) | 2008-09-22 | 2009-08-05 | 中国海洋大学 | 荧光法快速测定活细菌总数 |
MX2011003982A (es) * | 2008-10-31 | 2011-09-21 | Bio Merieux Inc | Métodos para la separación, caracterización y/o identificación de microorganismos usando espectroscopia. |
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KR101650017B1 (ko) | 2016-08-23 |
AU2009333848A1 (en) | 2011-06-30 |
WO2010077304A3 (en) | 2010-09-30 |
EP2376914A2 (en) | 2011-10-19 |
WO2010077304A2 (en) | 2010-07-08 |
CA2745872C (en) | 2019-04-02 |
BRPI0922952A2 (pt) | 2016-01-19 |
MX2011005988A (es) | 2011-09-01 |
AU2009333848B2 (en) | 2016-02-18 |
KR20110106373A (ko) | 2011-09-28 |
US20110033847A1 (en) | 2011-02-10 |
US9822389B2 (en) | 2017-11-21 |
CN102317777B (zh) | 2015-01-07 |
CN104502317B (zh) | 2017-10-17 |
RU2011123155A (ru) | 2013-01-27 |
JP2012511905A (ja) | 2012-05-31 |
CN102317777A (zh) | 2012-01-11 |
EP2376914B1 (en) | 2017-04-19 |
RU2523903C2 (ru) | 2014-07-27 |
JP6186414B2 (ja) | 2017-08-23 |
CN104502317A (zh) | 2015-04-08 |
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