JP5777113B2 - 人工多能性幹細胞のクローンの選択方法 - Google Patents

人工多能性幹細胞のクローンの選択方法 Download PDF

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JP5777113B2
JP5777113B2 JP2012512547A JP2012512547A JP5777113B2 JP 5777113 B2 JP5777113 B2 JP 5777113B2 JP 2012512547 A JP2012512547 A JP 2012512547A JP 2012512547 A JP2012512547 A JP 2012512547A JP 5777113 B2 JP5777113 B2 JP 5777113B2
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JP2012527887A (ja
JP2012527887A5 (enExample
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岡野　栄之
栄之 岡野
洋平 岡田
洋平 岡田
伸弥 山中
伸弥 山中
恭子 三浦
恭子 三浦
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Keio University
Kyoto University NUC
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    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6881—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for tissue or cell typing, e.g. human leukocyte antigen [HLA] probes
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0618—Cells of the nervous system
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0696—Artificially induced pluripotent stem cells, e.g. iPS
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/115—Basic fibroblast growth factor (bFGF, FGF-2)
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/30—Hormones
    • C12N2501/38—Hormones with nuclear receptors
    • C12N2501/385—Hormones with nuclear receptors of the family of the retinoic acid recptor, e.g. RAR, RXR; Peroxisome proliferator-activated receptor [PPAR]
    • C—CHEMISTRY; METALLURGY
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/60—Transcription factors
    • C12N2501/606—Transcription factors c-Myc
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/45—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from artificially induced pluripotent stem cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2510/00—Genetically modified cells
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/158—Expression markers

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  • Health & Medical Sciences (AREA)
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  • Engineering & Computer Science (AREA)
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  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
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  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
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  • Immunology (AREA)
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  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Transplantation (AREA)
  • Neurosurgery (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
JP2012512547A 2009-05-29 2010-05-28 人工多能性幹細胞のクローンの選択方法 Expired - Fee Related JP5777113B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US21736209P 2009-05-29 2009-05-29
US61/217,362 2009-05-29
PCT/JP2010/003620 WO2010137348A1 (en) 2009-05-29 2010-05-28 Method for selecting clone of induced pluripotent stem cells

Publications (3)

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JP2012527887A JP2012527887A (ja) 2012-11-12
JP2012527887A5 JP2012527887A5 (enExample) 2013-08-08
JP5777113B2 true JP5777113B2 (ja) 2015-09-09

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US (1) US9005975B2 (enExample)
EP (1) EP2438159B1 (enExample)
JP (1) JP5777113B2 (enExample)
WO (1) WO2010137348A1 (enExample)

Families Citing this family (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SG10202103401QA (en) * 2008-03-17 2021-05-28 Scripps Research Inst Combined chemical and genetic approaches for generation of induced pluripotent stem cells
JP5671737B2 (ja) * 2008-08-05 2015-02-18 学校法人慶應義塾 分化細胞由来多能性幹細胞由来の二次ニューロスフェアの選択方法、その選択方法によって選択されたクローン、及びそのクローンの使用方法
WO2010077955A1 (en) 2008-12-17 2010-07-08 The Scripps Research Institute Generation and maintenance of stem cells
WO2011027908A1 (en) 2009-09-02 2011-03-10 Kyoto University Method of selecting safe pluripotent stem cells
ES2938049T3 (es) 2009-10-16 2023-04-04 Scripps Research Inst Inducción de células pluripotentes
WO2011056971A2 (en) 2009-11-04 2011-05-12 Cellular Dynamics International, Inc. Episomal reprogramming with chemicals
AU2011235212B2 (en) 2010-03-31 2014-07-31 The Scripps Research Institute Reprogramming cells
EP3399026B1 (en) 2010-06-14 2024-06-26 The Scripps Research Institute Reprogramming of cells to a new fate
JP5936134B2 (ja) 2010-06-15 2016-06-15 国立大学法人京都大学 ヒト人工多能性幹細胞の選択方法
KR102703637B1 (ko) 2010-12-22 2024-09-05 페이트 세러퓨틱스, 인코포레이티드 단세포 분류 및 iPSC의 증강된 재프로그래밍을 위한 세포 배양 플랫폼
KR101791926B1 (ko) * 2011-07-25 2017-11-20 고쿠리츠 다이가쿠 호진 교토 다이가쿠 유도 만능 줄기 세포 스크리닝 방법
US20170051306A1 (en) * 2014-01-14 2017-02-23 Kagoshima University Novel method for labelling cancerization-causing cell in stem cells, and therapy method
KR102340553B1 (ko) 2014-03-04 2021-12-21 페이트 세러퓨틱스, 인코포레이티드 개선된 재프로그래밍 방법 및 세포 배양 플랫폼
AU2016338680B2 (en) 2015-10-16 2022-11-17 Fate Therapeutics, Inc. Platform for the induction and maintenance of ground state pluripotency
KR101783977B1 (ko) 2016-12-30 2017-10-10 제일약품주식회사 기형종 형성이 억제된 전분화능 줄기세포 유래 신경 전구체구의 제조 방법
WO2018232079A1 (en) 2017-06-14 2018-12-20 Daley George Q Hematopoietic stem and progenitor cells derived from hemogenic endothelial cells by episomal plasmid gene transfer
WO2021150919A1 (en) 2020-01-23 2021-07-29 The Children's Medical Center Corporation Stroma-free t cell differentiation from human pluripotent stem cells

Family Cites Families (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP3660601B2 (ja) 2001-03-30 2005-06-15 独立行政法人科学技術振興機構 胚性幹細胞からの神経幹細胞、運動ニューロン及びｇａｂａ作動性ニューロンの製造法
AUPS225402A0 (en) 2002-05-10 2002-06-13 Bresagen Limited Modified cell production
JP4901471B2 (ja) 2004-02-19 2012-03-21 国立大学法人京都大学 体細胞核初期化物質のスクリーニング方法
AU2006304390A1 (en) 2005-10-17 2007-04-26 Academia Sinica Pulmonary stem cells, related methods and kits
US8129187B2 (en) * 2005-12-13 2012-03-06 Kyoto University Somatic cell reprogramming by retroviral vectors encoding Oct3/4. Klf4, c-Myc and Sox2
US8278104B2 (en) * 2005-12-13 2012-10-02 Kyoto University Induced pluripotent stem cells produced with Oct3/4, Klf4 and Sox2
US8048999B2 (en) 2005-12-13 2011-11-01 Kyoto University Nuclear reprogramming factor
KR100794362B1 (ko) 2006-02-27 2008-01-15 주식회사 임젠 Ｎａｎｏｇ를 이용한 아스트로사이트의 신경줄기세포로의역분화
EP2137296A2 (en) 2007-03-23 2009-12-30 Wisconsin Alumni Research Foundation Somatic cell reprogramming
CA2660123C (en) 2007-10-31 2017-05-09 Kyoto University Nuclear reprogramming method
EP2072618A1 (en) * 2007-12-14 2009-06-24 Johannes Gutenberg-Universität Mainz Use of RNA for reprogramming somatic cells
SG10202103401QA (en) 2008-03-17 2021-05-28 Scripps Research Inst Combined chemical and genetic approaches for generation of induced pluripotent stem cells
JP5671737B2 (ja) 2008-08-05 2015-02-18 学校法人慶應義塾 分化細胞由来多能性幹細胞由来の二次ニューロスフェアの選択方法、その選択方法によって選択されたクローン、及びそのクローンの使用方法
JP5936134B2 (ja) 2010-06-15 2016-06-15 国立大学法人京都大学 ヒト人工多能性幹細胞の選択方法

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Publication number Publication date
WO2010137348A1 (en) 2010-12-02
JP2012527887A (ja) 2012-11-12
US20120129172A1 (en) 2012-05-24
US9005975B2 (en) 2015-04-14
EP2438159A1 (en) 2012-04-11
EP2438159B1 (en) 2018-10-03
EP2438159A4 (en) 2015-09-09

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