JP5715158B2 - 磁性ガラス粒子からの核酸の改善された回収 - Google Patents

磁性ガラス粒子からの核酸の改善された回収 Download PDF

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JP5715158B2
JP5715158B2 JP2012547490A JP2012547490A JP5715158B2 JP 5715158 B2 JP5715158 B2 JP 5715158B2 JP 2012547490 A JP2012547490 A JP 2012547490A JP 2012547490 A JP2012547490 A JP 2012547490A JP 5715158 B2 JP5715158 B2 JP 5715158B2
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solid phase
nucleic acid
nucleic acids
ethylene
sample
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Japanese (ja)
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JP2013516185A (ja
JP2013516185A5 (https=
Inventor
エー．ジョンソン ジェニー
エー．ジョンソン ジェニー
カイガー エリッチ
カイガー エリッチ
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F Hoffmann La Roche AG
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F Hoffmann La Roche AG
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C12N15/1013—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by using magnetic beads
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • Analytical Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Immunology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Solid-Sorbent Or Filter-Aiding Compositions (AREA)
  • Indole Compounds (AREA)
  • Saccharide Compounds (AREA)
JP2012547490A 2010-01-08 2011-01-05 磁性ガラス粒子からの核酸の改善された回収 Active JP5715158B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US12/684,762 US8420801B2 (en) 2010-01-08 2010-01-08 Recovery of nucleic acids from magnetic glass particles
US12/684,762 2010-01-08
PCT/EP2011/000013 WO2011083076A1 (en) 2010-01-08 2011-01-05 Improved recovery of nucleic acids from magnetic glass particles

Publications (3)

Publication Number Publication Date
JP2013516185A JP2013516185A (ja) 2013-05-13
JP2013516185A5 JP2013516185A5 (https=) 2013-10-03
JP5715158B2 true JP5715158B2 (ja) 2015-05-07

Family

ID=43639930

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2012547490A Active JP5715158B2 (ja) 2010-01-08 2011-01-05 磁性ガラス粒子からの核酸の改善された回収

Country Status (7)

Country Link
US (1) US8420801B2 (https=)
EP (1) EP2521779B1 (https=)
JP (1) JP5715158B2 (https=)
CN (1) CN102834518B (https=)
CA (1) CA2786644C (https=)
ES (1) ES2530466T3 (https=)
WO (1) WO2011083076A1 (https=)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20140087366A1 (en) * 2012-09-19 2014-03-27 Beckman Coulter, Inc. USE OF DIVALENT IONS, PROTEASES, DETERGENTS, AND LOW pH IN THE EXTRACTION OF NUCLEIC ACIDS
JP2017539080A (ja) 2014-10-23 2017-12-28 コーニング インコーポレイテッド 高分子被包磁性ナノ粒子
WO2018061877A1 (ja) * 2016-09-30 2018-04-05 栄研化学株式会社 核酸を抽出する方法及びそれに用いるキット
EP3681895A4 (en) 2017-09-13 2021-05-26 Becton, Dickinson and Company METHODS AND COMPOSITIONS FOR EXTRACTION OF NUCLEIC ACIDS USING FERROUS PARTICLES
CN107955810A (zh) * 2017-12-25 2018-04-24 宁波卡尔新材料科技有限公司 一种新型动物肝脏核酸提取试剂盒及提取方法
US12344884B2 (en) 2018-06-28 2025-07-01 Gen-Probe Incorporated Sample preparation method and system

Family Cites Families (20)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5185243A (en) * 1988-08-25 1993-02-09 Syntex (U.S.A.) Inc. Method for detection of specific nucleic acid sequences
US5573907A (en) * 1990-01-26 1996-11-12 Abbott Laboratories Detecting and amplifying target nucleic acids using exonucleolytic activity
WO1995021271A1 (en) * 1994-02-07 1995-08-10 Molecular Tool, Inc. Ligase/polymerase-mediated genetic bit analysistm of single nucleotide polymorphisms and its use in genetic analysis
DE19520398B4 (de) 1995-06-08 2009-04-16 Roche Diagnostics Gmbh Magnetisches Pigment
EP1154443A1 (en) 2000-05-12 2001-11-14 Boehringer Mannheim Gmbh Magnetic glass particles, method for their preparation and uses thereof
WO1999003883A1 (en) * 1997-07-18 1999-01-28 Brigham And Women's Hospital Compositions and methods based upon the tuberous sclerosis-1 (tsc1) gene and gene product
WO1999058664A1 (en) 1998-05-14 1999-11-18 Whitehead Institute For Biomedical Research Solid phase technique for selectively isolating nucleic acids
AU3902799A (en) 1998-05-14 1999-11-29 University Of Hawaii Compositions and methods for genetic transformation of pineapple
DE60015148T2 (de) * 1999-08-20 2006-02-16 Promega Corp., Madison Simultane isolierung und quantifizierung von dna
US6548256B2 (en) 2000-07-14 2003-04-15 Eppendorf 5 Prime, Inc. DNA isolation method and kit
WO2003057910A2 (en) * 2002-01-08 2003-07-17 Roche Diagnostics Gmbh Use of silica material in an amplification reaction
GB0217963D0 (en) 2002-08-02 2002-09-11 Cyclops Genome Sciences Ltd Purification of nucleic acids
EP1560926B2 (de) * 2002-11-08 2013-08-21 STRATEC Molecular GmbH Neuartige pufferformulierungen zur isolierung, reinigung und rückgewinnung lang- und kurzkettiger nukleinsäuren
US20060240430A1 (en) * 2002-12-04 2006-10-26 Ying Wu Method for hybridisation of immobilized genomic dna
CN1970752A (zh) * 2006-11-29 2007-05-30 吉林大学 用磁性纳米复合材料回收dna的方法及试剂盒
CN105441549A (zh) 2007-03-30 2016-03-30 霍夫曼-拉罗奇有限公司 烷基胺改进甲醛固定的生物样品组分的检测
CA2629589C (en) 2007-04-20 2016-03-29 F.Hoffmann-La Roche Ag Isolation and purification of nucleic acid molecules with a solid phase
JP5292722B2 (ja) * 2007-05-09 2013-09-18 凸版印刷株式会社 未精製血液をサンプルとする核酸増幅法
EP2157181A1 (de) 2008-08-13 2010-02-24 AGOWA Gesellschaft für molekularbiologische Technologie mbH Verfahren zur Isolation von Nukleinsäuren und Testkit
AT508355B1 (de) 2009-06-29 2011-01-15 Trumpf Maschinen Austria Gmbh Verfahren und vorrichtung zum biegen eines werkstücks

Also Published As

Publication number Publication date
JP2013516185A (ja) 2013-05-13
CA2786644A1 (en) 2011-07-14
US8420801B2 (en) 2013-04-16
CA2786644C (en) 2015-08-11
US20110172408A1 (en) 2011-07-14
EP2521779B1 (en) 2014-12-10
CN102834518B (zh) 2015-04-01
WO2011083076A1 (en) 2011-07-14
CN102834518A (zh) 2012-12-19
WO2011083076A8 (en) 2014-08-07
ES2530466T3 (es) 2015-03-02
EP2521779A1 (en) 2012-11-14

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