JP2013516185A5 - - Google Patents
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- Publication number
- JP2013516185A5 JP2013516185A5 JP2012547490A JP2012547490A JP2013516185A5 JP 2013516185 A5 JP2013516185 A5 JP 2013516185A5 JP 2012547490 A JP2012547490 A JP 2012547490A JP 2012547490 A JP2012547490 A JP 2012547490A JP 2013516185 A5 JP2013516185 A5 JP 2013516185A5
- Authority
- JP
- Japan
- Prior art keywords
- solid phase
- ethylene
- amine compound
- reagent
- nucleic acid
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 239000007790 solid phase Substances 0.000 claims 12
- 150000007523 nucleic acids Chemical class 0.000 claims 10
- 102000039446 nucleic acids Human genes 0.000 claims 10
- 108020004707 nucleic acids Proteins 0.000 claims 10
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims 6
- 239000003153 chemical reaction reagent Substances 0.000 claims 6
- -1 ethylene-amine compound Chemical class 0.000 claims 6
- 238000000034 method Methods 0.000 claims 4
- 239000011541 reaction mixture Substances 0.000 claims 4
- 239000012488 sample solution Substances 0.000 claims 4
- 230000009089 cytolysis Effects 0.000 claims 3
- 239000002245 particle Substances 0.000 claims 3
- 239000000377 silicon dioxide Substances 0.000 claims 3
- JLVVSXFLKOJNIY-UHFFFAOYSA-N Magnesium ion Chemical compound [Mg+2] JLVVSXFLKOJNIY-UHFFFAOYSA-N 0.000 claims 2
- 241000186359 Mycobacterium Species 0.000 claims 2
- 150000008044 alkali metal hydroxides Chemical class 0.000 claims 2
- 239000003599 detergent Substances 0.000 claims 2
- 229910001425 magnesium ion Inorganic materials 0.000 claims 2
- 229910001437 manganese ion Inorganic materials 0.000 claims 2
- 239000000523 sample Substances 0.000 claims 2
- 241000193830 Bacillus <bacterium> Species 0.000 claims 1
- 241000894006 Bacteria Species 0.000 claims 1
- 241000193403 Clostridium Species 0.000 claims 1
- 241000194033 Enterococcus Species 0.000 claims 1
- 241000191940 Staphylococcus Species 0.000 claims 1
- 241000194017 Streptococcus Species 0.000 claims 1
- 238000010828 elution Methods 0.000 claims 1
- UYMKPFRHYYNDTL-UHFFFAOYSA-N ethenamine Chemical class NC=C UYMKPFRHYYNDTL-UHFFFAOYSA-N 0.000 claims 1
- 229910021645 metal ion Inorganic materials 0.000 claims 1
- 239000000203 mixture Substances 0.000 claims 1
- 238000006386 neutralization reaction Methods 0.000 claims 1
- 238000005406 washing Methods 0.000 claims 1
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US12/684,762 US8420801B2 (en) | 2010-01-08 | 2010-01-08 | Recovery of nucleic acids from magnetic glass particles |
| US12/684,762 | 2010-01-08 | ||
| PCT/EP2011/000013 WO2011083076A1 (en) | 2010-01-08 | 2011-01-05 | Improved recovery of nucleic acids from magnetic glass particles |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2013516185A JP2013516185A (ja) | 2013-05-13 |
| JP2013516185A5 true JP2013516185A5 (https=) | 2013-10-03 |
| JP5715158B2 JP5715158B2 (ja) | 2015-05-07 |
Family
ID=43639930
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2012547490A Active JP5715158B2 (ja) | 2010-01-08 | 2011-01-05 | 磁性ガラス粒子からの核酸の改善された回収 |
Country Status (7)
| Country | Link |
|---|---|
| US (1) | US8420801B2 (https=) |
| EP (1) | EP2521779B1 (https=) |
| JP (1) | JP5715158B2 (https=) |
| CN (1) | CN102834518B (https=) |
| CA (1) | CA2786644C (https=) |
| ES (1) | ES2530466T3 (https=) |
| WO (1) | WO2011083076A1 (https=) |
Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20140087366A1 (en) * | 2012-09-19 | 2014-03-27 | Beckman Coulter, Inc. | USE OF DIVALENT IONS, PROTEASES, DETERGENTS, AND LOW pH IN THE EXTRACTION OF NUCLEIC ACIDS |
| JP2017539080A (ja) | 2014-10-23 | 2017-12-28 | コーニング インコーポレイテッド | 高分子被包磁性ナノ粒子 |
| WO2018061877A1 (ja) * | 2016-09-30 | 2018-04-05 | 栄研化学株式会社 | 核酸を抽出する方法及びそれに用いるキット |
| EP3681895A4 (en) | 2017-09-13 | 2021-05-26 | Becton, Dickinson and Company | METHODS AND COMPOSITIONS FOR EXTRACTION OF NUCLEIC ACIDS USING FERROUS PARTICLES |
| CN107955810A (zh) * | 2017-12-25 | 2018-04-24 | 宁波卡尔新材料科技有限公司 | 一种新型动物肝脏核酸提取试剂盒及提取方法 |
| US12344884B2 (en) | 2018-06-28 | 2025-07-01 | Gen-Probe Incorporated | Sample preparation method and system |
Family Cites Families (20)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5185243A (en) * | 1988-08-25 | 1993-02-09 | Syntex (U.S.A.) Inc. | Method for detection of specific nucleic acid sequences |
| US5573907A (en) * | 1990-01-26 | 1996-11-12 | Abbott Laboratories | Detecting and amplifying target nucleic acids using exonucleolytic activity |
| WO1995021271A1 (en) * | 1994-02-07 | 1995-08-10 | Molecular Tool, Inc. | Ligase/polymerase-mediated genetic bit analysistm of single nucleotide polymorphisms and its use in genetic analysis |
| DE19520398B4 (de) | 1995-06-08 | 2009-04-16 | Roche Diagnostics Gmbh | Magnetisches Pigment |
| EP1154443A1 (en) | 2000-05-12 | 2001-11-14 | Boehringer Mannheim Gmbh | Magnetic glass particles, method for their preparation and uses thereof |
| WO1999003883A1 (en) * | 1997-07-18 | 1999-01-28 | Brigham And Women's Hospital | Compositions and methods based upon the tuberous sclerosis-1 (tsc1) gene and gene product |
| WO1999058664A1 (en) | 1998-05-14 | 1999-11-18 | Whitehead Institute For Biomedical Research | Solid phase technique for selectively isolating nucleic acids |
| AU3902799A (en) | 1998-05-14 | 1999-11-29 | University Of Hawaii | Compositions and methods for genetic transformation of pineapple |
| DE60015148T2 (de) * | 1999-08-20 | 2006-02-16 | Promega Corp., Madison | Simultane isolierung und quantifizierung von dna |
| US6548256B2 (en) | 2000-07-14 | 2003-04-15 | Eppendorf 5 Prime, Inc. | DNA isolation method and kit |
| WO2003057910A2 (en) * | 2002-01-08 | 2003-07-17 | Roche Diagnostics Gmbh | Use of silica material in an amplification reaction |
| GB0217963D0 (en) | 2002-08-02 | 2002-09-11 | Cyclops Genome Sciences Ltd | Purification of nucleic acids |
| EP1560926B2 (de) * | 2002-11-08 | 2013-08-21 | STRATEC Molecular GmbH | Neuartige pufferformulierungen zur isolierung, reinigung und rückgewinnung lang- und kurzkettiger nukleinsäuren |
| US20060240430A1 (en) * | 2002-12-04 | 2006-10-26 | Ying Wu | Method for hybridisation of immobilized genomic dna |
| CN1970752A (zh) * | 2006-11-29 | 2007-05-30 | 吉林大学 | 用磁性纳米复合材料回收dna的方法及试剂盒 |
| CN105441549A (zh) | 2007-03-30 | 2016-03-30 | 霍夫曼-拉罗奇有限公司 | 烷基胺改进甲醛固定的生物样品组分的检测 |
| CA2629589C (en) | 2007-04-20 | 2016-03-29 | F.Hoffmann-La Roche Ag | Isolation and purification of nucleic acid molecules with a solid phase |
| JP5292722B2 (ja) * | 2007-05-09 | 2013-09-18 | 凸版印刷株式会社 | 未精製血液をサンプルとする核酸増幅法 |
| EP2157181A1 (de) | 2008-08-13 | 2010-02-24 | AGOWA Gesellschaft für molekularbiologische Technologie mbH | Verfahren zur Isolation von Nukleinsäuren und Testkit |
| AT508355B1 (de) | 2009-06-29 | 2011-01-15 | Trumpf Maschinen Austria Gmbh | Verfahren und vorrichtung zum biegen eines werkstücks |
-
2010
- 2010-01-08 US US12/684,762 patent/US8420801B2/en active Active
-
2011
- 2011-01-05 CN CN201180005525.9A patent/CN102834518B/zh active Active
- 2011-01-05 EP EP11701607.1A patent/EP2521779B1/en active Active
- 2011-01-05 ES ES11701607T patent/ES2530466T3/es active Active
- 2011-01-05 WO PCT/EP2011/000013 patent/WO2011083076A1/en not_active Ceased
- 2011-01-05 JP JP2012547490A patent/JP5715158B2/ja active Active
- 2011-01-05 CA CA2786644A patent/CA2786644C/en active Active
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