JP5654226B2 - Plant metabolite collection device - Google Patents

Description

  The present invention relates to a method for collecting plant metabolites.

  There are methods to collect metabolites from plants. For example, there is a method of collecting loofah water by cutting a loofah stalk and collecting a tract fluid flowing out from the cut end.

  However, the loofah, whose stem has been cut, repairs the cut spontaneously. Therefore, in the above method, metabolites can be collected only during a temporary period until the cut end is repaired.

  It is an object of the present invention to provide a technique that enables collection of plant metabolites over a longer period of time than before.

A plant metabolite collection device according to the present invention for solving the above-mentioned problems is provided in a first collection tube connected to a xylem (path pipe, temporary path pipe) of a plant, and a sieve section (master tube) of the plant. A second sampling tube to be connected, and a permeable membrane is provided on a connection surface between the xylem and the sieve portion and the first sampling tube and the second sampling tube.

1 is a schematic configuration diagram of a plant metabolite collection system to which an embodiment of the present invention is applied, and an explanatory diagram for explaining a method of collecting a metabolite (nutrient) from a plant. It is a figure for demonstrating the effect by providing a permeable membrane in the connection part of a collection pipe. It is a figure which shows the outline | summary in the case of connecting a collection pipe to a fruit handle. It is a figure which shows the outline | summary in the case of connecting a collection tube to the cut surface of the stem of an ivy. It is a figure which shows the outline | summary in the case of connecting a collection pipe to a root. It is a figure which shows the outline | summary in the case of connecting a collection pipe to the branch of a branch (grafting method). It is a figure which shows the external appearance of the collection tube used for the connection by a grafting method. It is a figure which shows the outline | summary in the case of connecting a collection pipe to a part near the epidermis of a tree part. It is a figure which shows notionally the flow of a metabolite (tracheal fluid) at the time of connecting a collection pipe to a part near the epidermis of a tree part. It is a conceptual diagram of a metabolite collection controller. It is a conceptual diagram of a plant battery. It is a figure which shows the external appearance of the collection pipe | tube connected to a part of epidermis of a tree part.

  The present invention is an invention relating to a method for collecting a metabolite produced in a plant by connecting the collection tube to at least one of a xylem (path pipe, temporary path pipe) and a phloem (master tube) of the plant. . This will be specifically described below.

<Overview>
Some plants have a vascular bundle that penetrates the entire plant body. A vascular bundle consists of a xylem, a sieve part, etc. that play a role in transporting substances.

  There is a pipe (conduit) in the xylem. The canal is formed by connecting the canal elements in a tubular shape like “bamboo without knots”, and serves as a transport route for water and ions dissolved in water. Vascular elements are “dead cells” that have lost most of the plasma membrane and cytoplasm. The xylem soft tissue cells are in close contact with the vascular element, and exchange of ions dissolved in water with the vascular element is performed through the plasma membrane.

  Small vessel elements are "dead cells" that have lost their cell membrane, nucleus, and cytoplasm, but are originally cells that have differentiated from meristems.

  The mature vessel element has a thickened secondary cell wall and a large amount of lignin deposited (wood). Part of the cell wall between the vascular elements is lost, and the vascular elements are connected in a tubular shape like a bamboo with a knot.

  In addition, the temporary conduit (temporary conduit) works in the same way as the conduit element, but a thin cell wall that separates cells remains, and the aqueous solution moves through the cell wall from the temporary conduit to the temporary conduit. Most angiosperms have both canals and tracheids, but only feral canals, gymnosperms, and a few angiosperms connect only the tracheids that are connected to the aqueous solution.

  On the other hand, there is a phloem (sieve tube) in the sieving section. The mentor tube is formed by a series of mentor tube elements, and mainly serves as a transport route for anabolic products. The phloem element is formed by phloem cells and companion cells. The phloem cells communicate with each other through a “sheath plate” in which small pores due to large plasmodesum (protoplasm communication) exist, and transport water-soluble anabolic products. A phloem cell is a living cell, although it has lost much of its nucleus and cytoplasm, and relies on companion cells for substances necessary for the metabolism of the phloem cell itself.

  There are two types of plasmodesm: primary plasmodesm and secondary plasmodesm, which differ in the size (passage limit molecular weight), structure, and origin of substances that can be passed through.

  Primary plasmodesm is a structure that is actively formed during cell plate formation at the end of cell division, and the passage limit molecular weight varies depending on the tissue site.

  On the other hand, secondary plasmodesm is actively formed between cells after cell division. An example of the formation of secondary plasmodesm is “grafting” in plant individuals.

  Secondary plasmodesm is formed as a result of enzymatic degradation of existing cell walls. Plant individuals are thought to form secondary plasmodes enzymatically (ie, by gene expression) assuming their own “ideal” form. In plants, the presence of plasmodesm allows the entire plant individual to be regarded as “one polynuclear body”.

  By the way, when connecting a sampling tube to a tree part (path tube) or a sieve part (master tube) of a plant, the connection is made so that the plant recognizes the sampling tube as a part of the plant body (hereinafter referred to as “fusion connection”). ").

  As described above, the xylem (tracheal tube) is a “dead cell (a wooded state)” that has lost most of the plasma membrane and cytoplasm. Connection is possible as long as leakage and drying are dealt with.

  On the other hand, since the phloem part (the phloem tube) is a living cell in which the phloem cells are in contact with each other through the “tea plate” as described above, It is necessary to provide a permeable membrane or an absorption tube corresponding to the phloem plate between the sieve tube and the sampling tube. If the sampling tube is connected to the sieve tube without providing the permeable membrane or the absorption tube, it may cause a rejection reaction by the plant.

  By the way, if one uses a grafting technique in which cut surfaces of two plants (hogi and rootstock) are bonded to form one individual, one individual composed of genetically different parts can be made. For example, various types of plants such as pumpkin and cucumber grafts and watermelon and pumpkin grafts can be assimilated and bonded.

  Therefore, if such a grafting technique is used, the collection tube can be fused and connected to the plant. For example, by mixing plant hormones (proteins, peptides, etc.) possessed by plants into the connecting surface between the collection tube and the plant (at least at the tip of the collection tube), the union (anabolism) of the collection tube and the plant is promoted. be able to. Alternatively, plant hormones may be applied around the collection tube or as an adhesive to promote fusion (anabolism).

  In animal bodies, highly independent animal cells adhere to each other to form tissues and individuals. In cell adhesion of animal cells, structures for cell adhesion such as desmosome and hemidesmosome are mediated.

  In contrast, in plants, all cells of an individual are originally connected by a continuous cell wall, and there is no structure for cell adhesion. In addition, as described above, in plants, protoplasms of cells are partially shared by a structure called protoplasm communication (plus modems) that penetrates the cell membrane. From this point, it can be said that the whole plant has the property as one giant polynuclear body.

  However, when plant free single cells form agglomerates, the cells should be bound together in some way, such as cell adhesion in animal cells.

  For example, plasmodesm is thought to provide a bridging structure between adjacent cells and fill a gap (to connect cells together) so that both cells are assimilated by cell division.

  The secondary plasmodesm that exists between the vascular elements that form the vascular system is a structure that is generated by enzymatic degradation of existing cell walls. However, as described above, the vascular element should be a “dead cell” that has lost most of the plasma membrane, nucleus, and cytoplasm, and cannot biosynthesize cellulase or pectinase by itself. Therefore, it is thought that the enzyme group which decomposed | disassembled a part of cell wall of the vascular element and formed secondary plasmodesm originated from another cell.

  From these facts, it can be said that the role of enzymes in plants is important. For example, enzyme control leads to cell adhesion and promotion of plant growth. In addition, adjusting the health condition of the target plant from which the metabolite is collected, the production amount of the metabolite (including secondary metabolites), the components of the metabolite, etc. also leads to cell adhesion and promotion of plant growth.

  By the way, plants receive sunlight and perform photosynthesis by photochemical reaction. The sugar produced by photosynthesis can be said to be a form in which solar energy is converted to chemical energy. Sugar is also a raw material for the synthesis of high-molecular polysaccharides, lipids and other substances. At the same time, it is oxidized and its energy is converted into a form of adenosine triphosphate (ATP) that can be used by living organisms. When such a process (chemical reaction) is efficiently performed using molecular oxygen, it is called “breathing”. The process of synthesis and decomposition (chemical reaction) of such organic compounds is called “metabolism” and is catalyzed by oxygen.

  Glucose produced by photosynthesis is partially broken down by cells and becomes water and carbon dioxide again. The energy of ATP obtained at this time is used for synthesis of storage substances such as protoplasmic components, cell wall components, and lipids using glucose as a raw material. The energy generated by respiration is used not only for such synthetic work but also for osmotic work (such as respiration of substances). In this way, it is possible to retain and express genetic information unique to an organism.

  The ATP described above is a substance that plays an important role in energy conversion in metabolism in plant cells or cells of living organisms in general. ATP is one of high-energy phosphate compounds that release a large amount of energy when hydrolyzed. ATP is involved in various biochemical reactions in the cell and hydrolyzes, so it is not released as energy heat, but is used to perform those biochemical reactions. Therefore, it can be said that ATP is chemical energy that can be used in biological reactions.

  In the process of breathing, “glucose → pyruvic acid → water + carbon dioxide”. In the process of “glucose → pyruvic acid”, oxygen is not required, and this chemical reaction is called “anaergic breathing”, “anaerobic breathing”, or “glycolysis”. In the process of “pyruvic acid → water + oxygen dioxide”, molecular oxygen is required, and this chemical reaction is called “aerobic respiration”. This chemical reaction is divided into three stages: <1> reaction of citric acid circuit (TCA circuit), <2> electron transfer, and <3> ATP generation. By the way, there is also a reaction that decomposes glucose without consuming oxygen in plant cells, such as in the early stage of germination (the same reaction as glycolysis), and is particularly called “fermentation”. In addition to lactic acid, ethanol is also produced during fermentation.

  The energy conversion efficiency in respiration as described above is {(36 molecules × 7.3 kcal) / 686 kcal} × 100 = 38%, which is much more efficient than the energy conversion efficiency using a heat engine.

  By the way, plants do not accumulate alcohol or lactic acid in the air. However, since pyruvic acid is synthesized in both anaerobic and aerobic breathing, the initial stages of anaerobic breathing and aerobic breathing are the same. That is, like aerobic respiration, the product of anaerobic respiration is carbon dioxide in plants. Phosphorylation occurs in both anaerobic breathing and aerobic breathing.

  In addition, hydrogen liberated by the reaction in the citric acid cycle is ionized, and electrons are transferred to oxygen via the cytochrome system. In such a redox reaction, electrons flow from a substance having a low electron pressure (that is, a negative substance) to a substance having a high electron pressure (that is, a positive substance). Here, the substance having a low electron pressure is called an electron donor, which is a so-called reducing agent. A substance having a high electron pressure is called an electron acceptor, which is an oxidant. An electron donor has an inherent electron pressure, and an electron acceptor has an inherent electron affinity. These electron pressure and electron affinity can be measured by electromotive force (that is, potential). In addition, the potential when each substance is measured in the standard state is called a standard reduction unit.

  Also, eukaryotic cells such as plants that perform aerobic respiration have mitochondria. The mitochondria undergo a reaction between the citric acid cycle and the respiratory chain, and oxidative phosphorylation. Mitochondria has a shape close to an ellipse having a length of 0.4 to 5 μm and a thickness of about 0.3 to 0.8 μm. There are several to thousands of mitochondria in one cell. Mitochondria contain enzymes in the citrate cycle and electron transport system, and also have prokaryotic DNA and ribosomes, and are considered to have self-propagating properties.

  The respiration rate in plant cells depends on protoplasmic state, respiratory oxygen activity, respiratory substrate supply, etc., but these internal factors are significantly influenced by external factors. External factors include temperature (maximum at 30 to 40 ° C.), oxygen partial pressure (0.2 atm, but 20 to 25% oxygen concentration), and the like.

  By the way, the ratio of the volume of carbon dioxide released by respiration in plants and the volume of absorbed oxygen (that is, “volume of carbon dioxide / volume of oxygen”) is called a respiratory quotient (RQ value). When seeds germinate, the respiratory quotient fluctuates significantly because the respiratory substrate varies depending on the seed type and the time after germination.

  In addition, glucose, which is a photosynthetic product, transforms into a sucrose form. An important storage polysaccharide of plants using glucose as a raw material is starch, and a structural polysaccharide is a cell wall polysaccharide. This is converted by sugar phosphorylation.

  One important metabolic pathway in sugar conversion is the pentose phosphate pathway (PPP), which in many ways resembles the dark reaction of photosynthesis. The pentose phosphate pathway occurs outside the chloroplast, ie in the cytoplasm, and the enzyme is also present in a soluble form in the cytoplasm.

  By the way, in said photochemical reaction, ATP production | generation is performed by the excitation of the electron by light energy, and the electron transmission accompanying it. Only the plants and some bacteria (photosynthetic bacteria: cyanobacteria, red sulfur bacteria, etc.) have a photosynthetic reaction. In the case of oxygen-generating photosynthesis, water is used as the electron donor. In the case of non-oxygen-generating photosynthesis, several organic compounds (such as propanol) including hydrogen sulfide and hydrogen are used as electron donors.

  The Calvin circuit (Calvin-Benson circuit) is a typical carbonic acid fixation reaction in a photosynthesis reaction. Almost all green plants and photosynthetic bacteria possess this circuit.

  NADPH and ATP generated by the photochemical reaction are driven to rotate the circuit, and finally enter the gluconeogenic pathway from fructose-6-phosphate to become a polysaccharide (starch). Ribrose bisphosphate carboxylase (RubisCO), which is responsible for the carbon fixation reaction, which is the core of this circuit, is said to be the most abundant enzyme present on the earth.

  Further, since the reaction itself proceeds even without light, it is also called a dark reaction in contrast to a photochemical reaction (light reaction) in which light is indispensable. However, among the enzymes involved in the reaction, a plurality of enzymes such as RuBP carboxylase and phosphatase are indirectly activated by light, and thus lose their activity within a few minutes when dark.

  It is a bright reaction that electron transfer takes place. In the light reaction of photosynthesis, when electrons excited by light are transmitted through the photochemical system, hydrogen ions are transported to the thylakoid lumen and NADP + is reduced to produce NADPH. Electrons are extracted from water using the energy of light, and as a result, oxygen is generated.

  On the other hand, in the inner mitochondrial membrane, electron transfer occurs following the oxidation of NADH. Using the energy from electron transfer (continuous redox reaction), hydrogen ions are transported from the mitochondrial substrate to the outside of the inner membrane. As a result, there is a hydrogen ion concentration gradient inside and outside the membrane in the mitochondria. Since hydrogen ions have a charge, a potential difference also occurs at the same time. Since electron transfer occurs, this series of reactions (or enzyme groups responsible for the reaction) is called an “electron transfer system”. The ultimate recipient of electrons is oxygen, which results in water.

  The above-mentioned ATP is obtained by attaching two phosphates to adenosine, which is a component of DNA, and it can be said that ADP and phosphate are bound by the action of an enzyme.

  Molecules that are digested and absorbed in the body after ingesting carbohydrates, proteins, and fats are processed through three stages (glycolysis, citric acid cycle, and electron transfer) to produce ATP as an energy source. Of the three stages, glycolysis is carried out in the liquid part of the cytoplasm (cytoplasmic matrix, matrix), and the citrate cycle is carried out in the intracellular mitochondrial matrix. Electron transfer occurs in the inner mitochondrial membrane.

  There are several protein complexes among the precursor electron transport proteins that produce ATP. The largest source of ATP, which is an energy source, is glucose (glucose) from which carbohydrates are decomposed. Glucose is broken down into pyruvate and enters the citric acid cycle via acetyl CoA. Pyruvate produces bimolecular ATP without oxygen. By the reaction of the tricarboxylic acid cycle linked to the respiratory chain, pyruvic acid is completely oxidized to form carbon dioxide and water, and promotes strong ATP formation.

  Nutrients become more energy-efficient as they go through stages, but without a bridging substance, their resources may not go through all stages. Electron transfer is important for ATP production, and there is ubiquinone (coenzyme Q10), which is said to be an electronic messenger active in electron transfer.

  The main component of the cell wall is cellulose, which accounts for 25 to 35% of the cell wall in young cells and about 50% in wood. Cellulose is a polymer in which glucose produced by photosynthesis is linked in a chain. In addition to cellulose, the cell wall contains hemicellulose and pectin that are easily decomposed. New cells are enveloped by primary walls made of cellulose and hemicellulose fibers. In order to decompose and digest cellulose, an enzyme called cellulase is required. Herbivores degrade cellulose, but instead of synthesizing cellulase by themselves, microorganisms in the intestine have cellulase, which is degraded. Plants themselves have cellulases, which degrade cellulose when necessary. For example, it is used to make a delamination at the base for fallen leaves, so it is thought that the generation will increase at this time.

  When replicating DNA, unwind the double helix and use each sequence as a template to make another base sequence. A marginal telomere sequence is repeated at the end of the DNA to allow room for primer attachment at the end.

  Telomeres become shorter with division. However, the creature will eventually become extinct. Therefore, there is an enzyme called telomerase in living organisms to regenerate telomeres. There are also telomeres at the ends of plant chromosomes. Telomerase has also been found in dividing cells other than pollen-producing tissues, but it is thought that telomerase production increases during the flowering period when pollen is applied.

  On the other hand, for example, when one plant grows in the shade of another plant and cannot escape from the shade, the plant will grow up early, make it bloom, make seeds, and make the next generation possible. Take the strategy of connecting. This can be prematurely bloomed by removing the “spider” that has suppressed the FT gene from working.

  Based on these facts, the present invention collects polysaccharides (starch) and glucose (glucose) produced in plant photosynthesis from a collection tube. Then, the role of mitochondria performed in the respiratory system is performed in the collection controller device. The harvesting controller device also functions as a plant sink organ by returning the product produced in the device to the plant.

  In addition, the present invention can collect cellulase, telomerase, and plant hormones that degrade cellulose, collect enzymes such as gibberellin and cytokinin, and return them to plants. Moreover, electric power is obtained by combining a part of the citric acid circuit or a part of the citric acid circuit as a plant battery based on the extracted glucose. Furthermore, alcohol fermentation and lactic acid fermentation are caused to obtain alcohol and lactic acid.

<Example of Embodiment>
Hereinafter, an example of an embodiment of the present invention will be described with reference to the drawings.

  In the animal world, dairy cows (Holstein) produce milk after parturition. Thereafter, the dairy cow continues to produce milk by continuing to squeeze the milk. This is due to the fact that humans continue to squeeze the milk that is given to the child, so that the body of the cow is regarded as being needed by the child and continues to deliver milk.

  Actually, after one delivery, the period during which milk can be continued is limited to about 10 months, but is repeated again at the next delivery. On the other hand, calves are weaned in about two months.

  In the plant world, there are many plants that attach fruits to plants. Fruit grows on the basis of nutrients made by photosynthesis of plant roots and leaves.

  When the fruit grows, remove the pre-ripe fruit from the stalk, continue to collect the nutrients (metabolites) that are originally given to the fruit, and the plant body behaves as if the fruit requires it. Will continue to send. However, there are limits to the period and amount, and it is necessary to select plant types and varieties in consideration of economic effects and target more effective plants.

  Moreover, in plants that sprout in the spring, when the shoots extend, nutrients (metabolites) continue to be sent from the leaves and roots to the shoots. As long as the shoots continue to grow, this nutrient will continue to be sent.

  FIG. 1 is a schematic configuration diagram of a plant metabolite collection system 10 to which an embodiment of the present invention is applied, and an explanatory diagram for explaining a method of collecting a metabolite (nutrient) from a plant.

  First, as shown in FIG. 1 (a) to FIG. 1 (b), the fruit (for example, citrus fruit) 11 is removed so as not to damage the fruit pattern 12 of the fruit 11 before ripening. Next, as shown in FIG. 1 (c), the sampling tube 100 is attached to the tip of the fruit handle 12. At this time, when the collection tube 100 is securely attached to the fruit handle 12, the connection portion can be prevented from drying. Further, in order to promote adherent cells, a member (for example, growth hormone or enzyme) containing or synthesizing a protein may be used (for example, applied) to the connection portion. In order to securely attach the fruit handle 12 and the sampling tube 100, a flexible protective tape or the like may be wound around the outside of the connection portion.

  A collection tank 110 is provided at the end of the collection tube 100 (the end opposite to the tip). The collection tank 110 stores a collected solution collected from plants. In addition, the collection tank 110 is provided with a faucet 115 for extracting the stored collection liquid. In addition, in order to separate and store the collected liquid collected from each of the canal and phloem, the collection tank 110a for the tract that stores the collected liquid collected from the canal and the collected liquid collected from the pedestal are stored. And a collection tank 110b for the teacher tube may be provided separately. However, the present invention is not limited to this, and a metabolite (nutrient) may be collected from at least one of the tract and the phloem.

  At the tip of the collection tube 100, a permeable membrane (including a semipermeable membrane and a reverse permeable membrane) 120 corresponding to the mentor plate 22 and the mentor zone 23 in the mentor tube of the mentor cell 20 is provided. The permeation membrane 120 is provided with a permeation hole, and the gap (behind the collection tank 110 side) is filled with a sea surface-like sponge body 170 or the like. Thereby, the connection which does not impair the penetration effect becomes possible.

  FIG. 2 is a diagram for explaining the effect of providing the permeable membrane 120 at the connection portion of the sampling tube 100. In the example shown in FIG. 2 (a), upper and lower phloem cells 20 and companion cells 21, a mentor plate 22, and a mentor area 23 are shown. Further, as shown in FIG. 2B, when the mental tube is cut, the mental plate 22 does not exist on the cut surface of the mental tube or the companion cell 21. Therefore, if the collection tube (capillary tube) 100 is directly joined to the cut surface of the phloem tube or the companion cell 21, there is a possibility that metabolite penetration cannot be performed well. Therefore, in the present invention, as shown in FIG. 2 (c), an osmotic membrane 120 is inserted between the cut surface of the phloem tube or companion cell 21 and the collection tube (capillary tube) 100, and the original phloem tube penetrates. The effect is not impaired.

Returning to FIG. As shown in the figure, a structure in which a plurality of thin tubes are combined or a structure in which a plurality of fibers are bundled is provided inside the collection tube 100 in order to introduce a metabolite to the storage tank 110. Thereby, penetration of a metabolite or a liquid and capillary action can be promoted. In addition, the collection tube 100 may be provided with a motorized valve (which may be an electromagnetic valve or a check valve) 190 for adjusting the amount of collected metabolite. In addition, the collection tube 100 may be provided with a suction / reverse motor for returning metabolites to plants (eg, a tract tube, a phloem tube) according to time, time zone, climate, and the like.

  In addition to root pressure, plants carry water by a cohesive force in which water molecules from each other stick together. By utilizing the cohesive force due to the transpiration, a transpiration function may be provided in one of the collection tubes 100 to collect a metabolite from a branch point in the middle.

  Although not shown, the collection tube 100 is fixed with a band, tape, or the like in order to prevent it from coming off from a plant (such as a road tube or a teacher tube) due to wind or rain.

  FIG. 3 is a diagram showing an outline when the sampling tube 100 is connected to the fruit handle 12 of a large tree (eg, palm, papaya). As shown in FIG. 3A to FIG. 3C, the fruit 11 is removed so that the fruit pattern 12 of the fruit 11 is not damaged, and the sampling tube 100 is connected to the fruit pattern 12. Further, as shown in FIG. 3D, the collection tube 100 includes a tube (hereinafter referred to as “absorption tube 101”) for extracting metabolites and the like from a plant (source organ), and a collection tank (sink). (Organ) 110 and a tube for returning metabolites and the like to the plant (hereinafter referred to as “inlet tube 102”). Of course, the same collection tube (collection tube 100a for the tractor tube, collection tube 100b for the mentor tube) 100 is connected to each of the tractor tube and the phloem tube, and a total of four tubes are connected to the plant 1 (fruit handle 12). You may do it.

  FIG. 4 is a diagram showing an outline when the sampling tube 100 is connected to the cut surface of the stem 30 of the ivy such as pumpkin, loofah, and kuzu. As shown in FIGS. 4A to 4B, the stem 30 of the plant 1 is cut, and the sampling tube 100 is connected to the cut surface. As shown in FIG.4 (c), the inside of the collection pipe | tube 100 becomes a structure where the pipe | tube was doubled. In other words, a phloem-collecting tube 100b is inserted into a tractor-collecting tube 100a. Of course, not only when the collection tube 100 is connected to the stem 30 of the ivy, but also when the collection tube 100 is connected to the fruit handle 12 or the like, the collection tube 100 having the same structure may be used.

  FIG. 5 is a diagram showing an outline when the sampling tube 100 is connected to the root (for example, kudzu, sweet potato, lotus root, potato) 31. As shown in FIG. 5A to FIG. 5C, the rhizome and root 31 are peeled off so as to leave the core portion 32, and the sampling tube 100 is connected. Further, the connecting portion is buried in the ground so that the sampling tube 100 does not move due to wind or rain. Further, in order to prevent the collection tube 100 from being detached even if the rhizome or the root 31 grows (extends) in the ground, a space 33 is provided in the ground so that the root 31 and the collection tube 100 can move together. Good.

  FIG. 6 is a diagram showing an outline of a case where the sampling tube 100 is connected to the branching branch 34 (grafting method). As shown in FIGS. 6 (a) and 6 (b), the collection tube 100 is divided and connected to a branch or leaf branch 34 so as to graft a young shoot. Moreover, as shown in FIG.6 (c), the connection part of the front-end | tip 104 of the collection tube 100 is made into a wedge shape, and in order to cope with the enlargement by growth (in the figure, the grown plant 1a is shown), it is flexible. (104a). Further, the narrow tube 103 may also be formed of an extending material, and the interval between the thin tubes 103 may be widened. Note that the connecting portion is preferably fixed with a tape or the like.

  FIG. 7 is a diagram showing an appearance of the sampling tube 100 used for connection by the grafting method. As shown in the drawing, the distal end (tip end) 104 of the collection tube 100 is formed by collecting the narrow tubes 103 with a sleeve portion 211. A protective sleeve 212 is provided in order to bring the graft and the connecting portion into close contact with each other. Some of the thin tubes 103 are led to the absorption tube 101 and supply metabolites to the collection tank 110. On the other hand, the remaining thin tubes 103 are guided to the inflow tube 102 and return the metabolites from the collection tank 110 to the plant. Each thin tube 103 may be a hollow tube, may be a bundle of fibers (a metabolite can be passed between fibers), or may be an osmotic (absorbable) fiber or non-woven fabric. Further, the vicinity of the tip of each thin tube 103 may be covered with a permeable membrane (including a semi-permeable membrane and a reverse permeable membrane) 120. In the sleeve portion 211, a permeable membrane (permeable membrane 120) may be enclosed in a connection portion between the thin tube 103 and the absorption tube 101 or a connection portion between the thin tube 103 and the inlet tube 102.

  FIG. 8 is a diagram showing an outline in the case where the sampling tube 100 is connected to a part near the epidermis of the tree part. As shown in FIG. 8A to FIG. 8B, the collection tube 100 includes a tube joint portion 130 and a branch portion 140. A cutout portion 150 corresponding to the pipe joint portion 130 of the sampling tube 100 is cut out in the epidermis of the trunk 2 of the tree. Then, the pipe joint part 130 of the sampling pipe 100 is fitted into the cut-out part 150 and is fastened with the adhesive tape 500. As a result, the metabolite can travel back and forth through the pipe joint portion 130 through a portion of the canal or phloem near the epidermis of the tree.

  After the transplantation of the pipe joint portion 130 has been smoothly recovered (after the pipe joint portion 130 has functioned as a passage or a master tube), a metabolite is collected from the branch portion 140.

  In the pipe joint portion 130, a bundle of thin tubes 103 are closely connected in the vertical direction, and each thin tube 103 is connected to an outer tube (insertion tube 160) by a branching portion 140. Here, each narrow tube 103 is branched (classified) into two systems. For example, each narrow tube 103 may be branched (classified) into two systems such as a road tube and a mentor tube, or may be branched (classified) into two systems such as an absorption tube 101 and an inlet tube 102. Moreover, each thin tube 103 may be collected by one system. In this case, the same sampling tube 100 is fitted into the hollowed portion 150 at a plurality of locations in the tree part, and one sampling tube 100 is used as a collection tube 100a for a road tube, and the other sampling tube 100 is used as a collection tube for a master tube. 100b.

  FIG. 9 is a diagram conceptually showing the flow of a metabolite (tracheal fluid) when the collection tube 100 is connected to a part near the trunk 2 epidermis of the tree part. In the figure, the pipe joint portion 130 is separated from the cutout portion 150 on the surface of the tree. As shown in the drawing, a thin tube 103 is arranged in the longitudinal direction at the distal end portion (pipe joint portion 130) of the collection tube 100, and a cavernous body 170 is installed on the insertion tube 160 side. Metabolites that travel back and forth through the thin tube 103 reach the insertion tube (outer tube) 160 through the cavernous body 170.

  The thin tube 103 is a pipe-like tube in which a small hole having permeability is provided on the outer wall. The sap (liquid containing metabolites) of the canal of the tree flows in the direction 206 (up and down) of the pipe joint portion 130 through the flow 205 between the canal and the thin tube 103, and the companion cells of the tree and the thin tube 103. Sap also flows in the direction of the flow 204 between them and in the direction of the flow 207 of the narrow tube 103 and the cavernous body 170.

  The tip of the sampling tube 100 is covered with a cover portion 180, and prevents the joint from being dried and sap leakage when connected to a tree.

  Although not shown, after the collection tube 100 is embedded in the tree (after connection), the surface of the cover unit 180 and the tree may be attached (adhered) with a tape or the like.

  The thin tube 103 may be replaced with a material that is permeable in the up-down direction and the left-right direction, such as a bundle of fiber-like tubes, a nonwoven fabric, or a sponge.

  In the present embodiment, the skin portion of the tree is the connection target of the sampling tube 100, but a notch is provided in a wedge shape toward the core of the tree, and the sampling tube 100 corresponding to the shape of the notch is the notch. You may connect so that it may fit.

  For example, FIG. 12 shows a collection tube 100 constituted by a porous collection tube distal end portion 104 b in which the distal end (tip portion) 104 of the collection tube 100 is a porous portion 106. A porous portion 106 made of a porous material is provided on the inner side of the cover portion 180, and an inlet liquid pore network 107 is provided as a groove for allowing metabolites to pass between the inside of the porous portion 106 and the inlet pipe 102. Provided. The porous material may be a hard material such as ceramics or metal, or may be a sea surface body or a sponge-like soft material, as long as the metabolic fluid flows between the inlet tube and the plant. The inflowing liquid hole network 107 increases the flow thereof, and is like a water channel in which a groove is stretched around the porous portion 106.

  The coconut tree is a monocotyledon like bamboo, and the vascular bundle with the phloem part set together with the xylem is scattered in the stem (asymmetric central pillar), so it connects to correspond to the scattered vascular bundle. Is good.

  FIG. 10 illustrates a metabolite collection controller 300 that collects metabolites from a plant via the collection tube 100 and stores the metabolites in the collection tank 110, a plant battery 400 that accumulates charges using the metabolites collected from the plants, The conceptual diagram of is shown.

  As shown in FIGS. 10A to 10C, the metabolite collection controller 300 collects plant metabolites (mainly glucose) from the absorption tube 101 side of the collection tube 100. The metabolite collection controller 300 includes a first suction pump 310 for adjusting the amount of collected metabolite. Then, the metabolite collection controller 300 uses the first suction pump 310 to set the time (for example, from June to August), the time (for example, morning), the environmental temperature, the humidity, and the amount of sunlight (for example, a sunny day). ) Adjust the amount of metabolite collected according to Then, the metabolite controller 300 uses a dialysis machine such as diffusion, ultrafiltration, and osmotic pressure to extract the extracted sugar from the dialysis machine. In the illustrated example, extraction at the semi-permeable membrane 320 is shown. A liquid (metabolite) containing a metabolite (such as sugar) extracted by the dialysis machine is stored in the collection / concentration tank 110. Then, the metabolite stored in the collection / concentration tank 110 is sent to the plant battery 400 using the first delivery pump 360. In the collection / concentration tank 100, the fuel of the plant battery 400 having a high power storage effect can be made by increasing the concentration of sugar (glucose) or separating other components.

  FIG. 11 shows a conceptual diagram of a plant battery 400 that accumulates charges using metabolites collected from plants.

  The plant battery 400 converts (enzymatically decomposes) glucose (also known as glucose) sent from the collection / concentration tank 110 into gluconolactone (also known as humic acid) using an enzyme group. As a result, electrons (e−) are obtained from an electron transfer material (such as a porous carbon rod) to form a negative electrode. In addition, ATP is produced by the enzymatic decomposition here.

  On the other hand, the plant battery 400 combines the hydrogen ions that have passed through the separator 410 with the oxygen delivered from the delivery pipe 401 (a delivery pipe for supplying oxygen) via the opposing enzyme group, thereby generating water. At the same time, a positive electrode is formed via an electron transfer material (such as a porous carbon rod).

  In this way, the plant battery 400 can take out the potential difference generated between the positive electrode and the negative electrode as electric energy (for example, use it as electricity, charge the battery, etc.).

  In addition, the gluconolactone produced in the plant battery 400 is provided in the metabolite collection controller 300 through the delivery pipe 404 (gluconolactone delivery pipe) and the water through the delivery pipe 402 (water delivery pipe). The suction pump 330 and the third suction pump 340 are sucked up and stored in the water supply liquid tank 350 as the water supply liquid 210 by the required amount. Alternatively, it is stored separately in a solution and reused. In the water supply liquid tank 350, the collected liquid 200 dialyzed by a dialysis machine is also stored. Alternatively, they are mixed and stored at a ratio as required.

  The water feed liquid in the water feed liquid tank 350 is returned to the plant by the second feed pump 370 through the feed pipe 102 of the collection pipe 100. In addition, the water supply liquid returned to the plant includes components, concentrations (for example, metabolite concentration, enzyme concentration) and amount (for example, metabolite amount, enzyme amount), plant body fluid ( In consideration of the fluid pressure of the sap), it is sent to the plant. Further, the water supply liquid of the water supply liquid tank 350 contains minerals, proteins, and other nutrients. The water supply liquid in the water supply liquid tank 350 may be used not only for feeding into plants but also for other purposes. Although not shown, plant hormones or minerals collected or synthesized elsewhere may be mixed.

  Adjustment of the amount collected from the plant via the collection tube 100, adjustment of the amount delivered to the plant via the collection tube 100, activation of the plant battery 400, adjustment of the amount of suction from the plant battery 400, etc. Controlled by connected computer system. Here, the computer system includes a CPU, a memory, an auxiliary storage device, an input device, an output device, a communication device, various sensors, and the like. The various adjustments described above are established by the CPU reading and executing a predetermined program. Therefore, the storage device (memory) stores programs and data tables for realizing the various adjustments described above. As a result, collection suitable for plants is possible (metabolites can be collected from plants efficiently).

  Here, the plant battery 400 using oxygen is shown, but there are also a plant battery using anaerobic breathing and a method of changing not only to carbohydrates but also to energy such as amino acids and fatty acids.

  Usually, when the collection tube 100 is attached to a plant, the sap comes out from the cut portion of the plant. At this time, the inside of the collection tube 100 is filled with the sap by joining the collection tube 100 to the plant while the sap is not solidified. When the collection tube 100 is filled with sap, the exchange of metabolites between the plant and the collection tube 100 can be performed smoothly by capillary action and the cohesive force of water. In order to utilize the cohesive force of water, air (including water vapor and the like) is prevented from entering the narrow tube 103, and as a countermeasure when it enters, it is useful to control to suck and push with a pump at the time of activation.

  Hereinafter, this invention is demonstrated using the Example according to the kind of plant. However, the present invention is not limited to these examples.

<Example 1: Method for collecting loofah water>
Conventionally, there is a method of collecting loofah water as a method of collecting vascular fluid. Loofah water (fluid fluid) is a bodily fluid of plants. The fluid flowing in this canal contains hormones, proteins, carbohydrates, etc., in addition to nutrient salts. These organic substances are produced by the roots and secreted into the canal, and are essential for the life of plants, especially above-ground organs. Loofah water and pumpkin water can be taken from one bottle to about 1 liter. The collection method can be obtained by cutting the stalk, stab the cut stalk into a bottle, etc., and covering it so as not to dry. Since it will go out in about a day, it is necessary to cut another stem and pierce the bottle.

  Thus, the current method for collecting loofah water can be said to be a method of cutting the loofah stem and taking out the vascular fluid flowing out from the cut end. In addition, this collection method is effective only during the period until the plant body is damaged and the flow of the vascular fluid is stopped by repairing the cut end by the plant. Therefore, it can be said that it is a temporary collection method.

  Vascular fluid is the fluid that flows through the plant's canal, mainly from the roots to the above-ground organs. The vascular duct, which is one of the constituent elements of the vascular bundle, is a tube in which the shells (cell walls) of tubular element cells in which programmed cells die are connected, and is an extracellular space surrounded by parenchyma cells in the vascular bundle. In addition to water and minerals (inorganic ions) absorbed from the soil in the roots, the vascular fluid is composed of amino acids (such as glutamine) synthesized by the root cells (such as glutamine), plant hormones (such as zeatin riboside and abscisic acid), proteins ( It contains organic substances such as lectins, arabinogalactan proteins, etc.) and carbohydrates (fructose, glucose, etc.). It is mainly transported by transpiration during the day and by root pressure at night (pressure of water going into the vascular bundle to fill the difference in mineral concentration inside and outside the vascular bundle).

  The phloem liquid is a liquid that flows through the phloem of plants mainly from leaves (source organs) to buds, roots, fruits, etc. (sink organs). One of the components of the vascular bundle, the mentor tube is a tube composed of continuous protoplasms in which mentor element cells that have lost their nuclei are connected through the mental plate. The components of the phloem fluid consist of protoplasms containing proteins, high concentrations of sucrose, amino acids (glutamine), etc., which are supplied from companion cells through protoplasm communication. The anabolic product (sucrose) is transported mainly from the source organ (adult leaf) to the sink organ (root, fruit, bud) due to the difference in the concentration in the phloem tract (sink, source).

  As described above, the vascular fluid moves from the root toward the stem mainly due to a difference in mineral concentration (difference in osmotic pressure). Therefore, in this embodiment, the vascular fluid is collected by controlling these components through the collection tube 100.

  In addition, the phloem fluid moves due to a difference in concentration at a site (sink organ and source organ) in the phloem. For this reason, in this embodiment, the phloem fluid is collected mainly by providing the collection tube 100 in the sink organ (bud, root, fruit, etc.).

<Example 2: Method of collecting metabolite from kuzu>
Kudzu belongs to the Azuki tribe genus of the Leguminosae family in the plant classification. From the beginning of sprouting from perennial stems and strains that have overwintered until before the rainy season, stem growth is relatively slow. However, after a while after entering the rainy season, the rate of elongation increases rapidly, reaching a peak from late July to early August. After that, during the intense heat from mid to late August, the growth is temporarily suspended.

  This is because the overgrowth of the community reached the extreme, resulting in many dead leaves due to lack of light or poor ventilation in the community, and the growth of the new year's stem temporarily slowed down. This is thought to be due to the fact that there is a slight increase in

  Katsu grows again from early September and stops around early October. Meanwhile, it blooms from late August to mid-September. Thereafter, the leaves begin to turn yellow from around early November, and the stem begins to wither from the tip. In the meantime, when a strong frost is encountered, the leaves appear to be completely withered and then turn dark brown and die. In parallel with the yellowing of the leaves, the stem is hardened with a brownish surface from the strain and begins to become woody.

  Perennial plants, similar to kuzu, store substances based on carbohydrates in their storage organs. Kuzu not only stores a large amount of carbohydrates in the main root, but also has a small amount of storage material in perennial perennial stems. These storage materials are sent to the growth part early in the spring to support the rapid growth of new organs.

  The new organ is made by the photosynthesis of the leaf and the substance sent from the storage period, but if this substance is a carbohydrate, a new plant with about half the amount of substance decrease in the storage period by dry weight will be created. I know. As a result of experiments based on this fact, it has been found that about 67% of the current year's stems and leaves made by the end of July were made by stored substances. This can be said to be the source of great advantage for the growth of the fierce conflict. The perennial stems and roots that have been exhausted are fed anabolic substances from the perennial organs after August, which rapidly increases the specific gravity of the storage organs. In this way, the circulation is repeated in which the material stored from summer to autumn is used again to form the next growing organ.

There are two kinds of perennial stems and perennial stems. As for the kuzu leaf area, the leaf area of one individual in a prosperous area is about 5-8 m ² in October.

  The root system of the kuzu has a main root rooted deep in the ground from the stock and a root root rooted from the stem.

  Since the main root penetrates deeply in the ground, its shape is an enlarged spindle shape in the middle, also called a tuberous root. This spindle enters one or two strands from the strain, and further roots by branching off a few thinner spindles from the spindle. These main roots store a large amount of nutrients, and since long ago they were dug in the fall to produce starch (so-called storage roots).

  The knot root is not as big as the main root, but the kudzu is a plant with many leaves and intense transpiration, so it is thought that it plays an important role in the growth of the kuzu together with the main root to replenish water.

  From this, it can be said that the sink organ does not require nutrients during the period when the elongation is suspended for a period of time in the mid-August to late summer. Conversely, this period can be a period during which more nutrients can be collected from the phloem collection tube connected to the sink organ.

  In addition, since anabolic substances are sent from this year's living organs after August, it becomes a period in which more collection is possible depending on the collection tube provided at the main root.

  Kuzu starch has been produced from katsu for a long time. In addition, starch is taken from starches such as oats, bracken, katakuri, lily, and ganga in Japan.

<Example 3: Collection method of maple syrup>
Maple syrup is collected during the 6-8 weeks from the end of winter to the beginning of spring, when sugar maple grows in the extremely cold region, and the sugar content of the sap is increased and sweetened to survive the cold in winter. When the daytime temperature exceeds 0 degrees, the sap flow is created in the tree due to the change in temperature, so it is collected at that time. The sap flow stops within the day, but this time it absorbs moisture from the roots at night. If the temperature rises the next day, sap will come out again. In this way, the sap is used to produce the sugar that is aged during the winter.

  Maple accumulates starch during the growing season of the tree. When the snow melts, the starch turns into sugar due to the action of enzymes and mixes with the water sucked up from the roots, resulting in a slightly sweet sap. To purify 1 L (liter) of syrup, 40 L of sap is required.

  Maple syrup contains minerals such as calcium, potassium, magnesium, phosphorus, manganese, and iron, and vitamins B2, B5, B6, C, PP, etc., and is also a health food. In particular, calcium is 15 times more abundant than honey.

  The sap is taken by drilling a hole with a diameter of about 1 cm and a depth of about 5 cm, and receiving the sap with a bucket. In order not to damage the trees, there are no more than three holes and any sugar maple. Trees from which sap is collected are over 100 years old, have a diameter of 25 cm or more, and the amount of collection is limited.

  Recently, due to the effects of global warming, the temperature is too high, so the sap flows out, the night temperature is about minus 4 degrees, and the daytime temperature is about 4 degrees to about 9 degrees. It is difficult to pick. Therefore, a method of taking sap using a vacuum pump is also employed. For example, there is a method of collecting sap by connecting all the sugar maple trees with a vinyl tube and connecting them to a vacuum pump.

  Such a collection method can be said to be a unilateral collection of nutrients that cause damage to trees and cause tree growth.

  In the present embodiment, the sampling tube 100 is provided in a part near the tree skin of the tree, a cutout portion corresponding to the pipe joint portion 130 of the sampling tube 100 is cut out on the tree skin, and the pipe joint portion 130 of the sampling tube 100 is provided. Fit into the cutout. As a result, the sap can travel to and from the canal near the epidermis of the tree or part of the master tube via the pipe joint 130.

  As described above, the present embodiment provides a method for collecting sap by connecting the collection tube 100 to the tree so as not to impair the function of the tree. In addition, the sap can be collected in accordance with the natural growth rhythm of the tree by adjusting the collection valve, and the collected solution can be reduced (sap return).

<Example 4: Method for collecting herbal ingredients>
Plant components include primary metabolites and secondary metabolites, and organic compounds have a presence as plant components. Most of it consists of carbon, hydrogen, oxygen, and nitrogen, so if you burn it, it becomes carbon dioxide or water. However, these are all created by the substance metabolism system in the living body. This is why organic compounds produced by organisms, not limited to plants, are called “metabolites”. Biological metabolites can be broadly divided into “primary metabolite” and “secondary metabolite”.

  A primary metabolite is a group of substances essential for maintaining a living body, and exists in common for organisms belonging to each taxon. For example, high molecular compounds such as DNA, RNA, proteins, carbohydrates, and lipids and their constituent units such as nucleic acids, amino acids, monosaccharides, and fatty acids are indispensable for most organisms. In addition, lignin and cellulose, which are fibers contained in higher plants, are also basic elements of mechanical tissue and are regarded as primary metabolites.

  On the other hand, those that are derived from the primary metabolic system and are not necessarily essential for living organisms are called secondary metabolites. Secondary metabolites are not created individually by each species, but are synthesized under the control of an enzyme system in vivo according to a certain pathway (called biosynthetic pathway) with a common precursor. Therefore, the biosynthetic ability of the secondary metabolite can be regarded as a genetic trait inherent to the species, and the chemical diversity of the secondary metabolite can be said to be a product that has been built with the evolution and differentiation of the species. It can be said that the large number of secondary metabolites accumulated on the earth was created by biodiversity. In fact, some of the secondary metabolites are structurally similar or have a common partial structure, and it is possible to classify a wide variety of secondary metabolites based on this. Since ancient times, mankind has used a huge amount of chemistry as a medicine. Secondary metabolites here are based on the following two categories: biosynthetic pathways (isoprenoids, polyketides, etc.) and structurally miscellaneous. When classified and explained as having common characteristics (saponins, tannins, alkaloids, etc.), the latter is not necessarily named according to a clear protocol, and there are many things that should be called common names. There are only a few parts.

  By using the secondary metabolite as a target for collection by the collection tube 100, many medicinal herb components and the like can be collected from plants over a long period of time. Not only that, it also has the effect of strengthening and growing the ingredients of the plant itself.

<Example 5: Method for collecting metabolites from cherry tomatoes>
“We measured the xylem flow with the fruit pattern of cherry tomato (Sun cherry extra) and observed that water flowed into the fruit from night to early morning, and that water flowed out of the fruit during the day. It is inferred that the inflow of water into the fruit from night to early morning enlarges the pulp cells and is caused by the pressure, and that the promotion of transpiration from the leaves during the dark period suppresses the occurrence of raspberries. It was clarified that the rate of weight change of melon fruits and the xylem flow velocity to the fruits were almost parallel, and that water flowed back from the fruits toward the foliage when the water stress was strong in the daytime. We measured the transpiration flow at the inflorescence part of the mango inflorescence, and found that the transpiration flow in the inflorescence is not affected by solar radiation and satiety. The transpiration flow at the branch The big deal during the day recognized the flow of the branch treetops from the fruit, which it has been reported that matches well with the change date of the fruit size. "Repeating the hypertrophy and contraction.

  In addition, "Tomato was used to develop the root system from the two side branches, and the water movement between the two sides was examined as a dry soil side and a wet soil side. In such a case, when the soil was extremely dry, It has been found that about 10 cc of water moves to the dry soil side from early morning to early morning, and diurnal changes in the amount of root water absorbed from the east and west sides in the north and south ridges where tomatoes are grown. And found that the east surface is in an advantageous environment for water absorption. Measurement of the xylem flow in the stem also confirmed that the roots absorbed water at night. " .

  From these facts, when the whole plant is regarded as one huge "polynuclear body", when drying occurs in one part of the plant, the action of moisture in other parts fills that part. It can be taken. Moreover, it is thought that not only moisture but also nutrients are the same. It can be said that moisture and nutrients diffuse due to the diffusion phenomenon because the whole plant becomes a uniform solution.

  Therefore, it can be said that the whole plant individual can be controlled by controlling the solution in a part (fruit branch, stem, root) of the plant individual.

  Apples also try to drain excess moisture from the pulp. At that time, when the temperature drops, water drops on the apples. This water droplet makes it difficult for moisture to come out from the fruit, and as a result, it becomes easier to form nectar.

  Therefore, in this embodiment, by controlling the pressure (for example, hydraulic pressure) of the canal and phloem, it is possible to control the enlargement of the fruit, prevent the occurrence of crushing, and the moisture and nutrients ( Control the amount of growth hormone, etc.). As a result, it is possible to control the growth time, size, sweetness (taste), and the like of the fruit. For this reason, not only changes in substances obtained from the extraction of metabolites, but also controls metabolites contained in the plant body (for example, vegetables) and fruits obtained from plants in which this metabolite collection controller 300 is installed, It is possible to adjust the flowering of. That is, the metabolite collection controller 300 controls the state of the plant by mixing a part of the collected metabolite or the plant hormones obtained elsewhere, and returning the mixture to the plant body. ) And fruit production (taste, shape, etc.) and collected metabolites.

  As mentioned above, although the example which applied this invention with respect to some plants was demonstrated, it is not restricted to this, This invention is applied suitably according to the kind, form, property, component to collect, etc. of a plant. be able to.

  DESCRIPTION OF SYMBOLS 1 ... Plant, 1a ... Grown plant, 2 ... Stem, 10 ... Plant metabolite collection system, 11 ... Fruit, 12 ... Fruit handle, 20 ... Master tube Cells, 21 ... companion cells, 22 ... master plate, 23 ... master area, 24 ... canal, 30 ... stem, 31 ... root, 32 ... core, 33 ... Space, 34 ... Dividing point, 100 ... Collection tube, 100a ... Collection tube for road tube, 100b ... Collection tube for teacher tube, 101 ... Absorption tube, 102 ... Sending Inlet pipe, 103 ... capillary, 104 ... tip (part), 104a ... swollen sampling tube tip, 104b ... porous sampling tube tip, 105 ... osmotic membrane, 106 ...・ Porous part, 107: Incoming liquid hole network, 110: Collection tank, 110a: Collection tank for road pipes, 110b: Collection for teacher pipes , 111 ... Storage / concentration tank, 115 ... Faucet, 120 ... Permeation membrane, 130 ... Pipe joint part, 140 ... Branch part, 150 ... Cut-out part, 160 ... Insertion tube, 170 ... cavernous body, 180 ... cover part, 190 ... motorized valve, 200 ... collection fluid, 201 ... flow of collection fluid, 204 ... between companion cells and tubules Flow, 205 ... flow between trunk and capillary, 206 ... flow of conduit joint, 207 ... flow between capillary and cavernous body 210 ... delivery liquid, 211 ... sleeve, 212. ..Protective sleeve, 300 ... metabolite collection controller, 310 ... first suction pump, 320 ... semi-permeable membrane, 330 ... second suction pump, 340 ... third suction Pump, 350 ... Water feed tank, 360 ... First feed Pump ... 370 ... second delivery pump 400 ... plant battery 401 ... oxygen delivery tube 402 ... water delivery tube 403 ... glucose delivery tube 404 ... Gluconolactone delivery pipe, 410 ... separator, 500 ... adhesive tape

Claims (3)

A first collection tube connected to a xylem of the plant (path pipe, temporary path pipe);
A second sampling tube connected to the sieve portion of the plant (Shikan) ,
A plant metabolite collection device , wherein a permeable membrane is provided on a connection surface between the xylem and the sieve portion and the first collection tube and the second collection tube . A first collection tube connected to a xylem of the plant (path pipe, temporary path pipe);
A second sampling tube connected to the sieve portion of the plant (Shikan) ,
The plant metabolite collection device, wherein the first collection tube and the second collection tube have a structure in which a plurality of thin tubes or a plurality of fibers are bundled . A first collection tube connected to a xylem of the plant (path pipe, temporary path pipe);
A second sampling tube connected to the sieve portion of the plant (Shikan) ,
The first collection tube and the second collection tube each have an absorption tube for extracting a metabolite from the plant and an inlet tube for delivering the extracted metabolite to the plant. Plant metabolite collection device characterized.

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