JP5467243B2 - 可溶性羽毛ケラチン蛋白質の製造方法 - Google Patents
可溶性羽毛ケラチン蛋白質の製造方法 Download PDFInfo
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- JP5467243B2 JP5467243B2 JP2008156122A JP2008156122A JP5467243B2 JP 5467243 B2 JP5467243 B2 JP 5467243B2 JP 2008156122 A JP2008156122 A JP 2008156122A JP 2008156122 A JP2008156122 A JP 2008156122A JP 5467243 B2 JP5467243 B2 JP 5467243B2
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- keratin protein
- solution
- feather keratin
- soluble
- producing
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- 101710200191 Feather keratin Proteins 0.000 title claims description 52
- 238000004519 manufacturing process Methods 0.000 title claims description 22
- CWERGRDVMFNCDR-UHFFFAOYSA-N thioglycolic acid Chemical compound OC(=O)CS CWERGRDVMFNCDR-UHFFFAOYSA-N 0.000 claims description 66
- 210000003746 feather Anatomy 0.000 claims description 39
- 239000007800 oxidant agent Substances 0.000 claims description 23
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 22
- MHAJPDPJQMAIIY-UHFFFAOYSA-N Hydrogen peroxide Chemical compound OO MHAJPDPJQMAIIY-UHFFFAOYSA-N 0.000 claims description 18
- XUXNAKZDHHEHPC-UHFFFAOYSA-M sodium bromate Chemical compound [Na+].[O-]Br(=O)=O XUXNAKZDHHEHPC-UHFFFAOYSA-M 0.000 claims description 18
- 102000004169 proteins and genes Human genes 0.000 claims description 9
- 108090000623 proteins and genes Proteins 0.000 claims description 9
- 238000003756 stirring Methods 0.000 claims description 5
- 239000003795 chemical substances by application Substances 0.000 claims description 2
- 239000000243 solution Substances 0.000 description 55
- 239000000126 substance Substances 0.000 description 30
- 238000006243 chemical reaction Methods 0.000 description 28
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 24
- 108010076876 Keratins Proteins 0.000 description 16
- 102000011782 Keratins Human genes 0.000 description 16
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 15
- 241000287828 Gallus gallus Species 0.000 description 12
- 125000003396 thiol group Chemical group [H]S* 0.000 description 12
- 238000011282 treatment Methods 0.000 description 12
- DLLMHEDYJQACRM-UHFFFAOYSA-N 2-(carboxymethyldisulfanyl)acetic acid Chemical compound OC(=O)CSSCC(O)=O DLLMHEDYJQACRM-UHFFFAOYSA-N 0.000 description 9
- 125000002228 disulfide group Chemical group 0.000 description 8
- 235000018102 proteins Nutrition 0.000 description 8
- 125000000151 cysteine group Chemical group N[C@@H](CS)C(=O)* 0.000 description 7
- 238000000034 method Methods 0.000 description 7
- 230000007935 neutral effect Effects 0.000 description 7
- 238000007254 oxidation reaction Methods 0.000 description 7
- 238000006722 reduction reaction Methods 0.000 description 7
- 239000007864 aqueous solution Substances 0.000 description 6
- 235000013330 chicken meat Nutrition 0.000 description 6
- 239000003599 detergent Substances 0.000 description 6
- 239000002699 waste material Substances 0.000 description 6
- 238000004090 dissolution Methods 0.000 description 5
- 230000009467 reduction Effects 0.000 description 5
- 241000272814 Anser sp. Species 0.000 description 4
- LEVWYRKDKASIDU-IMJSIDKUSA-N cystine group Chemical group C([C@@H](C(=O)O)N)SSC[C@@H](C(=O)O)N LEVWYRKDKASIDU-IMJSIDKUSA-N 0.000 description 4
- 239000000835 fiber Substances 0.000 description 4
- 229920000642 polymer Polymers 0.000 description 4
- 108090000765 processed proteins & peptides Proteins 0.000 description 4
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 3
- VMHLLURERBWHNL-UHFFFAOYSA-M Sodium acetate Chemical compound [Na+].CC([O-])=O VMHLLURERBWHNL-UHFFFAOYSA-M 0.000 description 3
- 239000002253 acid Substances 0.000 description 3
- 239000003638 chemical reducing agent Substances 0.000 description 3
- 238000011033 desalting Methods 0.000 description 3
- 238000001962 electrophoresis Methods 0.000 description 3
- 239000000706 filtrate Substances 0.000 description 3
- 239000011521 glass Substances 0.000 description 3
- 238000006460 hydrolysis reaction Methods 0.000 description 3
- 230000003647 oxidation Effects 0.000 description 3
- 230000001590 oxidative effect Effects 0.000 description 3
- 238000001556 precipitation Methods 0.000 description 3
- 230000008569 process Effects 0.000 description 3
- 239000001632 sodium acetate Substances 0.000 description 3
- 235000017281 sodium acetate Nutrition 0.000 description 3
- WCRCOODEFQFSPO-VKHMYHEASA-N 2-[[(2S)-2-aminopropanoyl]disulfanyl]acetic acid Chemical group C[C@H](N)C(=O)SSCC(O)=O WCRCOODEFQFSPO-VKHMYHEASA-N 0.000 description 2
- IAZDPXIOMUYVGZ-UHFFFAOYSA-N Dimethylsulphoxide Chemical compound CS(C)=O IAZDPXIOMUYVGZ-UHFFFAOYSA-N 0.000 description 2
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 2
- 150000007513 acids Chemical class 0.000 description 2
- 239000003513 alkali Substances 0.000 description 2
- 239000002585 base Substances 0.000 description 2
- 108010049062 beta-Keratins Proteins 0.000 description 2
- 125000003178 carboxy group Chemical group [H]OC(*)=O 0.000 description 2
- 238000005119 centrifugation Methods 0.000 description 2
- 230000008859 change Effects 0.000 description 2
- 238000004132 cross linking Methods 0.000 description 2
- 235000019441 ethanol Nutrition 0.000 description 2
- 238000005194 fractionation Methods 0.000 description 2
- 238000010438 heat treatment Methods 0.000 description 2
- 230000007062 hydrolysis Effects 0.000 description 2
- 239000002244 precipitate Substances 0.000 description 2
- CRWJEUDFKNYSBX-UHFFFAOYSA-N sodium;hypobromite Chemical compound [Na+].Br[O-] CRWJEUDFKNYSBX-UHFFFAOYSA-N 0.000 description 2
- 230000007928 solubilization Effects 0.000 description 2
- 238000005063 solubilization Methods 0.000 description 2
- 230000003381 solubilizing effect Effects 0.000 description 2
- 238000006276 transfer reaction Methods 0.000 description 2
- 230000009466 transformation Effects 0.000 description 2
- 238000001291 vacuum drying Methods 0.000 description 2
- 241000271566 Aves Species 0.000 description 1
- BWGNESOTFCXPMA-UHFFFAOYSA-N Dihydrogen disulfide Chemical compound SS BWGNESOTFCXPMA-UHFFFAOYSA-N 0.000 description 1
- 108091005804 Peptidases Proteins 0.000 description 1
- 239000004365 Protease Substances 0.000 description 1
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 description 1
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 description 1
- 230000002378 acidificating effect Effects 0.000 description 1
- 235000001014 amino acid Nutrition 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 238000009835 boiling Methods 0.000 description 1
- 238000006664 bond formation reaction Methods 0.000 description 1
- 230000005587 bubbling Effects 0.000 description 1
- 239000004202 carbamide Substances 0.000 description 1
- 150000001793 charged compounds Chemical class 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- 235000018417 cysteine Nutrition 0.000 description 1
- XUJNEKJLAYXESH-UHFFFAOYSA-N cysteine Natural products SCC(N)C(O)=O XUJNEKJLAYXESH-UHFFFAOYSA-N 0.000 description 1
- 239000012153 distilled water Substances 0.000 description 1
- 239000003337 fertilizer Substances 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 238000004108 freeze drying Methods 0.000 description 1
- 238000002523 gelfiltration Methods 0.000 description 1
- 150000002500 ions Chemical class 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 230000000269 nucleophilic effect Effects 0.000 description 1
- 239000012460 protein solution Substances 0.000 description 1
- 238000007348 radical reaction Methods 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 230000035484 reaction time Effects 0.000 description 1
- 230000009257 reactivity Effects 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 238000002415 sodium dodecyl sulfate polyacrylamide gel electrophoresis Methods 0.000 description 1
- GNBVPFITFYNRCN-UHFFFAOYSA-M sodium thioglycolate Chemical compound [Na+].[O-]C(=O)CS GNBVPFITFYNRCN-UHFFFAOYSA-M 0.000 description 1
- 229940046307 sodium thioglycolate Drugs 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 125000001424 substituent group Chemical group 0.000 description 1
- 150000005846 sugar alcohols Polymers 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/78—Connective tissue peptides, e.g. collagen, elastin, laminin, fibronectin, vitronectin or cold insoluble globulin [CIG]
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08H—DERIVATIVES OF NATURAL MACROMOLECULAR COMPOUNDS
- C08H1/00—Macromolecular products derived from proteins
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08L—COMPOSITIONS OF MACROMOLECULAR COMPOUNDS
- C08L89/00—Compositions of proteins; Compositions of derivatives thereof
- C08L89/04—Products derived from waste materials, e.g. horn, hoof or hair
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Medicinal Chemistry (AREA)
- Polymers & Plastics (AREA)
- Biochemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Materials Engineering (AREA)
- Engineering & Computer Science (AREA)
- Toxicology (AREA)
- Zoology (AREA)
- Gastroenterology & Hepatology (AREA)
- Biophysics (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Peptides Or Proteins (AREA)
Description
(A)還元剤を含むあるいは含まない非アルカリ性水溶液に羽毛を浸漬し、それを0.2〜0.32MPaに加圧しながら121〜136℃の高温にすることで、羽毛を軟化させる工程。
(B)(A)工程により得られた羽毛含有非アルカリ水溶液のpHを、塩基を添加してアルカリに調整した後、プロテアーゼを添加して加水分解反応させることにより、軟化させた羽毛を部分的に消化させる工程。
(C)(B)行程により得られた部分的に消化された羽毛を、(I)親水性の置換基で化学修飾する工程、及び/または(II)尿素、アルコール、多価アルコールから選ばれる1種以上を含有する水溶液に高分子のまま可溶化せしめる溶解工程。
第1工程で得られた溶液をpH5〜8に調整し、この溶液に酸化剤を加えて3〜10時間放置することにより可溶性羽毛ケラチン蛋白質を製造する第2工程と、
による可溶性羽毛ケラチン蛋白質の製造方法である。
まず該羽毛を水に浸漬する。この水は重量比で該羽毛の20〜200倍とする。他方、チオグリコール酸溶液をアルカリ性に調整し、これを上記水の中に添加する。このチオグリコール酸溶液濃度は、0.05〜5mol/l程度となるように、チオグリコール酸の溶解量を定めておく。またこのチオグリコール酸溶液はpH8.0〜13に調整しておくものであるが、そのために種々の塩基を自由に採用することができる。例えば、ナトリウムの水酸化物を採用することができる。このように羽毛を浸漬した水にアルカリ性に調整したチオグリコール酸を添加してこれを攪拌振とうする。この攪拌振とうは、この溶液を20〜50℃に保持しながら1/3〜120時間行う。
Claims (4)
- 鳥類の羽毛を重量比で20〜200倍の水に浸漬し、該羽毛を浸漬した水に、チオグリコール酸(TGA)溶液濃度が0.05〜5mol/lとなるように、pH8.0〜13に調整したチオグリコール酸溶液を加え、かつ該チオグリコール酸溶液を加えた水を20〜50℃に保持しながら1/3〜120時間攪拌振とうする第1工程(蛋白質変性剤を使用する場合を除く)と、
第1工程で得られた溶液をpH5〜8に調整し、この溶液に酸化剤を加えて3〜10時間放置することにより可溶性羽毛ケラチン蛋白質を製造する第2工程と、
による可溶性羽毛ケラチン蛋白質の製造方法。 - 前記第2工程の酸化剤として、過酸化水素、臭素酸ソーダ又は空気を用いた請求項1の可溶性羽毛ケラチン蛋白質の製造方法。
- 前記第2工程の酸化剤として過酸化水素又は臭素酸ソーダを用い、該過酸化水素又は臭素酸ソーダを前記放置する時間の内の前半の一定時間の間に継続して徐々に添加することとした請求項1の可溶性羽毛ケラチン蛋白質の製造方法。
- 前記第2工程の酸化剤として空気を用い、該空気を前記放置する全時間中に継続的にバブリングすることで添加することとした請求項1の可溶性羽毛ケラチン蛋白質の製造方法。
Priority Applications (2)
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JP2008156122A JP5467243B2 (ja) | 2008-06-16 | 2008-06-16 | 可溶性羽毛ケラチン蛋白質の製造方法 |
PCT/JP2009/002451 WO2009153923A1 (ja) | 2008-06-16 | 2009-06-02 | 可溶性羽毛ケラチン蛋白質の製造方法 |
Applications Claiming Priority (1)
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JP2008156122A JP5467243B2 (ja) | 2008-06-16 | 2008-06-16 | 可溶性羽毛ケラチン蛋白質の製造方法 |
Publications (3)
Publication Number | Publication Date |
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JP2009298737A JP2009298737A (ja) | 2009-12-24 |
JP2009298737A5 JP2009298737A5 (ja) | 2010-04-15 |
JP5467243B2 true JP5467243B2 (ja) | 2014-04-09 |
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JP2008156122A Active JP5467243B2 (ja) | 2008-06-16 | 2008-06-16 | 可溶性羽毛ケラチン蛋白質の製造方法 |
Country Status (2)
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JP (1) | JP5467243B2 (ja) |
WO (1) | WO2009153923A1 (ja) |
Families Citing this family (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP5411655B2 (ja) * | 2008-12-03 | 2014-02-12 | 株式会社ミルボン | 毛髪処理方法及び毛髪処理剤 |
TWI523664B (zh) * | 2009-06-12 | 2016-03-01 | Milbon Co Ltd | Hair treatment method, hair care agent and hair care preparation method |
JP2011144127A (ja) * | 2010-01-13 | 2011-07-28 | Milbon Co Ltd | 毛髪処理剤及び毛髪処理剤用原料 |
US8575313B2 (en) | 2010-11-19 | 2013-11-05 | Universiti Malaysia Pahang | Process for extracting keratin |
Family Cites Families (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPH07126296A (ja) * | 1993-05-24 | 1995-05-16 | Kao Corp | 可溶化蛋白質 |
JPH092919A (ja) * | 1995-06-19 | 1997-01-07 | Kao Corp | 新規ゲル化剤及びゲル組成物 |
JP2007176863A (ja) * | 2005-12-28 | 2007-07-12 | Gunei Shoji Kk | 可溶化蛋白質の製造方法 |
-
2008
- 2008-06-16 JP JP2008156122A patent/JP5467243B2/ja active Active
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2009
- 2009-06-02 WO PCT/JP2009/002451 patent/WO2009153923A1/ja active Application Filing
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Publication number | Publication date |
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JP2009298737A (ja) | 2009-12-24 |
WO2009153923A1 (ja) | 2009-12-23 |
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