JP5452231B2 - 化学的に修飾した正常な細胞由来の抗腫瘍ワクチン - Google Patents
化学的に修飾した正常な細胞由来の抗腫瘍ワクチン Download PDFInfo
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| US8467973B2 (en) | 2009-07-28 | 2013-06-18 | Promising Future, Llc | Pairing processes for preparing alloreactive cytotoxic T cells |
| US8666674B2 (en) | 2009-07-28 | 2014-03-04 | Promising Future, Llc | Pairing processes for preparing reactive cytotoxic T cells |
| US9211321B2 (en) | 2009-10-27 | 2015-12-15 | Immunicum Ab | Method for proliferation of antigen-specific T cells |
| GB201013443D0 (en) | 2010-08-11 | 2010-09-22 | Cytovac As | Compositions and methods for producing dendritic cells |
| US10426740B1 (en) | 2010-08-18 | 2019-10-01 | Avm Biotechnology, Llc | Compositions and methods to inhibit stem cell and progenitor cell binding to lymphoid tissue and for regenerating germinal centers in lymphatic tissues |
| EA201792642A1 (ru) | 2015-05-28 | 2018-10-31 | Кайт Фарма, Инк. | Способы диагностики для т-клеточной терапии |
| IL312426B1 (en) | 2015-05-28 | 2025-10-01 | Kite Pharma Inc | Methods for training patients for T-cell therapy |
| TW202521141A (zh) | 2015-10-20 | 2025-06-01 | 美商凱特製藥公司 | 製備用於ｔ細胞治療之ｔ細胞的方法 |
| UA128781C2 (uk) | 2016-04-01 | 2024-10-23 | Кайт Фарма, Інк. | Т-клітина, спосіб її отримання та застосування |
| CU20180121A7 (es) | 2016-04-01 | 2019-05-03 | Kite Pharma Inc | Receptores de antígenos quiméricos y células t |
| KR102522622B1 (ko) | 2016-04-01 | 2023-04-18 | 카이트 파마 인코포레이티드 | Bcma 결합 분자 및 그의 사용 방법 |
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| US20180280437A1 (en) | 2017-03-13 | 2018-10-04 | Kite Pharma, Inc. | Chimeric antigen receptors for melanoma and uses thereof |
| BR112019020214A2 (pt) | 2017-04-01 | 2020-04-22 | Avm Biotechnology Llc | substituição do pré-condicionamento citotóxico antes da imunoterapia celular |
| CN110494158A (zh) | 2017-04-03 | 2019-11-22 | 凯德药业股份有限公司 | 使用包含优化的多功能t细胞的嵌合受体t细胞进行治疗 |
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| CN110662831A (zh) | 2017-05-26 | 2020-01-07 | 凯德药业股份有限公司 | 制备和使用胚胎间充质祖细胞的方法 |
| SG11202002327PA (en) | 2017-09-15 | 2020-04-29 | Kite Pharma Inc | Methods and systems for performing a patient-specific immunotherapy procedure with chain-of-custody and chain-of-identity biological sample tracking |
| WO2019060695A1 (en) | 2017-09-22 | 2019-03-28 | Kite Pharma, Inc. | CHIMERIC POLYPEPTIDES AND USES THEREOF |
| WO2019079564A1 (en) | 2017-10-18 | 2019-04-25 | Kite Pharma, Inc. | METHODS OF DELIVERY OF CHIMERIC ANTIGEN RECEPTOR IMMUNOTHERAPY |
| US11390655B2 (en) | 2017-11-16 | 2022-07-19 | Kite Pharma, Inc. | Modified chimeric antigen receptors and methods of use |
| TW201932593A (zh) | 2018-01-15 | 2019-08-16 | 美商輝瑞大藥廠 | 合併投與嵌合抗原受體之免疫療法與4-1bb促效劑的方法 |
| US12338459B2 (en) | 2018-02-02 | 2025-06-24 | Arizona Board Of Regents On Behalf Of Arizona State University | DNA-chimeric antigen receptor T cells for immunotherapy |
| KR102618231B1 (ko) | 2018-02-16 | 2023-12-28 | 카이트 파마 인코포레이티드 | 변형된 만능성 줄기 세포, 및 제조 및 사용 방법 |
| AU2019252944B2 (en) | 2018-04-12 | 2022-12-08 | Kite Pharma, Inc. | Chimeric receptor T cell treatment using characteristics of the tumor microenvironment |
| KR102734592B1 (ko) | 2018-06-01 | 2024-11-27 | 카이트 파마 인코포레이티드 | 키메라 항원 수용체 t 세포 요법 |
| US20210155941A1 (en) | 2018-06-22 | 2021-05-27 | Kite Pharma Eu B.V. | Compositions and methods for making engineered t cells |
| EP3830125A1 (en) | 2018-08-02 | 2021-06-09 | Kite Pharma, Inc. | Chimeric antigen receptor therapy t cell expansion kinetics and uses thereof |
| EP3488851A1 (en) | 2018-10-03 | 2019-05-29 | AVM Biotechnology, LLC | Immunoablative therapies |
| EP3632446B3 (en) | 2018-10-03 | 2024-01-24 | AVM Biotechnology, LLC | Immunoablative therapies |
| MX2021006968A (es) | 2018-12-12 | 2021-10-13 | Kite Pharma Inc | Antígeno quimérico y receptores de las células t, y métodos de uso. |
| WO2020208054A1 (en) | 2019-04-12 | 2020-10-15 | Cytovac A/S | Method for preparation of cancer/testis antigen-specific t-cells |
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| CN114391021A (zh) | 2019-06-21 | 2022-04-22 | 凯德药业股份有限公司 | TGF-β受体和使用方法 |
| WO2021046134A1 (en) | 2019-09-03 | 2021-03-11 | Allogene Therapeutics, Inc. | Methods of preparing t cells for t cell therapy |
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| US20250382383A1 (en) | 2024-05-31 | 2025-12-18 | Kite Pharma, Inc. | Tricistronic constructs for anti-gpc3 car |
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| AU2008203730A1 (en) | 2008-07-10 |
| GB0700058D0 (en) | 2007-02-07 |
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