JP5399919B2 - オリゴヌクレオチドを合成及び精製するための化合物及び方法 - Google Patents

オリゴヌクレオチドを合成及び精製するための化合物及び方法 Download PDF

Info

Publication number
JP5399919B2
JP5399919B2 JP2009541902A JP2009541902A JP5399919B2 JP 5399919 B2 JP5399919 B2 JP 5399919B2 JP 2009541902 A JP2009541902 A JP 2009541902A JP 2009541902 A JP2009541902 A JP 2009541902A JP 5399919 B2 JP5399919 B2 JP 5399919B2
Authority
JP
Japan
Prior art keywords
oligonucleotide
group
compound
formula
fluorous
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
JP2009541902A
Other languages
English (en)
Japanese (ja)
Other versions
JP2010513358A (ja
JP2010513358A5 (enExample
Inventor
ピー． グプタ，アマー
ジー． ウィル，スティーブン
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
F Hoffmann La Roche AG
Original Assignee
F Hoffmann La Roche AG
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by F Hoffmann La Roche AG filed Critical F Hoffmann La Roche AG
Publication of JP2010513358A publication Critical patent/JP2010513358A/ja
Publication of JP2010513358A5 publication Critical patent/JP2010513358A5/ja
Application granted granted Critical
Publication of JP5399919B2 publication Critical patent/JP5399919B2/ja
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Images

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07F—ACYCLIC, CARBOCYCLIC OR HETEROCYCLIC COMPOUNDS CONTAINING ELEMENTS OTHER THAN CARBON, HYDROGEN, HALOGEN, OXYGEN, NITROGEN, SULFUR, SELENIUM OR TELLURIUM
    • C07F9/00—Compounds containing elements of Groups 5 or 15 of the Periodic Table
    • C07F9/02—Phosphorus compounds
    • C07F9/06—Phosphorus compounds without P—C bonds
    • C07F9/22—Amides of acids of phosphorus
    • C07F9/24—Esteramides
    • C07F9/2404—Esteramides the ester moiety containing a substituent or a structure which is considered as characteristic
    • C07F9/2408—Esteramides the ester moiety containing a substituent or a structure which is considered as characteristic of hydroxyalkyl compounds

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Saccharide Compounds (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
JP2009541902A 2006-12-22 2007-12-21 オリゴヌクレオチドを合成及び精製するための化合物及び方法 Active JP5399919B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US87173306P 2006-12-22 2006-12-22
US60/871,733 2006-12-22
PCT/EP2007/011315 WO2008077600A1 (en) 2006-12-22 2007-12-21 Compounds and methods for synthesis and purification of oligonucleotides

Publications (3)

Publication Number Publication Date
JP2010513358A JP2010513358A (ja) 2010-04-30
JP2010513358A5 JP2010513358A5 (enExample) 2011-02-03
JP5399919B2 true JP5399919B2 (ja) 2014-01-29

Family

ID=39315090

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2009541902A Active JP5399919B2 (ja) 2006-12-22 2007-12-21 オリゴヌクレオチドを合成及び精製するための化合物及び方法

Country Status (7)

Country Link
US (1) US7858772B2 (enExample)
EP (1) EP2097427B1 (enExample)
JP (1) JP5399919B2 (enExample)
CN (1) CN101547928B (enExample)
CA (1) CA2671351C (enExample)
ES (1) ES2399363T3 (enExample)
WO (1) WO2008077600A1 (enExample)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20060178507A1 (en) * 2004-12-30 2006-08-10 Berry & Associates, Inc. Fluorous oligonucleotide reagents and affinity purification of oligonucleotides
US9366938B1 (en) 2009-02-17 2016-06-14 Vescent Photonics, Inc. Electro-optic beam deflector device
EP2508530A1 (en) * 2011-03-28 2012-10-10 Rheinische Friedrich-Wilhelms-Universität Bonn Purification of triphosphorylated oligonucleotides using capture tags
WO2013036846A2 (en) * 2011-09-09 2013-03-14 Koronis Pharmaceuticals, Incorporated N4 derivatives of deoxycytidine prodrugs
EP2599785A1 (en) * 2011-11-30 2013-06-05 Agilent Technologies, Inc. Novel methods for the synthesis and purification of oligomers
EP2712870A1 (en) 2012-09-27 2014-04-02 Rheinische Friedrich-Wilhelms-Universität Bonn Novel RIG-I ligands and methods for producing them
FR3020071B1 (fr) * 2014-04-17 2017-12-22 Dna Script Procede de synthese d'acides nucleiques, notamment d'acides nucleiques de grande longueur, utilisation du procede et kit pour la mise en œuvre du procede
FR3025201B1 (fr) 2014-09-02 2018-10-12 Dna Script Nucleotides modifies pour la synthese d'acides nucleiques, un kit renfermant de tels nucleotides et leur utilisation pour la production de genes ou sequences d'acides nucleiques synthetiques
EP3668883A1 (en) 2017-08-18 2020-06-24 Agilent Technologies, Inc. Orthoester compositions for affinity purification of oligonucleotides
WO2019131719A1 (ja) 2017-12-27 2019-07-04 神戸天然物化学株式会社 高脂溶性ホスホラミダイトの製造
CA3151411A1 (en) * 2019-09-23 2021-04-01 Adrian Horgan Increasing long-sequence yields in template-free enzymatic synthesis of polynucleotides

Family Cites Families (20)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4469863A (en) * 1980-11-12 1984-09-04 Ts O Paul O P Nonionic nucleic acid alkyl and aryl phosphonates and processes for manufacture and use thereof
US5235033A (en) * 1985-03-15 1993-08-10 Anti-Gene Development Group Alpha-morpholino ribonucleoside derivatives and polymers thereof
US5034506A (en) * 1985-03-15 1991-07-23 Anti-Gene Development Group Uncharged morpholino-based polymers having achiral intersubunit linkages
DE3529478A1 (de) * 1985-08-16 1987-02-19 Boehringer Mannheim Gmbh 7-desaza-2'desoxyguanosin-nukleotide, verfahren zu deren herstellung und deren verwendung zur nukleinsaeure-sequenzierung
US4816571A (en) * 1987-06-04 1989-03-28 Applied Biosystems, Inc. Chemical capping by phosphitylation during oligonucleotide synthesis
US5216141A (en) * 1988-06-06 1993-06-01 Benner Steven A Oligonucleotide analogs containing sulfur linkages
US5386023A (en) * 1990-07-27 1995-01-31 Isis Pharmaceuticals Backbone modified oligonucleotide analogs and preparation thereof through reductive coupling
US5602240A (en) * 1990-07-27 1997-02-11 Ciba Geigy Ag. Backbone modified oligonucleotide analogs
JPH04210695A (ja) * 1990-12-14 1992-07-31 Shin Etsu Chem Co Ltd アミノホスファゼン化合物の製造方法
US5644048A (en) * 1992-01-10 1997-07-01 Isis Pharmaceuticals, Inc. Process for preparing phosphorothioate oligonucleotides
US5637684A (en) * 1994-02-23 1997-06-10 Isis Pharmaceuticals, Inc. Phosphoramidate and phosphorothioamidate oligomeric compounds
AU5318398A (en) * 1996-11-07 1998-05-29 Novartis Ag Process for the preparation of an oligomeric compound
US6326478B1 (en) * 1998-07-08 2001-12-04 Isis Pharmaceuticals, Inc. Process for the synthesis of oligomeric compounds
US6222030B1 (en) * 1998-08-03 2001-04-24 Agilent Technologies, Inc. Solid phase synthesis of oligonucleotides using carbonate protecting groups and alpha-effect nucleophile deprotection
EP1176151B1 (en) * 2000-07-28 2014-08-20 Agilent Technologies, Inc. Synthesis of polynucleotides using combined oxidation/deprotection chemistry
US7098326B2 (en) 2002-01-23 2006-08-29 Sigma-Aldrich Co. Methods for the integrated synthesis and purification of oligonucleotides
WO2004063208A1 (en) * 2003-01-09 2004-07-29 Postech Foundation New phosphoramidite compounds
WO2006047628A2 (en) * 2004-10-25 2006-05-04 Operon Holdings, Inc. Amino or thiol linker building block for the synthesis of amino- or thiol-functionalized nucleic acids and methods of making and use thereof
US20060178507A1 (en) 2004-12-30 2006-08-10 Berry & Associates, Inc. Fluorous oligonucleotide reagents and affinity purification of oligonucleotides
KR101151159B1 (ko) * 2006-09-19 2012-06-01 삼성전자주식회사 포스페이트계 자기조립단분자막을 포함하는 유기 박막트랜지스터 및 그 제조방법

Also Published As

Publication number Publication date
JP2010513358A (ja) 2010-04-30
US20080161548A1 (en) 2008-07-03
US7858772B2 (en) 2010-12-28
EP2097427A1 (en) 2009-09-09
WO2008077600A1 (en) 2008-07-03
HK1134295A1 (en) 2010-04-23
ES2399363T3 (es) 2013-03-27
CA2671351A1 (en) 2008-07-03
WO2008077600B1 (en) 2008-09-04
CN101547928A (zh) 2009-09-30
EP2097427B1 (en) 2012-11-21
CA2671351C (en) 2012-10-30
CN101547928B (zh) 2013-01-02

Similar Documents

Publication Publication Date Title
JP5399919B2 (ja) オリゴヌクレオチドを合成及び精製するための化合物及び方法
KR102640410B1 (ko) 표면-결합 올리고뉴클레오타이드의 화학적 절단 및 탈보호를 위한 조성물 및 방법
EP0543889B1 (en) Incorporation of selectably clevable sites into oligonucleotide chains and reagents therefor
US5367066A (en) Oligonucleotides with selectably cleavable and/or abasic sites
US5258506A (en) Photolabile reagents for incorporation into oligonucleotide chains
CA2368135C (en) Xylo-lna analogues
US7084125B2 (en) Xylo-LNA analogues
KR101032008B1 (ko) 폴리뉴클레오티드 표지 시약
US6451998B1 (en) Capping and de-capping during oligonucleotide synthesis
EP0090789A1 (en) Chemical DNA synthesis
WO2002020537A2 (en) Linker phosphoramidites for oligonucleotide synthesis
AU8650991A (en) Synthesis of oligonucleotides
EP0595839A4 (enExample)
JPS59502025A (ja) オリゴヌクレオシドホスホネ−トの製造法
US20030195351A1 (en) Methods for the integrated synthesis and purification of oligonucleotides
HK1134295B (en) Compounds and methods for synthesis and purification of oligonucleotides
Mori et al. Synthesis and hybridization property of a boat-shaped pyranosyl nucleic acid containing an exocyclic methylene group in the sugar moiety
KR20240131337A (ko) 폴리뉴클레오티드 제조 방법
CA3185976A1 (en) Fluorescent dyes
Leuck Pieken et al.(45) Date of Patent: Aug. 29, 2006
IE912661A1 (en) Incorporation of selectably cleavable and/or abasic sites¹into oligonulceotide chains and reagents therefor

Legal Events

Date Code Title Description
A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20101208

A621 Written request for application examination

Free format text: JAPANESE INTERMEDIATE CODE: A621

Effective date: 20101208

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20130219

A601 Written request for extension of time

Free format text: JAPANESE INTERMEDIATE CODE: A601

Effective date: 20130509

A602 Written permission of extension of time

Free format text: JAPANESE INTERMEDIATE CODE: A602

Effective date: 20130516

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20130819

TRDD Decision of grant or rejection written
A01 Written decision to grant a patent or to grant a registration (utility model)

Free format text: JAPANESE INTERMEDIATE CODE: A01

Effective date: 20130924

A61 First payment of annual fees (during grant procedure)

Free format text: JAPANESE INTERMEDIATE CODE: A61

Effective date: 20131024

R150 Certificate of patent or registration of utility model

Ref document number: 5399919

Country of ref document: JP

Free format text: JAPANESE INTERMEDIATE CODE: R150

Free format text: JAPANESE INTERMEDIATE CODE: R150

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250