CN101547928B - 合成和纯化寡核苷酸的化合物和方法 - Google Patents

合成和纯化寡核苷酸的化合物和方法 Download PDF

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Publication number
CN101547928B
CN101547928B CN200780044741.8A CN200780044741A CN101547928B CN 101547928 B CN101547928 B CN 101547928B CN 200780044741 A CN200780044741 A CN 200780044741A CN 101547928 B CN101547928 B CN 101547928B
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oligonucleotide
compound
oligonucleotides
capping
group
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Chinese (zh)
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CN101547928A (zh
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A·P·古普塔
S·G·威尔
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F Hoffmann La Roche AG
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F Hoffmann La Roche AG
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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07F—ACYCLIC, CARBOCYCLIC OR HETEROCYCLIC COMPOUNDS CONTAINING ELEMENTS OTHER THAN CARBON, HYDROGEN, HALOGEN, OXYGEN, NITROGEN, SULFUR, SELENIUM OR TELLURIUM
    • C07F9/00—Compounds containing elements of Groups 5 or 15 of the Periodic Table
    • C07F9/02—Phosphorus compounds
    • C07F9/06—Phosphorus compounds without P—C bonds
    • C07F9/22—Amides of acids of phosphorus
    • C07F9/24—Esteramides
    • C07F9/2404—Esteramides the ester moiety containing a substituent or a structure which is considered as characteristic
    • C07F9/2408—Esteramides the ester moiety containing a substituent or a structure which is considered as characteristic of hydroxyalkyl compounds

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Saccharide Compounds (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
CN200780044741.8A 2006-12-22 2007-12-21 合成和纯化寡核苷酸的化合物和方法 Active CN101547928B (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US87173306P 2006-12-22 2006-12-22
US60/871,733 2006-12-22
PCT/EP2007/011315 WO2008077600A1 (en) 2006-12-22 2007-12-21 Compounds and methods for synthesis and purification of oligonucleotides

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CN101547928A CN101547928A (zh) 2009-09-30
CN101547928B true CN101547928B (zh) 2013-01-02

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US (1) US7858772B2 (enExample)
EP (1) EP2097427B1 (enExample)
JP (1) JP5399919B2 (enExample)
CN (1) CN101547928B (enExample)
CA (1) CA2671351C (enExample)
ES (1) ES2399363T3 (enExample)
WO (1) WO2008077600A1 (enExample)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20060178507A1 (en) * 2004-12-30 2006-08-10 Berry & Associates, Inc. Fluorous oligonucleotide reagents and affinity purification of oligonucleotides
US9366938B1 (en) 2009-02-17 2016-06-14 Vescent Photonics, Inc. Electro-optic beam deflector device
EP2508530A1 (en) 2011-03-28 2012-10-10 Rheinische Friedrich-Wilhelms-Universität Bonn Purification of triphosphorylated oligonucleotides using capture tags
WO2013036846A2 (en) * 2011-09-09 2013-03-14 Koronis Pharmaceuticals, Incorporated N4 derivatives of deoxycytidine prodrugs
JP2013151468A (ja) * 2011-11-30 2013-08-08 Agilent Technologies Inc オリゴマーの合成及び精製の新規方法
EP2712870A1 (en) 2012-09-27 2014-04-02 Rheinische Friedrich-Wilhelms-Universität Bonn Novel RIG-I ligands and methods for producing them
FR3020071B1 (fr) * 2014-04-17 2017-12-22 Dna Script Procede de synthese d'acides nucleiques, notamment d'acides nucleiques de grande longueur, utilisation du procede et kit pour la mise en œuvre du procede
FR3025201B1 (fr) 2014-09-02 2018-10-12 Dna Script Nucleotides modifies pour la synthese d'acides nucleiques, un kit renfermant de tels nucleotides et leur utilisation pour la production de genes ou sequences d'acides nucleiques synthetiques
US11299483B2 (en) 2017-08-18 2022-04-12 Agilent Technologies, Inc. Orthoester compositions for affinity purification of oligonucleotides
EP3733680A4 (en) 2017-12-27 2021-12-29 KNC Laboratories Co., Ltd Production of highly fat-soluble phosphoramidite
JP2022549196A (ja) * 2019-09-23 2022-11-24 ディーエヌエー スクリプト ポリヌクレオチドの鋳型なし酵素的合成における長配列収率の増加

Citations (2)

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US20020055623A1 (en) * 1998-07-08 2002-05-09 Isis Pharmaceuticals. Inc. Process for the synthesis of oligomeric compounds
WO2006081035A2 (en) * 2004-12-30 2006-08-03 Berry & Associates, Inc. Fluorous oligonucleotide reagents and affinity purification of oligonucleotides

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US4469863A (en) * 1980-11-12 1984-09-04 Ts O Paul O P Nonionic nucleic acid alkyl and aryl phosphonates and processes for manufacture and use thereof
US5034506A (en) * 1985-03-15 1991-07-23 Anti-Gene Development Group Uncharged morpholino-based polymers having achiral intersubunit linkages
US5235033A (en) * 1985-03-15 1993-08-10 Anti-Gene Development Group Alpha-morpholino ribonucleoside derivatives and polymers thereof
DE3529478A1 (de) * 1985-08-16 1987-02-19 Boehringer Mannheim Gmbh 7-desaza-2'desoxyguanosin-nukleotide, verfahren zu deren herstellung und deren verwendung zur nukleinsaeure-sequenzierung
US4816571A (en) * 1987-06-04 1989-03-28 Applied Biosystems, Inc. Chemical capping by phosphitylation during oligonucleotide synthesis
US5216141A (en) * 1988-06-06 1993-06-01 Benner Steven A Oligonucleotide analogs containing sulfur linkages
US5602240A (en) * 1990-07-27 1997-02-11 Ciba Geigy Ag. Backbone modified oligonucleotide analogs
US5386023A (en) * 1990-07-27 1995-01-31 Isis Pharmaceuticals Backbone modified oligonucleotide analogs and preparation thereof through reductive coupling
JPH04210695A (ja) * 1990-12-14 1992-07-31 Shin Etsu Chem Co Ltd アミノホスファゼン化合物の製造方法
US5644048A (en) * 1992-01-10 1997-07-01 Isis Pharmaceuticals, Inc. Process for preparing phosphorothioate oligonucleotides
US5637684A (en) * 1994-02-23 1997-06-10 Isis Pharmaceuticals, Inc. Phosphoramidate and phosphorothioamidate oligomeric compounds
US6107479A (en) * 1996-11-07 2000-08-22 Novartis Ag Process for the preparation of an oligomeric compound
US6222030B1 (en) * 1998-08-03 2001-04-24 Agilent Technologies, Inc. Solid phase synthesis of oligonucleotides using carbonate protecting groups and alpha-effect nucleophile deprotection
US7135565B2 (en) * 2000-07-28 2006-11-14 Agilent Technologies, Inc. Synthesis of polynucleotides using combined oxidation/deprotection chemistry
US7098326B2 (en) 2002-01-23 2006-08-29 Sigma-Aldrich Co. Methods for the integrated synthesis and purification of oligonucleotides
US20070015927A1 (en) * 2003-01-09 2007-01-18 Kim Byeang H New phosphoramidite compounds
JP2008517950A (ja) * 2004-10-25 2008-05-29 オペロン ホールディングス， インコーポレイテッド アミノ官能基化またはチオール官能基化された核酸の合成のためのアミノリンカーまたはチオールリンカーの構築ブロック、並びにこれらを作製および使用する方法
KR101151159B1 (ko) * 2006-09-19 2012-06-01 삼성전자주식회사 포스페이트계 자기조립단분자막을 포함하는 유기 박막트랜지스터 및 그 제조방법

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20020055623A1 (en) * 1998-07-08 2002-05-09 Isis Pharmaceuticals. Inc. Process for the synthesis of oligomeric compounds
WO2006081035A2 (en) * 2004-12-30 2006-08-03 Berry & Associates, Inc. Fluorous oligonucleotide reagents and affinity purification of oligonucleotides

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
christian beller, et al..Noncovalent attachment of nucleotides by fluorous-fluorous interactions:application to a simple purification principle for synthetic DNA fragments.《Helvetica chimica acta》.2005,第88卷第171-179页. *
Mallinath B. Hadimani, et al..Synthesis, In Vitro, and In Vivo Evaluation of Phosphate Ester Derivatives of Combretastatin A-4.《Bioorganic & Medicinal Chemistry Letters》.2003,第13卷第1505–1508页. *
William H. Pearson,et al..Fluorous Affinity Purification of Oligonucleotides.《J. Org. Chem.》.2005,第70卷(第18期),第7114-7122页. *

Also Published As

Publication number Publication date
CN101547928A (zh) 2009-09-30
WO2008077600B1 (en) 2008-09-04
US7858772B2 (en) 2010-12-28
EP2097427B1 (en) 2012-11-21
WO2008077600A1 (en) 2008-07-03
JP2010513358A (ja) 2010-04-30
ES2399363T3 (es) 2013-03-27
HK1134295A1 (en) 2010-04-23
CA2671351C (en) 2012-10-30
EP2097427A1 (en) 2009-09-09
US20080161548A1 (en) 2008-07-03
CA2671351A1 (en) 2008-07-03
JP5399919B2 (ja) 2014-01-29

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