JP5306811B2 - ２本鎖ｄｎａへの高い選択性を有する蛍光化学物質及びそれらの使用 - Google Patents

２本鎖ｄｎａへの高い選択性を有する蛍光化学物質及びそれらの使用 Download PDF

Info

Publication number
JP5306811B2
JP5306811B2 JP2008511465A JP2008511465A JP5306811B2 JP 5306811 B2 JP5306811 B2 JP 5306811B2 JP 2008511465 A JP2008511465 A JP 2008511465A JP 2008511465 A JP2008511465 A JP 2008511465A JP 5306811 B2 JP5306811 B2 JP 5306811B2
Authority
JP
Japan
Prior art keywords
compound
group
preparation
alkyl
sample
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
JP2008511465A
Other languages
English (en)
Japanese (ja)
Other versions
JP2008540578A (ja
Inventor
ジョレン エイ． ブラッドフォード
チン―イン チュン
シウ―ジュン ファン
パトリック アール． ピンソン
ステファン ティ． ユエ
Original Assignee
ライフ テクノロジーズ コーポレーション
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by ライフ テクノロジーズ コーポレーション filed Critical ライフ テクノロジーズ コーポレーション
Publication of JP2008540578A publication Critical patent/JP2008540578A/ja
Application granted granted Critical
Publication of JP5306811B2 publication Critical patent/JP5306811B2/ja
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/582—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with fluorescent label
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D215/00—Heterocyclic compounds containing quinoline or hydrogenated quinoline ring systems
    • C07D215/02—Heterocyclic compounds containing quinoline or hydrogenated quinoline ring systems having no bond between the ring nitrogen atom and a non-ring member or having only hydrogen atoms or carbon atoms directly attached to the ring nitrogen atom
    • C07D215/04—Heterocyclic compounds containing quinoline or hydrogenated quinoline ring systems having no bond between the ring nitrogen atom and a non-ring member or having only hydrogen atoms or carbon atoms directly attached to the ring nitrogen atom with only hydrogen atoms or radicals containing only hydrogen and carbon atoms, directly attached to the ring carbon atoms
    • C07D215/10—Quaternary compounds
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D215/00—Heterocyclic compounds containing quinoline or hydrogenated quinoline ring systems
    • C07D215/02—Heterocyclic compounds containing quinoline or hydrogenated quinoline ring systems having no bond between the ring nitrogen atom and a non-ring member or having only hydrogen atoms or carbon atoms directly attached to the ring nitrogen atom
    • C07D215/16—Heterocyclic compounds containing quinoline or hydrogenated quinoline ring systems having no bond between the ring nitrogen atom and a non-ring member or having only hydrogen atoms or carbon atoms directly attached to the ring nitrogen atom with hetero atoms or with carbon atoms having three bonds to hetero atoms with at the most one bond to halogen, e.g. ester or nitrile radicals, directly attached to ring carbon atoms
    • C07D215/20—Oxygen atoms
    • C07D215/22—Oxygen atoms attached in position 2 or 4
    • C07D215/227—Oxygen atoms attached in position 2 or 4 only one oxygen atom which is attached in position 2
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D277/00—Heterocyclic compounds containing 1,3-thiazole or hydrogenated 1,3-thiazole rings
    • C07D277/60—Heterocyclic compounds containing 1,3-thiazole or hydrogenated 1,3-thiazole rings condensed with carbocyclic rings or ring systems
    • C07D277/62—Benzothiazoles
    • C07D277/68—Benzothiazoles with hetero atoms or with carbon atoms having three bonds to hetero atoms with at the most one bond to halogen, e.g. ester or nitrile radicals, directly attached in position 2
    • C07D277/70—Sulfur atoms
    • C07D277/72—2-Mercaptobenzothiazole
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D277/00—Heterocyclic compounds containing 1,3-thiazole or hydrogenated 1,3-thiazole rings
    • C07D277/60—Heterocyclic compounds containing 1,3-thiazole or hydrogenated 1,3-thiazole rings condensed with carbocyclic rings or ring systems
    • C07D277/62—Benzothiazoles
    • C07D277/68—Benzothiazoles with hetero atoms or with carbon atoms having three bonds to hetero atoms with at the most one bond to halogen, e.g. ester or nitrile radicals, directly attached in position 2
    • C07D277/70—Sulfur atoms
    • C07D277/74—Sulfur atoms substituted by carbon atoms
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D417/00—Heterocyclic compounds containing two or more hetero rings, at least one ring having nitrogen and sulfur atoms as the only ring hetero atoms, not provided for by group C07D415/00
    • C07D417/02—Heterocyclic compounds containing two or more hetero rings, at least one ring having nitrogen and sulfur atoms as the only ring hetero atoms, not provided for by group C07D415/00 containing two hetero rings
    • C07D417/04—Heterocyclic compounds containing two or more hetero rings, at least one ring having nitrogen and sulfur atoms as the only ring hetero atoms, not provided for by group C07D415/00 containing two hetero rings directly linked by a ring-member-to-ring-member bond
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D417/00—Heterocyclic compounds containing two or more hetero rings, at least one ring having nitrogen and sulfur atoms as the only ring hetero atoms, not provided for by group C07D415/00
    • C07D417/02—Heterocyclic compounds containing two or more hetero rings, at least one ring having nitrogen and sulfur atoms as the only ring hetero atoms, not provided for by group C07D415/00 containing two hetero rings
    • C07D417/06—Heterocyclic compounds containing two or more hetero rings, at least one ring having nitrogen and sulfur atoms as the only ring hetero atoms, not provided for by group C07D415/00 containing two hetero rings linked by a carbon chain containing only aliphatic carbon atoms
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D417/00—Heterocyclic compounds containing two or more hetero rings, at least one ring having nitrogen and sulfur atoms as the only ring hetero atoms, not provided for by group C07D415/00
    • C07D417/14—Heterocyclic compounds containing two or more hetero rings, at least one ring having nitrogen and sulfur atoms as the only ring hetero atoms, not provided for by group C07D415/00 containing three or more hetero rings
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6486—Measuring fluorescence of biological material, e.g. DNA, RNA, cells
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G01N2021/6439—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" with indicators, stains, dyes, tags, labels, marks
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2201/00—Features of devices classified in G01N21/00
    • G01N2201/06—Illumination; Optics
    • G01N2201/061—Sources
    • G01N2201/06113—Coherent sources; lasers
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/14—Heterocyclic carbon compound [i.e., O, S, N, Se, Te, as only ring hetero atom]
    • Y10T436/142222—Hetero-O [e.g., ascorbic acid, etc.]
    • Y10T436/143333—Saccharide [e.g., DNA, etc.]
JP2008511465A 2005-05-11 2006-05-11 ２本鎖ｄｎａへの高い選択性を有する蛍光化学物質及びそれらの使用 Active JP5306811B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US68024305P 2005-05-11 2005-05-11
US60/680,243 2005-05-11
PCT/US2006/018801 WO2006124816A1 (en) 2005-05-11 2006-05-11 Fluorescent chemical compounds having high selectivity for double stranded dna, and methods for their use

Related Child Applications (1)

Application Number Title Priority Date Filing Date
JP2012239245A Division JP5828828B2 (ja) 2005-05-11 2012-10-30 ２本鎖ｄｎａへの高い選択性を有する蛍光化学物質及びそれらの使用

Publications (2)

Publication Number Publication Date
JP2008540578A JP2008540578A (ja) 2008-11-20
JP5306811B2 true JP5306811B2 (ja) 2013-10-02

Family

ID=37431576

Family Applications (2)

Application Number Title Priority Date Filing Date
JP2008511465A Active JP5306811B2 (ja) 2005-05-11 2006-05-11 ２本鎖ｄｎａへの高い選択性を有する蛍光化学物質及びそれらの使用
JP2012239245A Expired - Fee Related JP5828828B2 (ja) 2005-05-11 2012-10-30 ２本鎖ｄｎａへの高い選択性を有する蛍光化学物質及びそれらの使用

Family Applications After (1)

Application Number Title Priority Date Filing Date
JP2012239245A Expired - Fee Related JP5828828B2 (ja) 2005-05-11 2012-10-30 ２本鎖ｄｎａへの高い選択性を有する蛍光化学物質及びそれらの使用

Country Status (4)

Country Link
US (7) US7598390B2 (US07943777-20110517-C00097.png)
EP (1) EP1885718B1 (US07943777-20110517-C00097.png)
JP (2) JP5306811B2 (US07943777-20110517-C00097.png)
WO (1) WO2006124816A1 (US07943777-20110517-C00097.png)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2013060448A (ja) * 2005-05-11 2013-04-04 Molecular Probes Inc ２本鎖ｄｎａへの高い選択性を有する蛍光化学物質及びそれらの使用

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7776529B2 (en) 2003-12-05 2010-08-17 Life Technologies Corporation Methine-substituted cyanine dye compounds
JP2009524832A (ja) * 2006-01-24 2009-07-02 ライフ テクノロジーズ コーポレーション 検体を定量するためのデバイスおよび方法
FR2904833A1 (fr) * 2006-08-11 2008-02-15 Bioquanta Sarl Procede de dosage d'acide nuclieque par fluorescence
ATE479779T1 (de) * 2006-09-29 2010-09-15 Leukocare Ag Verfahren zum nachweis einer aktivierung des immunsystems oder des ausmasses von zelltod
DE102008022221A1 (de) * 2008-05-06 2009-11-12 Universität des Saarlandes Inhibitoren der humanen Aldosteronsynthase CYP11B2
CN102154003B (zh) * 2011-02-12 2015-04-22 天津城市建设学院 咔唑桥基新型荧光菁染料探针及其制备方法
EP2820157B1 (en) 2012-03-02 2019-05-01 Winthrop-University Hospital Method for using probe based pcr detection to measure the levels of circulating demethylated beta cell derived dna as a measure of beta cell loss in diabetes
CN104059376B (zh) * 2014-07-01 2015-12-30 浙江大学 一种不对称菁染料化合物及其应用
WO2022006368A2 (en) 2020-07-02 2022-01-06 Life Technologies Corporation Trinucleotide cap analogs, preparation and uses thereof
WO2022099658A1 (zh) * 2020-11-13 2022-05-19 大连理工大学 菁类化合物、含菁类化合物的染料以及菁类化合物的应用

Family Cites Families (218)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US2269234A (en) 1940-10-18 1942-01-06 Eastman Kodak Co Polymethine dye intermediates
DE917330C (de) 1940-10-22 1955-01-10 Agfa Ag Fuer Photofabrikation Verfahren zur Herstellung von am mittelstaendigen Kohlenstoffatom der Methinkette substituierten, symmetrischen und unsymmetrischen Carbocyaninen
BE477314A (US07943777-20110517-C00097.png) * 1946-11-22
BE502441A (US07943777-20110517-C00097.png) * 1950-04-08
GB870753A (en) 1957-07-04 1961-06-21 Ilford Ltd Improvements in or relating to methine dyes
GB1053082A (US07943777-20110517-C00097.png) 1963-06-10
US3262153A (en) 1964-07-13 1966-07-26 Du Pont Apparatus for producing composite filaments
US3458318A (en) * 1965-08-02 1969-07-29 Eastman Kodak Co Supersensitized silver halide emulsions
BE691230A (US07943777-20110517-C00097.png) 1965-12-27 1967-05-16
US3490910A (en) * 1968-02-02 1970-01-20 Gaf Corp Silver halide emulsions sensitized with cyanine dyes containing a tetrahydrobenzo-benzoxazole nucleus
DE1805548A1 (de) 1968-10-26 1970-10-08 Agfa Gevaert Ag Benzimidazol-Derivate und ihre photographische Verwendung
DE2008114A1 (de) * 1970-02-21 1971-09-02 Agfa Gevaert AG, 5090 Leverkusen Spektral sensibihsiertes hchtempfind liches Material
US3988513A (en) 1970-07-06 1976-10-26 Fuji Photo Film Co., Ltd. Silver halide emulsions for recording electron rays
JPS4841205B1 (US07943777-20110517-C00097.png) 1970-07-16 1973-12-05
JPS5121340B1 (US07943777-20110517-C00097.png) 1971-03-23 1976-07-01
JPS5434330B2 (US07943777-20110517-C00097.png) 1972-04-12 1979-10-26
US4003750A (en) 1973-05-03 1977-01-18 Eastman Kodak Company Silver halide emulsion containing photographic sensitizing dyes
GB1529202A (en) 1976-02-20 1978-10-18 Ciba Geigy Ag Spectral sensitising dyes
US4126516A (en) 1976-11-29 1978-11-21 Corning Glass Works Method for distinguishing gram positive and gram negative microbes
US4343782A (en) 1978-04-20 1982-08-10 Shapiro Howard M Cytological assay procedure
US4225669A (en) 1979-04-27 1980-09-30 Melnick Joseph L Staining and analysis of bacteria
US4424201A (en) 1978-11-28 1984-01-03 Rockefeller University Employment of a mereyanine dye for the detection of malignant leukocytic cells
US4190328A (en) 1978-12-01 1980-02-26 Levine Robert A Process for detection of blood-borne parasites
JPS55116259A (en) 1979-03-01 1980-09-06 Fuji Photo Film Co Ltd Microimmunoassay method
JPS56110927A (en) 1980-02-07 1981-09-02 Mitsubishi Paper Mills Ltd Manufacture of silver halide photographic material
US4544546A (en) 1980-04-21 1985-10-01 Abbott Laboratories Fluorescent nucleic acid stains
CA1155041A (en) 1980-04-21 1983-10-11 Michael E. Jolley Fluorescent nucleic acid stains
US4304908A (en) 1980-06-23 1981-12-08 Eastman Kodak Company Methine colorant materials and the use thereof in electrophoretic migration imaging layers and processes
JPS6310515Y2 (US07943777-20110517-C00097.png) 1980-10-17 1988-03-29
JPS5772150A (en) 1980-10-23 1982-05-06 Ishihara Sangyo Kaisha Ltd Electrophotographic sensitive material
US4711955A (en) 1981-04-17 1987-12-08 Yale University Modified nucleotides and methods of preparing and using same
US4520110A (en) 1981-10-06 1985-05-28 The Board Of Trustees Of The Leland Stanford Junior University Fluorescent immunoassay employing a phycobiliprotein labeled ligand or receptor
US4859582A (en) 1981-10-06 1989-08-22 The Board Of Trustees Of The Leland Stanford Jr. University Fluorescent conjugates for analysis of molecules and cells
US5055556A (en) 1981-10-06 1991-10-08 The Board Of Trustees Of The Leland Stanford Jr. Univ. Fluorescent conjugates for analysis of molecules and cells
FR2517071A1 (fr) 1981-11-24 1983-05-27 Commissariat Energie Atomique Recepteur pour sondeur de detection et de mesure de phenomenes relatifs a l'environnement du globe terrestre
US4771955A (en) 1982-04-29 1988-09-20 Paulson John K Pepper mill closure
US4542104A (en) 1983-04-06 1985-09-17 The Board Of Trustees Of The Leland Stanford Jr. Univ. Phycobiliprotein fluorescent conjugates
US4510235A (en) 1983-04-28 1985-04-09 Fuji Photo Film Co., Ltd. Silver halide photographic emulsions
US4556636A (en) 1983-06-09 1985-12-03 Eastman Kodak Company Composition, analytical element and method for the detection of bacteria
US4508821A (en) 1983-07-05 1985-04-02 Becton Dickinson And Company Detection of white cell associated bacteria with a fluorescent dye
US4737454A (en) 1983-07-14 1988-04-12 Molecular Diagnostics, Inc. Fast photochemical method of labelling nucleic acids for detection purposes in hybridization assays
US5171534A (en) 1984-01-16 1992-12-15 California Institute Of Technology Automated DNA sequencing technique
US4603209A (en) 1984-09-07 1986-07-29 The Regents Of The University Of California Fluorescent indicator dyes for calcium ions
US4639421A (en) 1984-10-01 1987-01-27 Becton, Dickinson And Company Fluorescent gram stain
US4665024A (en) 1984-10-01 1987-05-12 Becton, Dickinson And Company Fluorescent gram stain
US5221518A (en) 1984-12-14 1993-06-22 Mills Randell L DNA sequencing apparatus
US4886744A (en) 1985-04-25 1989-12-12 Polaroid Corporation Fluorescent conjugates and biological diagnostic assay system
US4740891A (en) 1985-05-06 1988-04-26 Tektronix, Inc. Asynchronous state machine
EP0218385B2 (en) 1985-09-17 1997-05-14 Konica Corporation Thermally developable light-sensitive material
DE3677916D1 (de) 1985-11-01 1991-04-11 Becton Dickinson Co Nachweis von retikulozyten.
US4957870A (en) 1985-11-01 1990-09-18 Becton, Dickinson And Company Detection of Reticulocytes, RNA and DNA
US4883867A (en) 1985-11-01 1989-11-28 Becton, Dickinson And Company Detection of reticulocytes, RNA or DNA
US4812409A (en) 1986-01-31 1989-03-14 Eastman Kodak Company Hydrolyzable fluorescent substrates and analytical determinations using same
US6225050B1 (en) * 1986-04-18 2001-05-01 Carnegie Mellon University Cyanine dyes as labeling reagents for detection of biological and other materials by luminescence methods
US5268486A (en) 1986-04-18 1993-12-07 Carnegie-Mellon Unversity Method for labeling and detecting materials employing arylsulfonate cyanine dyes
US5569587A (en) 1986-04-18 1996-10-29 Carnegie Mellon University Method for labeling and detecting materials employing luminescent arysulfonate cyanine dyes
US6048982A (en) * 1986-04-18 2000-04-11 Carnegie Mellon University Cyanine dyes as labeling reagents for detection of biological and other materials by luminescence methods
US5627027A (en) * 1986-04-18 1997-05-06 Carnegie Mellon University Cyanine dyes as labeling reagents for detection of biological and other materials by luminescence methods
US5047519A (en) 1986-07-02 1991-09-10 E. I. Du Pont De Nemours And Company Alkynylamino-nucleotides
CA1340806C (en) 1986-07-02 1999-11-02 James Merrill Prober Method, system and reagents for dna sequencing
US4859584A (en) 1986-10-31 1989-08-22 Smithkline Beckman Corporation Cell growth rate determination by measurement of changes in cyanine dye levels in plasma membranes
US4762701A (en) 1986-10-31 1988-08-09 Smithkline Beckman Corporation In vivo cellular tracking
US4783401A (en) 1986-10-31 1988-11-08 Smithkline Beckman Corporation Viable cell labelling
US4810636A (en) 1986-12-09 1989-03-07 Miles Inc. Chromogenic acridinone enzyme substrates
JPS63132688U (US07943777-20110517-C00097.png) 1987-02-24 1988-08-30
US4921805A (en) 1987-07-29 1990-05-01 Life Technologies, Inc. Nucleic acid capture method
US4945171A (en) 1987-08-10 1990-07-31 Molecular Probes, Inc. Xanthene dyes having a fused (C) benzo ring
US4774339A (en) 1987-08-10 1988-09-27 Molecular Probes, Inc. Chemically reactive dipyrrometheneboron difluoride dyes
US5049673A (en) 1987-10-30 1991-09-17 The Regents Of The University Of California Fluorescent indicator dyes for calcium working at long wavelengths
US4849362A (en) 1988-05-19 1989-07-18 Smithkline Beckman Corporation Fluorescent intracellular calcium indicators
US5057413A (en) 1988-06-13 1991-10-15 Becton, Dickinson And Company Method for discriminating between intact and damaged cells in a sample
US4997928A (en) 1988-09-15 1991-03-05 E. I. Du Pont De Nemours And Company Fluorescent reagents for the preparation of 5'-tagged oligonucleotides
EP0367540A2 (en) 1988-11-01 1990-05-09 Konica Corporation Silver halide photographic material
JPH0284383U (US07943777-20110517-C00097.png) 1988-12-15 1990-06-29
US5122602A (en) 1989-02-13 1992-06-16 Miles Inc. Chromogenic merocyanine enzyme substrates
US5401469A (en) 1989-04-19 1995-03-28 Ibiden Co., Ltd. Plastic optical biomaterials assay device
US5137810A (en) 1989-04-26 1992-08-11 The University Of North Carolina Method of determining the gram sign of bacteria
US5169788A (en) 1989-06-09 1992-12-08 New England Deaconess Hospital Corporation Methods of measuring membrane potential using j-aggregate forming dyes
US4937198A (en) 1989-07-28 1990-06-26 Becton, Dickinson And Company Novel fluorescent dye
ATA194989A (de) 1989-08-17 1990-04-15 Koller Ernst Verfahren zum nachweis und zur bestimmung von humanserumalbumin in biologischen fluessigkeiten mit hilfe fluorogener cyanin-farbstoffe
US5132432A (en) 1989-09-22 1992-07-21 Molecular Probes, Inc. Chemically reactive pyrenyloxy sulfonic acid dyes
US5375606A (en) 1990-02-28 1994-12-27 Zynaxis, Inc. Bio-analytical separation method
US5401847A (en) 1990-03-14 1995-03-28 Regents Of The University Of California DNA complexes with dyes designed for energy transfer as fluorescent markers
US5763162A (en) 1990-03-14 1998-06-09 The Regents Of University Of California Multichromophore fluorescent DNA intercalation complexes
US5312921A (en) * 1990-03-14 1994-05-17 Regents Of The University Of California Dyes designed for high sensitivity detection of double-stranded DNA
US5648270A (en) * 1995-02-06 1997-07-15 Molecular Probes, Inc. Methods of sensing with fluorescent conjugates of metal-chelating nitrogen heterocycles
US5501980A (en) 1994-05-20 1996-03-26 Molecular Probes, Inc. Benzazolylcoumarin-based ion indicators
US5405975A (en) 1993-03-29 1995-04-11 Molecular Probes, Inc. Fluorescent ion-selective diaryldiaza crown ether conjugates
US5453517A (en) 1992-02-25 1995-09-26 Molecular Probes, Inc. Reactive derivatives of bapta used to make ion-selective chelators
US5227487A (en) 1990-04-16 1993-07-13 Molecular Probes, Inc. Certain tricyclic and pentacyclic-hetero nitrogen rhodol dyes
US5459276A (en) 1994-05-20 1995-10-17 Molecular Probes, Inc. Benzazolylcoumarin-based ion indicators for heavy metals
US5433896A (en) 1994-05-20 1995-07-18 Molecular Probes, Inc. Dibenzopyrrometheneboron difluoride dyes
US5326692B1 (en) 1992-05-13 1996-04-30 Molecular Probes Inc Fluorescent microparticles with controllable enhanced stokes shift
US5274113A (en) 1991-11-01 1993-12-28 Molecular Probes, Inc. Long wavelength chemically reactive dipyrrometheneboron difluoride dyes and conjugates
GB9008818D0 (en) 1990-04-19 1990-06-13 Ici Plc Amine derivatives
US5714327A (en) 1990-07-19 1998-02-03 Kreatech Diagnostics Platinum-containing compounds, methods for their preparation and applications thereof
US5426772A (en) 1990-07-27 1995-06-20 Intel Corporation Single PAL circuit generating system clock and control signals to minimize skew
DE59108978D1 (de) 1990-08-28 1998-06-10 Landis & Gyr Tech Innovat Verfahren zum Aendern einer in einem Computer eines Gerätes abgespeicherten Maschinensprachenfassung eines ersten Programms in eine Maschinensprachenfassung eines durch mindestens eine Aenderung vom ersten Programm abgeleiteten zweiten Programms
US5248672A (en) 1990-11-01 1993-09-28 The Regents Of The University Of Michigan Polysubstituted benzimidazole nucleosides as antiviral agents
US5208148A (en) 1990-12-07 1993-05-04 Molecular Probes, Inc. Lipophilic fluorescent glycosidase substrates
US5248782A (en) 1990-12-18 1993-09-28 Molecular Probes, Inc. Long wavelength heteroaryl-substituted dipyrrometheneboron difluoride dyes
DE69132765T2 (de) 1990-12-24 2002-02-21 Enzo Diagnostics Inc Verfahren zum Nachweis eines Zielpolynukleotids in einer Probe unter Verwendung eines Reagenz, das den Hintergrund verringert, und eine dieses Reagenz enthaltende Zusammensetzung und Kit.
US5451343A (en) 1991-05-20 1995-09-19 Spectra Group Limited, Inc. Fluorone and pyronin y derivatives
US5187288A (en) 1991-05-22 1993-02-16 Molecular Probes, Inc. Ethenyl-substituted dipyrrometheneboron difluoride dyes and their synthesis
EP0517050B1 (en) 1991-06-06 1996-09-18 Bayer Corporation Test-strip containing merocyanine and nitro or nitroso substituted polyhalogenated phenol-sulfonephthaleins as protein indicators
US5264589A (en) 1991-06-06 1993-11-23 Miles Inc. Merocyanine protein error indicators
US5193180A (en) 1991-06-21 1993-03-09 Pure Software Inc. System for modifying relocatable object code files to monitor accesses to dynamically allocated memory
JP2802693B2 (ja) * 1991-08-14 1998-09-24 富士写真フイルム株式会社 ハロゲン化銀乳剤
US5242805A (en) 1991-08-23 1993-09-07 Molecular Probes, Inc. Long wavelength lipophilic fluorogenic glycosidase substrates
US5316906A (en) 1991-08-23 1994-05-31 Molecular Probes, Inc. Enzymatic analysis using substrates that yield fluorescent precipitates
ATE180018T1 (de) 1991-08-23 1999-05-15 Molecular Probes Inc Verwendung von haloalkylderivaten von reportermolekülen zur analyse der metabolischen aktivität in zellen
DE69219610T2 (de) 1991-09-16 1997-10-02 Molecular Probes Inc Dimere unsymmetrische Cyaninfarbstoffe
US5314805A (en) 1991-10-28 1994-05-24 Molecular Probes, Inc. Dual-fluorescence cell viability assay using ethidium homodimer and calcein AM
WO1993011120A1 (en) 1991-11-27 1993-06-10 Zynaxis Technologies, Incorporated Compounds, compositions and methods for binding bio-affecting substances to surface membranes of bio-particles
US5321130A (en) * 1992-02-10 1994-06-14 Molecular Probes, Inc. Unsymmetrical cyanine dyes with a cationic side chain
US5352803A (en) 1992-03-30 1994-10-04 Abbott Laboratories 5(6)-methyl substituted fluorescein derivatives
JPH05287209A (ja) 1992-04-09 1993-11-02 Hitachi Chem Co Ltd 蛍光標識用色素、蛍光標識用色素で標識された生物由来物質、及びそれらを含有する試薬
EP0658167B1 (en) 1992-09-03 1999-04-14 The Perkin-Elmer Corporation 4,7-dichlorofluorescein dyes as molecular probes
JPH06123740A (ja) 1992-10-12 1994-05-06 Canon Inc 蛋白質標識用（メロ）シアニン色素、及び色素標識蛋白質
US5410030A (en) 1993-04-05 1995-04-25 Molecular Probes, Inc. Dimers of unsymmetrical cyanine dyes containing pyridinium moieties
US5445946A (en) 1993-04-13 1995-08-29 Molecular Probes, Inc. Intravacuolar stains for yeast and other fungi
US5534416A (en) 1993-04-13 1996-07-09 Molecular Probes, Inc. Fluorescent viability assay using cyclic-substituted unsymmetrical cyanine dyes
US5436134A (en) 1993-04-13 1995-07-25 Molecular Probes, Inc. Cyclic-substituted unsymmetrical cyanine dyes
US5545535A (en) * 1993-04-13 1996-08-13 Molecular Probes, Inc. Fluorescent assay for bacterial gram reaction
US5658751A (en) * 1993-04-13 1997-08-19 Molecular Probes, Inc. Substituted unsymmetrical cyanine dyes with selected permeability
US5437980A (en) 1993-05-17 1995-08-01 Molecular Probes, Inc. Phenanthridium dye staining of nucleic acids in living cells
US5426722A (en) 1993-09-09 1995-06-20 Stratasys, Inc. Method for optimizing the motion of a multi-axis robot
US5459268A (en) 1993-10-25 1995-10-17 Molecular Probes, Inc. Xanthylium dyes that are well retained in mitochondria
US5925517A (en) * 1993-11-12 1999-07-20 The Public Health Research Institute Of The City Of New York, Inc. Detectably labeled dual conformation oligonucleotide probes, assays and kits
US5840493A (en) 1994-03-30 1998-11-24 Mitokor Mitochondrial DNA mutations that segregate with late onset diabetes mellitus
US5597696A (en) * 1994-07-18 1997-01-28 Becton Dickinson And Company Covalent cyanine dye oligonucleotide conjugates
JP3494317B2 (ja) 1994-07-29 2004-02-09 富士写真フイルム株式会社 ハロゲン化銀写真感光材料
US5565554A (en) 1994-07-29 1996-10-15 The Regents Of The University Of California Dimeric fluorescent energy transfer dyes comprising asymmetric cyanine azole-indolenine chromophores
US5656554A (en) 1994-07-29 1997-08-12 International Business Machines Corporation Semiconductor chip reclamation technique involving multiple planarization processes
DE69526617T2 (de) 1994-09-22 2002-08-29 Fuji Photo Film Co Ltd Infrarot absorbierenden Farbstoff photographisches Silberhalogenidmaterial
US5564554A (en) 1994-11-01 1996-10-15 Lawrence Equipment, Inc. Reciprocating accumulation conveyor
JP3189000B2 (ja) 1994-12-01 2001-07-16 東ソー株式会社 特定核酸配列の検出方法
US5656449A (en) * 1995-03-06 1997-08-12 Molecular Probes, Inc. Neutral unsymmetrical cyanine dyes
US6342389B1 (en) * 1995-04-10 2002-01-29 Roger S. Cubicciotti Modified phycobilisomes and uses therefore
US5616502A (en) * 1995-05-19 1997-04-01 Molecular Probes, Inc. Non-specific protein staining using merocyanine dyes
US6008373A (en) 1995-06-07 1999-12-28 Carnegie Mellon University Fluorescent labeling complexes with large stokes shift formed by coupling together cyanine and other fluorochromes capable of resonance energy transfer
US5798276A (en) 1995-06-07 1998-08-25 Molecular Probes, Inc. Reactive derivatives of sulforhodamine 101 with enhanced hydrolytic stability
US5929227A (en) * 1995-07-12 1999-07-27 The Regents Of The University Of California Dimeric fluorescent energy transfer dyes comprising asymmetric cyanine azole-indolenine chromophores
GB9514525D0 (en) 1995-07-15 1995-09-13 Philips Electronics Uk Ltd Data packet handling
US6021272A (en) 1995-10-04 2000-02-01 Platinum Technology, Inc. Transforming and manipulating program object code
US5869689A (en) * 1995-10-17 1999-02-09 Molecular Probes, Inc Stains for acidic organelles
US5658735A (en) 1995-11-09 1997-08-19 Biometric Imaging, Inc. Cyclized fluorescent nucleic acid intercalating cyanine dyes and nucleic acid detection methods
US5734058A (en) * 1995-11-09 1998-03-31 Biometric Imaging, Inc. Fluorescent DNA-Intercalating cyanine dyes including a positively charged benzothiazole substituent
US6291203B1 (en) 1995-11-13 2001-09-18 Molecular Probes, Inc. Cyanine dyes that stain cells and mitochondria
US6004536A (en) 1995-11-14 1999-12-21 Molecular Probes, Inc. Lipophilic cyanine dyes with enchanced aqueous solubilty
JPH09218495A (ja) 1996-02-14 1997-08-19 Fuji Photo Film Co Ltd 画像形成方法
US6162931A (en) 1996-04-12 2000-12-19 Molecular Probes, Inc. Fluorinated xanthene derivatives
JPH09281495A (ja) 1996-04-17 1997-10-31 Seiko Instr Kk 液晶表示パネル及びその製造方法
DE69706417T2 (de) 1996-04-19 2002-06-27 Amersham Pharm Biotech Uk Ltd Quadratfarbstoffe und deren verwendung in einem fluorescenzsequenzierungsverfahren
US6153370A (en) 1996-04-25 2000-11-28 Fuji Photo Film Co., Ltd. Silver halide emulsion and silver halide light-sensitive material using the same
EP1111451A3 (en) 1996-04-30 2004-11-17 Fuji Photo Film Co., Ltd. Photothermographic material
US6083699A (en) * 1996-05-01 2000-07-04 Visible Genetics Inc. Method for bi-directional sequencing of nucleic acid polymers
US5847162A (en) * 1996-06-27 1998-12-08 The Perkin Elmer Corporation 4, 7-Dichlororhodamine dyes
US5800996A (en) 1996-05-03 1998-09-01 The Perkin Elmer Corporation Energy transfer dyes with enchanced fluorescence
US5863727A (en) * 1996-05-03 1999-01-26 The Perkin-Elmer Corporation Energy transfer dyes with enhanced fluorescence
US5945526A (en) 1996-05-03 1999-08-31 Perkin-Elmer Corporation Energy transfer dyes with enhanced fluorescence
SE506700C2 (sv) 1996-05-31 1998-02-02 Mikael Kubista Sond och förfaranden för analys av nukleinsyra
US6080852A (en) * 1996-06-27 2000-06-27 The Perkin-Elmer Corporation 4,7-dichlororhodamine dyes
US6017712A (en) * 1996-06-27 2000-01-25 Lee; Linda 4,7-dichlororhodamine dyes
US5846737A (en) 1996-07-26 1998-12-08 Molecular Probes, Inc. Conjugates of sulforhodamine fluorophores with enhanced fluorescence
US5691145A (en) 1996-08-27 1997-11-25 Becton, Dickinson And Company Detection of nucleic acids using G-quartets
US5830912A (en) 1996-11-15 1998-11-03 Molecular Probes, Inc. Derivatives of 6,8-difluoro-7-hydroxycoumarin
US5696157A (en) 1996-11-15 1997-12-09 Molecular Probes, Inc. Sulfonated derivatives of 7-aminocoumarin
US6495692B1 (en) * 1996-12-10 2002-12-17 Abbott Laboratories Helium-neon excitable reticulocyte dyes derivable from halolepidines
US6027709A (en) * 1997-01-10 2000-02-22 Li-Cor Inc. Fluorescent cyanine dyes
JP3579228B2 (ja) 1997-01-24 2004-10-20 大日本スクリーン製造株式会社 基板処理装置
EP0870753A1 (en) 1997-04-04 1998-10-14 Imperial Chemical Industries Plc A process for the preparation of methacrylate esters
JP3783808B2 (ja) * 1997-05-19 2006-06-07 シスメックス株式会社 白血球分類計数用試薬
US6348599B1 (en) * 1997-07-28 2002-02-19 Nycomed Amersham Plc Cyanine dyes
US6130101A (en) 1997-09-23 2000-10-10 Molecular Probes, Inc. Sulfonated xanthene derivatives
US6008379A (en) 1997-10-01 1999-12-28 The Perkin-Elmer Corporation Aromatic-substituted xanthene dyes
US6133429A (en) * 1997-10-03 2000-10-17 Becton Dickinson And Company Chromophores useful for the preparation of novel tandem conjugates
US5936087A (en) * 1997-11-25 1999-08-10 The Perkin-Elmer Corporation Dibenzorhodamine dyes
US6200752B1 (en) * 1998-01-14 2001-03-13 Joseph R. Lakowicz Method and composition for detecting the presence of a nucleic acid sequence in a sample
US6080868A (en) * 1998-01-23 2000-06-27 The Perkin-Elmer Corporation Nitro-substituted non-fluorescent asymmetric cyanine dye compounds
DE19824535A1 (de) * 1998-06-03 1999-12-09 Roche Diagnostics Gmbh Neue Rhodamin-Derivate und deren Verwendung
GB9812596D0 (en) 1998-06-11 1998-08-12 Amersham Pharm Biotech Uk Ltd Energy transfer assay method
US6331385B1 (en) 1998-09-11 2001-12-18 Eastman Kodak Company Photographic material having enhanced light absorption
US6271035B1 (en) * 1998-10-20 2001-08-07 Coulter International Corp. Methods and compositions for rapid staining of nucleic acids in whole cells
US6316276B1 (en) 1998-12-17 2001-11-13 Lsi Lgoic Corporation Apparatus and method of planarizing a semiconductor wafer that includes a first reflective substance and a second reflective substance
JP2000241932A (ja) * 1998-12-21 2000-09-08 Konica Corp 熱現像写真感光材料、非感光性銀塩化合物の製造方法および画像形成方法
JP3983404B2 (ja) * 1999-01-13 2007-09-26 本田技研工業株式会社 レーダ搭載車両用ゲート
JP4360575B2 (ja) 1999-03-01 2009-11-11 富士フイルム株式会社 液晶化合物、液晶混合物、液晶組成物、電解質、電気化学電池および光電気化学電池
US6114350A (en) 1999-04-19 2000-09-05 Nen Life Science Products, Inc. Cyanine dyes and synthesis methods thereof
DE60001531T2 (de) * 1999-04-23 2003-10-02 Molecular Probes Inc Xanthenfarbstoffe und ihre anwendung als lumineszenzlöschende verbindungen
US6197959B1 (en) * 1999-04-27 2001-03-06 Hoffmann-La Roche Inc. Piperidine derivatives
US6664047B1 (en) * 1999-04-30 2003-12-16 Molecular Probes, Inc. Aza-benzazolium containing cyanine dyes
EP1055734B1 (en) * 1999-05-24 2004-10-13 Tosoh Corporation Method for assaying ribonucleic acid
WO2001002374A1 (en) * 1999-07-06 2001-01-11 Surromed, Inc. Bridged fluorescent dyes, their preparation and their use in assays
US6358684B1 (en) * 1999-08-27 2002-03-19 Pe Corporation UV excitable fluorescent energy transfer dyes
US6329205B1 (en) 1999-08-31 2001-12-11 Molecular Probes, Inc. Detection method using luminescent europium-based protein stains
US6239626B1 (en) * 2000-01-07 2001-05-29 Cisco Technology, Inc. Glitch-free clock selector
US6365341B1 (en) * 2000-03-24 2002-04-02 Biowhittaker Molecular Applications, Inc. Stabilization of highly sensitive nucleic acid stains in aqueous solutions
US6368801B1 (en) * 2000-04-12 2002-04-09 Molecular Staging, Inc. Detection and amplification of RNA using target-mediated ligation of DNA by RNA ligase
US6368864B1 (en) 2000-05-05 2002-04-09 Coulter International Corp. Dyes and methods of reticulocyte enumeration
US6323337B1 (en) 2000-05-12 2001-11-27 Molecular Probes, Inc. Quenching oligonucleotides
US6579718B1 (en) * 2000-08-04 2003-06-17 Molecular Probes, Inc. Carbazolylvinyl dye protein stains
ATE415399T1 (de) * 2000-08-04 2008-12-15 Molecular Probes Inc Kondensierte ringe enthaltende 1,2-dihydro-7- hydroxychinolinderivate
ATE352586T2 (de) 2000-09-29 2007-02-15 Molecular Probes Inc Modifizierte carbocyaninfarbstoffe und deren konjugate
AU2001296502B2 (en) 2000-10-02 2005-06-09 Molecular Probes, Inc. Reagents for labeling biomolecules having aldehyde or ketone moieties
SE519116C2 (sv) * 2001-05-10 2003-01-14 Lightup Technologies Ab Syntes och utvärdering av nya cyaninfärgämnen som binder till minor groove
US6972326B2 (en) 2001-12-03 2005-12-06 Molecular Probes, Inc. Labeling of immobilized proteins using dipyrrometheneboron difluoride dyes
IL150501A0 (en) 2002-07-01 2003-02-12 Elazar Shachor Method for wagering
US7582429B2 (en) 2002-10-23 2009-09-01 University Of Utah Research Foundation Amplicon melting analysis with saturation dyes
DE10329019A1 (de) 2003-06-27 2005-01-13 Daimlerchrysler Ag Brennkraftmaschine mit einem Verdichter im Ansaugtrakt und Verfahren hierzu
US20050074796A1 (en) 2003-07-31 2005-04-07 Stephen Yue Unsymmetrical cyanine dimer compounds and their application
US7271265B2 (en) * 2003-08-11 2007-09-18 Invitrogen Corporation Cyanine compounds and their application as quenching compounds
EP1720944B1 (en) * 2003-12-05 2013-07-17 Life Technologies Corporation Cyanine dye compounds
US7776529B2 (en) 2003-12-05 2010-08-17 Life Technologies Corporation Methine-substituted cyanine dye compounds
EP2610315B1 (en) 2003-12-05 2016-08-31 Life Technologies Corporation Methine-substituted cyanine dye compounds
WO2005083394A2 (en) 2004-02-20 2005-09-09 Molecular Probes, Inc. Methods for detecting anionic and non-anionic proteins using carbocyanine dyes
JP2006292808A (ja) * 2005-04-06 2006-10-26 Konica Minolta Medical & Graphic Inc 銀塩光熱写真ドライイメージング材料及び画像形成方法
US7598390B2 (en) 2005-05-11 2009-10-06 Life Technologies Corporation Fluorescent chemical compounds having high selectivity for double stranded DNA, and methods for their use

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2013060448A (ja) * 2005-05-11 2013-04-04 Molecular Probes Inc ２本鎖ｄｎａへの高い選択性を有する蛍光化学物質及びそれらの使用

Also Published As

Publication number Publication date
EP1885718A4 (en) 2014-10-29
JP2013060448A (ja) 2013-04-04
WO2006124816A1 (en) 2006-11-23
US20110217699A1 (en) 2011-09-08
US7943777B2 (en) 2011-05-17
EP1885718A1 (en) 2008-02-13
EP1885718B1 (en) 2017-03-15
US20150004597A1 (en) 2015-01-01
US20120288855A1 (en) 2012-11-15
US20100143917A1 (en) 2010-06-10
US9115397B2 (en) 2015-08-25
US20060263844A1 (en) 2006-11-23
US20150316558A1 (en) 2015-11-05
JP5828828B2 (ja) 2015-12-09
US8865904B2 (en) 2014-10-21
US20130224735A1 (en) 2013-08-29
US7598390B2 (en) 2009-10-06
US9366676B2 (en) 2016-06-14
JP2008540578A (ja) 2008-11-20

Similar Documents

Publication Publication Date Title
JP5828828B2 (ja) ２本鎖ｄｎａへの高い選択性を有する蛍光化学物質及びそれらの使用
Wang et al. Optical methods of antibiotic residues detections: A comprehensive review
Wu et al. A ratiometric fluorescent system for carboxylesterase detection with AIE dots as FRET donors
Zhou et al. A naphthalimide-indole fused chromophore-based fluorescent probe for instantaneous detection of thiophenol with a red emission and a large Stokes shift
JP5746135B2 (ja) 核酸結合色素およびその使用
JP6367307B2 (ja) 遺伝的同一性における使用のための新規ケイ素およびゲルマニウム色素
US9850382B1 (en) Unsymmetrical cyanine dyes and their application
KR101115014B1 (ko) 핵산 검출용 크로마토그래피 시스템
Zhang et al. New class of tetradentate β-diketonate-europium complexes that can be covalently bound to proteins for time-gated fluorometric application
JPH11323173A (ja) 二本鎖ｄｎａの高感度検出用染料
Lou et al. Detection of adenine-rich ssDNA based on thymine-substituted tetraphenylethene with aggregation-induced emission characteristics
KR20150098639A (ko) 메가스토크스 아미노-트리아졸릴-bodipy 화합물 및 살아있는 뉴런 염색 및 인간 혈청 알부민 fa1 약물 부위 프로빙에 대한 적용
CN111239391A (zh) 一种2019-nCoV新型冠状病毒抗原检测试剂及检测装置
CN106905389A (zh) 一种具有细胞内滞留能力的β‑半乳糖苷酶荧光探针
US8742114B2 (en) Nucleic acid detections and methods of their use
CN111675674A (zh) 一种aie分子及其合成方法
JP2007507238A5 (US07943777-20110517-C00097.png)
CN109320537A (zh) 一种用于面粉和活体内过氧苯甲酰检测的可溶性双光子荧光探针及其制备方法和应用
WO2019232895A1 (zh) 一种喹啉鎓盐型化合物及其应用
CN111239390A (zh) 一种新型抗原检测试剂及其制备方法
CN111153893A (zh) 一种用于检测细胞线粒体中so2衍生物的比率荧光探针及其应用
CN112114137A (zh) 一种新型特异性蛋白检测试剂及其制备方法
CN109824571B (zh) 一种荧光探针及其制备方法与应用
US20200263231A1 (en) Fluorogenic dyes for high sensitivity DNA detection
CN112898228A (zh) 基于混合组装的混合聚集体fret探针及其在检测线粒体自噬中的用途

Legal Events

Date Code Title Description
A621 Written request for application examination

Free format text: JAPANESE INTERMEDIATE CODE: A621

Effective date: 20090310

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20120521

A601 Written request for extension of time

Free format text: JAPANESE INTERMEDIATE CODE: A601

Effective date: 20120724

A602 Written permission of extension of time

Free format text: JAPANESE INTERMEDIATE CODE: A602

Effective date: 20120731

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20121030

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20130130

A711 Notification of change in applicant

Free format text: JAPANESE INTERMEDIATE CODE: A711

Effective date: 20130401

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20130403

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A821

Effective date: 20130401

TRDD Decision of grant or rejection written
A01 Written decision to grant a patent or to grant a registration (utility model)

Free format text: JAPANESE INTERMEDIATE CODE: A01

Effective date: 20130612

A61 First payment of annual fees (during grant procedure)

Free format text: JAPANESE INTERMEDIATE CODE: A61

Effective date: 20130626

R150 Certificate of patent or registration of utility model

Free format text: JAPANESE INTERMEDIATE CODE: R150

Ref document number: 5306811

Country of ref document: JP

Free format text: JAPANESE INTERMEDIATE CODE: R150

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250